WO2005090599A3 - Methods and adaptors for analyzing specific nucleic acid populations - Google Patents
Methods and adaptors for analyzing specific nucleic acid populations Download PDFInfo
- Publication number
- WO2005090599A3 WO2005090599A3 PCT/GB2005/000989 GB2005000989W WO2005090599A3 WO 2005090599 A3 WO2005090599 A3 WO 2005090599A3 GB 2005000989 W GB2005000989 W GB 2005000989W WO 2005090599 A3 WO2005090599 A3 WO 2005090599A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- adaptor
- tester
- driver
- acid complexes
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
Abstract
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0405878A GB2412170A (en) | 2004-03-16 | 2004-03-16 | A method for enriching for target nucleic acids in a sample |
GB0405878.0 | 2004-03-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2005090599A2 WO2005090599A2 (en) | 2005-09-29 |
WO2005090599A3 true WO2005090599A3 (en) | 2006-06-01 |
Family
ID=32117779
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2005/000989 WO2005090599A2 (en) | 2004-03-16 | 2005-03-15 | Methods and adaptors for analyzing specific nucleic acid populations |
Country Status (2)
Country | Link |
---|---|
GB (1) | GB2412170A (en) |
WO (1) | WO2005090599A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9404150B2 (en) | 2007-08-29 | 2016-08-02 | Sequenom, Inc. | Methods and compositions for universal size-specific PCR |
US9512478B2 (en) | 2007-02-02 | 2016-12-06 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple polynucleotide templates |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0514910D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Method for sequencing a polynucleotide template |
GB0514935D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Methods for sequencing a polynucleotide template |
GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
GB0524069D0 (en) | 2005-11-25 | 2006-01-04 | Solexa Ltd | Preparation of templates for solid phase amplification |
DK1987159T4 (en) | 2006-02-08 | 2020-11-16 | Illumina Cambridge Ltd | PROCEDURE FOR SEQUENCE OF A POLYNUCLEOTID TEMPLATE |
WO2008015396A2 (en) * | 2006-07-31 | 2008-02-07 | Solexa Limited | Method of library preparation avoiding the formation of adaptor dimers |
US8932994B2 (en) | 2006-08-24 | 2015-01-13 | Illumina, Inc. | Method for retaining even coverage of short insert libraries |
US7754429B2 (en) | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
US7902345B2 (en) | 2006-12-05 | 2011-03-08 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
WO2008096146A1 (en) | 2007-02-07 | 2008-08-14 | Solexa Limited | Preparation of templates for methylation analysis |
WO2011025477A1 (en) | 2009-08-25 | 2011-03-03 | Illumina, Inc. | Methods for selecting and amplifying polynucleotides |
EP2408936A4 (en) * | 2009-03-18 | 2013-01-30 | Sequenom Inc | Use of thermostable endonucleases for generating reporter molecules |
EP4055188A4 (en) * | 2019-11-05 | 2023-11-22 | Ultima Genomics, Inc. | Methods for preparing a library of polynucleotide molecules |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6060245A (en) * | 1996-12-13 | 2000-05-09 | Stratagene | Methods and adaptors for generating specific nucleic acid populations |
US6090548A (en) * | 1996-05-14 | 2000-07-18 | Roche Diagnostics Gmbh | Method for identifying and/or quantifying expression of nucleic acid molecules in a sample |
US6316192B1 (en) * | 1999-03-11 | 2001-11-13 | Jianhua Luo | Method for enrichment of unique DNA fragments through cyclical removal of PCR adapter attached to DNA fragments whose sequences are shared between two DNA pools |
US6372434B1 (en) * | 1998-09-18 | 2002-04-16 | Molecular Staging, Inc. | Methods for reducing the complexity of DNA sequences |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5436142A (en) * | 1992-11-12 | 1995-07-25 | Cold Spring Harbor Laboratory | Methods for producing probes capable of distingushing variant genomic sequences |
US5804382A (en) * | 1996-05-10 | 1998-09-08 | Beth Israel Deaconess Medical Center, Inc. | Methods for identifying differentially expressed genes and differences between genomic nucleic acid sequences |
US5935788A (en) * | 1996-06-25 | 1999-08-10 | Lifespan Biosciences, Inc. | Subtractive hybridization techniques for identifying differentially expressed and commonly expressed nucleic acid |
US6455255B1 (en) * | 2000-08-02 | 2002-09-24 | Abbott Laboratories | Method of performing subtractive hybridization using RDA |
WO2002103007A1 (en) * | 2001-06-19 | 2002-12-27 | Eisai C0. Ltd. | Method of uniformizing dna fragment contents and subtraction method |
-
2004
- 2004-03-16 GB GB0405878A patent/GB2412170A/en not_active Withdrawn
-
2005
- 2005-03-15 WO PCT/GB2005/000989 patent/WO2005090599A2/en active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6090548A (en) * | 1996-05-14 | 2000-07-18 | Roche Diagnostics Gmbh | Method for identifying and/or quantifying expression of nucleic acid molecules in a sample |
US6060245A (en) * | 1996-12-13 | 2000-05-09 | Stratagene | Methods and adaptors for generating specific nucleic acid populations |
US6372434B1 (en) * | 1998-09-18 | 2002-04-16 | Molecular Staging, Inc. | Methods for reducing the complexity of DNA sequences |
US6316192B1 (en) * | 1999-03-11 | 2001-11-13 | Jianhua Luo | Method for enrichment of unique DNA fragments through cyclical removal of PCR adapter attached to DNA fragments whose sequences are shared between two DNA pools |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9512478B2 (en) | 2007-02-02 | 2016-12-06 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple polynucleotide templates |
US9404150B2 (en) | 2007-08-29 | 2016-08-02 | Sequenom, Inc. | Methods and compositions for universal size-specific PCR |
Also Published As
Publication number | Publication date |
---|---|
GB0405878D0 (en) | 2004-04-21 |
GB2412170A (en) | 2005-09-21 |
WO2005090599A2 (en) | 2005-09-29 |
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