WO2007107370A8 - Cytokine-based pyrogen test - Google Patents

Cytokine-based pyrogen test

Info

Publication number
WO2007107370A8
WO2007107370A8 PCT/EP2007/002562 EP2007002562W WO2007107370A8 WO 2007107370 A8 WO2007107370 A8 WO 2007107370A8 EP 2007002562 W EP2007002562 W EP 2007002562W WO 2007107370 A8 WO2007107370 A8 WO 2007107370A8
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
pyrogen
detecting
sample
endogenous
Prior art date
Application number
PCT/EP2007/002562
Other languages
French (fr)
Other versions
WO2007107370A1 (en
Inventor
Thomas Montag-Lessing
Ingo Spreitzer
Bettina Loeschner
Original Assignee
Paul Ehrlich Inst Bundesamt Fu
Thomas Montag-Lessing
Ingo Spreitzer
Bettina Loeschner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paul Ehrlich Inst Bundesamt Fu, Thomas Montag-Lessing, Ingo Spreitzer, Bettina Loeschner filed Critical Paul Ehrlich Inst Bundesamt Fu
Publication of WO2007107370A1 publication Critical patent/WO2007107370A1/en
Publication of WO2007107370A8 publication Critical patent/WO2007107370A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for detecting the presence of a pyrogen in a sample comprising the steps of subjecting said sample to a solution comprising monocytes, incubating the sample and the solution, detecting in one reaction vessel the presence of at least two endogenous pyrogens. The invention further pertains to the use of the inventive method for evaluating the safety or possible contamination of a product for use with humans or animals. Further the invention relates to a kit for performing the inventive method, wherein the kit comprises two or more anti-endogenous-pyrogen-antibodies for detecting a pyrogen or at least two nucleic acid probes or primers for detecting an mRNA of a gene encoding an endogenous pyrogen. The invention further relates in particular to an ELISA kit, more in particular to an ELISA kit comprising an anti-IL-6- antibody, anti-IL- IB-antibody, anti-TNF- α-antibody, anti-prostaglandine E2(PGE2)-antibody and/or anti-Neopterine-antibody.
PCT/EP2007/002562 2006-03-22 2007-03-22 Cytokine-based pyrogen test WO2007107370A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06005856.7 2006-03-22
EP06005856A EP1837658A1 (en) 2006-03-22 2006-03-22 Cytokine-based pyrogen test

Publications (2)

Publication Number Publication Date
WO2007107370A1 WO2007107370A1 (en) 2007-09-27
WO2007107370A8 true WO2007107370A8 (en) 2007-12-13

Family

ID=36636330

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/002562 WO2007107370A1 (en) 2006-03-22 2007-03-22 Cytokine-based pyrogen test

Country Status (2)

Country Link
EP (1) EP1837658A1 (en)
WO (1) WO2007107370A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009063307A2 (en) * 2007-11-14 2009-05-22 Bio-Technology General (Israel) Ltd. Inflammatory activity detection method using il-6
CN108535494A (en) * 2018-06-29 2018-09-14 湖南师范大学 A kind of IL-6ELISA detection kits and its application method
EP4286535A1 (en) * 2022-06-02 2023-12-06 Minerva Biolabs GmbH Method and detection kit for the detection of pyrogens in biological samples
WO2024052376A1 (en) * 2022-09-06 2024-03-14 Mat Research B.V. Improved monocyte activation test

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19516247A1 (en) * 1995-05-03 1996-11-07 Albrecht Prof Dr Wendel Pyrogen examination procedure
JP2003511696A (en) * 1999-10-13 2003-03-25 インサイト・ゲノミックス・インコーポレイテッド Multiple cytokine analysis

Also Published As

Publication number Publication date
WO2007107370A1 (en) 2007-09-27
EP1837658A1 (en) 2007-09-26

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