WO2008026446A1

WO2008026446A1 - Method for extracting target substance and apparatus for extracting target substance

Info

Publication number: WO2008026446A1
Application number: PCT/JP2007/065858
Authority: WO
Inventors: Masahiro Yamagata; Motonobu Goto; Ryuichi Fukuzato
Original assignee: Kabushiki Kaisha Kobe Seiko Sho
Priority date: 2006-08-29
Filing date: 2007-08-14
Publication date: 2008-03-06
Also published as: JP2008055255A; JP4113230B2; CN101484224A; KR20090038470A

Abstract

It is intended to provide an extraction method capable of extracting a target substance selectively and effectively from a matter to be treated without resort to a multi-stage extraction process, and an extraction apparatus. The method for extracting a target substance of the invention comprises the steps of: bringing a high-pressure fluid mixture obtained by mixing a hydrophilic fluid component with a hydrophilic fluid component in a supercritical or subcritical state over their solubilities into contact with a solid matter to be treated containing a target substance, and extracting the target substance contained in the matter to be treated into at least either of the hydrophilic fluid component and the hydrophobic fluid component; fractionating the high-pressure fluid mixture into a fraction mainly containing the hydrophilic fluid component and a fraction mainly containing the hydrophobic fluid component; and isolating the target substance from at least one of the fractions.

Description

Specification

Target substance extraction method and target substance extraction apparatus

Technical field

[0001] The present invention relates to an extraction method and an extraction apparatus for extracting a target substance from a solid workpiece.

Background art

[0002] As a method of extracting useful components contained in natural agricultural products, etc. or removing residual agricultural chemical components contained in natural agricultural products, etc. In some cases, these states are simply referred to as “critical states.”) (See, for example, Patent Documents 1 to 3).

[0003] Carbon dioxide exhibits a property as a critical fluid that has both a gas property (diffusibility) and a liquid property (solubility) by adjusting the pressure even at about room temperature. Also, carbon dioxide in the critical state can be easily gasified and removed by adjusting the temperature and pressure. In addition, carbon dioxide is chemically inert and nontoxic. For this reason, carbon dioxide has been suitably used as an extraction solvent for extracting useful components and residual agricultural chemical components from food materials.

[0004] Carbon dioxide in the critical state is relatively high for hydrophobic substances such as essential oils and low molecular weight lipids, etc., but is soluble for hydrophilic substances. Cannot show solubility. In particular, many of the medicinal ingredients contained in natural agricultural products and the like are hardly extracted in high yield because they are bound to hydrophilic substances such as sugar in the natural agricultural products. In addition, for example, carotenoids (xanthophyll, carotene, curcumin, etc.) have a large molecular weight and cannot exhibit sufficient solubility even for hydrophobic substances!

[0005] As a method for solving these problems, Patent Documents 1 to 3 describe that polar adjustment products such as water, lower alcohols such as methanol and ethanol (hereinafter referred to as "entrainers") are used in carbon dioxide in a critical state. )) To improve the polarity of carbon dioxide in the critical state.

[0006] However, according to the method of adding an entrainer to carbon dioxide in a critical state, While the solubility of carbon dioxide in the critical state for water, hydrophilic substances, or useful components with large molecular weight is improved, the solubility in hydrophobic substances is decreased. Therefore, in order to obtain useful components of both hydrophilic and hydrophobic substances from natural agricultural products, a separate extraction process was required.

Patent Document 1: Japanese Patent No. 3010099

Patent Document 2: Japanese Patent No. 2669304

Patent Document 3: Japanese Patent Application Laid-Open No. 200-8659

Disclosure of the invention

[0007] The present invention has been made to solve the above-described conventional problems, and relates to an extraction method for extracting a target substance from an object to be processed such as a natural agricultural product.

[0008] One aspect of the present invention contains a target substance in a high-pressure mixed fluid in which a hydrophilic fluid component and a supercritical or subcritical hydrophobic fluid component are mixed so as to exceed the solubility of each other. A step of contacting a solid object to be processed to extract a target substance contained in the object to be processed into at least one of the hydrophilic fluid component and the hydrophobic fluid component; and from the high-pressure mixed fluid, the hydrophilic fluid A step of separating a fraction containing a component as a main component and a fraction containing the hydrophobic fluid component as a main component, and a step of separating the target substance from at least one of the fractions. This is a target substance extraction method.

Brief Description of Drawings

FIG. 1 is an explanatory diagram illustrating the configuration of a first embodiment of a target substance extraction device of the present invention.

FIG. 2 is an explanatory view illustrating the configuration of a second embodiment of the target substance extraction device of the present invention.

FIG. 3 is an explanatory diagram illustrating the configuration of a third embodiment of the target substance extraction device of the present invention.

FIG. 4 is a diagram showing the relative volatility of coffee ingredients to the components contained in green coffee beans. BEST MODE FOR CARRYING OUT THE INVENTION

[0010] A target substance extraction method according to an embodiment of the present invention includes a high-pressure mixed fluid in which a hydrophilic fluid component and a supercritical or subcritical hydrophobic fluid component are mixed exceeding their solubility. Is brought into contact with a solid workpiece containing the target substance, and the target substance contained in the workpiece is extracted into at least one of a hydrophilic fluid component and a hydrophobic fluid component. A target substance from at least one fraction, a step of separating a fraction containing a hydrophilic fluid component as a main component and a fraction containing a hydrophobic fluid component as a main component from a high-pressure mixed fluid. Separating. Hereinafter, each process is explained in full detail.

[0011] The object to be processed by the extraction method of the present embodiment is not particularly limited as long as it is a solid object to be processed containing the target substance. (Ω represents a hydrophilic target substance contained in the object to be treated. (B) represents a hydrophobic target substance contained in the object to be treated).

Ginseng root; ω-polysaccharide, ginsenoside, (b) pesticides (procymidone, benzenehexachloride (hereinafter sometimes referred to as BHC))

Black ginseng root; (a) Anthocyanin, (b) Pesticide

Bongkan peel; ω nobiletin, (b) pesticide (methidathion)

Maria Thistle: ω Silymarin, (b) Pesticides (malathion, parathion)

Green coffee beans; (a) polyphenols, (b) caffeine

Propolis; ( _a ) amino acids, minerals, organic acids, (b) terpenoids, flavonoids plant seeds; (a) phospholipids, (b) neutral lipids

Glazes; (a) peruflorin (analgesic effect), (b) fat-soluble component (anti-inflammatory effect)

[0012] It is preferable that the workpiece to be processed by this extraction method is pulverized to a predetermined size. By being pulverized in this way, the non-processed product can maintain a large contact area with the hydrophilic fluid component in the high-pressure mixed fluid and the hydrophobic fluid component in the critical state. The particle size of the object to be treated is preferably 20 m or more, more preferably 40 m or more, and even more preferably 50 m or more. If the particle size of the workpiece is smaller than 20 m, the handling property of the workpiece may be deteriorated. Further, the particle size of the object to be treated is preferably 300 m or less, more preferably 500 m or less, more preferably force S, and further preferably 250 m or less. When the particle size of the object to be processed is larger than 500 m, the object to be processed cannot be sufficiently brought into contact with the hydrophilic fluid component and the hydrophobic fluid component in the critical state. Substance extraction may not be sufficient.

[0013] The high-pressure mixed fluid in the present extraction method is obtained by bringing a workpiece into contact with the high-pressure mixed fluid. In order to extract the target substance contained in the object to be processed, it may be formed in any manner as long as it is composed of a hydrophilic fluid component and a critically hydrophobic fluid component. For example, it is formed by mixing a hydrophilic liquid component and a hydrophobic fluid component in a critical state, or a mixed fluid obtained by mixing a hydrophilic gas component and a non-critical hydrophobic fluid component. It may be formed by changing the fluid to a mixed fluid of a hydrophilic liquid component and a critical hydrophobic fluid component under high pressure. Also, a mixed fluid is obtained by mixing a hydrophilic gas component and a critical hydrophobic fluid component, and the resulting mixed fluid is brought to a high pressure to mix the hydrophilic liquid component with the critical hydrophobic fluid component. It may be formed by changing to a fluid. Alternatively, a hydrophilic fluid component and a non-critical hydrophobic fluid component are mixed to obtain a mixed fluid, and the resulting mixed fluid is pressurized to increase the hydrophilic liquid component and the critical hydrophobic fluid component. It may be formed by changing to a mixed fluid. As used herein, “fluid” is a generic term for gases and liquids, or fluids in a supercritical or subcritical state.

[0014] In the present extraction method, the method of contacting the object to be processed with the high-pressure mixed fluid is such that the object to be processed sufficiently contacts with the hydrophilic fluid component constituting the high-pressure mixed fluid and the hydrophobic fluid component in the critical state. If it does, it will not be limited in particular.

[0015] As a specific example of the method of bringing the object to be processed into contact with the high-pressure mixed fluid, for example, the object to be processed is thrown into a pre-formed high-pressure mixed fluid and the object to be processed is immersed in the high-pressure mixed fluid. Or a method in which a hydrophilic fluid component and a hydrophobic fluid component in a critical state are separately supplied to a workpiece simultaneously, or a workpiece is immersed in one fluid component and the other fluid component is immersed in the fluid component. And the like. It is particularly preferable to immerse the object to be treated in a hydrophilic liquid to obtain an immersion liquid, and then mix the immersion liquid with a critical state hydrophobic fluid, or the object to be processed into a critical state hydrophobic fluid. There is a method of dipping to obtain a dipping solution, and then mixing the dipping solution with a hydrophilic liquid.

[0016] Further, in the method of bringing the object to be treated into contact with the high-pressure mixed fluid, both fluid components are separately supplied to the object to be treated separately, or the object to be treated is immersed in one fluid component, Next, in the case of using a method in which the other fluid component is mixed with this, it is preferable that the fluid components are supplied so as to counter flow. Such a supply may, for example, contain one fluid component. It is performed by supplying from one side of the workpiece and supplying the other fluid component from the other side of the workpiece. Thus, the force S can sufficiently mix the fluid components in the high-pressure mixed fluid.

[0017] Further, in the method of bringing the object to be processed into contact with the high-pressure mixed fluid, both fluid components are separately supplied to the object to be processed separately, or the object to be processed is immersed in one fluid component, Next, in the case where the other fluid component is mixed with this, it is preferable that at least one fluid component is supplied in the form of small bubbles. Such supply can be performed, for example, by passing the fluid component through the porous plate or intermittently supplying the fluid component. Thereby, the contact area of a mutual fluid component can be enlarged.

[0018] In the high-pressure mixed fluid in this extraction method, the hydrophilic fluid component is mixed exceeding the solubility of the hydrophobic fluid component in the critical state, and the hydrophobic fluid component in the critical state exceeds the solubility of the hydrophilic fluid component. And mixed. Thus, by mixing each component exceeding the solubility of each other, the high-pressure mixed fluid contains the dissolved hydrophobic fluid component and is dissolved with the phase mainly composed of the hydrophilic fluid component. Thus, the phase containing the hydrophilic fluid component and having the hydrophobic fluid component as the main component is present in a state clearly separated from each other. Then, two fractions consisting of a fraction mainly composed of a hydrophilic fluid component and a fraction mainly composed of a hydrophobic fluid component are formed. In many cases, the fraction containing the hydrophilic fluid component as a main component and the fraction containing the hydrophobic fluid component as a main component each form a layer due to the difference in density between the two. In the following description, a layer having a hydrophilic fluid component as a main component may be referred to as a hydrophilic fluid layer, and a fraction layer having a hydrophobic fluid component as a main component may be referred to as a hydrophobic fluid layer.

[0019] The ratio of the fraction mainly composed of the hydrophilic fluid component and the fraction mainly composed of the hydrophobic fluid component in the high-pressure mixed fluid is not particularly limited as long as the target substance can be extracted from each fraction. . The ratio varies depending not only on the mixing ratio of the hydrophilic fluid component and the hydrophobic fluid component, but also on the characteristics of the hydrophilic fluid component and the hydrophobic fluid component, and the pressure and temperature at which the high-pressure mixed fluid is maintained. .

[0020] For example, when the hydrophilic fluid component is a continuous phase and the hydrophobic fluid component is a dispersed phase, the proportion of the fraction mainly composed of the hydrophilic fluid component is 60 to 99 wt%, and the hydrophobic fluid component is main The proportion of the fraction as the component is preferably from! To 40 wt% The proportion of the fraction that is mainly composed of the hydrophilic fluid component is 70 to 95 wt%, and the hydrophobic fluid component is the main component More preferably, the fraction fraction is composed of 5 to 30 wt%. When the ratio of the fraction containing the hydrophilic fluid component as a main component is less than 60 wt%, the hydrophilic target material cannot be sufficiently extracted into the hydrophilic fluid component among the target materials contained in the object to be treated. In addition, if the ratio of the fraction mainly composed of the hydrophilic fluid component is higher than 99 wt%, the ratio of the fraction mainly composed of the hydrophobic fluid component is inevitably decreased, so that Of the target substances contained, the hydrophobic target substance may not be sufficiently extracted into the hydrophobic fluid component. The continuous phase is a matrix phase that spreads uniformly in any one of a liquid phase, a gas phase, a supercritical state, and a subcritical state, and the dispersed phase is a matrix composed of a continuous phase. It is a domain phase in which the phases are dispersed in a different phase state from the continuous phase.

[0021] When the hydrophobic fluid component is used as the continuous phase and the hydrophilic fluid component is used as the dispersed phase, the proportion of the fraction mainly composed of the hydrophobic fluid component is 60 to 99 wt%. It is preferable that the fraction of the main component is composed of 1 to 40 wt%. The proportion of the main component of the hydrophobic fluid component is 70 to 95 wt%, and the hydrophilic fluid component is the main component. It is more preferable that the fraction of the component is composed of 5 to 30 wt%! /. If the fraction of the main component of the hydrophobic fluid component is less than 60 wt%, it is not possible to sufficiently extract the hydrophobic target material into the hydrophobic fluid component among the target materials contained in the processed material. . In addition, when the proportion of the fraction containing the hydrophobic fluid component as the main component is higher than 99 wt%, the proportion of the fraction containing the hydrophilic fluid component as a main component inevitably decreases. Of the target substances contained, hydrophilic target substances may not be sufficiently extracted into the hydrophilic fluid component.

[0022] The high-pressure mixed fluid in the present extraction method is preferably maintained at IMPa or higher, and more preferably maintained at 5 MPa or higher. If it is lower than IMPa, the critical state of the hydrophobic fluid component may not be maintained in the high-pressure mixed fluid. Further, it is preferably maintained at 100 MPa or less, more preferably 90 MPa or less. 1 Even if it is higher than OOMPa, the solubility of the target substance in the hydrophilic fluid component and / or hydrophobic fluid component does not improve to the left. [0023] The high-pressure mixed fluid in the extraction method of the present embodiment is preferably maintained at 5 ° C or higher, more preferably at 20 ° C or higher. When the temperature is lower than 5 ° C, the extraction speed of the target substance for the treatment force may decrease. Further, the force S is preferably maintained at 100 ° C or lower, and more preferably 90 ° C or lower. When the temperature is higher than 100 ° C, the target substance contained in the material to be processed may cause heat denaturation.

[0024] In the present extraction method, when the object to be treated contains only the hydrophilic target substance, the hydrophilic target substance is mainly obtained by bringing the object to be treated into contact with the high-pressure mixed fluid. Extracted into the hydrophilic fluid component. In addition, when the object to be treated contains only a hydrophobic target substance! /, This hydrophobic target substance is mainly extracted into a hydrophobic fluid component in a critical state. In addition, when the object to be treated contains a hydrophilic target substance and a hydrophobic target substance, the hydrophilic target substance is mainly extracted into the hydrophilic fluid component, and the hydrophobic target substance is mainly in a critical state. Extracted into the hydrophobic fluid component.

[0025] In the present extraction method, there is no particular limitation on the method for separating a fraction containing a hydrophilic fluid component as a main component and a fraction containing a hydrophobic fluid component as a main component from a high-pressure mixed fluid. For example, the remaining lower fraction can be obtained by first extracting the fraction above the interface between the hydrophilic fluid layer and the hydrophobic fluid layer formed in the high-pressure mixed fluid. Also, by first extracting the fraction below the interface, the remaining upper fraction can be obtained.

[0026] The method for separating the target substance from the collected fraction is not particularly limited, but a method for separating the dissolved target substance without heat denaturation is preferred. Specifically, for example, there is a method of separating and separating the gasified fluid component and the target substance by gasifying the separated fluid component under reduced pressure and heating. In addition, the fractionation method in the step of fractionating the fraction containing the hydrophilic component as the main component and the fraction containing the hydrophobic fluid component as the main component from the high-pressure mixed fluid, and the target substance from the fractionated fraction. The separation method in the step of separating may be a method using the same chemical method or physical method.

[0027] It should be noted that the object to be processed which is processed by the present extraction method is preferably processed in a state of being stored in a liquid-permeable container. When the object to be processed is used after being pulverized, the handleability of the object to be processed can be improved by storing the object to be processed in a liquid container.

[0028] The hydrophilic fluid component and the hydrophobic fluid component used in the extraction method have a density of It is preferable to select a combination that increases the difference between the two because the preparative process can be easily performed. Moreover, it is preferable that the hydrophobic fluid component is in a critical state under relatively mild conditions. It is also necessary to consider that this extraction method may be performed on food ingredients.

[0029] Therefore, in the present extraction method, at least one selected from polar solvents such as water, methanol, and ethanol is used as the hydrophilic fluid component, and carbon dioxide, suboxide is used as the hydrophobic fluid component. It is preferable to use at least one selected from nonpolar or weakly polar fluids such as nitrogen and propane. In particular, it is preferable to use water as the hydrophilic fluid component and carbon dioxide as the hydrophobic fluid component. Water is harmless to food ingredients, and carbon dioxide is not toxic or flammable, and can easily reach a critical state (approximately 31 ° C, approximately 7.3 MPa). This is because it is inexpensive as a raw material.

[0030] In this extraction method, when water is used as a hydrophilic fluid component and carbon dioxide is used as a hydrophobic fluid component, high-pressure mixing is performed by mixing these fluid components. It is preferred that the fluid be maintained at 3 MPa or higher. More preferably, the fluid is maintained at 4 MPa or higher. If the high-pressure mixed fluid is held at less than 3 MPa, the object to be processed may freeze in the high-pressure mixed fluid and the desired extraction process may not be possible. The high-pressure mixed fluid is preferably maintained at 10 OMPa or less, more preferably 90 MPa or less.

[0031] In the present extraction method, when ethanol is used as the hydrophilic fluid component and carbon dioxide is used as the hydrophobic fluid component, a high concentration of these fluid components is mixed. The pressure mixed fluid is preferably maintained at 3 MPa or more. It is preferable that the pressure mixed fluid is maintained at 4 MPa or more. If the high-pressure mixed fluid is held at less than 3 MPa, the object to be processed may freeze in the high-pressure mixed fluid and the desired extraction process may not be possible. The high-pressure mixed fluid is preferably maintained at 20 MPa or less, more preferably 15 MPa or less. If the high-pressure mixed fluid is maintained at a pressure exceeding 20 MPa, the solubility of each fluid component increases, and it may not be possible to form a hydrophilic fluid layer and a hydrophobic fluid layer! /. [0032] Although the embodiment of the target substance extraction method of the present embodiment has been described in detail above, the present invention is not limited to the above-described embodiment, and can be implemented in other modes.

[0033] The extraction method of the present embodiment is a fraction in which the hydrophobic fluid component is the main component in the fractionation process for a predetermined time (hereinafter referred to simply as "the fraction of the hydrophobic fluid component"). High-pressure mixing while fractionating only the fraction containing the hydrophilic fluid component as the main component (hereinafter sometimes simply referred to as “the fraction of the hydrophilic fluid component”) By newly supplying the hydrophilic fluid component into the fluid, the hydrophilic fluid component in the high-pressure mixed fluid is replaced and the target substance is extracted into the hydrophilic fluid component and the fraction of the hydrophilic fluid component is fractionated. Finally, the fraction of the hydrophobic fluid component may be fractionated. Alternatively, only the fraction of the hydrophobic fluid component in the critical state without fractionating the fraction of the hydrophilic fluid component is fractionated, and the hydrophobic fluid component in the critical state is newly supplied into the high-pressure mixed fluid. Therefore, after replacing the hydrophobic fluid component in the high-pressure mixed fluid, extraction of the target substance into the hydrophobic fluid component and fractionation of the hydrophobic fluid component are performed, and finally the hydrophilic fluid The fraction of the components may be collected and performed. In addition, the hydrophilic fluid component and the critical hydrophobic fluid component are newly added to the high-pressure mixed fluid while the hydrophilic fluid component fraction and the critical hydrophobic fluid component fraction are fractionated in the fractionation process. Supply to the high-pressure mixed fluid, while exchanging the hydrophilic fluid component and the hydrophobic fluid component in the high-pressure mixed fluid, extraction of the target substance into the hydrophilic fluid component, fractionation of the hydrophilic fluid component, and hydrophobicity Extraction of the target substance into the ionic fluid component and fractionation of the hydrophobic fluid component may be performed. This prevents the concentration of the target substance in the hydrophilic fluid component and the hydrophobic fluid component constituting the high-pressure mixed fluid from being increased, so that the ability to extract the target substance contained in the object to be processed into the fluid component is improved. The power S can be prevented from falling.

[0034] Alternatively, the extraction method of the present embodiment may be performed by re-feeding at least one of the fractions of the separated fluid component into the high-pressure mixed fluid as it is without being sent to the separation step. . For example, there is a case where the extractability of the hydrophilic fluid component may be reduced by re-feeding at least a part of the fraction of the separated hydrophilic fluid component into the high-pressure mixed fluid as it is without sending it to the separation process. The target substance concentration in the fraction of hydrophilic fluid components can be increased. Further, the hydrophilic fluid in the extraction method of the present embodiment The amount of ingredients used can be reduced. Furthermore, it is possible to recognize the critical amount of the hydrophobic fluid component that dissolves in the hydrophilic fluid component and disappears from the high-pressure mixed fluid as the fraction of the hydrophilic fluid component is fractionated.

[0035] The extraction method of the present embodiment may be any one that treats an object to be processed in a high-pressure mixed fluid obtained by mixing a hydrophilic fluid component and a hydrophobic fluid component. Therefore, depending on the purpose of extraction, a modifier for modifying the mixed fluid may be added to the high-pressure mixed fluid.

[0036] Next, a target substance extraction apparatus that can suitably perform the target substance extraction method of the present embodiment will be described.

FIG. 1 is an explanatory view illustrating the configuration of the first embodiment of the target substance extraction device according to the present invention. FIG. 2 is an explanatory view illustrating the configuration of the second embodiment of the target substance extraction device according to the present invention. FIG. 3 is an explanatory view illustrating the configuration of the third embodiment of the target substance extraction device according to the present invention.

According to FIG. 1, the target substance extraction apparatus 10a according to the first embodiment includes an extraction tank 12 that contains an object to be processed and extracts the target substance from the object to be processed, and a hydrophilic substance that stores a hydrophilic fluid component. Hydrophilic fluid component storage tank 14, a hydrophobic fluid component storage tank 16 for storing a hydrophobic fluid component, and a hydrophilic fluid having one end connected to the hydrophilic fluid component storage tank 14 and the other end connected to the extraction tank 12. Hydrophobic fluid component supply path 20 having one end connected to the hydrophobic fluid component storage tank 16 and one end connected to the extraction tank 12 and one end connected to the extraction tank 12 and the hydrophilic flow Hydrophilic fluid fractionation path 22 for fractionating fractions mainly composed of body components, and hydrophobic fluid fraction for fractionating fractions mainly composed of hydrophobic fluid components with one end connected to extraction tank 12 The intake path 24, the first separator 26 for separating the target substance from the fraction containing the separated hydrophilic fluid component as a main component, and the separation And a second separator 28 for separating the target substance.

[0039] Hereinafter, a process for extracting a target substance from an object to be processed will be described with reference to FIG. The combination of the hydrophilic fluid component and the hydrophobic fluid component used here forms at least one interface in the extraction tank 12, and the density of the hydrophilic fluid component is the same as that of the hydrophobic fluid component. The one larger than the density is selected. Therefore, in the extraction tank 12 A hydrophobic fluid layer is formed in the upper part, and a hydrophilic fluid layer is formed in the lower part.

First, an object to be processed stored in a liquid-permeable container (not shown) is stored in the extraction tank 12. Next, the hydrophilic fluid component is supplied from the hydrophilic fluid component supply path 18 into the extraction tank 12, and the hydrophobic fluid component is supplied from the hydrophobic fluid component supply path 20. Here, the hydrophobic fluid component supplied from the hydrophobic fluid component supply channel 20 is in a critical state at least in the extraction tank 12. In addition, the hydrophilic fluid component supplied from the hydrophilic fluid component supply path 18 is a hydrophilic liquid at least in the extraction tank 12.

[0041] The method for bringing the hydrophobic fluid component into the critical state in the extraction tank 12 is not particularly limited. Specific examples thereof include, for example, supplying a subcritical hydrophobic fluid component into the extraction tank 12 to pressurize the extraction tank 12, and then using the temperature adjusting means 30 provided in the extraction tank 12 to extract the extraction tank. 12 by adjusting the temperature in 12 and changing it to a critical state, or a non-critical hydrophobic fluid component supplied from the hydrophobic fluid component storage tank 16 and a booster pump provided in the hydrophobic fluid component supply path 20 For example, a method of supplying the liquid into the extraction tank 12 as a critical state in advance by the heater 32 and the heater 34 may be used. As a more preferable method, the temperature in the extraction tank 12 is adjusted in advance by the temperature adjusting means 30 provided in the extraction tank 12, and a non-critical hydrophobic fluid component is supplied into the extraction tank 12 for extraction. An example is a method in which after the inside of the tank 12 is pressurized, the hydrophobic fluid component is supplied into the extraction tank 12 as a critical state.

[0042] If the hydrophilic fluid component supplied from the hydrophilic fluid component supply path 18 becomes a hydrophilic liquid in the extraction tank 12, the form of the hydrophilic fluid component is not particularly limited. It may be in a (gas) state. In order to supply the hydrophilic fluid component into the extraction tank 12 whose internal pressure has been increased, it is preferable that the hydrophilic fluid component supply path 18 is provided with a booster pump 36. In addition, in order to maintain the hydrophobic fluid component contained in the extraction tank 12 in a critical state, when the temperature in the extraction tank 12 is adjusted to a predetermined temperature, the temperature in the extraction tank 12 is adjusted. In order not to fluctuate, it is preferable to provide a heater (not shown) in the hydrophilic fluid component supply path 18 so that the hydrophilic fluid component is heated to a predetermined temperature in advance and then supplied into the extraction tank 12.

[0043] In the target substance extraction apparatus 10a, the supply method of the hydrophilic fluid component into the extraction tank 12 and the supply method of the hydrophobic fluid component in the critical state include the target substance contained in the object to be processed in a high-pressure mixed flow. Extraction force to at least one of the fraction of hydrophilic fluid component and the fraction of hydrophobic fluid component in the body S is not particularly limited as long as it can be extracted. For example, even if a hydrophilic fluid component and a hydrophobic fluid component in a critical state are simultaneously supplied into the extraction tank 12, one fluid component is supplied into the extraction tank 12 in advance and the immersion liquid for the object to be processed is supplied. The other fluid component may be supplied here.

[0044] The two fluid components supplied into the extraction tank 12 are preferably mixed and contacted sufficiently. By sufficiently mixing and contacting the two fluid components in this way, processing in the extraction process can be accelerated.

[0045] In order to sufficiently mix and contact the two fluid components, the hydrophilic fluid component supply path 18 is connected to the upper part of the extraction tank 12, and the hydrophobic fluid component supply path 20 is connected to the lower part of the extraction tank 12. It is preferable to connect. This allows the two fluid components to contact in countercurrent.

[0046] In the extraction tank 12, the contact area of the two fluid components is preferably large. In order to increase the contact area between the two fluid components, for example, a porous plate 38 may be provided in the extraction tank 12 so that at least one of the fluid components passes through the porous plate 38. Thereby, the fluid component can be dispersed in the form of small bubbles.

[0047] The fraction of the hydrophilic fluid component and the fraction of the hydrophobic fluid component from the extraction tank 12 are separated by connecting the hydrophilic fluid fractionation path 22 to the lower part of the extraction tank 12, What is necessary is just to comprise the intake path 24 by connecting to the upper part of the extraction tank 12. Thereby, for example, a fraction of the hydrophilic fluid component is first extracted from the hydrophilic fluid fractionation path 22 connected to the lower part of the extraction tank 12, and then the critical state hydrophobicity left in the extraction tank 12 is extracted. By obtaining fluid component fractions, each fraction can be fractionated. Alternatively, the fraction of the hydrophobic fluid component in the critical state is discharged from the hydrophobic fluid separation path 24 connected to the upper part of the extraction tank 12, and the hydrophilic fluid is discharged from the lower part of the extraction tank 12 by the following! / The fractions of the hydrophilic fluid component can be extracted through the sorting channel 22 to separate each fraction.

[0048] The method of separating the target substance from the fraction collected is not particularly limited! / However, after gasifying the hydrophobic fluid component in the critical state, the gasified hydrophobic fluid component Between the hydrophobic fluid separation path 24 and the second separator 28. A pressure regulating valve 40 and a heater 42 are preferably provided.

[0049] Examples of the first separator 26 include an evaporator. If the first separator 26 also separates the hydrophobic fluid component in the critical state dissolved in the fraction of the hydrophilic fluid component together with the hydrophilic fluid fractionation path 22 It is preferable that a pressure regulating valve and a heater are provided between the first separator 26 (not shown).

As described above, the force explaining the extraction process of the present embodiment with reference to FIG. 1. The target substance extraction device of the present invention is not limited to the above-described embodiment.

[0051] The target substance extraction device 10a is a condenser for collecting and circulating the hydrophobic fluid component separated by the first separator 26 and the second separator 28 to the hydrophobic fluid component storage tank 16. Four

4 may be provided.

[0052] The target substance extraction apparatus 10a is configured to extract the target substance contained in the object to be processed into each fluid layer while replacing the hydrophilic fluid component and / or the hydrophobic fluid component in the extraction tank 12. When performing separation of each fluid layer, for example, to control the supply amount of each fluid component from the supply passages 18 and 20 and the separation amount from the separation passages 22 and 24, for example, a hydrophilic fluid It is preferable to provide a liquid level gauge 46 (for example, a differential pressure transmitter) for measuring the liquid level in the extraction tank 12

Yes

[0053] Further, as another aspect of the target substance extraction device, as in the target substance extraction device 10b shown in FIG. 2, at least a part of the fraction of the hydrophilic fluid component extracted from the lower part of the extraction tank 12 is used. A high-pressure circulation pump 48 may be provided, which is supplied again to the upper part of the extraction tank 12 without being sent to the first separator 26. Note that aspects other than the above in FIG. 2 are the same as those in FIG.

[0054] As another aspect of the target substance extraction device, a target substance extraction device 10c shown in Fig. 3 includes a high-pressure mixed fluid discharge path 50 connected to the upper part of the extraction tank 12, and a high-pressure mixed discharge. The mixed high-pressure fluid discharged from the outlet 50 is temporarily stored into the fraction of the hydrophilic fluid component and the fraction of the hydrophobic fluid component in the critical state (i.e., the hydrophilic fluid layer and the hydrophobic fluid layer). ) It may be configured to include a high-pressure separator 52 for separation, and a hydrophobic fluid separation passage 24 connected to an upper portion of the high-pressure separator 52 to fractionate a fraction of the hydrophobic fluid component! / ,. As a result, even if the high-pressure mixed fluid is discharged from the extraction tank 12, the hydrophilic fluid flow into the hydrophobic fluid separation path 24. Since the body component fraction can be prevented from flowing in, it is no longer necessary to maintain the interface between the hydrophilic fluid layer and the hydrophobic fluid layer in the extraction tank 12 at a constant height, The amount of objects to be processed can be increased.

[0055] If the hydrophilic target substance is extracted from the fraction of the hydrophilic fluid component separated by the high-pressure separator 52, the fraction of the hydrophilic fluid component is separated from the high-pressure separator 52. A hydrophilic fluid separation channel (not shown! / ヽ) is provided to supply to the first separator 26 and separate the hydrophilic target substance from the fraction of the hydrophilic fluid component in the high pressure separator 52 It is preferable to do.

[0056] The other aspects of FIG. 3 are the same as those in FIG. 1 or FIG.

[0057] The target substance extraction devices 10a to 10c shown in FIGS. 1 to 3 supply the fluid component having the higher density and the upper force of the extraction tank by the combination of the hydrophilic fluid component and the hydrophobic fluid component. (And discharged from the lower part), and can be used with appropriate design changes so that the fluid component having the lower density is supplied from the lower part of the extraction tank (and discharged from the upper part).

[0058] In addition, the present invention can be implemented in a mode in which various improvements, modifications, and variations are added based on the knowledge of those skilled in the art without departing from the spirit of the present invention.

Example

[0059] Hereinafter, the present invention will be described more specifically with reference to examples. The present invention is not limited to these examples.

Experimental Example 1 (Relative Volatility of Useful Components in Green Coffee Beans relative to Coffee Oil) 100 g of green coffee beans as an object to be treated is placed in a 300 ml capacity extraction tank, where a predetermined amount of hydrophilic fluid component is contained. (5g, 10g, 30g, 50g, 70g, 90g) water was removed, the inside of the brewing tank was sealed, and then the inside of the extraction tank was heated to 60 ° C. Next, carbon dioxide was filled in the extraction tank, and the inside of the extraction tank was adjusted to 60 ° C and 30 MPa.

[0061] Next, while maintaining the inside of the extraction tank at 60 ° C and 30 MPa, supercritical carbon dioxide is supplied from the lower part of the extraction tank (flow rate of about 10 g / min) and from the upper part of the extraction tank. By discharging the fraction of the critical carbon dioxide layer, supercritical carbon dioxide was circulated in the extraction tank for 3 hours. [0062] The fraction of the supercritical carbon dioxide layer discharged from the extraction tank was decompressed, and the supercritical carbon dioxide was gasified and removed to obtain a liquid extract.

[0063] The obtained liquid extract was analyzed by high performance liquid chromatography (using ODS column manufactured by Shimadzu Corporation), and caffeine and polyphenols contained in the extract were quantified, and the remaining liquid extract was quantified. The extract was coffee oil. The amount of caffeine and the amount of polyphenol relative to the amount of coffee oil were determined, and the relative volatility relative to coffee oil was determined. Figure 4 shows the results.

[0064] From the results in Fig. 4, it was found that the relative volatility of caffeine to coffee oil was selectively improved by increasing the amount of water added to green coffee beans. In addition, the volatility of polyphenols with respect to coffee oil was found to be almost constant even when the amount of water added increased. From this result, it was found that the hydrophobic fluid component and the hydrophilic fluid component coexist, and that only the supercritical carbon dioxide that is the hydrophobic fluid component (that is, the amount of fresh coffee beans added to Owt%). in the case of extraction, the extraction behavior differs greatly, it forces ^s Wakakaru particularly improved the extraction efficiency of the target component when the hydrophilic fluid component is a predetermined amount or more.

[0065] Experimental Example 2

Place 100g of green coffee beans as an object to be processed in a basket with a capacity of 300ml, supply 200g of water as a hydrophilic fluid component, and immerse the green coffee beans in water. After sealing, the inside of the extraction tank was heated to 60 ° C. Subsequently, carbon dioxide was supplied into the extraction tank and pressurized, and the inside of the extraction tank was adjusted to 60 ° C and 30 MPa.

[0066] Next, with the inside of the extraction tank maintained at 60 ° C and 30 MPa, water was supplied from the upper part of the extraction tank (flow rate 2.5 g / min), and from the lower part of the extraction tank to the extraction tank. While supplying supercritical carbon dioxide (flow rate 10g / min) while dispersing in small bubbles with the porous plate provided, the water layer fraction is discharged from the lower part of the extraction tank and from the upper part of the extraction tank. The fractions of the supercritical carbon dioxide layer were discharged, and each fraction was fractionated while water and supercritical carbon dioxide were passed through the extraction tank for 3 hours.

[0067] In this case, the water layer was composed of about 94.5 wt% water and about 5.5 wt% supercritical carbon dioxide. The supercritical carbon dioxide layer is about 0.3 wt% water and about 99.7 wt%. Consists of supercritical carbon dioxide!

[0068] The obtained aqueous layer fraction was heated under reduced pressure to distill off water, thereby obtaining a liquid extract. The obtained extract was analyzed by high performance liquid chromatography (using ODS column manufactured by Shimadzu Corporation).

Caffeine and polyphenols contained in the extract were quantified, and the remaining liquid extract was used as coffee oil. The results are shown in Table 1.

[0069] [Table 1]

[0070] From the results in Table 1, it was found that coffee oil and polyphenols could be extracted at high concentrations (high yield) while selectively removing caffeine from the aqueous layer fraction.

[0071] Further, when the result of Experimental Example 1 is compared with the result of Experimental Example 2, the following can be understood. In other words, in Experimental Example 1, a very small amount of polyphenols was extracted by a hydrophobic fluid layer mainly composed of carbon dioxide, and caffeine was extracted in several to 50 times the amount of polyphenols. In Experimental Example 2, a very small amount of caffeine was extracted by a hydrophilic fluid layer mainly composed of water, and polyphenols were extracted more than 3800 times that of caffeine. From these results, it is shown that according to the above method, the substance extracted by the hydrophobic fluid and the substance extracted by the hydrophilic fluid can be selectively extracted at a high concentration.

[0072] Experimental Example 3

In Experimental Example 2, instead of green coffee beans, water and supercritical carbon dioxide were obtained in the same manner as in Experimental Example 2, except that 100 g of ginseng root having a particle size distribution of 50 to 250 111 was placed in the extraction tank. The carrot root was treated with a high-pressure mixed fluid.

[0073] Thereafter, the fraction of the aqueous layer and the fraction of the supercritical carbon dioxide layer are separated, heated under reduced pressure to remove water or carbon dioxide, and extracted into the fraction of the aqueous layer. Liquid extract and liquid extract extracted in the fraction of the supercritical carbon dioxide layer were obtained. Derived from the fraction of the water layer The extract was analyzed by high performance liquid chromatography (using an ODS column manufactured by Shimadzu Corporation), and ginsenoside was quantified. The remaining liquid extract was used as a polysaccharide. In addition, the extract derived from the fraction of the supercritical carbon dioxide layer was analyzed by gas chromatography (DB1701 column manufactured by GL Sciences Inc.) to quantify pesticides (prosimidone, BHC). The results are shown in Table 2.

[Table 2]

[0075] From the results in Table 2, it was found that ginsenosides and polysaccharides as saponins could be extracted at a high concentration (high yield) while selectively removing pesticides from the aqueous fraction.

[0076] The present invention described above can be summarized as follows.

[0077] One aspect of the present invention contains a target substance in a high-pressure mixed fluid in which a hydrophilic fluid component and a supercritical or subcritical hydrophobic fluid component are mixed in excess of the solubility of each other. A step of contacting a solid object to be processed to extract a target substance contained in the object to be processed into at least one of the hydrophilic fluid component and the hydrophobic fluid component; and from the high-pressure mixed fluid, the hydrophilic fluid A step of separating a fraction containing a component as a main component and a fraction containing the hydrophobic fluid component as a main component, and a step of separating the target substance from at least one of the fractions. This is a target substance extraction method. According to such a method, when a target substance is extracted from a processed object such as a natural agricultural product, a hydrophilic target substance and a hydrophobic target substance contained in the processed object are extracted by a single extraction operation. It is possible to extract with high extraction efficiency while separating separately. In addition, since the extraction operation can be performed with a high-pressure mixed fluid in one extraction tank, the apparatus can be simplified. Furthermore, the time required for the extraction process can be shortened. As described above, according to the extraction method of the present invention, the hydrophilic target substance can be separated from the object to be processed without requiring a separate extraction step. The aqueous target substance can be extracted separately and with high purity.

[0078] In the target substance extraction method, the hydrophilic target substance is extracted from the fraction containing the hydrophilic fluid component as a main component, and the hydrophobic target substance is extracted from the fraction containing the hydrophobic fluid component as a main component. Extracting can be performed by separately separating the hydrophilic target substance and the hydrophobic target substance contained in the object to be processed by a single extraction operation.

[0079] Further, among the hydrophilic fluid component and the hydrophobic fluid component, V, the treatment object is immersed in one of the fluid components to obtain an immersion fluid, and the other fluid component is added to the immersion fluid. It is preferable that the object to be treated is brought into contact with the high-pressure mixed fluid by mixing in order to simplify the process.

[0080] Further, among the hydrophilic fluid component and the hydrophobic fluid component, V, one of the fluid components, and the other fluid component are supplied in the form of small bubbles to form the high-pressure mixed fluid. However, it is preferable because it can increase the contact area of the fluid components.

[0081] In addition, it is preferable that the hydrophilic fluid component and the hydrophobic fluid component are supplied in countercurrent to form the high-pressure mixed fluid from the viewpoint that the fluid components can be sufficiently mixed and contacted.

[0082] Further, at least one selected from water, methanol, and ethanol is used as the hydrophilic fluid component, and at least one selected from carbon dioxide, nitrous oxide, and propane is used as the hydrophobic fluid component. It is preferable to use it because the difference in density between the hydrophilic fluid component and the hydrophobic fluid component is large, so that the separation is easy, and the hydrophobic fluid component becomes a critical state under relatively mild conditions.

[0083] Further, one aspect of the present invention contains a solid object to be processed containing a target substance, a hydrophilic fluid component, and a supercritical or subcritical hydrophobic fluid component, An extraction tank for extracting a target substance from an object to be treated; a hydrophilic fluid fractionation path for fractionating a fraction containing the hydrophilic fluid component as a main component from the extraction tank; and the extraction tank, A hydrophobic fluid fractionation path for fractionating a fraction mainly composed of a hydrophobic fluid component, and a first separator for separating the target substance from the fraction fractionated in the hydrophilic fluid fractionation path And a second separator that separates the target substance from the fraction fractionated in the hydrophobic fluid fractionation channel. [0084] Further, according to one aspect of the present invention, a solid object to be processed containing a target substance, a hydrophilic fluid component, and a hydrophobic fluid component in a supercritical state or a subcritical state are accommodated. An extraction tank for extracting a target substance from an object to be treated; a hydrophilic fluid fractionation path for fractionating a fraction containing the hydrophilic fluid component as a main component from the extraction tank; and an upper part of the extraction tank A fluid discharge path connected to the extraction tank, a high-pressure separator for storing the fluid discharged from the extraction tank, and a fraction mainly composed of the hydrophobic fluid component connected to an upper portion of the high-pressure separator. A first separator for separating the target substance from a fraction separated in the hydrophilic fluid fractionation path, and a fractionation from the hydrophobic fluid fractionation path. And a second separator for separating the target substance from the fraction obtained. Device.

[0085] According to these apparatuses, when a target substance is extracted from an object to be processed such as a natural agricultural product, various targets contained in the object to be processed can be obtained by a single extraction operation without requiring a separate extraction step. The substance can be extracted with high purity while separately separating the hydrophilic target substance and the hydrophobic target substance.

[0086] Also, a hydrophilic fluid component storage tank that stores the hydrophilic fluid component, a hydrophilic fluid component supply path that supplies the hydrophilic fluid component from the hydrophilic fluid component storage tank to the extraction tank, and Hydrophobic fluid component storage tank for storing a hydrophobic fluid component, and a hydrophobic fluid component supply path for supplying the hydrophobic fluid component from the hydrophobic fluid component storage tank to the extraction tank, and supplying the hydrophilic fluid component The fluid component and the hydrophobic fluid component supply channel are prepared so that the hydrophilic fluid component and the hydrophobic fluid component are supplied countercurrently into the extraction tank. It is preferable from the point which can be made to contact.

[0087] Further, the hydrophobic fluid component supply path supplies the hydrophilic fluid component from the upper part to the lower part of the extraction tank, and the hydrophobic fluid component supply path extracts the hydrophobic fluid component from the extraction tank. The hydrophilic fluid fractionation path is connected so as to fractionate a fraction mainly composed of the hydrophilic fluid component from the lower part of the extraction tank so as to be supplied from the lower part to the upper part. Can be sufficiently mixed and brought into contact with each fluid component, and is preferable from the viewpoint of easy fractionation.

[0088] Further, the hydrophobic fluid separation path is formed from the upper part of the extraction tank with the hydrophobic fluid component. It is preferable to be connected so as to fractionate fractions containing as the main component from the viewpoint of easy fractionation.

[0089] In addition, the extraction tank includes a porous plate that disperses at least one of the hydrophilic fluid component and the hydrophobic fluid component in the form of small bubbles inside the extraction tank. It is preferable that the contact area between the fluid components can be increased by supplying the foam in the form of foam.

[0090] In addition, it is preferable that a circulation device for supplying at least a part of the fraction collected to the extraction tank is provided because the concentration of the target substance in the fraction of the hydrophilic fluid component can be increased.

[0091] The second separator further includes a condenser that condenses the hydrophobic fluid component separated from the target substance and reduces the hydrophobic fluid component to the hydrophobic fluid component storage tank. Is preferable from the viewpoint that the hydrophobic fluid component can be recycled.

Industrial applicability

[0092] The target substance extraction method of the present invention is suitably used for selective extraction of a target substance from an object to be processed.

Claims

The scope of the claims

[1] A high-pressure mixed fluid, in which a hydrophilic fluid component and a supercritical or subcritical hydrophobic fluid component are mixed in excess of each other's solubility, is used as a solid workpiece containing the target substance. Extracting the target substance contained in the object to be treated into at least one of the hydrophilic fluid component and the hydrophobic fluid component;

Separating a fraction mainly comprising the hydrophilic fluid component and a fraction mainly comprising the hydrophobic fluid component from the high-pressure mixed fluid;

Separating the target substance from at least one of the fractions;

A method for extracting a target substance.

[2] The target according to claim 1, wherein a hydrophilic target substance is extracted from a fraction containing the hydrophilic fluid component as a main component, and a hydrophobic target substance is extracted from a fraction containing the hydrophobic fluid component as a main component. Substance extraction method.

[3] An immersion liquid is obtained by immersing the object to be treated in one of the hydrophilic fluid component and the hydrophobic fluid component, and the other fluid component is mixed with the immersion liquid. 2. The target substance extraction method according to claim 1, wherein the object to be processed is brought into contact with the high-pressure mixed fluid.

[4] The high-pressure mixed fluid according to claim 1, wherein the high-pressure mixed fluid is formed by supplying one of the hydrophilic fluid component and the hydrophobic fluid component to one of the fluid components in the form of small bubbles. Target substance extraction method.

5. The target substance extraction method according to claim 1, wherein the hydrophilic fluid component and the hydrophobic fluid component are supplied in countercurrent to form the high-pressure mixed fluid.

[6] At least one selected from water, methanol, and ethanol is used as the hydrophilic fluid component, and at least one selected from carbon dioxide, nitrous oxide, and propane is used as the hydrophobic fluid component. The target substance extraction method according to claim 1.

[7] To contain a solid object to be processed containing a target substance, a hydrophilic fluid component, and a supercritical or subcritical hydrophobic fluid component and extract the target substance from the object to be processed An extraction tank of

A hydrophilic flow for separating a fraction mainly composed of the hydrophilic fluid component from the extraction tank. Body parting route,

A hydrophobic fluid fractionation path for fractionating a fraction mainly composed of the hydrophobic fluid component from the extraction tank;

A first separator for separating the target substance from a fraction fractionated in the hydrophilic fluid fractionation path;

A second separator for separating the target substance from a fraction fractionated in the hydrophobic fluid fractionation path;

A target substance extraction apparatus comprising:

[8] In order to extract a target substance from the target object, containing a solid target object containing the target substance, a hydrophilic fluid component, and a supercritical or subcritical hydrophobic fluid component An extraction tank of

A hydrophilic fluid fractionation path for fractionating a fraction mainly comprising the hydrophilic fluid component from the extraction tank;

A fluid discharge path connected to an upper portion of the extraction tank;

A high-pressure separator for storing the fluid discharged from the extraction tank;

A hydrophobic fluid fractionation path connected to an upper portion of the high-pressure separator and fractionating a fraction mainly composed of the hydrophobic fluid component;

A target substance extraction apparatus comprising: a second separator that separates the target substance from a fraction collected from the hydrophobic fluid sorting path.

[9] A hydrophilic fluid component storage tank for storing the hydrophilic fluid component;

A hydrophilic fluid component supply path for supplying the hydrophilic fluid component from the hydrophilic fluid component storage tank to the extraction tank;

A hydrophobic fluid component reservoir for storing the hydrophobic fluid component;

A hydrophobic fluid component supply path for supplying the hydrophobic fluid component from the hydrophobic fluid component storage tank to the extraction tank;

The hydrophilic fluid component supply channel and the hydrophobic fluid component supply channel are hydrophilic. The target substance extraction device according to claim 7 or 8, wherein the fluid component and the hydrophobic fluid component are provided so as to be supplied in a counter-current manner into the extraction tank.

[10] The hydrophilic fluid component supply path supplies the hydrophilic fluid component from the upper part to the lower part of the extraction tank.

The hydrophobic fluid component supply path supplies the hydrophobic fluid component from the lower part to the upper part of the extraction tank.

10. The target substance extraction according to claim 9, wherein the hydrophilic fluid fractionation path is connected so as to fractionate a fraction containing the hydrophilic fluid component as a main component from a lower part of the extraction tank. Out device.

[11] The hydrophobic fluid sorting channel according to claim 7, wherein the hydrophobic fluid sorting channel is connected so as to sort a fraction mainly composed of the hydrophobic fluid component from an upper part of the extraction tank. Target substance extraction device.

[12] The target according to claim 7 or 8, wherein the extraction tank includes a porous plate that disperses at least one of the hydrophilic fluid component and the hydrophobic fluid component in a small bubble shape inside the extraction tank. Substance extraction device.

[13] The target substance extraction device according to [7] or [8], further comprising a circulation device that supplies at least a part of the fractionated fraction to the extraction tank.

14. A condenser for condensing the hydrophobic fluid component separated from the target substance in the second separator and reducing the hydrophobic fluid component to the hydrophobic fluid component storage tank. Or the target substance extraction apparatus according to 8.