WO2007109677A3 - A novel method of protecting islet cells from apoptosis during the donor harvesting process - Google Patents

A novel method of protecting islet cells from apoptosis during the donor harvesting process Download PDF

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Publication number
WO2007109677A3
WO2007109677A3 PCT/US2007/064424 US2007064424W WO2007109677A3 WO 2007109677 A3 WO2007109677 A3 WO 2007109677A3 US 2007064424 W US2007064424 W US 2007064424W WO 2007109677 A3 WO2007109677 A3 WO 2007109677A3
Authority
WO
WIPO (PCT)
Prior art keywords
novel method
islet cells
harvesting process
apoptosis during
donor harvesting
Prior art date
Application number
PCT/US2007/064424
Other languages
French (fr)
Other versions
WO2007109677A2 (en
Inventor
John E Thompson
Charles A Dinarello
Original Assignee
Senesco Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Senesco Technologies Inc filed Critical Senesco Technologies Inc
Priority to EP07758926A priority Critical patent/EP1996707A2/en
Priority to CA002637137A priority patent/CA2637137A1/en
Priority to JP2009501701A priority patent/JP5543774B2/en
Priority to PCT/US2007/064424 priority patent/WO2007109677A2/en
Priority to AU2007226878A priority patent/AU2007226878A1/en
Priority to TW096116135A priority patent/TW200839013A/en
Priority to ARP070101971A priority patent/AR060785A1/en
Publication of WO2007109677A2 publication Critical patent/WO2007109677A2/en
Publication of WO2007109677A3 publication Critical patent/WO2007109677A3/en
Priority to IL192388A priority patent/IL192388A0/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Emergency Medicine (AREA)
  • Cell Biology (AREA)
  • Transplantation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to methods for improving the viability and recovery of islets that are separated from a donor organ for subsequent transplantation and more particularly relates to the use of eIF5A siRNAs to enhance the viability of islets.
PCT/US2007/064424 2006-03-20 2007-03-20 A novel method of protecting islet cells from apoptosis during the donor harvesting process WO2007109677A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP07758926A EP1996707A2 (en) 2006-03-20 2007-03-20 A novel method of protecting islet cells from apoptosis during the donor harvesting process
CA002637137A CA2637137A1 (en) 2006-03-20 2007-03-20 A novel method of protecting islet cells from apoptosis during the donor harvesting process
JP2009501701A JP5543774B2 (en) 2006-03-20 2007-03-20 A novel method to prevent islet cells from being apoptotic during the donor harvest process
PCT/US2007/064424 WO2007109677A2 (en) 2006-03-20 2007-03-20 A novel method of protecting islet cells from apoptosis during the donor harvesting process
AU2007226878A AU2007226878A1 (en) 2006-03-20 2007-03-20 A novel method of protecting islet cells from apoptosis during the donor harvesting process
TW096116135A TW200839013A (en) 2007-03-20 2007-05-07 A novel method of protecting islet cells from apoptosis during the donor harvesting process
ARP070101971A AR060785A1 (en) 2006-03-20 2007-05-07 METHOD FOR THE PROTECTION OF LANGERHANS ISLANDS CELLS AGAINST APOPTOSIS DURING THE PROCESS OF OBTAINING DONOR CELLS
IL192388A IL192388A0 (en) 2006-03-20 2008-06-23 A novel method of protecting islet cells from apoptosis during the donor harvesting process

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US78341406P 2006-03-20 2006-03-20
US60/783,414 2006-03-20
PCT/US2007/064424 WO2007109677A2 (en) 2006-03-20 2007-03-20 A novel method of protecting islet cells from apoptosis during the donor harvesting process

Publications (2)

Publication Number Publication Date
WO2007109677A2 WO2007109677A2 (en) 2007-09-27
WO2007109677A3 true WO2007109677A3 (en) 2008-04-24

Family

ID=39680975

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/064424 WO2007109677A2 (en) 2006-03-20 2007-03-20 A novel method of protecting islet cells from apoptosis during the donor harvesting process

Country Status (7)

Country Link
EP (1) EP1996707A2 (en)
JP (1) JP5543774B2 (en)
AR (1) AR060785A1 (en)
AU (1) AU2007226878A1 (en)
CA (1) CA2637137A1 (en)
IL (1) IL192388A0 (en)
WO (1) WO2007109677A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090093434A1 (en) * 2007-08-20 2009-04-09 Senesco Technologies, Inc. Use of eif-5a1 sirna to protect islets cells from apoptosis and to preserve their functionality

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003010286A2 (en) * 2001-07-23 2003-02-06 Senesco, Inc. Nucleic acids, polypeptides, and methods for modulating apoptosis
WO2005007853A2 (en) * 2003-06-06 2005-01-27 Senesco Technologies, Inc. Inhibition of apoptosis-specific elf-5a (“eif-5a1”) with antisense oligonucleotides and sirnas as anti-inflammatory therapeutics

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006520611A (en) * 2003-03-05 2006-09-14 セネスコ テクノロジーズ，インコーポレイティド Use of antisense oligonucleotides or siRNA to suppress the expression of eIF-5A1

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003010286A2 (en) * 2001-07-23 2003-02-06 Senesco, Inc. Nucleic acids, polypeptides, and methods for modulating apoptosis
WO2005007853A2 (en) * 2003-06-06 2005-01-27 Senesco Technologies, Inc. Inhibition of apoptosis-specific elf-5a (“eif-5a1”) with antisense oligonucleotides and sirnas as anti-inflammatory therapeutics

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BRADLEY S P ET AL: "Successful incorporation of short-interfering RNA into islet cells by in situ perfusion", TRANSPLANTATION PROCEEDINGS, ORLANDO, FL, US, vol. 37, no. 1, January 2005 (2005-01-01), pages 233 - 236, XP004819911, ISSN: 0041-1345 *
LEWIS D L ET AL: "Efficient delivery of siRNA for inhibition of gene expression in postnatal mice", NATURE GENETICS, NATURE AMERICA, NEW YORK, US, vol. 32, September 2002 (2002-09-01), pages 107 - 108, XP002978928, ISSN: 1061-4036 *
LEWIS E C ET AL: "alpha1-Antitrypsin Monotherapy Prolongs Islet Allograft Survival in Mice", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 102, no. 34, 23 August 2005 (2005-08-23), pages 12153 - 12158, XP003011675, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
JP2009530418A (en) 2009-08-27
AU2007226878A1 (en) 2007-09-27
AR060785A1 (en) 2008-07-10
JP5543774B2 (en) 2014-07-09
IL192388A0 (en) 2008-12-29
CA2637137A1 (en) 2007-09-27
EP1996707A2 (en) 2008-12-03
WO2007109677A2 (en) 2007-09-27

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