WO2007146003A3 - Methods for ester detoxication - Google Patents

Methods for ester detoxication Download PDF

Info

Publication number
WO2007146003A3
WO2007146003A3 PCT/US2007/013333 US2007013333W WO2007146003A3 WO 2007146003 A3 WO2007146003 A3 WO 2007146003A3 US 2007013333 W US2007013333 W US 2007013333W WO 2007146003 A3 WO2007146003 A3 WO 2007146003A3
Authority
WO
WIPO (PCT)
Prior art keywords
detoxication
ester
methods
analogs
agents
Prior art date
Application number
PCT/US2007/013333
Other languages
French (fr)
Other versions
WO2007146003A2 (en
Inventor
John R Cashman
Jun Zhang
Original Assignee
Human Biomolecular Res Inst
John R Cashman
Jun Zhang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Human Biomolecular Res Inst, John R Cashman, Jun Zhang filed Critical Human Biomolecular Res Inst
Priority to JP2009514357A priority Critical patent/JP2009539376A/en
Priority to AU2007258665A priority patent/AU2007258665A1/en
Priority to CA002659097A priority patent/CA2659097A1/en
Priority to EP07809361A priority patent/EP2035032A2/en
Publication of WO2007146003A2 publication Critical patent/WO2007146003A2/en
Publication of WO2007146003A3 publication Critical patent/WO2007146003A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01008—Cholinesterase (3.1.1.8), i.e. butyrylcholine-esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

Abstract

This invention relates to a method for detoxication of inorganic or organic esters including OP nerve agents, cocaine, and respective analogs. More specifically, this invention pertains to the treatment of potentially neurotoxic esters or other ester groups by elaborating a more effective hydrolytic enzyme for therapeutic application. The structures of the synthesized OP analogs are provided. This invention also provides a diagnostic method and an Array Biosensor for detecting OP agents in biological and environmental samples.
PCT/US2007/013333 2006-06-07 2007-06-05 Methods for ester detoxication WO2007146003A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2009514357A JP2009539376A (en) 2006-06-07 2007-06-05 A new method for ester detoxification.
AU2007258665A AU2007258665A1 (en) 2006-06-07 2007-06-05 Methods for ester detoxication
CA002659097A CA2659097A1 (en) 2006-06-07 2007-06-05 Novel methods for ester detoxication
EP07809361A EP2035032A2 (en) 2006-06-07 2007-06-05 Methods for ester detoxication

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81137006P 2006-06-07 2006-06-07
US60/811,370 2006-06-07

Publications (2)

Publication Number Publication Date
WO2007146003A2 WO2007146003A2 (en) 2007-12-21
WO2007146003A3 true WO2007146003A3 (en) 2008-10-16

Family

ID=38832354

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/013333 WO2007146003A2 (en) 2006-06-07 2007-06-05 Methods for ester detoxication

Country Status (6)

Country Link
EP (1) EP2035032A2 (en)
JP (1) JP2009539376A (en)
CN (1) CN101500600A (en)
AU (1) AU2007258665A1 (en)
CA (1) CA2659097A1 (en)
WO (1) WO2007146003A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103966245B (en) * 2013-02-04 2017-06-16 南京杰蒙生物技术有限公司 The method for recombinating hBCHE in transgenic animals production using gene knock-in and nuclear transfer technology

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001625A (en) * 1995-05-19 1999-12-14 The United States Of America As Represented By The Secretary Of The Army Site-directed mutagenesis of esterases
WO2002064796A2 (en) * 2000-12-26 2002-08-22 Applied Molecular Evolution, Inc. Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use
WO2003054182A2 (en) * 2001-12-21 2003-07-03 Nexia Biotechnologies, Inc. Production of butyrylcholinesterases in transgenic mammals

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001625A (en) * 1995-05-19 1999-12-14 The United States Of America As Represented By The Secretary Of The Army Site-directed mutagenesis of esterases
WO2002064796A2 (en) * 2000-12-26 2002-08-22 Applied Molecular Evolution, Inc. Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use
WO2003054182A2 (en) * 2001-12-21 2003-07-03 Nexia Biotechnologies, Inc. Production of butyrylcholinesterases in transgenic mammals

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LYNCH T J ET AL: "Cocaine detoxification by human plasma butyrylcholinesterase.", TOXICOLOGY AND APPLIED PHARMACOLOGY AUG 1997, vol. 145, no. 2, August 1997 (1997-08-01), pages 363 - 371, XP002476585, ISSN: 0041-008X *
SUN HONG ET AL: "Cocaine metabolism accelerated by a re-engineered human butyrylcholinesterase.", THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS AUG 2002, vol. 302, no. 2, August 2002 (2002-08-01), pages 710 - 716, XP002476584, ISSN: 0022-3565 *
XIE WEIHUA ET AL: "An improved cocaine hydrolase: The A328Y mutant of human butyrylcholinesterase is 4-fold more efficient", MOLECULAR PHARMACOLOGY, BALTIMORE, MD, US, vol. 55, no. 1, January 1999 (1999-01-01), pages 83 - 91, XP002236142, ISSN: 0026-895X *

Also Published As

Publication number Publication date
EP2035032A2 (en) 2009-03-18
AU2007258665A1 (en) 2007-12-21
CA2659097A1 (en) 2007-12-21
JP2009539376A (en) 2009-11-19
CN101500600A (en) 2009-08-05
WO2007146003A2 (en) 2007-12-21

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