WO2007118892A1

WO2007118892A1 - Food supplement for reinforcing the immune system

Info

Publication number: WO2007118892A1
Application number: PCT/EP2007/053796
Authority: WO
Inventors: Dirk Weickmann
Original assignee: Patentpool Innovations Management Gmbh
Priority date: 2006-04-18
Filing date: 2007-04-18
Publication date: 2007-10-25
Also published as: DE102006017950A1; WO2007118892B1

Abstract

The invention relates to a food supplement, in particular, for reinforcing the immune system, containing in addition to usual physiologically useful food substances, at least one unpurified oral secretion obtained from particular animals of the Podarcis species and a method for production of such a food supplement.

Description

Dietary supplement to strengthen the immune system

The present invention relates to dietary supplements for strengthening the immune system.

Currently, tumors are being combated as the most dangerous and dreaded diseases of our time in a very radical and less environmentally friendly way. As simple characteristic keywords can apply here: steel, beam and chemo. This means that tumors, if reasonably achievable, are in principle cut out with the steel of a knife, burned by a wide range of irradiation, or destroyed by a so-called chemotherapy with aggressive cytostatic drugs that also attack healthy cells.

Both with normal scalpel treatments and with ionizing radiation, a spatial limitation of the operating area is not possible. It will inevitably destroy healthy body cells. The unwanted side effects of chemotherapy are well known.

In contrast, attempts have been made to make cancer therapy worthy of its name more subtle. For this purpose, the rich treasure of nature was used.

For this purpose, among other things, many, from toxic organisms isolated, highly effective substances in therapeutic doses used as drugs

Thus, from DE 199 61 141 Al a pharmaceutical active ingredient is known in which it was found that components of the spider venoms of spiders of the family Sicaridae can be used for the treatment of tumor diseases. In this case, a peptide toxin from the venom of this spider species, another antagonistically active substance obtained from the venom and / or a combination of these constituents are used medicinally in the main. The total toxicity of this type of spider, so to speak a cocktail of various substances, is not pharmaceutically useful because of its already lethal effects in small doses. It can be used for the treatment of tumor diseases as well as parallel or supportive to tumor operations and residual tumor tissue can be destroyed. During therapy, genetically modified body cells (tumor cells) can be destroyed because the active substance in question recognizes the altered surface structure of such cells and kills them without complications.

In the following years, numerous active ingredients have been developed for various types of tumors and other diseases based on biogenic toxins and other biogenic agents. For large-scale application of these agents, however, large quantities are needed, which are not feasible in view of the small amounts of raw materials that can be obtained from the individual species.

Another way to fight against the onset of cancer is to make sure that it does not come to a disease in this way possible. The best way to do this is to strengthen the immune system in the right way by choosing suitable dietary supplements.

60% of the total antibodies of the human organism are produced in the gastrointestinal tract (Scientifc Concepts of Functional Food in Europe, 1999). The pathogens that the

Defense mechanisms in the nose, mouth and pharynx and the esophagus, the stomach, the bile acid and the enzymes of the pancreas have overcome, can not settle in the healthy body anywhere, since the intestine is so heavily colonized with the body's microbial lawn that here an increase is little space.

Macrophages and natural killer cells are essential elements of the body's first line of defense, that is, the natural, antigen-unspecific, cellular defense against infection. In the healthy organism these elements have a great influence on the quantity of the subsequent, adaptive, antigen-specific defense phase. The natural immune system is always capable of providing reactivating defenses, as opposed to adaptively-acquired or genetically-mediated immune deficiency.

Even in the absence of functional T cells and B cells, the immune system can quasi post infection with, for example, facultative, intracellular bacteria, such as Listeria, monocytogenes Macrophage (cellular) activating interferon (y) produce and thereby inhibit bacterial replication. The source for this are the natural killer cells, which were activated by pathogen-stimulated macrophages. This mechanism of pathogen-activated activation of the natural, cellular defense could be confirmed with a variety of microorganisms. An interaction of macrophages and natural killer cells is probably mediated not by cytokine secretion but also by membrane-bound ligands.

Moreover, macrophages, which exist in different parts of the organism, are the phagocytes (antigen-presenting or antigen-producing) of the body's own

Defense system. However, some pathogens in these macrophages can survive and multiply in the cellular system. The killing of pathogens introduced into macrophages occurs by the formation of reactive oxygen radicals, e.g. by cytokines or their genes. Activated macrophages can also be tumoricidal.

In order to train the immune system, a constant metabolic transport from the lymphoid defense system belonging to the intestinal mucosal tissue (Mucosa) must take place in the body to activate the body's immune system. Likewise, beneficial bacteria feed the mucosa with e.g. Vitamins etc to regulate bowel activity. Thus, a regenerative immune regulation with symbiotic microorganisms and related necessary substances seems useful and effective, especially since in the human body nose, mouth, throat, small intestine, large intestine, genital and mucous membrane areas are populated with vital microorganisms. Here live 400 to 500 different species.

In the prior art compositions are already known as dietary supplements, in which an essential component is a reproducible microorganism culture.

Thus, EP 0 931 543 A1 discloses multilayer tablets which are composed of two, three or more layers, one layer containing probiotic microorganisms, while the other layers contain nutritionally valuable food ingredients, such as vitamins. Mineral substances, etc., included. These tablets are characterized by a related On the microorganisms used high stability and effectiveness over a long period of time and are therefore ideally suited for storage (abstract).

It is therefore the object of the dietary supplement according to the invention, in addition to a health-promoting content of active ingredients to provide an active ingredient that specifically strengthens the immune system. A special effectiveness is to be achieved with a weakening of the immune system by tumors.

This object is achieved by the active ingredients having the features of claims 1 to 6 and the method according to claims 7-9.

According to a first aspect, the invention relates to a composition containing at least unpurified oral secretions from animals of the genus Podarcis. Nutritionally valuable substances can be added in order to obtain a composition which can preferably be used as a dietary supplement, in particular for strengthening the immune system.

An essential aspect in obtaining the active ingredient according to the invention is that the unpurified, biogenic oral secretions of lizards of the genus Podarcis and in this case of the species muralis, sicula, melisellensis and peloponnesiaca is obtained. These types are described in more detail below:

The wall lizard Podarcis muralis belongs to the family of the real lizards. She has a black head, a slender, moderately flattened body and a long tail. The head-hull length is up to 7.5cm, the tail length is 1.5 to 2.3 times this length.

The coloring is very variable and consists on the top of the most different shades of brown. The males sometimes show a greenish pattern and at the transition between flanks and abdomen also single blue scales. The predominant features are dark tones on the flanks, which usually terminate at the back with a dark longitudinal line. The back is usually lighter and sometimes has a broken longitudinal line in the middle. In males, these longitudinal drawing patterns often also dissolve in net drawing patterns. The abdominal staining ranges in the females of cream-white to copper brown and is almost monochrome. In the male, the belly can be bright yellow to orange and is often very dark spotted to almost completely black.

The wall lizard travels from northeastern Spain to central Spain eastwards across central Europe and southern Europe and in the Balkan countries to the west coast of the Black Sea. In the north, their occurrence extends to southern England.

The so-called ruin lizard Podarcis sicula has a head-trunk length in the north of Europe to about 7 cm, in the south to about 9 cm. The length of the tail here is usually twice as long as the head-trunk length. The coloring and the respective drawing are usually very variable.

The classification of the Ruins Lizard is currently being revised. Of the many described subspecies, only the two, which are likely to persist, will be mentioned here. On the one hand Podarcis sicula sicula (Rafinesque, 1810), on the other hand

Podarcis sicula campestris (De Betta, 1857). The latter has as its real distribution area the Apeninnen peninsula. The animals of the central and southern Dalmatian coast appear entrained. In the north of Dalmatia the situation is not yet clear. This animal prefers inhabiting vegetation-rich areas, but is also found on rocks and ancient walls, often even on coastal rocks in the spray zone.

Podarcis melisellensis is also called karst lizard. In Dalmatia it prefers old ruins.

Podarcis peloponnesiaca, also called Peloponnesian Wall Lizard, has a head-hull length of just over 8 cm. The tail is about twice as long. The males are slightly larger than the females. The basic color of the back is brownish, in the males also greenish. The females are colored very contrasty with four bright vertical stripes. The males have between the longitudinal stripes black blemishes. This species is endemic to the Peloponnese Peninsula in Greece and inhabits the region to about 1600 m above sea level. It has different habitats such as roadsides, olive groves or ruins. She does not like to climb and usually stays on the ground. The base material for the active ingredient according to the invention can be obtained in the following lizard species in the following manner, the application being for right-handed people:

a) Take the respective lizard in the left hand at an ambient temperature of 22 to 26 ° C. b) Approach the lizard with a sterile cotton swab and wait for the animal to bite into the swab. c) Place the respective cotton swab for about 2 hours in approximately 1 ml ultrapure water for analysis. d) Allow the water to evaporate under sterile conditions

The resulting drug is incubated in normal cell culture flasks in the presence of normal DMEM / Ham's F12 medium at 37 ° C and grows rapidly as a result. These are apparently microorganisms, which consist essentially of protein, so prions, because their growth is recorded in both aerobic and anaerobic atmosphere. This growth of the microorganisms ensures, in principle, a supply of even larger amounts of active ingredient.

A pharmaceutically effective amount of this drug is then heated to destroy any toxic components and processed in saline.

To prepare a composition according to the invention in the form of granules, the microorganisms obtained are freeze-dried to minus 21 ° C, then placed in a vacuum and further sublimated to + 4 ° C.

The mass thus obtained is supplemented with tablet filler and pressed to obtain granules. As a rule, a maximum of 10% is required. The granules thus obtained can then, depending on the desired administration form compressed into tablets or processed into capsules. In this context reference is made to the corresponding tools of galenics. Advantageously, the granules thus obtained are processed with nutritionally valuable substances into a capsule. This is also an advantage in the production of the capsules, since parallel production processes are easier to control and monitor.

As additional constituents of the dietary supplement according to the invention are vitamins, such. Beta carotene, vitamin C and / or the vitamin B complex into consideration. Furthermore, reducing sugar alcohols (sorbitol, mannitol) or oxidation-sensitive enzymes or coenzymes, as well as physiologically active plant extracts can be used.

As carrier material or as a basis for the capsules, the usual substances for tablets and dragees are used. Since the food supplement according to the invention can be used in different countries, the respective foodstuffs regulations must be observed.

These may be various forms of starch (e.g., corn starch), talc, microcrystalline cellulose, lactose, fumed silica, polyvinylpyrrolidone or cellulose powder.

Carbohydrates such as mannitol, sorbitol, xylitol, glucose, sucrose, fructose, maltose, dextrose, maltodextrin, kaolin or cellulose derivatives, such as, for example, can also be used as further constituents of the capsule preparation (for example binders and release agents). Methylcellulose or hydroxypropylcellulose, as well as calcium carbonate, calcium, magnesium or glycerol stearate and also dyes and flavorings.

The proportionate composition of these basic materials depends on the one hand on the desired content of actual active substances, such as microorganisms, vitamins, enzymes, fiber, etc., as well as on the other hand criteria that determine the mechanical-physical properties of the capsules or their components. As an example, only the hardness, the compressibility, the size and the form are mentioned here.

Claims

claims

1. Dietary supplement, in particular for strengthening the immune system, containing in addition to conventional nutritionally valuable substances at least unpurified oral secretions, obtained from animals of the genus Podarcis.

2. Dietary supplement according to claim 1, characterized in that the animals of the genus Podarcis are selected from the species Podarcis muralis, Podarcis sicula, Podarcis melisellensis and Podarcis peloponnesϊaca.

3. dietary supplement according to claim 1 or 2, characterized in that as a nutritional substance, a vitamin, preferably beta-carotene, vitamin C and / or the vitamin B complex, is used.

4. dietary supplement according to one or more of the preceding claims, characterized in that as nutritional substance, a sugar alcohol, preferably sorbitol and / or mannitol, is used.

5. Dietary supplement according to one or more of the preceding claims, characterized in that the nutritional physiological substance used is an enzyme and / or a co-enzyme, preferably an oxidation-sensitive enzyme and / or coenzyme.

6. Dietary supplement according to one or more of the preceding claims, characterized in that as a nutritional substance is an effective

Plant extract is used.

A process for the preparation of a nutritional supplement according to one or more of the preceding claims, comprising the steps of: - obtaining a oral secretion from an animal of the genus Podarcis;

Incubation of the acquired oral secretion; Concentration of the incubated oral secretion; Heating the constricted oral secretion; Displacement of the constricted oral secretion with nutritional substances.

8. The method according to claim 7, characterized in that the incubation is carried out in DMEM / Ham's F12 medium at 37 ° C.

9. The method according to claim 7 or 8, characterized in that the heated oral secretion is freeze-dried, preferably at -21 ⁰ C, and then sublimed, preferably at +4 ⁰ C.