WO2007030373A3 - Method for in situ hybridization analysis - Google Patents

Method for in situ hybridization analysis Download PDF

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Publication number
WO2007030373A3
WO2007030373A3 PCT/US2006/034005 US2006034005W WO2007030373A3 WO 2007030373 A3 WO2007030373 A3 WO 2007030373A3 US 2006034005 W US2006034005 W US 2006034005W WO 2007030373 A3 WO2007030373 A3 WO 2007030373A3
Authority
WO
WIPO (PCT)
Prior art keywords
situ hybridization
hybridization analysis
temporal
ribonucleic acid
tissue sample
Prior art date
Application number
PCT/US2006/034005
Other languages
French (fr)
Other versions
WO2007030373A2 (en
Inventor
Thomas Curran
Dennis S Rice
Susan Magdaleno
Patricia Jensen
Original Assignee
St Jude Childrens Res Hospital
Thomas Curran
Dennis S Rice
Susan Magdaleno
Patricia Jensen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by St Jude Childrens Res Hospital, Thomas Curran, Dennis S Rice, Susan Magdaleno, Patricia Jensen filed Critical St Jude Childrens Res Hospital
Publication of WO2007030373A2 publication Critical patent/WO2007030373A2/en
Publication of WO2007030373A3 publication Critical patent/WO2007030373A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00673—Slice arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/0074—Biological products
    • B01J2219/00743—Cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples
    • G01N2001/368—Mounting multiple samples in one block, e.g. TMA [Tissue Microarrays]

Abstract

The present invention is a method for in situ hybridization analysis of the temporal and/or spatial expression of one or more ribonucleic acid target sequences in a tissue sample. The disclosed method can be adapted for high- throughput analysis of a plurality of target sequences and a plurality of tissue samples and the method is highly sensitive based upon the combination of a radiolabeled ribonucleic acid probe and silver emulsif ication of the tissue sample to detect the temporal and/or spatial expression of the target sequence.
PCT/US2006/034005 2005-09-07 2006-08-30 Method for in situ hybridization analysis WO2007030373A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71494905P 2005-09-07 2005-09-07
US60/714,949 2005-09-07

Publications (2)

Publication Number Publication Date
WO2007030373A2 WO2007030373A2 (en) 2007-03-15
WO2007030373A3 true WO2007030373A3 (en) 2009-04-23

Family

ID=37836349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/034005 WO2007030373A2 (en) 2005-09-07 2006-08-30 Method for in situ hybridization analysis

Country Status (1)

Country Link
WO (1) WO2007030373A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
PL2556171T3 (en) 2010-04-05 2016-04-29 Prognosys Biosciences Inc Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
DK3013984T3 (en) 2013-06-25 2023-06-06 Prognosys Biosciences Inc METHOD FOR DETERMINING SPATIAL PATTERNS IN BIOLOGICAL TARGETS IN A SAMPLE
ES2935860T3 (en) 2015-04-10 2023-03-13 Spatial Transcriptomics Ab Multiplex, spatially distinguished nucleic acid analysis of biological specimens
US11525759B2 (en) 2018-04-24 2022-12-13 Agilent Technologies, Inc. Deparaffinization of tissue utilizing electric field
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4222280A1 (en) * 2020-09-30 2023-08-09 Agilent Technologies, Inc. Electric field aided sample preparation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696271B2 (en) * 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696271B2 (en) * 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BOURAS. T. ET AL.: "Overexpression of the Steroid Receptor Coactivator AIB1 in Breast Cancer Correlates with the Absence of Estrogen and Progesterone Receptors and Positivity for p53 and HER2/neu", CANCER RESEARCH, vol. 61, February 2001 (2001-02-01), pages 903 - 907 *
OLSEN. L.: "CVD: the intestinal crypt/villus in situ hybridization database", BIOINFORMATICS, vol. 20, no. 8, February 2004 (2004-02-01), pages 1327 - 1328 *

Also Published As

Publication number Publication date
WO2007030373A2 (en) 2007-03-15

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