WO2007141580A3 - Rnase h2 complex and genes therefor - Google Patents

Rnase h2 complex and genes therefor Download PDF

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Publication number
WO2007141580A3
WO2007141580A3 PCT/GB2007/050331 GB2007050331W WO2007141580A3 WO 2007141580 A3 WO2007141580 A3 WO 2007141580A3 GB 2007050331 W GB2007050331 W GB 2007050331W WO 2007141580 A3 WO2007141580 A3 WO 2007141580A3
Authority
WO
WIPO (PCT)
Prior art keywords
rnase
genes
complex
genes therefor
therefor
Prior art date
Application number
PCT/GB2007/050331
Other languages
French (fr)
Other versions
WO2007141580A2 (en
Inventor
Andrew Peter Jackson
Yanick Joseph Crow
Christopher Paul Ponting
Original Assignee
Medical Res Council
Univ Leeds
Andrew Peter Jackson
Yanick Joseph Crow
Christopher Paul Ponting
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council, Univ Leeds, Andrew Peter Jackson, Yanick Joseph Crow, Christopher Paul Ponting filed Critical Medical Res Council
Priority to JP2009513774A priority Critical patent/JP2010528582A/en
Priority to EP07733752A priority patent/EP2029735A2/en
Publication of WO2007141580A2 publication Critical patent/WO2007141580A2/en
Publication of WO2007141580A3 publication Critical patent/WO2007141580A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

There is provided the genes for components of RNase H2, namely RNASEH2A, RNASEH2B and RNASEH2C, together with the proteins encoded thereby. Recombinant polynucleotides comprising these genes are described, optionally as vectors. Recombinant RNase H2 is also described together with an assay to assess the effect on activity of a test substance or of a modified (ie. mutated) component of the RNase H2.
PCT/GB2007/050331 2006-06-09 2007-06-11 Rnase h2 complex and genes therefor WO2007141580A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2009513774A JP2010528582A (en) 2006-06-09 2007-06-11 RNase H2 complex and gene thereof
EP07733752A EP2029735A2 (en) 2006-06-09 2007-06-11 Rnase h2 complex and genes therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0611444A GB0611444D0 (en) 2006-06-09 2006-06-09 Rnase H2 complex and genes therefor
GB0611444.1 2006-06-09

Publications (2)

Publication Number Publication Date
WO2007141580A2 WO2007141580A2 (en) 2007-12-13
WO2007141580A3 true WO2007141580A3 (en) 2008-05-02

Family

ID=36745610

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/050331 WO2007141580A2 (en) 2006-06-09 2007-06-11 Rnase h2 complex and genes therefor

Country Status (5)

Country Link
EP (1) EP2029735A2 (en)
JP (1) JP2010528582A (en)
CN (1) CN101573443A (en)
GB (1) GB0611444D0 (en)
WO (1) WO2007141580A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5539325B2 (en) 2008-04-30 2014-07-02 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド RNase H based assay using modified RNA monomers
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
JP5726729B2 (en) * 2008-06-25 2015-06-03 インスティチュート・パスツール・コリアＩｎｓｔｉｔｕｔ Ｐａｓｔｅｕｒ Ｋｏｒｅａ Genome-wide visual identification of human cofactors for HIV-1 infection
LT3460056T (en) * 2009-11-02 2020-12-28 University Of Washington Therapeutic nuclease compositions and methods
KR102596953B1 (en) 2011-04-29 2023-11-02 유니버시티 오브 워싱톤 스루 이츠 센터 포 커머셜리제이션 Therapeutic nuclease compositions and methods
DK3063275T3 (en) 2013-10-31 2019-11-25 Resolve Therapeutics Llc THERAPEUTIC NUCLEASE ALBUMIN MERGERS AND PROCEDURES
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
BR112018011503A2 (en) 2015-12-07 2018-12-04 Zymergen Inc corynebacterium glutamicum promoters
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
JP2019519241A (en) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド Method for producing a glucose permease library and its use
WO2018005655A2 (en) 2016-06-30 2018-01-04 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
JP2019519243A (en) 2016-07-05 2019-07-11 ザイマージェン インコーポレイテッド Genetic disruption of the RNA degradosome protein complex
US20210180034A1 (en) * 2018-04-10 2021-06-17 Greenlight Biosciences, Inc. T7 rna polymerase variants
CN111893186A (en) * 2020-08-20 2020-11-06 南通大学 Application of RNASEH2A inhibitor in preparation of medicine for treating liver cancer
CN114277016B (en) * 2021-12-20 2023-08-29 南京诺唯赞生物科技股份有限公司 RNase H mutant and application thereof
CN116496379B (en) * 2023-03-28 2023-12-12 新镁(上海)生物技术有限公司 Thermal stability Rnase inhiba mutant, preparation method and application

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999007891A1 (en) * 1997-08-05 1999-02-18 Human Genome Sciences, Inc. 90 human secreted proteins
EP1074617A2 (en) * 1999-07-29 2001-02-07 Helix Research Institute Primers for synthesising full-length cDNA and their use
WO2003072035A2 (en) * 2002-02-22 2003-09-04 Genentech, Inc. Compositions and methods for the treatment of immune related diseases
US20040248145A1 (en) * 1997-12-04 2004-12-09 Crooke Stanley T. Methods of using mammalian RNase H and compositions thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999007891A1 (en) * 1997-08-05 1999-02-18 Human Genome Sciences, Inc. 90 human secreted proteins
US20040248145A1 (en) * 1997-12-04 2004-12-09 Crooke Stanley T. Methods of using mammalian RNase H and compositions thereof
EP1074617A2 (en) * 1999-07-29 2001-02-07 Helix Research Institute Primers for synthesising full-length cDNA and their use
WO2003072035A2 (en) * 2002-02-22 2003-09-04 Genentech, Inc. Compositions and methods for the treatment of immune related diseases

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
ALI M ET AL: "A second locus for Aicardi-Goutieres syndrome at chromosome 13q14-21", JOURNAL OF MEDICAL GENETICS, vol. 43, no. 5, May 2006 (2006-05-01), pages 444 - 450 URL, XP008087822, ISSN: 0022-2593(print) 1468-6244(ele *
CROW YANICK J ET AL: "Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection", NATURE GENETICS, vol. 38, no. 8, August 2006 (2006-08-01), pages 910 - 916, XP002466050, ISSN: 1061-4036 *
DATABASE EPO Proteins [online] 17 December 2003 (2003-12-17), "Sequence 13573 from Patent EP1074617.", XP002455161, retrieved from EBI accession no. EPOP:AX878668 Database accession no. AX878668 *
DATABASE Geneseq [online] 14 May 1999 (1999-05-14), "Amino acid sequence of a human secreted protein.", XP002455163, retrieved from EBI accession no. GSP:AAY10834 Database accession no. AAY10834 *
DATABASE Geneseq [online] 26 February 2004 (2004-02-26), "Novel human secreted and transmembrane protein SeqID 520.", XP002455162, retrieved from EBI accession no. GSP:ADF76845 Database accession no. ADF76845 *
DATABASE UniProt [online] 1 October 2002 (2002-10-01), "RNASEH2B protein.", XP002455159, retrieved from EBI accession no. UNIPROT:Q8N451 Database accession no. Q8N451 *
DATABASE UniProt [online] 12 April 2005 (2005-04-12), "Ribonuclease H2 subunit B (RNase H2 subunit B) (Ribonuclease HI subunit B) (Aicardi-Goutieres syndrome 2 protein) (AGS2) (Deleted in lymphocytic leukemia 8).", XP002455160, retrieved from EBI accession no. UNIPROT:Q5TBB1 Database accession no. Q5TBB1 *
JEONG HO-SANG ET AL: "RNase H2 of Saccharomyces cerevisiae is a complex of three proteins.", NUCLEIC ACIDS RESEARCH, vol. 32, no. 2, 2004, pages 407 - 414, XP002455152, ISSN: 0305-1048 *
KARASINSKA JM: "Searching for the Aicardi-Goutières syndrome genes:TREX1 and ribonuclease H2 make the cut", CLIN. GENET., vol. 70, 1 November 2006 (2006-11-01), pages 457 - 458, XP002466051 *

Also Published As

Publication number Publication date
WO2007141580A2 (en) 2007-12-13
GB0611444D0 (en) 2006-07-19
CN101573443A (en) 2009-11-04
EP2029735A2 (en) 2009-03-04
JP2010528582A (en) 2010-08-26

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