WO2008025006A3 - Spectral unmixing for in-vivo imaging - Google Patents

Spectral unmixing for in-vivo imaging Download PDF

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Publication number
WO2008025006A3
WO2008025006A3 PCT/US2007/076813 US2007076813W WO2008025006A3 WO 2008025006 A3 WO2008025006 A3 WO 2008025006A3 US 2007076813 W US2007076813 W US 2007076813W WO 2008025006 A3 WO2008025006 A3 WO 2008025006A3
Authority
WO
WIPO (PCT)
Prior art keywords
spectral unmixing
light sources
spectrum
vivo imaging
spectra
Prior art date
Application number
PCT/US2007/076813
Other languages
French (fr)
Other versions
WO2008025006A2 (en
Inventor
Heng Xu
Bradley W Rice
Original Assignee
Xenogen Corp
Heng Xu
Bradley W Rice
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xenogen Corp, Heng Xu, Bradley W Rice filed Critical Xenogen Corp
Priority to EP07841365A priority Critical patent/EP2068714A2/en
Publication of WO2008025006A2 publication Critical patent/WO2008025006A2/en
Publication of WO2008025006A3 publication Critical patent/WO2008025006A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0073—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by tomography, i.e. reconstruction of 3D images from 2D projections
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B2503/00—Evaluating a particular growth phase or type of persons or animals
    • A61B2503/40—Animals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0062—Arrangements for scanning
    • A61B5/0064—Body surface scanning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/4795—Scattering, i.e. diffuse reflection spatially resolved investigating of object in scattering medium

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Surgery (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Radiology & Medical Imaging (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Described herein are systems and methods for spectral unmixing of in vivo light data. The spectral unmixing separates image data according to spectra from multiple internal light sources in an effort to isolate one or more spectrum of interest. The spectral unmixing obtains images with a combination of different and known excitation and emission limits. The spectral unmixing then uses an iterative solution process to separate spectra for the multiple fluorescent light sources, and provides a spectrum and/or a spatial distribution map for at least one of the internal light sources.
PCT/US2007/076813 2006-08-24 2007-08-24 Spectral unmixing for in-vivo imaging WO2008025006A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07841365A EP2068714A2 (en) 2006-08-24 2007-08-24 Spectral unmixing for in-vivo imaging

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84024706P 2006-08-24 2006-08-24
US60/840,247 2006-08-24

Publications (2)

Publication Number Publication Date
WO2008025006A2 WO2008025006A2 (en) 2008-02-28
WO2008025006A3 true WO2008025006A3 (en) 2008-10-23

Family

ID=39107725

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2007/076781 WO2008024986A2 (en) 2006-08-24 2007-08-24 Apparatus and methods for determining optical tissue properties
PCT/US2007/076813 WO2008025006A2 (en) 2006-08-24 2007-08-24 Spectral unmixing for in-vivo imaging

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2007/076781 WO2008024986A2 (en) 2006-08-24 2007-08-24 Apparatus and methods for determining optical tissue properties

Country Status (2)

Country Link
EP (1) EP2068714A2 (en)
WO (2) WO2008024986A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2920874B1 (en) 2007-09-10 2010-08-20 Biospace Lab LUMINESCENCE IMAGING INSTALLATION AND METHOD
FR2969497B1 (en) 2010-12-27 2013-06-28 Ceva Sante Animale LUMINESCENT COMPOSITION AS A BIOMARKER IN AN AVIAN EGG, CORRESPONDING DEVICE AND METHOD.
EP2518474A1 (en) 2011-04-27 2012-10-31 Well Dynamics Applications S.r.l. WDA Method for the determination of analytes in the hair
US9314218B2 (en) 2011-06-20 2016-04-19 Caliper Life Sciences, Inc. Integrated microtomography and optical imaging systems
CN107184181A (en) * 2017-05-15 2017-09-22 清华大学 The processing method and system of Dynamic Fluorescence molecular tomographic
EP3517934A1 (en) 2018-01-24 2019-07-31 I Love My Body Research S.r.l. Method for the determination of analytes in the hair

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060146346A1 (en) * 2004-12-06 2006-07-06 Hoyt Clifford C Systems and methods for in-vivo optical imaging and measurement

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1402243B1 (en) * 2001-05-17 2006-08-16 Xenogen Corporation Method and apparatus for determining target depth, brightness and size within a body region

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060146346A1 (en) * 2004-12-06 2006-07-06 Hoyt Clifford C Systems and methods for in-vivo optical imaging and measurement
US20070016078A1 (en) * 2004-12-06 2007-01-18 Hoyt Clifford C Systems and methods for in-vivo optical imaging and measurement

Also Published As

Publication number Publication date
WO2008024986A2 (en) 2008-02-28
EP2068714A2 (en) 2009-06-17
WO2008024986A3 (en) 2008-04-10
WO2008025006A2 (en) 2008-02-28

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