WO2008009867A3 - Epr-based detection, identification and quantification of free radicals, sampling device and kit - Google Patents

Epr-based detection, identification and quantification of free radicals, sampling device and kit Download PDF

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Publication number
WO2008009867A3
WO2008009867A3 PCT/FR2007/051704 FR2007051704W WO2008009867A3 WO 2008009867 A3 WO2008009867 A3 WO 2008009867A3 FR 2007051704 W FR2007051704 W FR 2007051704W WO 2008009867 A3 WO2008009867 A3 WO 2008009867A3
Authority
WO
WIPO (PCT)
Prior art keywords
free radicals
biological fluid
epr
quantification
kit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2007/051704
Other languages
French (fr)
Other versions
WO2008009867A2 (en
Inventor
Marcel Culcasi
Sylvia Pietri
Paul Tordo
Gaelle Gosset
Hakim Karoui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aix Marseille Universite
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite de Provence Aix Marseille I
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite de Provence Aix Marseille I filed Critical Centre National de la Recherche Scientifique CNRS
Publication of WO2008009867A2 publication Critical patent/WO2008009867A2/en
Publication of WO2008009867A3 publication Critical patent/WO2008009867A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/60—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using electron paramagnetic resonance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
    • G01N24/10—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using electron paramagnetic resonance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • High Energy & Nuclear Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for detecting, identifying or quantifying free radicals in a biological fluid, in which a biological fluid is sampled, said biological fluid is immediately mixed with a reactive composition containing a compound for trapping free radicals, a stabilizer and a nonoxidizing chelating agent, and then the mixture thus obtained is analysed using an analytical apparatus that detects a compound comprising a free electron, to a device for taking a sample of a biological fluid so as to immediately introduce it into a mixture with said reactive composition, and to kits for implementing the above method.
PCT/FR2007/051704 2006-07-20 2007-07-20 Epr-based detection, identification and quantification of free radicals, sampling device and kit Ceased WO2008009867A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0606623 2006-07-20
FR0606623A FR2904112A1 (en) 2006-07-20 2006-07-20 METHOD FOR DETECTING, IDENTIFYING AND QUANTIFYING FREE RADICALS, SAMPLING DEVICE AND KIT FOR CARRYING OUT SAID METHOD

Publications (2)

Publication Number Publication Date
WO2008009867A2 WO2008009867A2 (en) 2008-01-24
WO2008009867A3 true WO2008009867A3 (en) 2008-04-10

Family

ID=38007364

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2007/051704 Ceased WO2008009867A2 (en) 2006-07-20 2007-07-20 Epr-based detection, identification and quantification of free radicals, sampling device and kit

Country Status (2)

Country Link
FR (1) FR2904112A1 (en)
WO (1) WO2008009867A2 (en)

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BARTOSZ ET AL: "Use of spectroscopic probes for detection of reactive oxygen species", CLINICA CHIMICA ACTA, AMSTERDAM, NL, vol. 368, no. 1-2, June 2006 (2006-06-01), pages 53 - 76, XP005460736, ISSN: 0009-8981 *
BUETTNER GR, MASON RP: "Spin-trapping methods for detecting superoxide and hydroxyl free radicals in vitro and in vivo", CRITICAL REVIEWS OF OXIDATIVE STRESS AND AGING: ADVANCES IN BASIC SCIENCE, DIAGNOSTICS AND INTERVENTION, 2003, World scientific, New Jersey, London, Singapore, Hong Kong, pages 27 - 38, XP002433622 *
DIKALOVA AE ET AL: "An in vivo ESR spin-trapping study: Free radical generation in rats from formate intoxication - role of the Fenton reaction", PNAS, vol. 98, no. 24, 20 November 2001 (2001-11-20), pages 13549 - 13553, XP002433620 *
KADIISKA MB ET AL: "Iron supplementation generates hydroxyl radical in vivo - An ESR spin-trapping investigation", THE JOURNAL OF CLINICAL INVESTIGATION, vol. 96, September 1995 (1995-09-01), pages 1653 - 1657, XP002433621 *
KAROUI H ET AL: "ESR-spin trapping in the presence of cyclodextrins. Detection of PBN-superoxide spin adduct", TETRAHEDRON LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 45, no. 5, 26 January 2004 (2004-01-26), pages 1043 - 1045, XP004483320, ISSN: 0040-4039 *
KAROUI H ET AL: "Spin trapping of superoxide in the presence of beta-cyclodextrins", CHEM COMM, 2002, pages 3030 - 3031, XP002433623 *
KHAN N ET AL: "''Distant spin trapping'': a method for expanding the availability of spin trapping measurements", JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, AMSTERDAM, NL, vol. 62, no. 2, 28 February 2005 (2005-02-28), pages 125 - 130, XP004723347, ISSN: 0165-022X *

Also Published As

Publication number Publication date
WO2008009867A2 (en) 2008-01-24
FR2904112A1 (en) 2008-01-25

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