WO2007116323A2 - Novel fertiliser combination and method for treatment of seeds and plants therewith - Google Patents
Novel fertiliser combination and method for treatment of seeds and plants therewith Download PDFInfo
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- WO2007116323A2 WO2007116323A2 PCT/IB2007/002082 IB2007002082W WO2007116323A2 WO 2007116323 A2 WO2007116323 A2 WO 2007116323A2 IB 2007002082 W IB2007002082 W IB 2007002082W WO 2007116323 A2 WO2007116323 A2 WO 2007116323A2
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- rhizobium
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
Definitions
- the invention relates to a novel fertilizer combination and to a method of treating seeds and plants with it.
- fertilizer preparations from bacteria are known as well as their application on seeds and / or crop plants such as legumes, wheat, oats, maize and cotton, for example.
- Another object of the invention is to provide a fertilizer combination whose active product is capable of penetrating into the roots of agricultural crops and of constituting nitrogen-fixing nodules on cultivated plants.
- a new fertilizing combination of the genus comprising, as active product, a bacterial combination, which combination is characterized, according to the present invention, by the fact that this bacterial association is mixed with zeolite.
- the bacterial association comprises at least one Rhizohium genus and one Bradyrhizobium genus.
- the bacterial association comprises at least Rhizobium leguminosarum, Rhizobium meliloti and Bradyrhizobium japonicum.
- the present invention also relates to a method of treating seeds and plants with a fertilizing combination whose active product is a bacterial combination, which process is characterized by the fact that this bacterial association is mixed with zeolite.
- the bacterial association comprises at least one Rhizobium genus and one Bradyrhizobium genus.
- the bacterial association comprises at least Rhizobium leguminosarum, Rhizobium meliloti and Bradyrhizobium japonicum.
- the plants are treated with the fertilizing combination so that the bacterial association produces from 0.5 to 1 million spheroplasts per plant.
- spheroplasts are obtained using lysozyme in TRIS-HCl buffer by exposure for at least 10 to 12 hours at a temperature of 37 ° C. inoculation to germinating seeds or to plant shoots using the suspension of spheroplasts obtained, followed by the growth of the plant and obtaining the pure culture of the strain of Rhizobium and Bradyrhizobium bacteria from nodules formed on the roots of plants.
- the bacterial combination is a combination fertilizer or fertilizer bacteria that improves the nitrogen nutrition of plants.
- this new fertilizing combination is based on the use of nodule activity of bacteria with the ability to fix nitrogen from the air in symbiosis with plants. Thanks to this, the soil is enriched in nitrogen.
- the bacterial association is used to increase the harvest of different agricultural crops. Crop treatment increases the crop from 1.6 to 2.0 quintals per hectare and annual and multi-year crops from 8 to 10 quintals per hectare.
- the spheroplasts were obtained from the nodule-forming strains of bacteria, preserved in the collection of the Institute of Microbiology of the National Academy of Sciences of the Republic of Armenia: Rhizobium leguminosarum INMIA B-5609; Rhizobium meliloti INMIA-5521 (mutated strain), as well as strains of bacteria preserved at the Institute of Agricultural Microbiology of Russia: Bradyrhizobium japonicum 649.
- the bacteria in the form of cultures of 24 to 48 hours are placed in a solution of lysozyme in TRIS-HCI buffer at pH 8.1.
- the incubation in lysozyme is carried out at a temperature of about 37 ° C for at least 10 to 12 hours.
- the spheroplasts are then washed from the incubation solution with sterile water and then suspended in water.
- the argumentation of quantity Optimal spheroplast counting is given in Table 1.
- the amount of spheroplasts in the suspension is defined by the direct cell calculation method under a microscope.
- the amount of spheroplasts from 0.5 to 1 million per plant is optimal, because then the formation of nodules on plant roots is the best or equal to the highest dose of spheroplasts.
- the strains passed through the various phases of the treatment are similar to the initials, those whose spheroplasts were obtained, but after their passage through cereals, some of their properties are modified. All strains studied after passage belong to Gram-negative bacteria, they are rod-shaped, as are the initial strains.
- the initial strains of Rhizobia 5609 and 5521 and the strains obtained after passage grow on the agar-agar, in the medium without Ashby nitrogen. They do not coagulate the milk and do not liquefy the gelatin.
- All wheat strains studied are not Gram-colored, that is, Gram-negative bacteria, as well as the original Rhizobium strains 5609 and 5521 that were used to obtain Gram-negative bacteria.
- spheroplasts for inoculation with wheat and oats The strains have rod-shaped cells, but smaller in size than the original cultures (see Table 2). Restaurant the cell membrane, spheroplasts are also smaller cells than those of the initial culture.
- strains 5609 and 5521 grow on the agar-agar and medium without Ashby nitrogen. They do not coagulate the milk and do not liquefy the gelatin. In comparison with the initial strains, the strains after passage I and III grow more weakly. Their colonies are bigger. Strain 5521, which is nodules on cereals after passages I and III, is similar to the parent strain from which mutated strain 5521 is derived.
- Nitragin is ecologically pure, absolutely harmless for humans and animals, it increases the yield of a number of agricultural crops (soy, tobacco, cereals, some tubers) by 15 to 40 percent.
- An absolutely new method of preparing and using a fertilizer the principle of which is the manufacture of a mixture of a bacterial association with zeolite, which is a complex of aluminum silicates, (of general formula: Me 2ZnAl 2 O 3 XSiO 2 yH 2 O, where M is a metal cation and n valence), which contain in their composition alkali metal oxides and the like, and are distinguished by an extremely regular structure of pores that are filled with water under ordinary conditions of temperature.
- Oxide content (as a percentage of weight)
- Biotite + volcanic glass 1-6 Composition of the elementary cell (coefficients of formula) of natural zeolite
- the use of the aforementioned fertilizer offers great advantages.
- the zeolite retains the soil structure, which promotes permanent aeration; it retains moisture and, most importantly, contributes to the measured use of the biological preparation, and prolongs the time of its action and its reactivation.
- Another important capacity of zeolite is that it significantly increases the resistance of seeds to any kind of diseases. According to data from American, Japanese and Armenian researchers, the use of zeolite increases the yield of agricultural crops by 10 to 12%, whereas when mixed with the bacterial combination of the invention, the effect is improved by less 2 to 2.5 times depending on the crop.
- Table 3 summarizes the results of an experiment conducted in 2002 on a four-hectare field of winter wheat.
- the activity and the radioactive element composition of the zeolite sample taken from the Noyembérian deposit (Armenia) are determined on a Ge-Li semiconductor detector low-phonic gamma-spectrometric system and support "ASPECT" program. for the treatment of spectra. Measurements (relative measurement error: 6.9%) were made on a 170-gram sample at the Radiation Atomic Control Laboratory of the Armenian Atomic Power Plant. The results of the analysis showed that the sample contains natural radioactive elements of the type uranium-238 ( 238 U) and thorium-232 ( 232 Th), as well as the radioactive element Potassium-40 ( 40 K ). Table 5 summarizes the results of the above-mentioned sample analysis:
Abstract
The novel fertiliser combination comprises the following active products, a bacterial association mixed with zeolite.
Description
NOUVELLE COMBINAISON FERTILISANTE ET PROCEDE DE TRAITEMENT PAR NEW FERTILIZER COMBINATION AND METHOD OF TREATMENT THEREOF
CELLE-CI DE GRAINES ET PLANTESTHIS OF SEEDS AND PLANTS
L'invention a trait à une nouvelle combinaison fertilisante ainsi qu'à un procédé de traitement par celle-ci de graines et de plantes.The invention relates to a novel fertilizer combination and to a method of treating seeds and plants with it.
Actuellement, on connaît des préparations fertilisantes à partir de bactéries ainsi que leur application sur de graines et/ou des plantes de culture telles que les légumineuses, le blé, l'avoine, le maïs et le coton, par exemple.Currently, fertilizer preparations from bacteria are known as well as their application on seeds and / or crop plants such as legumes, wheat, oats, maize and cotton, for example.
Mais ces combinaisons bactériologiques de l'art antérieur telles que celles commercialisées sous la marque française « Nitragin Bonus », ne donnent pas pleinement satisfaction.But these bacteriological combinations of the prior art such as those marketed under the French brand "Nitragin Bonus" do not give full satisfaction.
Aussi un des buts de la présente invention est-il de fournir une nouvelle combinaison fertilisante qui permet de surmonter les problèmes rencontrés avec les produits fertilisants actuels et notamment avec les préparations fertilisantes à partir de bactéries.It is also an object of the present invention to provide a novel fertilizer combination which makes it possible to overcome the problems encountered with current fertilizer products and in particular with fertilizer preparations from bacteria.
Un autre but de l'invention est de fournir une combinaison fertilisante dont le produit actif est capable de pénétrer dans les racines des cultures agricoles et de constituer sur les plantes cultivées des nodosités fixatrices d'azote.Another object of the invention is to provide a fertilizer combination whose active product is capable of penetrating into the roots of agricultural crops and of constituting nitrogen-fixing nodules on cultivated plants.
Ces buts, ainsi que d'autres qui apparaîtront par la suite, sont atteints par une nouvelle combinaison fertilisante du genre comprenant, comme produit actif, une association bactérienne, laquelle combinaison est caractérisée, selon la présente invention, par le fait que cette association bactérienne est mélangée avec de la zéolite.These and other objects which will become apparent are attained by a new fertilizing combination of the genus comprising, as active product, a bacterial combination, which combination is characterized, according to the present invention, by the fact that this bacterial association is mixed with zeolite.
De préférence, l'association bactérienne comprend au moins un genre Rhizohium et un genre Bradyrhizobium.Preferably, the bacterial association comprises at least one Rhizohium genus and one Bradyrhizobium genus.
Avantageusement, l'association bactérienne comprend au moins Rhizobium leguminosarum, Rhizobium meliloti et Bradyrhizobium japonicum. La présente invention concerne aussi un procédé de traitement de graines et de plantes avec une combinaison fertilisante dont le produit actif est une association bactérienne, lequel procédé est caractérisé par le fait que cette association bactérienne est mélangée avec de la zéolite.Advantageously, the bacterial association comprises at least Rhizobium leguminosarum, Rhizobium meliloti and Bradyrhizobium japonicum. The present invention also relates to a method of treating seeds and plants with a fertilizing combination whose active product is a bacterial combination, which process is characterized by the fact that this bacterial association is mixed with zeolite.
De préférence, l'association bactérienne comprend au moins un genre Rhizobium et un genre Bradyrhizobium.Preferably, the bacterial association comprises at least one Rhizobium genus and one Bradyrhizobium genus.
Avantageusement, l'association bactérienne comprend au moins Rhizobium leguminosarum, Rhizobium meliloti et Bradyrhizobium japonicum.
De préférence, les plantes sont traitées avec la combinaison fertilisante de telle sorte que l'association bactérienne produise de 0,5 à 1 million de sphéroplastes par plante.Advantageously, the bacterial association comprises at least Rhizobium leguminosarum, Rhizobium meliloti and Bradyrhizobium japonicum. Preferably, the plants are treated with the fertilizing combination so that the bacterial association produces from 0.5 to 1 million spheroplasts per plant.
La description qui va suivre et qui ne présente aucun caractère limitatif, permettra à l'homme du métier de mieux comprendre la mise en œuvre de la présente invention ainsi que les différents avantages de celle-ci.The following description, which is not limiting in nature, will enable those skilled in the art to better understand the implementation of the present invention as well as the various advantages thereof.
A partir des souches de bactéries formant des nodosités des genres Rhizobium et Bradyrhizobium, on obtient des sphéroplastes à l'aide de lysozyme dans le tampon TRIS-HCI par exposition d'au moins 10 à 12 heures à une température de 370C, l'inoculation aux graines en germination ou aux pousses des plantes à l'aide de la suspension des sphéroplastes obtenus, suivi de la croissance de la plante et de l'obtention de la culture pure de la souche des bactéries de Rhizobium et de Bradyrhizobium à partir des nodosités formées sur les racines des plantes. Mélangée à la zéolite, l'association bactérienne constitue une combinaison fertilisante ou engrais à bactéries qui améliore la nutrition en azote des plantes. L'action de cette nouvelle combinaison fertilisante est basée sur l'utilisation de l'activité des nodosités de bactéries ayant la capacité de fixer l'azote de l'air en symbiose avec les plantes. Grâce à cela, le sol s'enrichit en azote. On utilise l'association bactérienne pour augmenter la récolte de différentes cultures agricoles. Le traitement des cultures fait croître la récolte de 1 ,6 à 2,0 quintaux par hectare et celui des cultures annuelles et pluriannuelles de 8 à 10 quintaux par hectare.From the rhizobium and Bradyrhizobium nodosities forming nodule strains, spheroplasts are obtained using lysozyme in TRIS-HCl buffer by exposure for at least 10 to 12 hours at a temperature of 37 ° C. inoculation to germinating seeds or to plant shoots using the suspension of spheroplasts obtained, followed by the growth of the plant and obtaining the pure culture of the strain of Rhizobium and Bradyrhizobium bacteria from nodules formed on the roots of plants. Mixed with zeolite, the bacterial combination is a combination fertilizer or fertilizer bacteria that improves the nitrogen nutrition of plants. The action of this new fertilizing combination is based on the use of nodule activity of bacteria with the ability to fix nitrogen from the air in symbiosis with plants. Thanks to this, the soil is enriched in nitrogen. The bacterial association is used to increase the harvest of different agricultural crops. Crop treatment increases the crop from 1.6 to 2.0 quintals per hectare and annual and multi-year crops from 8 to 10 quintals per hectare.
Les sphéroplastes ont été obtenus à partir des souches de bactéries formant des nodosités, conservées dans la collection de l'Institut de microbiologie de l'Académie Nationale des Sciences de la République d'Arménie : Rhizobium leguminosarum INMIA B-5609 ; Rhizobium meliloti INMIA-5521 (souche mutée), ainsi que des souches de bactéries conservées à l'Institut de microbiologie agricole de Russie : Bradyrhizobium japonicum 649. Les bactéries sous forme de cultures de 24 à 48 heures sont placées dans une solution de lysozyme dans le tampon TRIS-HCI à pH 8,1. L'incubation dans le lysozyme est effectuée à une température d'environ 37°C pendant au moins 10 à 12 heures. Les sphéroplastes sont ensuite lavés de la solution d'incubation avec de l'eau stérile, puis mis en suspension dans de l'eau. L'argumentation de la quantité
optimale de sphéroplastes est donnée dans le Tableau 1. La quantité de sphéroplastes dans la suspension est définie par la méthode du calcul direct des cellules sous microscope.The spheroplasts were obtained from the nodule-forming strains of bacteria, preserved in the collection of the Institute of Microbiology of the National Academy of Sciences of the Republic of Armenia: Rhizobium leguminosarum INMIA B-5609; Rhizobium meliloti INMIA-5521 (mutated strain), as well as strains of bacteria preserved at the Institute of Agricultural Microbiology of Russia: Bradyrhizobium japonicum 649. The bacteria in the form of cultures of 24 to 48 hours are placed in a solution of lysozyme in TRIS-HCI buffer at pH 8.1. The incubation in lysozyme is carried out at a temperature of about 37 ° C for at least 10 to 12 hours. The spheroplasts are then washed from the incubation solution with sterile water and then suspended in water. The argumentation of quantity Optimal spheroplast counting is given in Table 1. The amount of spheroplasts in the suspension is defined by the direct cell calculation method under a microscope.
Tableau 1Table 1
Niveau de contamination des plantes selon la dose de sphéroplastes introduits par inoculationLevel of plant contamination according to the dose of spheroplasts introduced by inoculation
D'après les résultats du Tableau 1, la quantité de sphéroplastes de 0,5 à 1 million par plante est optimale, car alors la formation de nodosités sur les racines des plantes est la meilleure ou égale à la plus haute dose de sphéroplastes.
Les souches passées par les diverses phases du traitement sont semblables aux initiales, celles dont les sphéroplastes ont été obtenus, mais après leur passage par les céréales, certaines de leurs propriétés sont modifiées. Toutes les souches étudiées après passage appartiennent aux bactéries Gram-négatives, elles ont une forme de bâtonnets, de même que les souches initiales. Les souches initiales des Rhizobiums 5609 et 5521 et les souches obtenues après passage poussent sur l'agar-agar, dans le milieu sans azote d'Ashby. Elles ne coagulent pas le lait et ne liquéfient pas la gélatine. Il y a des différences dans l'intensité de croissance des souches initiales et de celles qui sont capables de former des nodosités sur les racines des céréales et dans leur rapport avec les différentes sources de carbone. Les souches obtenues sur les céréales contaminent l'ancienne plante maîtresse. C'est la principale propriété qui confirme que les souches obtenues se rapportent au Rhizobium. Les données concernant les particularités morphologiques, culturo-physiologiques et biochimiques des souches obtenues des nodosités du blé sont regroupées dans le Tableau 2 ci-après.
According to the results of Table 1, the amount of spheroplasts from 0.5 to 1 million per plant is optimal, because then the formation of nodules on plant roots is the best or equal to the highest dose of spheroplasts. The strains passed through the various phases of the treatment are similar to the initials, those whose spheroplasts were obtained, but after their passage through cereals, some of their properties are modified. All strains studied after passage belong to Gram-negative bacteria, they are rod-shaped, as are the initial strains. The initial strains of Rhizobia 5609 and 5521 and the strains obtained after passage grow on the agar-agar, in the medium without Ashby nitrogen. They do not coagulate the milk and do not liquefy the gelatin. There are differences in the growth intensity of the initial strains and those that are able to form nodules on the roots of cereals and in their relationship to different carbon sources. Strains obtained on cereals contaminate the old master plant. This is the main property that confirms that the strains obtained relate to Rhizobium. The data concerning the morphological, culturophysiological and biochemical characteristics of the strains obtained from nodules of wheat are summarized in Table 2 below.
Tableau 2Table 2
Particularités morphologiques des souches initiales de Rhizobium et des souches obtenues des tubercules de blé après passages I etMorphological features of the original Rhizobium strains and strains obtained from wheat tubers after passage I and
Note : (+) présence de la capsule ; (1) la capsule est étroite et pas présente dans toutes les cellules
Note: (+) presence of the capsule; (1) the capsule is narrow and not present in all cells
Toutes les souches passées par le blé et étudiées ne se colorient pas au Gram, c'est-à-dire qu'elles font partie des bactéries Gram-négatives, de même que les souches initiales des Rhizobiums 5609 et 5521 qui ont servi à obtenir des sphéroplastes pour en faire l'inoculation au blé et à l'avoine. Les souches ont des cellules en forme de bâtonnets, mais de dimensions plus réduites que celles des cultures initiales (voir Tableau 2). Restaurant la membrane cellulaire, les sphéroplastes constituent également des cellules plus petites que celles de la culture initiale.All wheat strains studied are not Gram-colored, that is, Gram-negative bacteria, as well as the original Rhizobium strains 5609 and 5521 that were used to obtain Gram-negative bacteria. spheroplasts for inoculation with wheat and oats. The strains have rod-shaped cells, but smaller in size than the original cultures (see Table 2). Restaurant the cell membrane, spheroplasts are also smaller cells than those of the initial culture.
Les souches initiales 5609 et 5521 , de même que celles qui forment des nodosités sur les céréales, poussent sur l'agar-agar et sur le milieu sans azote d'Ashby. Elles ne coagulent pas le lait et ne liquéfient pas la gélatine. A rencontre des souches initiales, les souches après passage I et III croissent plus faiblement. Leurs colonies sont plus grandes. La souche 5521 , qui constitue des nodosités sur les céréales après passages I et III, est semblable à Ia souche parente dont provient la souche mutée 5521.The original strains 5609 and 5521, as well as those forming nodules on cereals, grow on the agar-agar and medium without Ashby nitrogen. They do not coagulate the milk and do not liquefy the gelatin. In comparison with the initial strains, the strains after passage I and III grow more weakly. Their colonies are bigger. Strain 5521, which is nodules on cereals after passages I and III, is similar to the parent strain from which mutated strain 5521 is derived.
L'étude de l'assimilation des hydrates de carbone et des acides organiques révèle quelques différences entre les souches initiales et celles qui forment des nodosités sur les céréales. La souche 5609 du passage III, contrairement à l'initiale, croît dans un milieu à amidon, bien que très faiblement. Des différences plus notables ont été remarquées dans l'assimilation des acides organiques. Ainsi, les bactéries capables de contaminer les céréales sont dans une grande mesure semblables aux initiales dont sont obtenus les sphéroplastes d'inoculation, mais certaines de leurs propriétés sont modifiées après leur passage par les cultures des céréales. L'association bactérienne telle que celle commercialisée sous la dénomination « Nitragin » est largement utilisée dans la pratique agricole. Pour sa production, il a été utilisé un mélange de bactéries symbiotiques et non symbiotiques fixatrices d'azote. Cette préparation a été expérimentée dans différentes conditions climatiques et sur différents sols d'Arménie, ainsi qu'au cours d'expériences sur les champs de diverses exploitations sur le territoire de l'ex- URSS, en particulier dans le Réseau géographique des expériences sur le Nitragin (Saint-Pétersbourg). Il existe de nombreux comptes-rendus des expériences. La préparation de « Nitragin » est écologiquement pure, absolument inoffensive pour l'homme et les animaux, elle augmente le rendement d'un certain nombre de cultures agricoles (soja, tabac, céréales, certains tubercules) de 15 à 40 pour cent.
II a été mis en place, selon la présente invention, un procédé absolument nouveau de préparation et d'utilisation d'un engrais dont le principe est la fabrication d'un mélange d'une association bactérienne avec de la zéolite qui est un complexe de silicates d'aluminium, (de formule générale : Me2Zn AL2O3 XSiO2 yH2O, où M est un cation de métal et n la valence), qui contiennent dans leur composition des oxydes de métaux alcalins et autres, et qui se distinguent par une structure extrêmement régulière des pores qui sont remplis d'eau dans des conditions ordinaires de température.The study of the assimilation of carbohydrates and organic acids reveals some differences between the initial strains and those which form nodules on cereals. Strain 5609 of passage III, unlike the initial, grows in a starch medium, although very weakly. More notable differences have been noted in the assimilation of organic acids. Thus, bacteria capable of contaminating cereals are to a great extent similar to the initials from which inoculum spheroplasts are obtained, but some of their properties are modified after passage through cereal crops. The bacterial association such as that sold under the name "Nitragin" is widely used in agricultural practice. For its production, a mixture of symbiotic and non-symbiotic nitrogen-fixing bacteria was used. This preparation was tested in different climatic conditions and on different soils of Armenia, as well as in field experiments of various farms in the territory of the former USSR, in particular in the Geographical Network of Experiments on Nitragin (St. Petersburg). There are many reports of experiments. The preparation of "Nitragin" is ecologically pure, absolutely harmless for humans and animals, it increases the yield of a number of agricultural crops (soy, tobacco, cereals, some tubers) by 15 to 40 percent. An absolutely new method of preparing and using a fertilizer, the principle of which is the manufacture of a mixture of a bacterial association with zeolite, which is a complex of aluminum silicates, (of general formula: Me 2ZnAl 2 O 3 XSiO 2 yH 2 O, where M is a metal cation and n valence), which contain in their composition alkali metal oxides and the like, and are distinguished by an extremely regular structure of pores that are filled with water under ordinary conditions of temperature.
Les caractéristiques physico-chimiques de la zéolite naturelle du tuf (pierre volcanique) sont indiquées ci-dessous :The physico-chemical characteristics of the natural zeolite of the tuff (volcanic rock) are indicated below:
Contenu en zéolite naturelle (en pourcentage du poids) : 60-95Content in natural zeolite (as a percentage of weight): 60-95
Contenu en oxydes (en pourcentage du poids)Oxide content (as a percentage of weight)
SiO2 : 61 ,0-71 ,0 MnO : traces-0,1 TiO2 : 0,1-0,4 Na2O : 0,9-3,4SiO 2 : 61, 0-71, 0 MnO: traces-0.1 TiO 2 : 0.1-0.4 Na 2 O: 0.9-3.4
AL2O3 : 11 ,0-14,0 K2O : 1 ,0-2,8AL 2 O 3 : 11.0-14.0 K 2 O: 1.0-2.8
Fe2O3 : 0,3-2,5 P2O5 : traces-0,15Fe 2 O 3 : 0.3-2.5 P 2 O 5 : traces-0.15
FeO :0,3-2,0 H2O : 2,0-6,0 CaO : 2,8-5,0 MgO : 0,5-2,5FeO: 0.3-2.0 H 2 O: 2.0-6.0 CaO: 2.8-5.0 MgO: 0.5-2.5
Contenu en éléments toxiques (en pourcentage du poids)Content in toxic elements (as a percentage of weight)
Limite tolérée Contenu réelTolerated limit Real content
F : jusqu'à 0,15 nulF: up to 0.15 zero
As : jusqu'à 0,01 traces Pb :jusqu'à 0,006 0,001-0,006As: up to 0.01 Pb traces: up to 0.006 0.001-0.006
Hg : jusqu'à 0,0005 tracesHg: up to 0.0005 traces
Cd : jusqu'à 0,05 tracesCd: up to 0.05 traces
Contenu en minéraux additionnels (en pourcentage du poids):Additional mineral content (as a percentage of weight):
Montmorillonite 1-20 Quartz 1-6Montmorillonite 1-20 Quartz 1-6
Calcite 1-4Calcite 1-4
Feldspath 1-4Feldspar 1-4
Biotite + verre volcanique 1-6
Composition de l'alvéole élémentaire (coefficients de formule) de zéolite naturelleBiotite + volcanic glass 1-6 Composition of the elementary cell (coefficients of formula) of natural zeolite
Si : 29,55 Na : 0,70If: 29.55 Na: 0.70
Al : 6,53 K : 1 ,22 Mg : 0,10 ' H20 : 19,6Al: 6.53 K: 1, 22 Mg: 0.10 H 2 0: 19.6
Ca : 2,20Ca: 2.20
Dimensions des pores (en angstrôms) : 4 ÂPore size (in angstroms): 4 Â
Paramètres de l'alvéole élémentaire : a = 17,63 À ; B = 17,90 Â; c = 17,90 A Porosité 24,87-32,36% moyenne - 28,17%Parameters of the elementary cell: a = 17.63 Å; B = 17.90; c = 17.90 A Porosity 24.87-32.36% average - 28.17%
Densité 2,2-2,6 g/cm3 Density 2.2-2.6 g / cm 3
Masse des terres rapportées à l'extraction 1 ,3-1 ,6 g/cm3 Masse volumique 1 ,7-1 ,0 g/cm3 Mass of the earth relative to extraction 1, 3-1, 6 g / cm 3 Density 1, 7-1, 0 g / cm 3
Limite de solidité à la pression mono-axiale 334-952 kg/cm3 Moyenne : 438 kg/cm3 Strength limit at single-axial pressure 334-952 kg / cm 3 Average: 438 kg / cm 3
Principaux cations d'échange : Ca et Na et K et Mg Quantité de cations d'échange (en mg équiv. pour 100g de roche) Ca2+ : 22,0-59,0 Mg2+ : 4,0-18,0 Na+ : 22,0-59,0Main exchange cations: Ca and Na and K and Mg Quantity of exchange cations (in mg equiv for 100g of rock) Ca 2+ : 22.0-59.0 Mg 2+ : 4.0-18, Na + : 22.0-59.0
K+ : 1 ,2-10,0 Somme (Ca2+ + Mg2+ + Na+ + K+) = 72-123K + : 1, 2-10.0 Sum (Ca 2+ + Mg 2+ + Na + + K + ) = 72-123
Rapport Na+ + K+ = 0,26-1 ,77 Ca2+ + Mg2+ Na + + K + ratio = 0.26-1, 77 Ca 2+ + Mg 2+
Résistance aux acides (pH) : de 0,4 à 7 Résistance aux bases (pH) : de 7 à 13,8Resistance to acids (pH): from 0.4 to 7 Resistance to bases (pH): from 7 to 13.8
L'utilisation de l'engrais susmentionné (association bactérienne mélangée avec de la zéolite selon la présente invention et dans une proportion déterminée) offre de grands avantages. La zéolite conserve la structure du sol, ce qui en favorise l'aération permanente ; elle retient l'humidité et, ce qui est le plus important, contribue à l'utilisation dosée de Ia préparation biologique, et prolonge les délais de son action et de sa réactivation. La zéolite possède une autre capacité importante : elle accroît sensiblement la résistance des graines à l'égard de toute
sorte de maladies. Selon les données des chercheurs américains, japonais et arméniens, l'emploi de la zéolite augmente de 10 à 12 % le rendement des cultures agricoles, alors que mélangée à l'association bactérienne de l'invention, l'effet est amélioré d'au moins 2 à 2,5 fois selon la culture.The use of the aforementioned fertilizer (bacterial combination mixed with zeolite according to the present invention and in a specific proportion) offers great advantages. The zeolite retains the soil structure, which promotes permanent aeration; it retains moisture and, most importantly, contributes to the measured use of the biological preparation, and prolongs the time of its action and its reactivation. Another important capacity of zeolite is that it significantly increases the resistance of seeds to any kind of diseases. According to data from American, Japanese and Armenian researchers, the use of zeolite increases the yield of agricultural crops by 10 to 12%, whereas when mixed with the bacterial combination of the invention, the effect is improved by less 2 to 2.5 times depending on the crop.
Dans le Tableau 3 ci-après sont regroupés les résultats d'une expérience réalisée en 2002 sur un champ de quatre hectares de blé d'automne.Table 3 below summarizes the results of an experiment conducted in 2002 on a four-hectare field of winter wheat.
Tableau 3Table 3
Les spécificités des interrelations des bactéries formant des nodosités avec les plantes cultivées à l'aide de la zéolite sont exposées dans le Tableau 4.The specificities of the interrelation of nodule-forming bacteria with plants grown with zeolite are shown in Table 4.
Tableau 4Table 4
L'activité et la composition en éléments radioactifs de l'échantillon de zéolite, prélevé du gisement de Noyembérian (Arménie) sont déterminées sur une installation gamma-spectrométrique bas-phonique à détecteur semi-conducteur Ge- Li et programme « ASPECT » de soutien pour le traitement des spectres. Les mesures (erreur relative de mesure : 6,9 %) ont été effectuées sur un échantillon de 170 grammes au laboratoire du contrôle extérieur de radiation de la Centrale Atomique Arménienne. Les résultats de l'analyse ont montré que l'échantillon contient des éléments radioactifs naturels du type d'uranium-238 (238U) et de thorium-232 (232Th), ainsi que l'élément radioactif Potassium-40 (40K). Le Tableau 5 regroupe les résultats de l'analyse de l'échantillon susmentionné :The activity and the radioactive element composition of the zeolite sample taken from the Noyembérian deposit (Armenia) are determined on a Ge-Li semiconductor detector low-phonic gamma-spectrometric system and support "ASPECT" program. for the treatment of spectra. Measurements (relative measurement error: 6.9%) were made on a 170-gram sample at the Radiation Atomic Control Laboratory of the Armenian Atomic Power Plant. The results of the analysis showed that the sample contains natural radioactive elements of the type uranium-238 ( 238 U) and thorium-232 ( 232 Th), as well as the radioactive element Potassium-40 ( 40 K ). Table 5 summarizes the results of the above-mentioned sample analysis:
Tableau 5Table 5
Ci-dessous dans le Tableau 6, sont citées pour comparaison les données de l'activité spécifique typique des éléments radioactifs dans les roches ordinaires (AA Moisséev, VA. Ivanov, Livre de Référence de dosimétrie et d'hygiène de la radiation, Moscou, « Energoatomizdat », 1984, p. 71 ).
Tableau 6In Table 6, the data for the typical specific activity of radioactive elements in ordinary rocks are given for comparison (AA Moisseev, VA Ivanov, Radiation Dosimetry and Radiation Hygiene Reference Book, Moscow, "Energoatomizdat", 1984, 71). Table 6
Claims
1. - Nouvelle combinaison fertilisante du genre comprenant, comme produit actif, une association bactérienne, caractérisée par le fait que ladite association bactérienne est mélangée avec de la zéolite.1. - New fertilizing combination of the genus comprising, as active product, a bacterial association, characterized in that said bacterial association is mixed with zeolite.
2. - Nouvelle combinaison fertilisante selon la revendication 1 , caractérisée par le fait que l'association bactérienne comprend au moins un genre Rhizobium et un genre Bradyrhizobium.2. - New combination fertilizer according to claim 1, characterized in that the bacterial association comprises at least one genus Rhizobium and a genus Bradyrhizobium.
3. - Nouvelle combinaison fertilisante selon la revendication 2, caractérisée par le fait que l'association bactérienne comprend au moins Rhizobium leguminosarum, Rhizobium meliloti et Bradyrhizobium japonicum.3. - New combination fertilizer according to claim 2, characterized in that the bacterial association comprises at least Rhizobium leguminosarum, Rhizobium meliloti and Bradyrhizobium japonicum.
4. - Procédé de traitement de graines et de plantes avec une combinaison fertilisante dont le produit actif est une association bactérienne, selon l'une quelconque des revendications 1 à 3, caractérisé par le fait que cette association bactérienne est mélangée avec de la zéolite.4. - A method of treating seeds and plants with a fertilizer combination whose active ingredient is a bacterial combination, according to any one of claims 1 to 3, characterized in that this bacterial combination is mixed with zeolite.
5. - Procédé selon la revendication 4, caractérisée par le fait que l'association bactérienne comprend au moins un genre Rhizobium et un genre5. - Method according to claim 4, characterized in that the bacterial association comprises at least one genus Rhizobium and a genus
Bradyrhizobium.Bradyrhizobium.
6. - Procédé selon la revendication 5, caractérisée par le fait que l'association bactérienne comprend au moins Rhizobium leguminosarum, Rhizobium meliloti et Bradyrhizobium japonicum.6. - Process according to claim 5, characterized in that the bacterial association comprises at least Rhizobium leguminosarum, Rhizobium meliloti and Bradyrhizobium japonicum.
7. - Procédé selon l'une queconque des revendications 4 à 6, caractérisé en ce que les plantes sont traitées avec ladite combinaison fertilisante de telle sorte que l'association bactérienne produise de 0,5 à 1 million de sphéroplastes par plante. 7. - Method according to any one of claims 4 to 6, characterized in that the plants are treated with said fertilizer combination so that the bacterial association produces from 0.5 to 1 million spheroplasts per plant.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR06.03146 | 2006-04-10 | ||
| FR0603146A FR2899580B1 (en) | 2006-04-10 | 2006-04-10 | NITRAGIN AND NATURAL ZEOLITE: NEW METHODS OF THEIR USE IN AGRICULTURE. |
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| WO2007116323A2 true WO2007116323A2 (en) | 2007-10-18 |
| WO2007116323A3 WO2007116323A3 (en) | 2008-06-12 |
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| CN (1) | CN101466652A (en) |
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| WO2017209593A1 (en) * | 2016-06-02 | 2017-12-07 | Gallegos Acuña Horacio | Soil bioregenerator |
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| CN102174436B (en) * | 2011-01-24 | 2012-06-27 | 领先生物农业股份有限公司 | Bradyrhizobium japonicum capable of effectively fixing nitrogen and culture method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1416043A1 (en) * | 2001-07-10 | 2004-05-06 | Japan Science and Technology Corporation | Leguminous bacterium having potentiated nitrogen fixation ability |
| WO2005095579A1 (en) * | 2004-03-31 | 2005-10-13 | Danisco A/S | Process |
| WO2007030557A2 (en) * | 2005-09-08 | 2007-03-15 | Cornell Research Foundation, Inc. | Formulations of viable microorganisms and their methods of production and use |
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|---|---|---|---|---|
| JPS61209981A (en) * | 1985-03-12 | 1986-09-18 | 片倉チツカリン株式会社 | Manufacture of fertilizer |
| AUPS338302A0 (en) * | 2002-07-04 | 2002-07-25 | Bayclassic Pty Ltd | Rhizobial inoculant |
-
2006
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2007
- 2007-04-10 WO PCT/IB2007/002082 patent/WO2007116323A2/en active Application Filing
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1416043A1 (en) * | 2001-07-10 | 2004-05-06 | Japan Science and Technology Corporation | Leguminous bacterium having potentiated nitrogen fixation ability |
| WO2005095579A1 (en) * | 2004-03-31 | 2005-10-13 | Danisco A/S | Process |
| WO2007030557A2 (en) * | 2005-09-08 | 2007-03-15 | Cornell Research Foundation, Inc. | Formulations of viable microorganisms and their methods of production and use |
Non-Patent Citations (2)
| Title |
|---|
| CHILD J J ET AL: "SPHEROPLASTS FROM RHIZOBIUM-JAPONICUM" PLANT SCIENCE LETTERS, ELSEVIER SCIENCE PUBLISHERS LTD, LONDON, GB, vol. 4, no. 4, avril 1975 (1975-04), pages 267-272, XP008077201 ISSN: 0304-4211 * |
| DATABASE WPI Week 198644 Derwent Publications Ltd., London, GB; AN 1986-287903 XP002471435 & JP 61 209981 A (KATAKURA CHIKKARIN CO LTD) 18 septembre 1986 (1986-09-18) * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017209593A1 (en) * | 2016-06-02 | 2017-12-07 | Gallegos Acuña Horacio | Soil bioregenerator |
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| FR2899580B1 (en) | 2008-06-06 |
| CN101466652A (en) | 2009-06-24 |
| FR2899580A1 (en) | 2007-10-12 |
| WO2007116323A3 (en) | 2008-06-12 |
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