WO2008002329A3 - Isolement et extension de cellules animales dans des cultures cellulaires - Google Patents

Isolement et extension de cellules animales dans des cultures cellulaires Download PDF

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Publication number
WO2008002329A3
WO2008002329A3 PCT/US2006/049500 US2006049500W WO2008002329A3 WO 2008002329 A3 WO2008002329 A3 WO 2008002329A3 US 2006049500 W US2006049500 W US 2006049500W WO 2008002329 A3 WO2008002329 A3 WO 2008002329A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
stem
cell
expansion
methods
Prior art date
Application number
PCT/US2006/049500
Other languages
English (en)
Other versions
WO2008002329A2 (fr
Inventor
Scheffer Tseng
Hua He
Wei Li
Ying-Ting Chen
Yasutaka Hayashida
Original Assignee
Tissuetech Inc
Scheffer Tseng
Hua He
Wei Li
Ying-Ting Chen
Yasutaka Hayashida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/476,376 external-priority patent/US20070010008A1/en
Application filed by Tissuetech Inc, Scheffer Tseng, Hua He, Wei Li, Ying-Ting Chen, Yasutaka Hayashida filed Critical Tissuetech Inc
Publication of WO2008002329A2 publication Critical patent/WO2008002329A2/fr
Publication of WO2008002329A3 publication Critical patent/WO2008002329A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Ophthalmology & Optometry (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des procédés d'isolement, de purification et d'extension de cellules souches animales et de cellules de type souche. Les procédés d'isolement incluent des conditions permettant de digérer préférentiellement des cellules n'étant pas des cellules souches ou de type souche dans une population et de provoquer préférentiellement l'adhérence de cellules souches et de cellules de type souche dans une population. Les procédés d'extension consistent à cultiver de telles cellules dans des conditions permettant de moduler le signal TGF-β, d'inhiber le signal cellulaire médié par la p38 MAP kinase au moyen d'inhibiteurs de petite masse moléculaire, de provoquer l'extension des cellules sur des cellules épithéliales amniotiques humaines utilisées comme couches nourricières, de réguler la densité de l'ensemencement des cellules, de réguler les niveaux de Ca2+ dans les milieux de culture, de provoquer l'adhérence rapide sur un substrat, ou à cultiver les cellules dans lesdites conditions combinées. Plus particulièrement, l'invention concerne des procédés et des systèmes permettant l'extension de cellules animales dans des cultures cellulaires ex vivo tout en prévenant une différenciation cellulaire, et en enrichissant de manière sélective des cellules souches. Les modes de réalisation portent également sur un système de culture permettant l'extension ex vivo de cellules épithéliales ou de cellules mésenchymateuses limbiques, aussi bien que des greffes chirurgicales pratiquées au départ de ces cellules.
PCT/US2006/049500 2006-05-17 2006-12-28 Isolement et extension de cellules animales dans des cultures cellulaires WO2008002329A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US80149106P 2006-05-17 2006-05-17
US60/801,491 2006-05-17
US11/476,376 2006-06-28
US11/476,376 US20070010008A1 (en) 2005-06-29 2006-06-28 Ex vivo expansion of primary animal cells

Publications (2)

Publication Number Publication Date
WO2008002329A2 WO2008002329A2 (fr) 2008-01-03
WO2008002329A3 true WO2008002329A3 (fr) 2008-07-17

Family

ID=38846144

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/049500 WO2008002329A2 (fr) 2006-05-17 2006-12-28 Isolement et extension de cellules animales dans des cultures cellulaires

Country Status (1)

Country Link
WO (1) WO2008002329A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011072461A1 (fr) * 2009-12-18 2011-06-23 Shanghai He Chen Biotechnology Co., Ltd. Matériels et méthodes pour la production de cellules souches pluripotentes
JP6218229B2 (ja) * 2011-12-28 2017-10-25 京都府公立大学法人 角膜内皮細胞の培養正常化
US9664671B2 (en) 2012-07-24 2017-05-30 Nissan Chemical Industries, Ltd. Culture medium composition and method of culturing cell or tissue using thereof
EP2889368B1 (fr) * 2012-08-23 2019-12-18 Nissan Chemical Corporation Accélérateur de production de protéine
US10017805B2 (en) 2012-08-23 2018-07-10 Nissan Chemical Industries, Ltd. Enhancing ingredients for protein production from various cells
US10125349B2 (en) 2013-03-28 2018-11-13 Ge Healthcare Bio-Sciences Ab Method for cell culture
US9944895B2 (en) 2013-03-28 2018-04-17 Ge Healthcare Bio-Sciences Ab Method for cell culture

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007002900A1 (fr) * 2005-06-29 2007-01-04 Tissue Tech, Inc. Expansion ex vivo de cellules animales primaires

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007002900A1 (fr) * 2005-06-29 2007-01-04 Tissue Tech, Inc. Expansion ex vivo de cellules animales primaires

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BONFIELD W. ET AL.: "Designing porous scaffolds for tissue engineering", PHILOSOPHICAL TRANSACTIONS. SERIES A, MATHEMATICAL, PHYSICAL, AND ENGINEERING SCIENCES, vol. 364, no. 1838, January 2006 (2006-01-01), pages 227 - 232 *
KAWAKITA T. ET AL.: "Keratocan Expression of Murine Keratocytes Is Maintained on Amniotic Membrane by Down-regulating Transforming Growth Factor-beta Signaling", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 280, no. 29, 2005, pages 27058 - 27092 *
KAWAKITA T. ET AL.: "Preservation and Expansion of the Primate Keratocyte Phenotype by Downregulating TGF-beta Signaling in a Low-Calcium, Serum-Free Medium", INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, vol. 47, no. 5, May 2006 (2006-05-01), pages 1918 - 1927 *
YAM H.F. ET AL.: "Growth Factor Changes in Ex Vivo Expansion of Human Limbal Epithelial Cells on Human Amniotic Membrane", CORNEA, vol. 21, no. 1, 2002, pages 101 - 105 *
YEH L.K. ET AL.: "Soluble Lumican Glycoprotein Purified from Human Amniotic Membrane Promotes Corneal Epithelial Wound Healing", INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, vol. 46, no. 2, 2005, pages 479 - 486 *

Also Published As

Publication number Publication date
WO2008002329A2 (fr) 2008-01-03

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