WO2007092185A3 - Assays and enhancers of the human delta enac sodium channel - Google Patents

Assays and enhancers of the human delta enac sodium channel Download PDF

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Publication number
WO2007092185A3
WO2007092185A3 PCT/US2007/002293 US2007002293W WO2007092185A3 WO 2007092185 A3 WO2007092185 A3 WO 2007092185A3 US 2007002293 W US2007002293 W US 2007002293W WO 2007092185 A3 WO2007092185 A3 WO 2007092185A3
Authority
WO
WIPO (PCT)
Prior art keywords
assays
enhancers
sodium channel
delta
human delta
Prior art date
Application number
PCT/US2007/002293
Other languages
French (fr)
Other versions
WO2007092185A2 (en
Inventor
Bryan Moyer
Min Lu
Fernando Echeverri
Original Assignee
Senomyx Inc
Bryan Moyer
Min Lu
Fernando Echeverri
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Senomyx Inc, Bryan Moyer, Min Lu, Fernando Echeverri filed Critical Senomyx Inc
Priority to US12/278,064 priority Critical patent/US10078087B2/en
Publication of WO2007092185A2 publication Critical patent/WO2007092185A2/en
Publication of WO2007092185A3 publication Critical patent/WO2007092185A3/en
Priority to US16/130,017 priority patent/US20190154702A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G01N33/14—Beverages

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

This invention relates to electrophysiological assays that measure sodium conductance activity of a delta human epithelial sodium channel (ENaC) in the presence and absence of delta hENaC enhancers. Also, the invention generally relates to assays for identifying compounds that enhance the activity of delta hENaC, especially in an oocyte expression system. These compounds have potential application in modulating (enhancing) salty taste perception.
PCT/US2007/002293 2001-05-01 2007-01-26 Assays and enhancers of the human delta enac sodium channel WO2007092185A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/278,064 US10078087B2 (en) 2001-05-01 2007-01-26 Assays and enhancers of the human delta ENaC sodium channel
US16/130,017 US20190154702A1 (en) 2001-05-01 2018-09-13 Assays and enhancers of the human delta enac sodium channel

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US76435306P 2006-02-02 2006-02-02
US60/764,353 2006-02-02

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2004/021853 Continuation-In-Part WO2005014848A2 (en) 2001-05-01 2004-07-09 IMPROVED ELECTROPHYSIOLOGICAL ASSAYS USING OOCYTES THAT EXPRESS HUMAN ENaC AND THE USE OF PHENAMIL TO IMPROVE THE EFFECT OF ENaC ENHANCERS IN ASSAYS USING MEMBRANE POTENTIAL REPORTING DYES
US10/563,758 Continuation-In-Part US9459259B2 (en) 2001-05-01 2004-07-09 Electrophysiological assays using oocytes that express human enac and the use of phenamil to improve the effect of enac enhancers in assays using membrane potential reporting dyes

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/278,064 A-371-Of-International US10078087B2 (en) 2001-05-01 2007-01-26 Assays and enhancers of the human delta ENaC sodium channel
US16/130,017 Division US20190154702A1 (en) 2001-05-01 2018-09-13 Assays and enhancers of the human delta enac sodium channel

Publications (2)

Publication Number Publication Date
WO2007092185A2 WO2007092185A2 (en) 2007-08-16
WO2007092185A3 true WO2007092185A3 (en) 2008-02-28

Family

ID=38345626

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/002293 WO2007092185A2 (en) 2001-05-01 2007-01-26 Assays and enhancers of the human delta enac sodium channel

Country Status (1)

Country Link
WO (1) WO2007092185A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008051447A2 (en) 2006-10-19 2008-05-02 Monell Chemical Senses Center Human salty taste receptor and methods of modulating salty taste perception
EP2245057B1 (en) 2008-01-25 2019-09-04 Chromocell Corporation Novel cell lines expressing enac and methods using them

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002087306A2 (en) * 2001-05-01 2002-11-07 Senomyx, Inc. High throughput cell-based assay for monitoring sodium channel activity and discovery of salty taste modulating compounds
US20050059094A1 (en) * 2001-05-01 2005-03-17 Guy Servant Electrophysiological assay using oocytes that express human ENaC and the use phenamil to improve the effect of ENaC enhancers in assays using membrane potential reporting dyes
US20060223117A1 (en) * 2005-02-07 2006-10-05 Senomyx, Inc. Novel splice variants of human epithelial sodium channel genes expressed in human taste tissue and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002087306A2 (en) * 2001-05-01 2002-11-07 Senomyx, Inc. High throughput cell-based assay for monitoring sodium channel activity and discovery of salty taste modulating compounds
US20050059094A1 (en) * 2001-05-01 2005-03-17 Guy Servant Electrophysiological assay using oocytes that express human ENaC and the use phenamil to improve the effect of ENaC enhancers in assays using membrane potential reporting dyes
US20060223117A1 (en) * 2005-02-07 2006-10-05 Senomyx, Inc. Novel splice variants of human epithelial sodium channel genes expressed in human taste tissue and uses thereof

Also Published As

Publication number Publication date
WO2007092185A2 (en) 2007-08-16

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