WO2007114931A3 - Detection nanoelectronique de biomolecules en employant l'amplification des analytes et des rapporteurs - Google Patents

Detection nanoelectronique de biomolecules en employant l'amplification des analytes et des rapporteurs Download PDF

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Publication number
WO2007114931A3
WO2007114931A3 PCT/US2007/008422 US2007008422W WO2007114931A3 WO 2007114931 A3 WO2007114931 A3 WO 2007114931A3 US 2007008422 W US2007008422 W US 2007008422W WO 2007114931 A3 WO2007114931 A3 WO 2007114931A3
Authority
WO
WIPO (PCT)
Prior art keywords
reporters
detection
biomolecules
nanoelectronic
analyte amplification
Prior art date
Application number
PCT/US2007/008422
Other languages
English (en)
Other versions
WO2007114931A2 (fr
Inventor
Eugene Tu
Christian Valcke
Original Assignee
Nanomix Inc
Eugene Tu
Christian Valcke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/488,465 external-priority patent/US20070045756A1/en
Priority claimed from US11/588,845 external-priority patent/US20080021339A1/en
Application filed by Nanomix Inc, Eugene Tu, Christian Valcke filed Critical Nanomix Inc
Priority to JP2009504284A priority Critical patent/JP2009532064A/ja
Priority to EP07754869A priority patent/EP2007379A4/fr
Publication of WO2007114931A2 publication Critical patent/WO2007114931A2/fr
Publication of WO2007114931A3 publication Critical patent/WO2007114931A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs

Abstract

La présente invention concerne des procédés de détection de biomolécules, y compris des procédés d'amplification d'espèces d'analytes cibles et de rapporteurs cibles par l'action déclenchée par l'analyte d'une enzyme telle qu'une nucléase, une polymérase ou une enzyme similaire. Les espèces cibles amplifiées (par exemple des produits d'amplification et des rapporteurs) sont détectables à l'aide de plusieurs modes de réalisation de capteurs nanoélectroniques selon des aspects de l'invention et, à titre d'alternative, par des procédés conventionnels de détection des biomolécules.
PCT/US2007/008422 2006-04-04 2007-04-03 Detection nanoelectronique de biomolecules en employant l'amplification des analytes et des rapporteurs WO2007114931A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2009504284A JP2009532064A (ja) 2006-04-04 2007-04-03 被検体増幅とレポーターを用いる生体分子のナノ電子的検出
EP07754869A EP2007379A4 (fr) 2006-04-04 2007-04-03 Detection nanoelectronique de biomolecules en employant l'amplification des analytes et des rapporteurs

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US78902206P 2006-04-04 2006-04-04
US60/789,022 2006-04-04
US11/488,465 US20070045756A1 (en) 2002-09-04 2006-07-18 Nanoelectronic sensor with integral suspended micro-heater
US11/488,465 2006-07-18
US85021706P 2006-10-06 2006-10-06
US60/850,217 2006-10-06
US11/588,845 US20080021339A1 (en) 2005-10-27 2006-10-26 Anesthesia monitor, capacitance nanosensors and dynamic sensor sampling method
US11/588,845 2006-10-26
US90153807P 2007-02-14 2007-02-14
US60/901,538 2007-02-14

Publications (2)

Publication Number Publication Date
WO2007114931A2 WO2007114931A2 (fr) 2007-10-11
WO2007114931A3 true WO2007114931A3 (fr) 2008-12-24

Family

ID=38564139

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/008422 WO2007114931A2 (fr) 2006-04-04 2007-04-03 Detection nanoelectronique de biomolecules en employant l'amplification des analytes et des rapporteurs

Country Status (3)

Country Link
EP (1) EP2007379A4 (fr)
JP (1) JP2009532064A (fr)
WO (1) WO2007114931A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8152991B2 (en) 2005-10-27 2012-04-10 Nanomix, Inc. Ammonia nanosensors, and environmental control system
US8154093B2 (en) 2002-01-16 2012-04-10 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
US7522040B2 (en) 2004-04-20 2009-04-21 Nanomix, Inc. Remotely communicating, battery-powered nanostructure sensor devices
US7714398B2 (en) 2002-09-05 2010-05-11 Nanomix, Inc. Nanoelectronic measurement system for physiologic gases and improved nanosensor for carbon dioxide
US7948041B2 (en) 2005-05-19 2011-05-24 Nanomix, Inc. Sensor having a thin-film inhibition layer
EP2462051A4 (fr) 2009-08-07 2013-06-26 Nanomix Inc Biodétection à base d'un nanotube de carbone magnétique
ES2877551T3 (es) 2013-04-24 2021-11-17 Fresenius Kabi Deutschland Gmbh Procedimiento de funcionamiento de un dispositivo de control para controlar un dispositivo de infusión

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759773A (en) * 1993-01-15 1998-06-02 The Public Health Research Institute Of The City Of New York, Inc. Sensitive nucleic acid sandwich hybridization assay
US20040033525A1 (en) * 1996-12-10 2004-02-19 Monforte Joseph A. Releasable nonvolatile mass-label molecules
US20060014172A1 (en) * 2004-05-03 2006-01-19 Nanosphere, Inc. Aptamer-nanoparticle conjugates and method of use for target analyte detection
US20060035215A9 (en) * 1999-10-29 2006-02-16 Sorge Joseph A Methods for detection of a target nucleic acid by capture
US20060040294A1 (en) * 1996-01-24 2006-02-23 Prudent James R Cleavage of nucleic acids

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030036065A1 (en) * 2001-08-17 2003-02-20 Robert Gellibolian Method and system for array signal generation and amplification
WO2003089654A2 (fr) * 2002-04-22 2003-10-30 Wisconsin Alumni Research Foundation Analyse de polymorphismes mononucleotidiques a l'aide de reactions de clivage invasives de surface
US20040214176A1 (en) * 2003-04-22 2004-10-28 Osborne James C. Multiplexed DNA assays using structure-specific endonucleases
CA2545252A1 (fr) * 2003-11-06 2005-05-26 University Of Nevada, Reno Procedes ameliores pour la detection et la mesure de sequences d'acides nucleiques specifiques
JP2008511008A (ja) * 2004-08-24 2008-04-10 ナノミックス・インコーポレーテッド Ｄｎａ検出用ナノチューブセンサー装置
JP2008525822A (ja) * 2004-12-28 2008-07-17 ナノミックス・インコーポレーテッド ポリヌクレオチド配列のｄｎａ検出／認識用ナノ電子装置

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759773A (en) * 1993-01-15 1998-06-02 The Public Health Research Institute Of The City Of New York, Inc. Sensitive nucleic acid sandwich hybridization assay
US20060040294A1 (en) * 1996-01-24 2006-02-23 Prudent James R Cleavage of nucleic acids
US20040033525A1 (en) * 1996-12-10 2004-02-19 Monforte Joseph A. Releasable nonvolatile mass-label molecules
US20060035215A9 (en) * 1999-10-29 2006-02-16 Sorge Joseph A Methods for detection of a target nucleic acid by capture
US20060014172A1 (en) * 2004-05-03 2006-01-19 Nanosphere, Inc. Aptamer-nanoparticle conjugates and method of use for target analyte detection

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of EP2007379A4 *
STAR ET AL.: "Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors", PROC. NATL. ACAD. SCI. USA, vol. 103, no. 4, 24 January 2006 (2006-01-24), pages 921 - 926, XP003023787 *

Also Published As

Publication number Publication date
WO2007114931A2 (fr) 2007-10-11
EP2007379A4 (fr) 2009-12-09
EP2007379A2 (fr) 2008-12-31
JP2009532064A (ja) 2009-09-10

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