WO2007107956A3 - Method and apparatus for detecting chromosome contents in spermatozoa of domestic animals, in particular for sorting such spermatozoa - Google Patents

Method and apparatus for detecting chromosome contents in spermatozoa of domestic animals, in particular for sorting such spermatozoa Download PDF

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Publication number
WO2007107956A3
WO2007107956A3 PCT/IB2007/050973 IB2007050973W WO2007107956A3 WO 2007107956 A3 WO2007107956 A3 WO 2007107956A3 IB 2007050973 W IB2007050973 W IB 2007050973W WO 2007107956 A3 WO2007107956 A3 WO 2007107956A3
Authority
WO
WIPO (PCT)
Prior art keywords
spermatozoa
domestic animals
sorting
detecting chromosome
contents
Prior art date
Application number
PCT/IB2007/050973
Other languages
French (fr)
Other versions
WO2007107956A2 (en
Inventor
Maurizio Grego
Bruno Raso
Riccardo Aleandri
Andrea Galli
Original Assignee
Amc Italy S R L
Istituto Sperimentale Italiano
Maurizio Grego
Bruno Raso
Riccardo Aleandri
Andrea Galli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amc Italy S R L, Istituto Sperimentale Italiano, Maurizio Grego, Bruno Raso, Riccardo Aleandri, Andrea Galli filed Critical Amc Italy S R L
Publication of WO2007107956A2 publication Critical patent/WO2007107956A2/en
Publication of WO2007107956A3 publication Critical patent/WO2007107956A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/21—Polarisation-affecting properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/3581—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/85—Investigating moving fluids or granular solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Dispersion Chemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

A method and an apparatus for detecting chromosome contents in spermatozoa of domestic animals, in particular for sexing such spermatozoa, are described. For such a determination, a source (1) of electromagnetic radiations having frequencies in the Terahertz range illuminates a flow of spermatozoa (5), and detection and analysis means (6, 8) directly measure the vibrational response of DNA in a spermatozoon when the latter is illuminated by the radiation. The measure is performed by evaluating the variations of predetermined characteristics of the radiation, such as power and polarisation, induced by the interaction with the spermatozoon.
PCT/IB2007/050973 2006-03-22 2007-03-20 Method and apparatus for detecting chromosome contents in spermatozoa of domestic animals, in particular for sorting such spermatozoa WO2007107956A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITCN2006A000005 2006-03-22
IT000005A ITCN20060005A1 (en) 2006-03-22 2006-03-22 PROCEDURE AND EQUIPMENT TO DETECT THE CHROMOSOMAL CONTENT OF ANIMAL SPERMATOZOO, IN PARTICULAR FOR THE PURPOSES OF THE SESSION OF SUCH SPERMATOZOI

Publications (2)

Publication Number Publication Date
WO2007107956A2 WO2007107956A2 (en) 2007-09-27
WO2007107956A3 true WO2007107956A3 (en) 2007-12-13

Family

ID=38462313

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2007/050973 WO2007107956A2 (en) 2006-03-22 2007-03-20 Method and apparatus for detecting chromosome contents in spermatozoa of domestic animals, in particular for sorting such spermatozoa

Country Status (2)

Country Link
IT (1) ITCN20060005A1 (en)
WO (1) WO2007107956A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3132979A1 (en) * 2019-03-12 2020-09-17 Inguran, Llc Methods for sex-sorting sperm
CN115236038B (en) * 2022-07-26 2024-03-22 合肥综合性国家科学中心能源研究院(安徽省能源实验室) Terahertz imaging method for acrosome vacuoles of sperms
CN116008217B (en) * 2022-10-31 2024-01-30 合肥综合性国家科学中心能源研究院(安徽省能源实验室) Measurement method for terahertz imaging of sperm tail

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003062794A2 (en) * 2002-01-24 2003-07-31 Breslin, John Method of using electromagnetic absorption or perturbation spectra to diagnose and detect abnormalities in cells, tissues and organisms
DE10257225B3 (en) * 2002-12-07 2004-04-08 Technische Universität Braunschweig Carolo-Wilhelmina Assembly to analyze chemical/biological substances, uses terahertz spectroscopy, with an electromagnetic field in the space containing the substance between base and micro strip conductors
US20050112541A1 (en) * 2003-03-28 2005-05-26 Monsanto Technology Llc Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
JP2005172775A (en) * 2003-12-05 2005-06-30 Semiconductor Res Found Method and device for inspecting food using irradiation with electromagnetic wave

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003062794A2 (en) * 2002-01-24 2003-07-31 Breslin, John Method of using electromagnetic absorption or perturbation spectra to diagnose and detect abnormalities in cells, tissues and organisms
DE10257225B3 (en) * 2002-12-07 2004-04-08 Technische Universität Braunschweig Carolo-Wilhelmina Assembly to analyze chemical/biological substances, uses terahertz spectroscopy, with an electromagnetic field in the space containing the substance between base and micro strip conductors
US20050112541A1 (en) * 2003-03-28 2005-05-26 Monsanto Technology Llc Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
JP2005172775A (en) * 2003-12-05 2005-06-30 Semiconductor Res Found Method and device for inspecting food using irradiation with electromagnetic wave

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GLOBUS T R ET AL: "THz-spectroscopy of biological molecules.", JOURNAL OF BIOLOGICAL PHYSICS, vol. 29, no. 2-3, 2003, pages 89 - 100, XP002450462, ISSN: 0092-0606 *
HARING BOLIVAR P ET AL: "Label-free probing of genes by time-domain terahertz sensing.", PHYSICS IN MEDICINE AND BIOLOGY 7 NOV 2002, vol. 47, no. 21, 7 November 2002 (2002-11-07), pages 3815 - 3821, XP002450463, ISSN: 0031-9155 *
NAGEL M ET AL: "INTEGRATED PLANAR TERAHERTZ RESONATORS FOR FEMTOMOLAR SENSITIVITY LABEL-FREE DETECTION DNA HYBRIDIZATION", APPLIED OPTICS, OSA, OPTICAL SOCIETY OF AMERICA, WASHINGTON, DC, US, vol. 41, no. 10, 1 April 2002 (2002-04-01), pages 2074 - 2078, XP001102355, ISSN: 0003-6935 *

Also Published As

Publication number Publication date
WO2007107956A2 (en) 2007-09-27
ITCN20060005A1 (en) 2007-09-23

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