WO2007147527A3 - Apiase-proteinkonstrukt - Google Patents

Apiase-proteinkonstrukt Download PDF

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Publication number
WO2007147527A3
WO2007147527A3 PCT/EP2007/005301 EP2007005301W WO2007147527A3 WO 2007147527 A3 WO2007147527 A3 WO 2007147527A3 EP 2007005301 W EP2007005301 W EP 2007005301W WO 2007147527 A3 WO2007147527 A3 WO 2007147527A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
amino acid
acid sequence
tag
apiase
Prior art date
Application number
PCT/EP2007/005301
Other languages
English (en)
French (fr)
Other versions
WO2007147527A2 (de
Inventor
Guenter Fischer
Cordelia Schiene-Fischer
Gerhard Kuellertz
Original Assignee
Max Planck Gesellschaft
Guenter Fischer
Cordelia Schiene-Fischer
Gerhard Kuellertz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft, Guenter Fischer, Cordelia Schiene-Fischer, Gerhard Kuellertz filed Critical Max Planck Gesellschaft
Publication of WO2007147527A2 publication Critical patent/WO2007147527A2/de
Publication of WO2007147527A3 publication Critical patent/WO2007147527A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Die Erfindung betrifft ein Proteinkonstrukt zur Herstellung löslicher Polypeptide. Um eine Möglichkeit bereitzustellen, mit welcher sich auf einfache und damit kostengünstige Weise verschiedenste Polypeptide in löslicher Form herstellen lassen wird ein Proteinkonstrukt vorgeschlagen, umfassend ein Fusionsprotein mit einem ersten Aminosäuresequenzabschnitt und einem zweiten Aminosäuresequenzabschnitt, wobei der erste Aminosäure-sequenzabschnitt eine Sekundär-Amid Peptidbindungs spezifische cis/trans Isomerase (APIase) kodiert und der zweite Aminosäuresequenzabschnitt ein beliebiges erstes Polypeptid kodiert, oder umfassend ein Polypeptidassoziat, enthaltend ein mit einem ersten Tag versehenes erstes Polypeptidmolekül und ein mit einem zweiten Tag versehenes zweites Polypeptidmolekül, wobei das erste Polypeptidmolekül eine APIase ist und das zweite Polypeptidmolekül ein beliebiges zweites Polypeptid ist und wobei der erste Tag an dem zweiten Tag spezifisch bindet, vorzugsweise mit einer thermodynami sehen Affinitätskonstante von kleiner als 1 μM.
PCT/EP2007/005301 2006-06-19 2007-06-15 Apiase-proteinkonstrukt WO2007147527A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006028076.8 2006-06-19
DE200610028076 DE102006028076A1 (de) 2006-06-19 2006-06-19 APlase-Proteinkonstrukt

Publications (2)

Publication Number Publication Date
WO2007147527A2 WO2007147527A2 (de) 2007-12-27
WO2007147527A3 true WO2007147527A3 (de) 2008-03-27

Family

ID=38690317

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/005301 WO2007147527A2 (de) 2006-06-19 2007-06-15 Apiase-proteinkonstrukt

Country Status (2)

Country Link
DE (1) DE102006028076A1 (de)
WO (1) WO2007147527A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9580737B2 (en) * 2013-09-25 2017-02-28 Idea Tree, Llc Protein isolation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1748071A1 (de) * 2004-05-21 2007-01-31 Takara Bio, Inc. Verfahren zur herstellung von polypeptid

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1748071A1 (de) * 2004-05-21 2007-01-31 Takara Bio, Inc. Verfahren zur herstellung von polypeptid

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
GOMES S L ET AL: "EXPRESSION OF THE CAULOBACTER HEAT SHOCK GENE DNA-K IS DEVELOPMENTALLY CONTROLLED DURING GROWTH AT NORMAL TEMPERATURES", JOURNAL OF BACTERIOLOGY, vol. 172, no. 6, 1990, pages 3051 - 3059, XP002463586, ISSN: 0021-9193 *
IIZUMI TARO ET AL: "Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 5, 1997, pages 1777 - 1784, XP002463552, ISSN: 0099-2240 *
RUEDIGER S ET AL: "Modulation of substrate specificity of the DnaK chaperone by alteration of a hydrophobic arch", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 304, no. 3, 2000, pages 245 - 251, XP002227984, ISSN: 0022-2836 *
RUENGELING ELKE ET AL: "Functional characterisation of the chaperones DnaK, DnaJ and GrpE from Clostridium acetobutylicum", FEMS MICROBIOLOGY LETTERS, vol. 170, no. 1, 1 January 1999 (1999-01-01), pages 119 - 123, XP002463549, ISSN: 0378-1097 *
SCHIENE-FISCHER C ET AL: "The hsp70 chaperone DnaK is a secondary amide peptide bond cis-trans isomerase", NATURE STRUCTURAL BIOLOGY, NEW YORK, NY, US, vol. 9, no. 6, 20 May 2002 (2002-05-20), pages 419 - 424, XP001156574, ISSN: 1072-8368 *
VENKATESH BALAN ET AL: "GroEL chaperone binding to beetle luciferases and the implications for refolding when co-expressed", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 68, no. 10, October 2004 (2004-10-01), pages 2096 - 2103, XP002463554, ISSN: 0916-8451 *
WETZSTEIN M ET AL: "CLONING SEQUENCING AND MOLECULAR ANALYSIS OF THE DNAK LOCUS FROM BACILLUS-SUBTILIS", JOURNAL OF BACTERIOLOGY, vol. 174, no. 10, 1992, pages 3300 - 3310, XP002463551, ISSN: 0021-9193 *

Also Published As

Publication number Publication date
DE102006028076A1 (de) 2007-12-20
WO2007147527A2 (de) 2007-12-27

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