WO2008027772A3 - Ultrafast immunoextraction/displacement assays - Google Patents

Ultrafast immunoextraction/displacement assays Download PDF

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Publication number
WO2008027772A3
WO2008027772A3 PCT/US2007/076541 US2007076541W WO2008027772A3 WO 2008027772 A3 WO2008027772 A3 WO 2008027772A3 US 2007076541 W US2007076541 W US 2007076541W WO 2008027772 A3 WO2008027772 A3 WO 2008027772A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
concentration
free
immunoextraction
ultrafast
Prior art date
Application number
PCT/US2007/076541
Other languages
French (fr)
Other versions
WO2008027772A2 (en
Inventor
David S Hage
Corey M Ohnmacht
Original Assignee
Univ Nebraska
David S Hage
Corey M Ohnmacht
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nebraska, David S Hage, Corey M Ohnmacht filed Critical Univ Nebraska
Publication of WO2008027772A2 publication Critical patent/WO2008027772A2/en
Publication of WO2008027772A3 publication Critical patent/WO2008027772A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9473—Anticonvulsants, e.g. phenobarbitol, phenytoin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

An analytical method for determining the concentration of an analyte or free analyte fraction in a sample that uses affinity chromatography in conjunction with labeled analyte analogs. In the method a sample containing an analyte is applied to an affinity column capable of selectively extracting the analyte in the millisecond time domain. The concentration of free analyte is then determined by a chromatographic affinity assay that correlates the analyte concentration to the quantity of labeled analyte competitively displaced from the column. The method of the invention is useful for determining concentrations of an analyte or a free analyte fraction in a single sample or in multiple samples on a short time-scale. Automation of such a method is further contemplated.
PCT/US2007/076541 2006-08-30 2007-08-22 Ultrafast immunoextraction/displacement assays WO2008027772A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82396406P 2006-08-30 2006-08-30
US60/823,964 2006-08-30

Publications (2)

Publication Number Publication Date
WO2008027772A2 WO2008027772A2 (en) 2008-03-06
WO2008027772A3 true WO2008027772A3 (en) 2008-12-18

Family

ID=39136728

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/076541 WO2008027772A2 (en) 2006-08-30 2007-08-22 Ultrafast immunoextraction/displacement assays

Country Status (1)

Country Link
WO (1) WO2008027772A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2022549560A (en) * 2019-09-20 2022-11-28 テルモ株式会社 Method for evaluating coating state of protein adsorbent or protein adsorption state
CN112014560A (en) * 2020-08-31 2020-12-01 复旦大学 Near-infrared fluorescence immunochromatographic test strip for detecting human anti-SARS-CoV-2 antibody

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5719027A (en) * 1993-02-05 1998-02-17 Canon Kabushiki Kaisha Labeled complex and method of analysis therewith
WO2002077644A1 (en) * 2001-02-05 2002-10-03 The Board Of Regents Of The University Of Nebraska Microcolumn chromatographic immunoassays
US20040053236A1 (en) * 2001-03-30 2004-03-18 Mccallum Claire M. Reverse genetic strategy for identifying functional mutations in genes of known sequences
US20050227258A1 (en) * 2003-12-08 2005-10-13 Bright Frank V Site selectively tagged and templated molecularly imprinted polymers for sensor applications

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5719027A (en) * 1993-02-05 1998-02-17 Canon Kabushiki Kaisha Labeled complex and method of analysis therewith
WO2002077644A1 (en) * 2001-02-05 2002-10-03 The Board Of Regents Of The University Of Nebraska Microcolumn chromatographic immunoassays
US20040053236A1 (en) * 2001-03-30 2004-03-18 Mccallum Claire M. Reverse genetic strategy for identifying functional mutations in genes of known sequences
US20050227258A1 (en) * 2003-12-08 2005-10-13 Bright Frank V Site selectively tagged and templated molecularly imprinted polymers for sensor applications

Also Published As

Publication number Publication date
WO2008027772A2 (en) 2008-03-06

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