WO2008024343A3 - Micro rna arrays and methods of using the same - Google Patents

Micro rna arrays and methods of using the same Download PDF

Info

Publication number
WO2008024343A3
WO2008024343A3 PCT/US2007/018478 US2007018478W WO2008024343A3 WO 2008024343 A3 WO2008024343 A3 WO 2008024343A3 US 2007018478 W US2007018478 W US 2007018478W WO 2008024343 A3 WO2008024343 A3 WO 2008024343A3
Authority
WO
WIPO (PCT)
Prior art keywords
mirna
methods
nucleic acid
polynucleic
acid sequence
Prior art date
Application number
PCT/US2007/018478
Other languages
French (fr)
Other versions
WO2008024343A2 (en
Inventor
Dirk Dittmer
Wolfgang Vahrson
Original Assignee
Univ North Carolina
Dirk Dittmer
Wolfgang Vahrson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina, Dirk Dittmer, Wolfgang Vahrson filed Critical Univ North Carolina
Publication of WO2008024343A2 publication Critical patent/WO2008024343A2/en
Publication of WO2008024343A3 publication Critical patent/WO2008024343A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Isolated polynucleic acids are disclosed. In some embodiments, the isolated polynucleic acids are selected from the group consisting of (a) a polynucleic acid comprising a nucleic acid sequence set forth in Table 1 or Table 2; (b) a polynucleic acid comprising a nucleic acid sequence having at least about 90% identity to a nucleic acid sequence set forth in Table 1 or Table 2; and (c) a polynucleic acid capable of hybridizing under stringent conditions to a nucleic acid sequence set forth in Table 3 or Table 4, wherein the polynucleic acid is 50 nucleotides or shorter. Also disclosed are libraries of isolated polynucleic acids; kits for determining the presence of miRNA expressed in a sample; methods for detecting, quantifying, or both at least one miRNA directed against at least one specific gene present in a sample; methods for designing a polynucleotide primer sequence for amplifying a precursor miRNA (pre-miRNA), a mature miRNA, or both of interest; methods for detecting at least one sequence variation in at least one miRNA in a sample; computer program products including computer-executable instructions embodied in a computer-readable medium for performing the disclosed methods; and computer systems that in some embodiments include relational databases.
PCT/US2007/018478 2006-08-21 2007-08-21 Micro rna arrays and methods of using the same WO2008024343A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US82308006P 2006-08-21 2006-08-21
US60/823,080 2006-08-21
US89737807P 2007-01-25 2007-01-25
US60/897,378 2007-01-25

Publications (2)

Publication Number Publication Date
WO2008024343A2 WO2008024343A2 (en) 2008-02-28
WO2008024343A3 true WO2008024343A3 (en) 2008-11-06

Family

ID=39107347

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/018478 WO2008024343A2 (en) 2006-08-21 2007-08-21 Micro rna arrays and methods of using the same

Country Status (1)

Country Link
WO (1) WO2008024343A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101798594A (en) * 2009-11-13 2010-08-11 北京命码生科科技有限公司 Marker, detection method, biological chip and test box for detecting milk quality
CN112626220A (en) * 2021-01-18 2021-04-09 中国农业大学 Biomarker and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060105360A1 (en) * 2004-02-09 2006-05-18 Croce Carlo M Diagnosis and treatment of cancers with microRNA located in or near cancer associated chromosomal features

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060105360A1 (en) * 2004-02-09 2006-05-18 Croce Carlo M Diagnosis and treatment of cancers with microRNA located in or near cancer associated chromosomal features

Also Published As

Publication number Publication date
WO2008024343A2 (en) 2008-02-28

Similar Documents

Publication Publication Date Title
US11964997B2 (en) Methods of library construction for polynucleotide sequencing
AU2018201671B2 (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
JP5851496B2 (en) Modified stem-loop oligonucleotide-mediated reverse transcription and quantitative PCR with limited base spacing
Yan et al. One-step real time RT-PCR for detection of microRNAs
WO2008070144A3 (en) Imprinted genes and disease
Lan et al. Linear-hairpin variable primer RT-qPCR for MicroRNA
CN103571822B (en) A kind of multipurpose DNA fragmentation enriching method analyzed for new-generation sequencing
US20160215328A1 (en) Methods, compositions and systems for the analysis of nucleic acid molecules
Shaffer et al. miRNA profiling from blood—challenges and recommendations
Skurnikov et al. Profile of microRNA in Blood Plasma of Healthy Humans.
WO2008108843A3 (en) Methods and kits for amplifying dna
KR20160097193A (en) Multiplex Probes
WO2008024343A3 (en) Micro rna arrays and methods of using the same
CN1763223B (en) miRNA detection method
Zhou et al. A novel template repairing-PCR (TR-PCR) reaction platform for microRNA detection using translesional synthesis on DNA templates containing abasic sites
CN101886131B (en) Method of increasing specificity of nucleic acid hybridization using zwitterionic compound
EP3230477B1 (en) Methods for capturing nucleic acids
CN101633957A (en) Method and kit for detecting small RNA
WO2003072721A3 (en) Detection by sliding template amplification
EP1780289A3 (en) Method for assaying Reg IV mRNA
Ge et al. A universal linker-RT PCR based quantitative method for the detection of circulating miRNAs
EP1790719A4 (en) METHOD OF ASSAYING ALPHA 1,4-n-ACETYLGLUCOSAMINE TRANSFERASE (ALPHA 4GnT) mNRA
CN117487800A (en) sgrnas, compositions, kits and methods for detecting viruses
WO2016177774A1 (en) Method of quantifying mirnas using normalization
CN104232796A (en) RT-qPCR oligonucleotide for detecting Australia grapevine viroid, and detection method

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07837135

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

NENP Non-entry into the national phase

Ref country code: RU

122 Ep: pct application non-entry in european phase

Ref document number: 07837135

Country of ref document: EP

Kind code of ref document: A2