WO2007096830A3 - External cell control quantitative assay - Google Patents

External cell control quantitative assay Download PDF

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Publication number
WO2007096830A3
WO2007096830A3 PCT/IB2007/050571 IB2007050571W WO2007096830A3 WO 2007096830 A3 WO2007096830 A3 WO 2007096830A3 IB 2007050571 W IB2007050571 W IB 2007050571W WO 2007096830 A3 WO2007096830 A3 WO 2007096830A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
cells
interest
present
target nucleic
Prior art date
Application number
PCT/IB2007/050571
Other languages
French (fr)
Other versions
WO2007096830A2 (en
Inventor
Tamas Aranyi
Andras Bors
Anna Brozik
Tuende Kovacs
Maria Magocsi
Gabriella Mehes
Polett Ribiczey
Attila Tordai
Zsuzsanna Ujfaludi
Andras Varadi
Original Assignee
Inst Of Enzymology Of The Biol
Tamas Aranyi
Andras Bors
Anna Brozik
Tuende Kovacs
Maria Magocsi
Gabriella Mehes
Polett Ribiczey
Attila Tordai
Zsuzsanna Ujfaludi
Andras Varadi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Of Enzymology Of The Biol, Tamas Aranyi, Andras Bors, Anna Brozik, Tuende Kovacs, Maria Magocsi, Gabriella Mehes, Polett Ribiczey, Attila Tordai, Zsuzsanna Ujfaludi, Andras Varadi filed Critical Inst Of Enzymology Of The Biol
Publication of WO2007096830A2 publication Critical patent/WO2007096830A2/en
Publication of WO2007096830A3 publication Critical patent/WO2007096830A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method of determining the quantity of a target nucleic acid or protein in cells of interest comprised in a biological sample characterized by a) mixing said cells of interest containing the target nucleic acid or protein, in a predetermined ratio, with control cells that contain a reference nucleic acid not present in said cells of interest, wherein the control cells do not contain the target nucleic acid, b) subjecting the mixture of said cells of interest and said control cells obtained in step a) to a cell lysis procedure according to an established protocol, and c) determining the quantity of the target nucleic acid present in said cell lysate relative to the amount of said reference nucleic acid by performing an established protocol designed to determine the amount of cellular nucleic acids. The present invention also relates to kits performing said methods according to the invention.
PCT/IB2007/050571 2006-02-23 2007-02-22 External cell control quantitative assay WO2007096830A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU0600159A HU0600159D0 (en) 2006-02-23 2006-02-23 Kvantitative pcr
HUP0600159 2006-02-23

Publications (2)

Publication Number Publication Date
WO2007096830A2 WO2007096830A2 (en) 2007-08-30
WO2007096830A3 true WO2007096830A3 (en) 2007-11-22

Family

ID=89986605

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2007/050571 WO2007096830A2 (en) 2006-02-23 2007-02-22 External cell control quantitative assay

Country Status (2)

Country Link
HU (1) HU0600159D0 (en)
WO (1) WO2007096830A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150252413A1 (en) * 2011-11-07 2015-09-10 Bioquanta Method for normalization of quantitative pcr and microarrays

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023023A1 (en) * 1993-04-06 1994-10-13 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
DE19718705A1 (en) * 1997-05-02 1998-11-05 Christoph Dr Fiehn Method for the quantitative determination of the number of transgene copies in genetically modified cells
EP1277842A2 (en) * 2001-07-17 2003-01-22 Fuji Photo Film Co., Ltd. Method for quantifying nucleic acid by cell counting
EP1435393A1 (en) * 2002-12-12 2004-07-07 Fuji Photo Film Co., Ltd. Method for analyzing gene expression levels by quantification of pyrophosphoric acid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023023A1 (en) * 1993-04-06 1994-10-13 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
DE19718705A1 (en) * 1997-05-02 1998-11-05 Christoph Dr Fiehn Method for the quantitative determination of the number of transgene copies in genetically modified cells
EP1277842A2 (en) * 2001-07-17 2003-01-22 Fuji Photo Film Co., Ltd. Method for quantifying nucleic acid by cell counting
EP1435393A1 (en) * 2002-12-12 2004-07-07 Fuji Photo Film Co., Ltd. Method for analyzing gene expression levels by quantification of pyrophosphoric acid

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FERRE F: "QUANTITATIVE OR SEMI-QUANTITATIVE PCR: REALITY VERSUS MYTH", PCR METHODS AND APPLICATIONS, COLD SPRING HARBOR, NY, US, vol. 2, no. 1, August 1992 (1992-08-01), pages 1 - 9, XP009018224, ISSN: 1054-9803 *

Also Published As

Publication number Publication date
HU0600159D0 (en) 2006-04-28
WO2007096830A2 (en) 2007-08-30

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