WO2007124115A3 - Compositions and methods for identifying inhibitors and activators of cyclic amp phosphodiesterases - Google Patents

Compositions and methods for identifying inhibitors and activators of cyclic amp phosphodiesterases Download PDF

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Publication number
WO2007124115A3
WO2007124115A3 PCT/US2007/009762 US2007009762W WO2007124115A3 WO 2007124115 A3 WO2007124115 A3 WO 2007124115A3 US 2007009762 W US2007009762 W US 2007009762W WO 2007124115 A3 WO2007124115 A3 WO 2007124115A3
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WO
WIPO (PCT)
Prior art keywords
activators
methods
compositions
cyclic amp
identifying inhibitors
Prior art date
Application number
PCT/US2007/009762
Other languages
French (fr)
Other versions
WO2007124115A2 (en
Inventor
Charles S Hoffman
Original Assignee
Trustees Boston College
Charles S Hoffman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Trustees Boston College, Charles S Hoffman filed Critical Trustees Boston College
Publication of WO2007124115A2 publication Critical patent/WO2007124115A2/en
Publication of WO2007124115A3 publication Critical patent/WO2007124115A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Recombinant fission yeast cells and methods of using them are described, which provide for identification of chemical inhibitors or activators of a target exogenous phosphodiesterase (PDE). The yeast cells can be transformed using a cDNA library to identify biological inhibitors or activators of PDEs from specific cell types.
PCT/US2007/009762 2006-04-20 2007-04-20 Compositions and methods for identifying inhibitors and activators of cyclic amp phosphodiesterases WO2007124115A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79405606P 2006-04-20 2006-04-20
US60/794,056 2006-04-20

Publications (2)

Publication Number Publication Date
WO2007124115A2 WO2007124115A2 (en) 2007-11-01
WO2007124115A3 true WO2007124115A3 (en) 2007-12-21

Family

ID=38442630

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/009762 WO2007124115A2 (en) 2006-04-20 2007-04-20 Compositions and methods for identifying inhibitors and activators of cyclic amp phosphodiesterases

Country Status (1)

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WO (1) WO2007124115A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004055206A1 (en) * 2002-12-17 2004-07-01 Aventis Pharma Deutschland Gmbh Method for generating a genetically modified organism for screening active substances
WO2005023856A2 (en) * 2003-09-05 2005-03-17 University Of Bern Cyclic nucleotide-specific phosphodiesterases from leishmania and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004055206A1 (en) * 2002-12-17 2004-07-01 Aventis Pharma Deutschland Gmbh Method for generating a genetically modified organism for screening active substances
WO2005023856A2 (en) * 2003-09-05 2005-03-17 University Of Bern Cyclic nucleotide-specific phosphodiesterases from leishmania and uses thereof

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
ALONSO GUILLERMO D ET AL: "TcPDE4, a novel membrane-associated cAMP-specific phosphodiesterase from Trypanosoma cruzi.", MOLECULAR AND BIOCHEMICAL PARASITOLOGY JAN 2006, vol. 145, no. 1, January 2006 (2006-01-01), pages 40 - 49, XP005186644, ISSN: 0166-6851 *
ATIENZA J M ET AL: "Yeast Model System for Study of Mammalian Phosphodiesterases", METHODS : A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 14, no. 1, January 1998 (1998-01-01), pages 35 - 42, XP004466609, ISSN: 1046-2023 *
D'ANGELO MAXIMILIANO A ET AL: "Identification, characterization and subcellular localization of TcPDE1, a novel cAMP-specific phosphodiesterase from Trypanosoma cruzi.", THE BIOCHEMICAL JOURNAL 15 FEB 2004, vol. 378, no. Pt 1, 15 February 2004 (2004-02-15), pages 63 - 72, XP002453423, ISSN: 1470-8728 *
GUPTA R ET AL: "An update on cyclic nucleotide phosphodiesterase (PDE) inhibitors: phosphodiesterases and drug selectivity", METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY, PROUS, BARCELONA, ES, vol. 27, no. 2, March 2005 (2005-03-01), pages 101 - 118, XP009089836, ISSN: 0379-0355 *
HOFFMAN C S ET AL: "Glucose repression of transcription of the Schizosaccharomyces pombe fbp1 gene occurs by a cAMP signaling pathway.", GENES & DEVELOPMENT APR 1991, vol. 5, no. 4, April 1991 (1991-04-01), pages 561 - 571, XP002453488, ISSN: 0890-9369 *
HOFFMAN C S ET AL: "Isolation and characterization of mutants constitutive for expression of the fbp1 gene of Schizosaccharomyces pombe.", GENETICS APR 1990, vol. 124, no. 4, April 1990 (1990-04-01), pages 807 - 816, XP002453876, ISSN: 0016-6731 *
KUNZ STEFAN ET AL: "TbPDE1, a novel class I phosphodiesterase of Trypanosoma brucei.", EUROPEAN JOURNAL OF BIOCHEMISTRY / FEBS FEB 2004, vol. 271, no. 3, February 2004 (2004-02-01), pages 637 - 647, XP002322636, ISSN: 0014-2956 *
NAULA C ET AL: "Cyclic AMP signaling in trypanosomatids.", PARASITOLOGY TODAY (PERSONAL ED.) JAN 2000, vol. 16, no. 1, January 2000 (2000-01-01), pages 35 - 38, XP004908501, ISSN: 0169-4758 *
PILLAI R ET AL: "Use of a yeast expression system for the isolation and analysis of drug-resistant mutants of a mammalian phosphodiesterase.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 15 DEC 1993, vol. 90, no. 24, 15 December 1993 (1993-12-15), pages 11970 - 11974, XP002453422, ISSN: 0027-8424 *
RASCÓN ANA ET AL: "Cloning and characterization of a cAMP-specific phosphodiesterase (TbPDE2B) from Trypanosoma brucei.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2 APR 2002, vol. 99, no. 7, 2 April 2002 (2002-04-02), pages 4714 - 4719, XP002322633, ISSN: 0027-8424 *
WANG LILI ET AL: "Schizosaccharomyces pombe adenylate cyclase suppressor mutations suggest a role for cAMP phosphodiesterase regulation in feedback control of glucose/cAMP signaling.", GENETICS DEC 2005, vol. 171, no. 4, December 2005 (2005-12-01), pages 1523 - 1533, XP002453425, ISSN: 0016-6731 *

Also Published As

Publication number Publication date
WO2007124115A2 (en) 2007-11-01

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