WO2007101866A3 - Mikroorganismus und verfahren zur herstellung von 2-methylzitronensäure - Google Patents

Mikroorganismus und verfahren zur herstellung von 2-methylzitronensäure Download PDF

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Publication number
WO2007101866A3
WO2007101866A3 PCT/EP2007/052136 EP2007052136W WO2007101866A3 WO 2007101866 A3 WO2007101866 A3 WO 2007101866A3 EP 2007052136 W EP2007052136 W EP 2007052136W WO 2007101866 A3 WO2007101866 A3 WO 2007101866A3
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WO
WIPO (PCT)
Prior art keywords
micro
producing
methylcitric acid
organism
methylcitric
Prior art date
Application number
PCT/EP2007/052136
Other languages
English (en)
French (fr)
Other versions
WO2007101866A2 (de
Inventor
Christian Ewering
Christian Oliver Braemer
Alexander Steinbuechel
Original Assignee
Univ Muenster Wilhelms
Christian Ewering
Christian Oliver Braemer
Alexander Steinbuechel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Muenster Wilhelms, Christian Ewering, Christian Oliver Braemer, Alexander Steinbuechel filed Critical Univ Muenster Wilhelms
Publication of WO2007101866A2 publication Critical patent/WO2007101866A2/de
Publication of WO2007101866A3 publication Critical patent/WO2007101866A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/48—Tricarboxylic acids, e.g. citric acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft rekombinante Mikroorganismen, die für die Herstellung von 2-Methylzitronensäure geeignet sind, sowie Verfahren zur Herstellung von 2-Methylzitronensäure unter Verwendung dieser Mikroorganismen. Gegenstand der Erfindung sind insbesondere Mikroorganismen, die für die biotechnologische Herstellung von 2-Methylzitronensäure in Fermentationsverfahren geeignet sind, sowie Fermentationsverfahren zur Herstellung von 2-Methylzitronensäure die unter Verwendung der erfindungsgemäßen Mikroorganismus durchgeführt werden.
PCT/EP2007/052136 2006-03-08 2007-03-07 Mikroorganismus und verfahren zur herstellung von 2-methylzitronensäure WO2007101866A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006010786A DE102006010786A1 (de) 2006-03-08 2006-03-08 Mikroorganismus und Verfahren zur Herstellung von 2-Methylzitronensäure
DE102006010786.1 2006-03-08

Publications (2)

Publication Number Publication Date
WO2007101866A2 WO2007101866A2 (de) 2007-09-13
WO2007101866A3 true WO2007101866A3 (de) 2007-11-22

Family

ID=38335941

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/052136 WO2007101866A2 (de) 2006-03-08 2007-03-07 Mikroorganismus und verfahren zur herstellung von 2-methylzitronensäure

Country Status (2)

Country Link
DE (1) DE102006010786A1 (de)
WO (1) WO2007101866A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008041299A1 (de) 2008-08-18 2010-02-25 Evonik Degussa Gmbh Neuartiges, universell einsetzbares addiction-System

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
BRÄMER C O ET AL: "Identification of the 2-methylcitrate pathway involved in the catabolism of propionate in the polyhydroxyalkanoate-producing strain Burkholderia sacchari IPT101(T) and analysis of a mutant accumulating a copolyester with higher 3-hydroxyvalerate content.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY JAN 2002, vol. 68, no. 1, January 2002 (2002-01-01), pages 271 - 279, XP002449883, ISSN: 0099-2240 *
BRÄMER C O ET AL: "The methylcitric acid pathway in Ralstonia eutropha: new genes identified involved in propionate metabolism.", MICROBIOLOGY (READING, ENGLAND) AUG 2001, vol. 147, no. Pt 8, August 2001 (2001-08-01), pages 2203 - 2214, XP002449882, ISSN: 1350-0872 *
CHRISTIAN EWERING ET AL: "Occurrence and expression of tricarboxylate synthases in Ralstonia eutropha", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER-VERLAG, BE, vol. 71, no. 1, 23 August 2005 (2005-08-23), pages 80 - 89, XP019422031, ISSN: 1432-0614 *
CLAES WILFRIED A ET AL: "Identification of two prpDBC gene clusters in Corynebacterium glutamicum and their involvement in propionate degradation via the 2-methylcitrate cycle", JOURNAL OF BACTERIOLOGY, vol. 184, no. 10, May 2002 (2002-05-01), pages 2728 - 2739, XP002449885, ISSN: 0021-9193 *
DATABASE EMBL [online] 17 September 2001 (2001-09-17), "Ralstonia eutropha strain HF39 methylcitrate pathway gene cluster, complete sequence.", XP002449890, retrieved from EBI accession no. EMBL:AF325554 Database accession no. AF325554 *
DATABASE UniProt [online] 1 June 2003 (2003-06-01), "Methylcitrate synthase, putative.", XP002449891, retrieved from EBI accession no. UNIPROT:Q88KF5 Database accession no. Q88KF5 *
EWERING ET AL: "Metabolic engineering of strains of Ralstonia eutropha and Pseudomonas putida for biotechnological production of 2-methylcitric acid", METABOLIC ENGINEERING, ACADEMIC PRESS,, US, vol. 8, no. 6, 9 November 2006 (2006-11-09), pages 587 - 602, XP005844549, ISSN: 1096-7176 *
HORSWILL ALEXANDER R ET AL: "In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-Methylcitrate dehydratase (prpD) and aconitase enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate", BIOCHEMISTRY, vol. 40, no. 15, 17 April 2001 (2001-04-17), pages 4703 - 4713, XP002449886, ISSN: 0006-2960 *
HORSWILL ALEXANDER R ET AL: "Propionate catabolism in Salmonella typhimurium LT2: Two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon", JOURNAL OF BACTERIOLOGY, vol. 179, no. 3, 1997, pages 928 - 940, XP002449884, ISSN: 0021-9193 *
NELSON K E ET AL: "Complete genome sequence and comparative analysis of the metabolically versatile pseudomonas putida KT2440", ENVIRONMENTAL MICROBIOLOGY, BLACKWELL SCIENCE, OXFORD, GB, vol. 4, no. 12, December 2002 (2002-12-01), pages 799 - 808, XP002968819, ISSN: 1462-2912 *
POHLMANN ANNE ET AL: "Genome sequence of the bioplastic-producing "Knallgas" bacterium Ralstonia eutropha H16", NATURE BIOTECHNOLOGY, vol. 24, no. 10, October 2006 (2006-10-01), pages 1257 - 1262, XP002449887, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2007101866A2 (de) 2007-09-13
DE102006010786A1 (de) 2007-09-13

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