WO2008007702A1

WO2008007702A1 - Biosensor chip and process for manufacturing the same

Info

Publication number: WO2008007702A1
Application number: PCT/JP2007/063817
Authority: WO
Inventors: Shingo Kaimori; Takahiko Kitamura; Akira Harada; Toshifumi Hosoya; Isao Karube; Masao Gotoh; Hideaki Nakamura; Tsuyoshi Fujimura; Toshihisa Osaki; Tomoko Ishikawa
Original assignee: Sumitomo Electric Industries, Ltd.; National Institute Of Advanced Industrial Science And Technology
Priority date: 2006-07-14
Filing date: 2007-07-11
Publication date: 2008-01-17
Also published as: JP2008020391A

Abstract

A biosensor chip that reduces the collection amount of sample needed for measurement, thereby lessening the burden on user; and a process for manufacturing the same. Hollow reaction part (11) is provided with air guide passage (14), and the air guide passage (14) on its wall surface (15) is coated with surfactant (16), so that a sample can be easily led from sampling orifice (11a) provided at the distal end thereof into the hollow reaction part (11). In this instance, at least the vicinity of entrance (14a) of the air guide passage (14) may not be coated with the surfactant (16), so that entry of the sample into the air guide passage (14) would be hampered to thereby prevent any increase of the collection amount of sample by entry of the sample into the air guide passage (14) and accordingly lessen the burden on user. Further, specifically, at least the vicinity of air duct opening (18) of the air guide passage (14) may not be coated with the surfactant (16), so that even when the user by mistake intends to introduce blood in the air duct, introduction would be inhibited to thereby enable prevention of mismeasurement. Still further, specifically, a level difference may be provided between the entrance of the air guide passage (14) and the hollow reaction part (11), so that intrusion of the sample into the air guide passage would be prevented to thereby prevent any increase of the collection amount of sample and accordingly lessen the burden on user.

Description

Specification

Biosensor chip and manufacturing method thereof

Technical field

TECHNICAL FIELD [0001] The present invention relates to a biosensor chip and a method for manufacturing the same, for example, a biosensor chip capable of measuring and analyzing a chemical substance by collecting a sample from the tip of the biosensor chip and a method for manufacturing the same. is there.

Background art

Conventionally, a biosensor chip that collects a sample and measures and analyzes a chemical substance is known (see, for example, Patent Document 1).

As shown in FIG. 11, the biosensor chip 100 described in Patent Document 1 includes a first insulating substrate 101 and a second insulating substrate 102 laminated, and a pair of conductive substrates between the two insulating substrates 101 and 102. Sex trajectories 103 and 104 are provided. A notch 102 a is provided at one end of the second insulating substrate 102, and both conductive tracks 103 and 104 are exposed at one end of the biosensor chip 100. In addition, concave portions 105 and 106 are provided in the side edge portions on the other end side of both insulating substrates 101 and 102, respectively, and an opening portion 107 is provided in the concave portion 106 of the second insulating substrate 102. Yes. The opening 107 is provided with a reagent 108, and a lid member 112 having a recess 111 similar to the recess 106 is attached to the opening 107 via a hydrophilic coating 109! / .

Therefore, by pressing the concave portions 105, 106, 111 against the punctured finger or the like, the sample is guided to the reagent 108 in the opening 107, and measurement is performed.

Patent Document 1: Japanese Patent Application Laid-Open No. 2004-279433 (Fig. 1)

Disclosure of the invention

Problems to be solved by the invention

[0003] By the way, for people who have to measure many times a day, for example, diabetic patients, the burden increases when the amount of blood sample is increased. I want to reduce the amount! /

[0004] The present invention has been made in view of the above-mentioned problems, and its purpose is to collect samples. An object of the present invention is to provide a biosensor chip and a method of manufacturing the same that can prevent failure and reduce the burden on the user by reducing the amount of sample collected for measurement. Means for solving the problem

[0005] In order to achieve the above-described object, a biosensor chip as a first feature according to the present invention is a biosensor chip for measuring a substance contained in a sample, and is a hollow for measuring a sample. The reaction unit has an air conduction path that communicates with the sample collection port and communicates with the hollow reaction unit and an air hole opening on a side surface of the chip, and has an interface between the hollow reaction unit and the inner wall of the sample collection port. It is characterized in that an activator is applied and a surfactant is applied at least near the entrance of the air conduction path.

[0006] In the biosensor chip configured as described above, a surfactant is applied to the sample collection port and the wall surface of the hollow reaction part! /. Can be easily introduced into the hollow reaction part from the sampling port. At this time, since the surfactant is not applied near the entrance of the air conduction path, the sample is difficult to enter the air conduction path, and the amount of sample collected increases as the sample enters the air conduction path. It is possible to reduce the burden on the user.

[0007] In addition, the biosensor chip, which is the second feature of the present invention, is a biosensor chip that measures a substance contained in a sample, and the hollow reaction part that measures the sample is configured to collect the sample. An air conduction path that communicates with the mouth and communicates with the hollow reaction portion and the air hole opening on the side surface of the chip, and a surfactant is applied to the inner wall of the hollow reaction portion and the sampling port. And a surfactant is applied at least in the vicinity of the opening of the air hole in the air conduction path! /, !! /.

[0008] In the biosensor chip configured as described above, a surfactant is applied to the sample collection port and the wall surface of the hollow reaction part! /. Can be easily introduced into the hollow reaction part from the sampling port. In addition, by not applying a surfactant in the vicinity of the opening of the air hole, even if the user tries to inject blood into the air hole by mistake, it is not injected and erroneous measurement can be prevented.

[0009] The biosensor chip, which is the third feature of the present invention, is a biosensor chip for measuring a substance contained in a sample, and the hollow reaction part for measuring the sample is used for sampling. It has an air conduction path that communicates with the intake port and communicates with the hollow reaction part and the air hole opening on the side surface of the chip, and has a step between the hollow reaction part and the air conduction path. And

[0010] In the biosensor chip configured as described above, since the step is provided between the hollow reaction portion and the air conduction path, the intrusion of the sample at the step portion is prevented. Force It is difficult to enter the air conduction path, preventing the sample from entering the air conduction path and increasing the amount of sample collected, reducing the burden on the user.

[0011] In addition, the biosensor chip that is the fourth feature of the present invention has a step between the hollow reaction portion and the air conduction path in the first or second feature of the present invention. It is characterized by this.

[0012] In the biosensor chip configured as described above, a surfactant is applied to the sample collection port and the wall surface of the hollow reaction part, so that the sample is collected by the action of the surfactant that is compatible with the sample. It can be easily introduced into the hollow reaction part from the mouth. At this time, since the surfactant is not applied near the entrance of the air passage, the sample enters the air conduction path, and the amount of sample collected increases when the sample enters the air conduction path. It is possible to reduce the burden on the user. In addition, since there is a step between the hollow reaction part and the air conduction path, the sample can be prevented from entering at the step part, so that the sample entering the hollow reaction part is difficult to enter the air conduction path. It is possible to prevent the sample from entering the air passage and increase the amount of sample collected, thereby reducing the burden on the user. Furthermore, since there is this step when applying a surfactant or a reagent to the hollow reaction part, there is also an advantage that the surfactant or reagent can be applied without protruding from the hollow reaction part.

[0013] Alternatively, by not applying a surfactant in the vicinity of the opening of the air hole, even if a user mistakenly tries to put blood into the air hole, it is not injected and erroneous measurement can be prevented. Furthermore, since there is a step between the hollow reaction part and the air conduction path, the sample is prevented from entering at the step part, so it is difficult to enter the sample force air conduction path that entered the hollow reaction part. Prevents the amount of sample collected from entering the air conduction path, Can be reduced. Furthermore, since there is this step when applying a surfactant or applying a reagent to the hollow reaction part, there is also an advantage that the surfactant or reagent can be applied without protruding from the hollow reaction part.

[0014] In addition, the biosensor chip manufacturing method, which is a fifth feature of the present invention, is characterized in that a detection electrode is formed in a predetermined shape on one surface of a single electrically insulating flat substrate. The insulating layer and the adhesive layer and the spacer layer are formed in a predetermined shape, and the upper and lower substrates are formed by bending at the folding line of the flat substrate, and the bioconductive layer has an air conduction path communicating with the outside. A method of manufacturing a sensor chip, characterized in that a surfactant is not applied at least near the entrance of the air conduction path.

[0015] In the biosensor chip manufacturing method configured as described above, a biosensor chip is manufactured by folding a planar substrate on which a sensing electrode spacer layer is formed so that the surfaces of the adhesive layer are in contact with each other. . At this time, by not applying a surfactant in the vicinity of the entrance of the air conduction path previously provided in the hollow reaction part in the flat substrate, the sample enters the air conduction path and the amount of sample collected increases. It is possible to manufacture a biosensor chip that can prevent and reduce the burden on the user.

[0016] In addition, the biosensor chip manufacturing method, which is the sixth feature of the present invention, is characterized in that a detection electrode is formed in a predetermined shape on one surface of a single electrically insulating flat substrate. The insulating layer and the adhesive layer and the spacer layer are formed in a predetermined shape, and the upper and lower substrates are formed by bending at the folding line of the flat substrate, and the bioconductive layer has an air conduction path communicating with the outside. A method for manufacturing a sensor chip, characterized in that a surfactant is not applied at least in the vicinity of the air hole opening of the air conduction path.

In the biosensor chip manufacturing method configured as described above, a biosensor chip is manufactured by folding a planar substrate on which a sensing electrode spacer layer is formed so that the surfaces of the adhesive layer are in contact with each other. . At this time, by not applying a surfactant in the vicinity of the opening of the air hole, a biosensor chip is manufactured that prevents erroneous measurement by preventing the user from injecting blood into the air hole by mistake. Is possible.

[0018] Further, the biosensor chip manufacturing method, which is the seventh feature of the present invention, is characterized in that a detection electrode is formed in a predetermined shape on one surface of a single electrically insulating flat substrate. A biosensor chip having an air conduction path communicating with the outside is formed by forming an insulating layer, an adhesive layer, and a spacer layer in a predetermined shape, and bending the flat substrate to form upper and lower substrates. It is a manufacturing method, Comprising: A level | step difference is provided between the said hollow reaction part and the said air conduction path, It is characterized by the above-mentioned.

[0019] In the biosensor chip manufacturing method configured as described above, a biosensor chip is manufactured by folding back a flat substrate on which a sensing electrode spacer layer is formed so that the surfaces of the adhesive layer are in contact with each other. . At this time, since a sample is prevented from entering the step portion by providing a step between the hollow reaction portion and the air passage in advance on the flat substrate, the sample enters the air conduction path and the amount of sample collected is reduced. It is possible to prevent the increase and reduce the burden on the user. In addition, there is an advantage that the surfactant and the reagent can be applied without protruding from the hollow reaction part because there is this step when the surfactant is applied to the hollow reaction part or the reagent is applied.

The invention's effect

[0020] According to the present invention, since the sample is collected from the tip of the biosensor chip, it is possible to prevent failure when collecting the sample, and a surfactant is applied in the vicinity of the entrance of the air conduction path. Therefore, it is difficult for the sample to enter the air conduction path, preventing the sample from entering the air conduction path and increasing the amount of sample collected, and reducing the burden on the user. Is obtained. Alternatively, by not applying a surfactant in the vicinity of the air hole opening, even if the user tries to accidentally put blood into the air hole, it is not injected and erroneous measurement can be prevented. Alternatively, it is possible to prevent an increase in the amount of sample collected by providing a step in the sample at the entrance of the air conduction path in the inner wall of the hollow reaction part.

Brief Description of Drawings

FIG. 1 (A) is a plan view showing a biosensor chip according to a first embodiment of the present invention.

(B) is a side view seen from the B direction in FIG. 1 (A). (C) is an end view as seen from the direction C in FIG.

FIG. 2 (A) is a perspective view of a hollow reaction part and an air conduction path. (B) is a cross-sectional view taken along the line BB in FIG. 2 (A). (C) is a cross-sectional view taken along the line CC in FIG. 2 (A). FIG. 3 is a plan view showing a planar substrate showing a step of providing a detection electrode in the biosensor chip manufacturing method according to the first embodiment.

4] A plan view showing a planar substrate showing a process of providing an insulating layer in the method of manufacturing a biosensor chip according to the first embodiment.

FIG. 5 is a plan view showing a planar substrate showing a process of providing an adhesive layer in the biosensor chip manufacturing method according to the first embodiment.

6] (A) is a plan view showing a biosensor chip according to a second embodiment of the present invention. (B) is a side view seen from the B direction in FIG. 6 (A). (C) is an end view as seen from the direction C in FIG.

Garden 7] (A) is a perspective view of the hollow reaction part and the air conduction path. (B) is a cross-sectional view of position BB in FIG. 7 (A). (C) is a cross-sectional view taken along the line CC in FIG. 7 (A).

FIG. 8 is a plan view showing a planar substrate showing a step of providing a detection electrode in the biosensor chip manufacturing method according to the second embodiment.

9] FIG. 9 is a plan view showing a planar substrate showing the process of providing an insulating layer in the biosensor chip manufacturing method according to the second embodiment.

[10] FIG. 10 is a plan view showing a planar substrate showing a process of providing an adhesive layer in the method of manufacturing a biosensor chip according to the second embodiment.

11] An exploded perspective view showing a conventional biosensor chip.

Explanation of symbols

10A, 10B biosensor chip

11 Hollow reaction part

11a Sampling port

12a, 12b detection electrode

13 Reagents

14 Air conduction path

14a entrance

15 inner wall

16 Surfactant 18 Air hole opening

20 Planar substrate

20a one surface

23 Insulation layer

24 Folded curve

25 Adhesive layer

26 Spacer layer

BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, embodiments according to the present invention will be described in detail with reference to the drawings.

Fig. 1 (A) is a plan view showing the biosensor chip according to the first embodiment of the present invention, Fig. 1 (B) is a side view of Fig. 1 (A) viewed from the B direction, and Fig. 1 (C) is a diagram. Fig. 2 (A) is a perspective view of the hollow reaction part and the air conduction path, Fig. 2 (B) is a cross-sectional view at the BB position in Fig. 2 (A), and Fig. 2 (C). Fig. 2 is a cross-sectional view at the CC position in Fig. 2 (A).

As shown in FIG. 1, the biosensor chip 10A according to the first embodiment of the present invention has a sample collection port 1 la opened at the tip. It is preferable that the sample collection port is provided at the tip of the biosensor chip because the sample can be collected easily. The biosensor chip 10A according to the first embodiment of the present invention has a hollow reaction part 11 having a detection electrode 12 and a reagent 13 continuously in the sample collection port 11a, and further has a hollow reaction part. 11 has an air passage 14 that communicates with the outside. As shown in FIG. 2, a surfactant 16 is applied to the sample collection port 11a and the inner wall 15 of the hollow reaction part 11.

The sample collected from the sample collection port 11 a flows into the hollow reaction unit 11 due to the presence of the air conduction path 14 and reacts with the reagent 13. The surfactant 16 also facilitates the introduction of the sample into the hollow reaction part 11.

As shown in FIGS. 1A to 1C, the biosensor chip 10A has a laminated structure in which a spacer layer 26 is sandwiched between a lower substrate 21 and an upper substrate 22. Biosensor chip 1 The front end of the OA is formed in a narrow shape, the spacer layer 26 is notched inside to form a hollow reaction part 11, and the tip of the hollow reaction part 11 is the sample The sampling port is 11a. Lower substrate A pair of detection electrodes 12a and 12b are provided on the upper surface of 21 and bend toward the side closer to each other at the tip of the biosensor chip 10A and face each other at a predetermined interval in the hollow reaction part 11. A reagent 13 that reacts with the sample is provided between or in the vicinity of the detection electrodes 12a and 12b facing each other in the hollow reaction part 11. Further, at the rear end portion of the biosensor chip 10A, the lower substrate 21 protrudes from the upper substrate 22 and the spacer layer 26. On the upper surface of the lower substrate 21, the rear end portions of the detection electrodes 12a and 12b are the upper surface. Is exposed. In the present invention, the spacer layer includes an insulating layer and an adhesive layer.

[0027] As shown in FIG. 1, an air conducting path 14 communicating the hollow reaction section 11 and the outside is provided at the rear end of the hollow reaction section 11 (the left end in FIGS. 1 (A) and 1 (B)). Is provided. As shown in FIG. 2, the air conduction path 14 is provided so that the shape of the combination of the air conduction path 14 and the hollow reaction portion 11 is T-shaped so as to penetrate the biosensor chip 10A from side to side. Is desirable. The air hole opening 18 of the air conduction path is provided on the side surface of the biosensor chip. A surfactant 16 is applied to the inner wall 15 of the hollow reaction part 11. Here, the inner wall 15 is applied not only to the bottom surface 15a of the hollow reaction part 11 (the upper surface of the lower substrate 21, see FIG. 2 (B)) but also to the side surface 15b (see FIG. 2 (C)) and the upper surface. Therefore, it is desirable that there is no need to consider the directionality when using the biosensor chip 10A.

[0028] Thus, since the surfactant 16 is applied to the inner wall 15 of the hollow reaction part 11, the sample can be easily introduced into the hollow reaction part 11 from the sample sampling port 11a at the tip. Since the surfactant 16 is not applied in the vicinity of the inlet 14a of the force / air conduction path 14, the sample is difficult to enter the air conduction path 14. For this reason, it is possible to prevent the sample from being collected by entering the air conduction path 14, and to reduce the burden on the user.

[0029] Alternatively, since the surfactant 16 is applied to the inner wall 15 of the hollow reaction portion 11, the sample can be easily removed from the sampling port 11a at the tip by the action of the surfactant that is compatible with the sample. However, since the surfactant 16 is not applied in the vicinity of the air hole opening 18 of the air conduction path 14, the user accidentally tries to put blood into the air hole. Is not injected, and erroneous measurement can be prevented.

Also, if the depth dimension L1 and the width dimension L2 in the longitudinal direction where the sample of the hollow reaction part 11 communicating with the sampling port 11a flows, the ratio L1 / L2 is set to 1.8 or less. Yes. As a result, the amount of sample collected can be reduced to a small amount of 0.31 or less, and the measurement accuracy can be increased with a shorter depth dimension.

[0030] Next, a manufacturing method of the above-described biosensor chip 10A will be described.

FIG. 3 is a plan view showing a planar substrate showing a process of providing detection electrodes in the biosensor chip manufacturing method according to the first embodiment, and FIG. 4 shows an insulating layer in the biosensor chip manufacturing method according to the first embodiment. FIG. 5 is a plan view showing a flat substrate showing a step of providing an adhesive layer in the biosensor chip manufacturing method according to the first embodiment.

First, as shown in FIG. 3, a plurality of biosensor chips 10A can be formed at one time by using a single electrically insulating flat substrate 20. The planar substrate 20 can be made of, for example, polyethylene terephthalate (PET). A folding line 24 is provided on the left and right at the center of the flat substrate 20, and a lower substrate 21 is disposed below the folding line 24 (on the lower side in FIG. 3) with the tip portion facing upward. On the upper side of the curve 24, the upper substrate 22 is arranged with the tip portion down. The length of the lower substrate 21 is longer than the length of the upper substrate 22, and when the flat substrate 20 is folded back at the folding line 24 and overlapped, as described above in FIG. The upper surface is exposed from the rear end of the upper substrate 22. Detection electrodes 12a and 12b are formed in a predetermined shape (see FIG. 1A) on the upper surface of the lower substrate 21 on one surface 20a of the planar substrate 20. The detection electrodes 12a and 12b can be formed, for example, by printing a carbon in a predetermined shape.

Subsequently, as shown in FIG. 4, an insulating layer 23 is provided on the planar substrate 20 on which the detection electrodes 12a and 12b are provided. The insulating layer 23 includes a narrow portion 24a along the folding line 24, a portion 23a corresponding to the hollow reaction portion 11, a 23b corresponding to the air conduction path 14, and a rear end of the lower substrate 21 exposed from the upper substrate 22. It is formed on the entire surface except for the portion 21a. Then, a surfactant 16 is applied to the part corresponding to the hollow reaction part 11 (see FIG. 2).

Subsequently, as shown in FIG. 5, an adhesive layer 25 is formed on the insulating layer 23. The adhesive layer 25 is for folding the flat substrate 20 along the folding line 24 and integrating the upper and lower substrates 21 and 22 via the insulating layer 23, and an adhesive can be used. The flat substrate 20 is folded along the folding line 24 and overlapped so that the insulating layer 23 and the adhesive layer 25 are spaced apart. The hollow reaction portion 11 is formed by the portion 23a where the spacer layer is not provided, and the air conduction path 14 is formed by the portion 23b where the spacer layer such as the insulating layer 23 and the adhesive layer 25 is not provided. become. That is, since the spacer layer such as the insulating layer 23 and the adhesive layer 25 is not provided in the hollow reaction part 11 and the air conduction path 14, the spacer layer such as the two insulating layers 23 and the two adhesive layers 25 is provided. A space with a total height of all the thicknesses is formed. The surfactant 16 applied after the formation of the insulating layer 23 can also be applied after the formation of the adhesive layer 25.

[0034] Thereafter, the planar substrate 20 is bent along the folding line 24, and unnecessary portions are cut to cut out each biosensor chip 10A to form a biosensor chip. Alternatively, the biosensor chip 10A can also be formed by cutting first while leaving a portion along the folding line 24, then bending along the folding line 24, and then cutting along the folding line.

[0035] As shown in FIG. 4, when several biosensor chips are manufactured at a time by providing a shape in which the air conduction path 14 and the hollow reaction part 11 are combined in a T shape, It is possible to provide an air conduction path 14 between adjacent biosensor chips at a time, and it is possible to manufacture a biosensor chip with high manufacturing efficiency.

[0036] According to the biosensor chip 10A and the manufacturing method thereof described above, the surfactant 16 is applied to the wall surface 15 of the hollow reaction part 11, so that it is provided at the tip of the hollow reaction part 11! / Therefore, it is possible to easily introduce the sample collected from the sample collection port 1 la into the hollow reaction part 11. At this time, since the surfactant 16 is not applied in the vicinity of the inlet 14a of the air conduction path 14, the sample is prevented from reaching the air conduction path 14 and is difficult to enter the air conduction path 14. For this reason, it is possible to prevent the sample from entering the air conduction path 14 and to increase the amount of sample collected, and to reduce the burden on the user. Alternatively, by not applying a surfactant in the vicinity of the air hole opening 18, even if the user tries to accidentally put blood into the air hole, it is not injected and erroneous measurement can be prevented.

[0037] Next, a second embodiment according to the present invention will be described in detail with reference to the drawings.

FIG. 6 (A) is a plan view showing a biosensor chip according to the second embodiment of the present invention, FIG. 6 (B) is a side view seen from the direction B in FIG. 6 (A), and FIG. Fig. 7 (A) is a perspective view of the hollow reaction part and the air conduction path, and Fig. 7 (B) is B in Fig. 7 (A). FIG. 7C is a cross-sectional view at position B, and FIG. 7C is a cross-sectional view at position CC in FIG. 7A. In the first embodiment, parts that are the same as the parts described above are given the same reference numerals, and redundant descriptions are omitted.

[0038] As shown in Fig. 6, the biosensor chip 10B according to the second embodiment of the present invention has a sample sampling port 11a opened at the tip, and a detection electrode continuously connected to the sample sampling port 11a. 12 and a hollow reaction part 11 having a reagent 13, and further an air conduction path 14 communicating the hollow reaction part 11 and the outside. Then, as shown in FIG. 7, a step 17 was provided at the inlet 14a of the air conduction path 14 in the inner wall 15 of the hollow reaction part 11.

[0039] As described above, since the air conducting path 14 is provided in the hollow reaction part 11, and the step 17 is provided in the inlet 14a of the air conducting path 14, the intrusion of the sample is prevented at the step part, and the sample is air It is difficult to enter the conduction path 14. For this reason, it is possible to prevent an increase in the amount of sample collected due to the sample entering the air conduction path 14, thereby reducing the burden on the user. In addition, there is an advantage that the surfactant and the reagent can be applied without protruding from the hollow reaction part because there is this step when the surfactant is applied to the hollow reaction part or the reagent is applied.

Further, if the depth dimension, which is the longitudinal direction in which the sample of the hollow reaction part 11 communicating with the sample collection port 11a flows, is L1, and the width dimension is L2, the ratio L1 / L2 is set to 1.8 or less. As a result, the amount of sample collected can be reduced to a small amount of 0.31 or less, and the measurement accuracy can be increased with a shorter depth dimension.

[0040] Next, a manufacturing method of the above-described biosensor chip 10B will be described.

FIG. 8 is a plan view showing a planar substrate showing a process of providing detection electrodes in the method for manufacturing a biosensor chip according to the second embodiment, and FIG. 9 shows an insulating layer in the method for manufacturing the biosensor chip according to the second embodiment. FIG. 10 is a plan view showing a planar substrate showing a process of providing an adhesive layer in the biosensor chip manufacturing method according to the second embodiment. Note that the manufacturing method of the biosensor chip 10B is mostly the same as the manufacturing method of the biosensor chip 10A according to the first embodiment, and therefore, it is attached to common processes.

First, as shown in FIG. 8, the central portion of the flat substrate 20 has a horizontal direction (left and right in FIG. 8). A lower substrate 21 is disposed on the lower side of the folding line 24 provided with the front end portion facing up, and an upper substrate 22 is disposed on the upper side of the folding line 24 with the front end portion facing down. The length of the lower substrate 21 is longer than the length of the upper substrate 22. The detection electrodes 12a and 12b are formed in a predetermined shape (see FIG. 6 (A)) on the upper surface of the lower substrate 21 on one surface 20a of the flat substrate 20.

Subsequently, as shown in FIG. 9, an insulating layer 23 is provided on the planar substrate 20 on which the detection electrodes 12a and 12b are provided. At this time, the insulating layer 23 is provided except for the part corresponding to the hollow reaction part 11. As a result, as shown in FIG. 7 (C), the portion corresponding to the hollow reaction portion 11 is lower than the other portions by the thickness of the insulating layer 23, so that the hollow reaction portion 11 and the hollow reaction portion 11 A step 17 is formed between the inlet 14a of the air conduction path 14 provided continuously at the rear end of the air passage.

Subsequently, as shown in FIG. 10, an adhesive layer 25 is formed on the insulating layer 23. At this time, the adhesive layer 25 is not provided on the insulating layer 23b in the portion corresponding to the air reaction portion 11 and the portion corresponding to the air conduction path. Then, the flat substrate 20 is bent along the folding line 24 and the adhesive layer 25 is bonded. Then, unnecessary portions are cut to manufacture the biosensor chip 10B.

Thus, the thickness of the sensor chip 10B is the sum of the thicknesses of the spacer layers such as the two substrates 21, 22, the two insulating layers 23, and the two adhesive layers 25 as a whole. In addition, since the spacer layer 11 such as the insulating layer 23 and the adhesive layer 25 is not provided in the space reaction portion 11, the thickness of the spacer layer such as the two insulating layers 23 and the two adhesive layers 25 is determined. A space with the total height is formed. Furthermore, since only the adhesive layer 25 is not provided in the air conduction path 14, a space having a height equal to the thickness of the two adhesive layers 25 is formed. As a result, a step 17 corresponding to the thickness of the insulating layer 23 is formed at the boundary between the hollow reaction portion 11 and the air conduction path 14.

As described above, according to the biosensor chip 10B and the manufacturing method thereof according to the second embodiment described above, the step 17 between the hollow reaction part 11 and the air conduction path 14 is, as shown in FIG. By providing the insulating layer 23 in the portion corresponding to the passage 14, it can be easily provided, and the step 17 is the same as the case of the biosensor chip 10A according to the first embodiment described above. Similarly, it is possible to prevent the sample from entering the air conduction path 14 and increase the amount of sample collected, thereby reducing the burden on the user.

[0045] As shown in FIG. 9, by providing the air conduction path 14 and the hollow reaction portion 11 so as to be on the shape force, adjacent to each other when several biosensor chips are manufactured at one time. It is possible to provide an air conduction path 14 between matching biosensor chips at a time, and it is possible to manufacture a biosensor chip with high manufacturing efficiency.

Note that the biosensor chip and the manufacturing method thereof according to the present invention are not limited to the above-described embodiments, and can be appropriately modified and improved.

For example, the range in which the surfactant 16 is applied in the first embodiment described above and the position where the step 17 is provided in the second embodiment are not limited to those described above. It suffices if it can be prevented from entering the conduction path 14. In addition, as a reagent to be applied to the wall surface of the inner wall of the hollow reaction portion other than the vicinity of the entrance of the air conduction path, a substance other than the surfactant is also used as appropriate.

In the second embodiment, it is also possible to adopt a structure in which a sample is collected from the side surface that is not from the tip of the biosensor chip.

[0047] Although the present invention has been described in detail and with reference to specific embodiments, it will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the invention. It is.

This application is based on a Japanese patent application filed on July 14, 2006 (Japanese Patent Application No. 2006-193886), the contents of which are incorporated herein by reference.

Industrial applicability

[0048] As described above, in the biosensor chip and the manufacturing method thereof according to the present invention, since the surfactant is not applied in the vicinity of the entrance of the air conduction path, the sample does not easily enter the air conduction path. Therefore, the sample can be prevented from increasing in volume by entering the air conduction path, and the burden on the user can be reduced. Or, since the surfactant is not applied at least near the air hole opening of the air passage, even if the user tries to put blood into the air hole by mistake, it will not be injected and erroneous measurement can be prevented. . Alternatively, by having a step between the hollow reaction part and the air conduction path, the sample is It is difficult to enter the air conduction path, and it is possible to reduce the burden on the user by preventing the sample from entering the air conduction path and increasing the amount of sample collected. Therefore, it is useful as a biosensor chip that can collect a sample from the tip of the biosensor chip and perform chemical substance measurement and analysis, and a manufacturing method thereof.

Claims

The scope of the claims

[1] A biosensor chip for measuring a substance contained in a sample,

The hollow reaction part for measuring the sample communicates with the sample collection port,

An air conduction path that communicates the hollow reaction part and the air hole opening on the side surface of the chip;

A biosensor chip, wherein a surfactant is applied to the hollow reaction part and an inner wall of the sampling port, and a surfactant is not applied at least near the entrance of the air conduction path.

[2] A biosensor chip for measuring a substance contained in a sample,

A biosensor characterized in that a surfactant is applied to the hollow reaction part and the inner wall of the sampling port, and no surfactant is applied at least in the vicinity of the air hole opening of the air conduction path. Chip.

[3] A biosensor chip for measuring a substance contained in a sample,

A biosensor chip comprising a step between the hollow reaction part and the air conduction path.

[4] The biosensor chip according to [1] or [2], wherein there is a step between the hollow reaction part and the air conduction path.

[5] A detection electrode is formed in a predetermined shape on one surface of a single flat substrate, and a spacer layer is formed in a predetermined shape, and the upper and lower substrates are bent at a folding line of the flat substrate. A method of manufacturing a biosensor chip having an air conducting path formed and communicated with a hollow reaction part and the outside,

A surfactant is not applied at least near the entrance of the air conduction path. A method for producing a biosensor chip.

[6] A detection electrode is formed in a predetermined shape on one surface of a flat substrate, a spacer layer is formed in a predetermined shape, and the upper and lower substrates are bent at a folding line of the flat substrate. A method of manufacturing a biosensor chip having an air conducting path formed and communicated with a hollow reaction part and the outside,

A biosensor chip manufacturing method, wherein a surfactant is not applied at least in the vicinity of an air hole opening of the air conduction path.

[7] A detection electrode is formed in a predetermined shape on one surface of a single flat substrate, and a spacer layer is formed in a predetermined shape, and the upper and lower substrates are bent at a folding line of the flat substrate. A method of manufacturing a biosensor chip having an air conducting path formed and communicated with a hollow reaction part and the outside,

A method for producing a biosensor chip, comprising providing a step between the hollow reaction part and the air conduction path.