WO2007149791A3 - System for isolating biomolecules from a sample - Google Patents

System for isolating biomolecules from a sample Download PDF

Info

Publication number
WO2007149791A3
WO2007149791A3 PCT/US2007/071402 US2007071402W WO2007149791A3 WO 2007149791 A3 WO2007149791 A3 WO 2007149791A3 US 2007071402 W US2007071402 W US 2007071402W WO 2007149791 A3 WO2007149791 A3 WO 2007149791A3
Authority
WO
WIPO (PCT)
Prior art keywords
cartridge
fluids
purification
sample
isolating biomolecules
Prior art date
Application number
PCT/US2007/071402
Other languages
French (fr)
Other versions
WO2007149791A2 (en
Inventor
Jeffrey C Braman
Lee Scott Basehore
Natalia Novoradovskaya
Original Assignee
Stratagene Inc
Jeffrey C Braman
Lee Scott Basehore
Natalia Novoradovskaya
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratagene Inc, Jeffrey C Braman, Lee Scott Basehore, Natalia Novoradovskaya filed Critical Stratagene Inc
Priority to EP07812176A priority Critical patent/EP2035540A2/en
Priority to JP2009530708A priority patent/JP2009540868A/en
Publication of WO2007149791A2 publication Critical patent/WO2007149791A2/en
Publication of WO2007149791A3 publication Critical patent/WO2007149791A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Abstract

The present invention provides an automated system for purification of a substance of interest. The system generally comprises an instrument for moving fluids through the system, a reagent pack for storing fluids, and a purification cartridge. The cartridge comprises two filtration units for binding substances based on different physical properties. The cartridge also comprises rotary valves for control of movement of fluids on the cartridge. In preferred embodiments, the system is useful for purifying RNA from blood samples.
PCT/US2007/071402 2006-06-15 2007-06-15 System for isolating biomolecules from a sample WO2007149791A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP07812176A EP2035540A2 (en) 2006-06-15 2007-06-15 System for isolating biomolecules from a sample
JP2009530708A JP2009540868A (en) 2006-06-15 2007-06-15 System for isolating biomolecules from samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81462206P 2006-06-15 2006-06-15
US60/814,622 2006-06-15

Publications (2)

Publication Number Publication Date
WO2007149791A2 WO2007149791A2 (en) 2007-12-27
WO2007149791A3 true WO2007149791A3 (en) 2008-11-27

Family

ID=38834277

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/071402 WO2007149791A2 (en) 2006-06-15 2007-06-15 System for isolating biomolecules from a sample

Country Status (4)

Country Link
US (1) US20080026451A1 (en)
EP (1) EP2035540A2 (en)
JP (1) JP2009540868A (en)
WO (1) WO2007149791A2 (en)

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Also Published As

Publication number Publication date
US20080026451A1 (en) 2008-01-31
WO2007149791A2 (en) 2007-12-27
EP2035540A2 (en) 2009-03-18
JP2009540868A (en) 2009-11-26

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