WO2007139471A3 - Method for chromatography - Google Patents

Method for chromatography Download PDF

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Publication number
WO2007139471A3
WO2007139471A3 PCT/SE2007/000514 SE2007000514W WO2007139471A3 WO 2007139471 A3 WO2007139471 A3 WO 2007139471A3 SE 2007000514 W SE2007000514 W SE 2007000514W WO 2007139471 A3 WO2007139471 A3 WO 2007139471A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
chromatography
relates
solubilising
column
Prior art date
Application number
PCT/SE2007/000514
Other languages
French (fr)
Other versions
WO2007139471A2 (en
Inventor
Bengt Bjellqvist
Original Assignee
Ge Healthcare Bio Sciences Ab
Bengt Bjellqvist
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Healthcare Bio Sciences Ab, Bengt Bjellqvist filed Critical Ge Healthcare Bio Sciences Ab
Publication of WO2007139471A2 publication Critical patent/WO2007139471A2/en
Publication of WO2007139471A3 publication Critical patent/WO2007139471A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/24—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
    • B01D15/247—Fraction collectors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3842—Micellar chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8411—Intermediate storage of effluent, including condensation on surface
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/728—Intermediate storage of effluent, including condensation on surface

Abstract

The present invention relates to a method for chromatography. More closely, the method relates to a novel way of separating proteins by chromatography with results that previously only have been achieved by electrophoresis. The invention relates to a method for chromatography of proteins, comprising the following steps: a) solubilising a protein sample in a solubilising agent; b) adsorbing solubilised proteins on a chromatographic column; c) eluting proteins from the column with a gradient of surfactant solvent; and d) collecting fractions comprising eluted proteins. The invention also relates to a kit and fraction collector for use in the method.
PCT/SE2007/000514 2006-05-31 2007-05-28 Method for chromatography WO2007139471A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0601232 2006-05-31
SE0601232-2 2006-05-31

Publications (2)

Publication Number Publication Date
WO2007139471A2 WO2007139471A2 (en) 2007-12-06
WO2007139471A3 true WO2007139471A3 (en) 2008-01-24

Family

ID=38779120

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE2007/000514 WO2007139471A2 (en) 2006-05-31 2007-05-28 Method for chromatography

Country Status (1)

Country Link
WO (1) WO2007139471A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112079681B (en) * 2020-08-21 2021-08-24 中国科学院兰州化学物理研究所 Preparation method of heptadecyl benzene and octadecyl benzene sulfonate mixture

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005000226A2 (en) * 2003-06-06 2005-01-06 Diversa Corporation Mixed bed multi-dimensional chromatography systems and methods of making and using them
EP1544209A1 (en) * 2003-12-15 2005-06-22 M-Phasys GmbH Refolded membrane protein in monodisperse form

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005000226A2 (en) * 2003-06-06 2005-01-06 Diversa Corporation Mixed bed multi-dimensional chromatography systems and methods of making and using them
EP1544209A1 (en) * 2003-12-15 2005-06-22 M-Phasys GmbH Refolded membrane protein in monodisperse form

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HORNEMAN D.A. ET AL.: "Micellar gradients in size-exclusion simulated moving bed chromatography", JOURNAL OF CHROMATOGRAPHY A, vol. 1113, 2006, pages 130 - 139, XP024967909, DOI: doi:10.1016/j.chroma.2006.01.134 *

Also Published As

Publication number Publication date
WO2007139471A2 (en) 2007-12-06

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