WO2007087813A2 - Egyptian strain of entomopathogenic fungus beauveria bassiana - Google Patents

Egyptian strain of entomopathogenic fungus beauveria bassiana Download PDF

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Publication number
WO2007087813A2
WO2007087813A2 PCT/EG2006/000006 EG2006000006W WO2007087813A2 WO 2007087813 A2 WO2007087813 A2 WO 2007087813A2 EG 2006000006 W EG2006000006 W EG 2006000006W WO 2007087813 A2 WO2007087813 A2 WO 2007087813A2
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Prior art keywords
strain
weevil
red palm
palm weevil
fungal strain
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PCT/EG2006/000006
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French (fr)
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WO2007087813A3 (en
Inventor
Gamal El Din Hassan Sewify
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Arab Organization For Agricultural And Development
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Publication date
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Priority to PCT/EG2006/000006 priority Critical patent/WO2007087813A2/en
Publication of WO2007087813A2 publication Critical patent/WO2007087813A2/en
Publication of WO2007087813A3 publication Critical patent/WO2007087813A3/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi

Definitions

  • red palm weevil The method currently employed to control red palm weevil are largely quantities of synthetic chemical insecticides , this lead to the pollution of soil and water course around areas with palm weevil infestation .
  • other techniques of management such as sanitation , baits and traps of palm weevils have been used .
  • Direct injection of insecticides into the trunk and intensive regular spraying of insecticides were employed to control this insed .
  • the development of IPM for red palm weevil through the integration of biological control will reduce the using of insecticides .
  • the prospects for the development of a biological control component for an integrated management strategy are good , given the evidence for the importance of natural enemies in controlling palm weevil population in natural environment .
  • the fungus B . bassiana has been successfully used as biocontrol agent for the management of number of coleopteran insects . All red palm weevil stages showed highly susceptibility to fungal infection under laboratory tests . Disclosure of the invention :
  • the entomopathogenic fungus Beauveria bassiana strain ( AGl ) was isolated from insect cadaver of adult stage of red palm weevil Rhynchophorus ferrugineus in Ismailia governorate during 2001 . Fungal strain proved highly pathogenicty to all stages of red palm weevil . The early instar larvae appeared high susceptibility to fungal infection follwed by adult stage .
  • the bioassay procedures showed that the LC 50 values (the lethal mortality for 50 % of treaded insects ) of fungal spores concentration for 3 rd larval instar , 9 th larval instar and adult stage were 4.3 x 10 6 , 1.7 x 10 7 and 8.I x IO 6 spores / ml respectvily .
  • the LC 5 0 lethal time mortality for 50 % of treated insects ) was 2.7 days for adult stage .
  • the strains AGl proved highly pathogencity to red palm weevil than other strains .

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

AGI strain of entomopathogenic fungus B . bassiana was isolated from cadaver of adult stage of red palm weevil Rhychophorus ferrugineus . The red palm weevil is destructive insect and it causes serious damage to date palms in Egypt . Recently , the weevil has spread widely and now is the most economically important pest of the date palm in Egypt . The biocontrol means by using entomopathogenic fungi is more needed to control such this insect .All stages of red palm weevil appeared highly susceptibility to AGI fungal strain . The fungal strain appeared highly pathogenicty , viability and sporulation compared with other 44 tested other strains of fungs B . bassiana . Genetic description of fungal strain was conducted . Field application of this fungal strain in large scale proved highly control to red palm weevil .

Description

Egyptian Strain Of Entomopathogenic Fungus Beauveria bassiana
Technical Field : Biological control of Agricultural insect pests by using the entomopathogenic fungi .
Background art : The date palm , is the most important fruit crop in Egypt as well as the Middle East. In the mid 1990 s1 a dreaded pest of palms , the red palm weevil Rhynchσphorus ferrugineus, was reported to cause serious damage to date palms in Egypt . Since , this time , the weevil has spread widely and now is the most economically important pest of the date palm in Egypt . Eggs of R . ferrugineus are laid into the trunk of the palm and the larval stage feed on within the trunk , which leads to the formation of tunnels inside the palm . Frequent and heavy attacks weaken and eventually kill the tree . The method currently employed to control red palm weevil are largely quantities of synthetic chemical insecticides , this lead to the pollution of soil and water course around areas with palm weevil infestation . However , other techniques of management such as sanitation , baits and traps of palm weevils have been used . Direct injection of insecticides into the trunk and intensive regular spraying of insecticides were employed to control this insed . The development of IPM for red palm weevil through the integration of biological control will reduce the using of insecticides . The prospects for the development of a biological control component for an integrated management strategy are good , given the evidence for the importance of natural enemies in controlling palm weevil population in natural environment . The fungus B . bassiana has been successfully used as biocontrol agent for the management of number of coleopteran insects . All red palm weevil stages showed highly susceptibility to fungal infection under laboratory tests . Disclosure of the invention :
The entomopathogenic fungus Beauveria bassiana strain ( AGl ) was isolated from insect cadaver of adult stage of red palm weevil Rhynchophorus ferrugineus in Ismailia Governorate during 2001 . Fungal strain proved highly pathogenicty to all stages of red palm weevil . The early instar larvae appeared high susceptibility to fungal infection follwed by adult stage . The bioassay procedures showed that the LC50 values ( the lethal mortality for 50 % of treaded insects ) of fungal spores concentration for 3rd larval instar , 9th larval instar and adult stage were 4.3 x 106 , 1.7 x 107 and 8.I x IO6 spores / ml respectvily . However , the LC50 ( lethal time mortality for 50 % of treated insects ) was 2.7 days for adult stage . Among 44 strains of fungus B . bassiana isolated from different regions in Egypt , the strains AGl proved highly pathogencity to red palm weevil than other strains . This strain appeared higher viability ( 99 % spore germination ) and highly sporulation ( 4.6 x 107 spores / ml ) compored with other tested fungal stains . Field application with fungus B . bassiana against red palm weevil were conducted through 2002 - 2005 . In the field using the fungal applied injection was an effective method for controlling red palm weevil in naturally infested palm trees . The success of the treatment was up 90 % . The periodical spray application of fungal spores on palm trees reduced red palm weevil population . Reduction of palm trees infestation following field application was noticed . Release of contaminated males of red palm weevil with fungal spores was carried out weekly in infested regions . This method proved significant reduction in the palm weevil population . These results suggests that fungus B . bassiana could be an effective and economically feasible control for the red palm weevil .
Genetic characterization :
Finger print of the fungus Beauveria basiana strain ( AGl ) was carried out as shown in fig ( 1 ) . The description of the fungal as following :
Figure imgf000004_0001

Claims

Claims 1- Egyptian strain of entomopathogenic fungus Beauveria basiana ( AGl ) was isolated from cadaver of red palm weevil . 2- Genetic characterization according to claim 1 , wherein said was carried out to describe the fungal strain . 3- The pathogenicity according to claim 1 , wherein said was highly against red palm weevil stages . 4- The spores viability according to claim 1 , wherein said was high reached to 99 % . 5- Fungal sporulation according to claim 1 , wherein said was high in insect cadavers . 6- Mass production of AGl according to claim 1 , wherein said can be produced in large scales . 7- The AGl strain of fungus B. hassiana used mainly to control the red palm weevil. 8- The AGl strain of fungus B . bassian according to claim 1 , wherein said can be used to control other pests .
PCT/EG2006/000006 2006-02-01 2006-02-01 Egyptian strain of entomopathogenic fungus beauveria bassiana WO2007087813A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/EG2006/000006 WO2007087813A2 (en) 2006-02-01 2006-02-01 Egyptian strain of entomopathogenic fungus beauveria bassiana

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EG2006/000006 WO2007087813A2 (en) 2006-02-01 2006-02-01 Egyptian strain of entomopathogenic fungus beauveria bassiana

Publications (2)

Publication Number Publication Date
WO2007087813A2 true WO2007087813A2 (en) 2007-08-09
WO2007087813A3 WO2007087813A3 (en) 2010-10-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EG2006/000006 WO2007087813A2 (en) 2006-02-01 2006-02-01 Egyptian strain of entomopathogenic fungus beauveria bassiana

Country Status (1)

Country Link
WO (1) WO2007087813A2 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2170084A1 (en) * 2007-06-05 2010-04-07 Universidad De Alicante Phytosanitary composition comprising an entomopathogenic fungus belonging to the species b. bassiana and triturates or fragments of dates, method of preparation and use of same
CN110106093A (en) * 2019-05-17 2019-08-09 江西省科学院微生物研究所 A kind of Strain of Beauveria bassiana and its application
CZ308112B6 (en) * 2019-03-12 2020-01-08 Jihočeská Univerzita V Českých Budějovicích Entomopathogenic fungi strain Beauveria bassiana, method of its use and a preparation containing spore strain
KR20210142498A (en) * 2020-05-18 2021-11-25 전북대학교산학협력단 Composition for controlling Rhynchophorus ferrugineus comprising Beauveria bassiana JEF-507 as an active ingredient
CN114806898A (en) * 2022-06-13 2022-07-29 云南农业大学 Beauveria rhynchophylla BbKMND202111 strain and application thereof
CN115074258A (en) * 2022-07-18 2022-09-20 广东海洋大学 Beauveria bassiana Bals-1722 and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002028189A2 (en) * 2000-10-04 2002-04-11 Paul Stamets Mycoattractants and mycopesticides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002028189A2 (en) * 2000-10-04 2002-04-11 Paul Stamets Mycoattractants and mycopesticides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DEADMAN M.L. ET AL.: 'Preliminary investigations into the biological control of red palm weevil using beauveria bassiana.' PROCEEDINGS OF THE SECOND INTERNATIONAL CONFERENCE ON DATE PALMS (AI-AIN, UAE), [Online] 25 March 2001 - 27 March 2001, pages 225 - 232 Retrieved from the Internet: <URL:http:www.pubhort.org/datepalm> [retrieved on 2006-10-03] *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2170084A1 (en) * 2007-06-05 2010-04-07 Universidad De Alicante Phytosanitary composition comprising an entomopathogenic fungus belonging to the species b. bassiana and triturates or fragments of dates, method of preparation and use of same
EP2170084A4 (en) * 2007-06-05 2013-06-19 Univ Alicante Phytosanitary composition comprising an entomopathogenic fungus belonging to the species b. bassiana and triturates or fragments of dates, method of preparation and use of same
CZ308112B6 (en) * 2019-03-12 2020-01-08 Jihočeská Univerzita V Českých Budějovicích Entomopathogenic fungi strain Beauveria bassiana, method of its use and a preparation containing spore strain
CN110106093A (en) * 2019-05-17 2019-08-09 江西省科学院微生物研究所 A kind of Strain of Beauveria bassiana and its application
CN110106093B (en) * 2019-05-17 2021-11-26 江西省科学院微生物研究所 Beauveria bassiana strain and application thereof
KR20210142498A (en) * 2020-05-18 2021-11-25 전북대학교산학협력단 Composition for controlling Rhynchophorus ferrugineus comprising Beauveria bassiana JEF-507 as an active ingredient
KR102342488B1 (en) 2020-05-18 2021-12-23 전북대학교산학협력단 Composition for controlling Rhynchophorus ferrugineus comprising Beauveria bassiana JEF-507 as an active ingredient
CN114806898A (en) * 2022-06-13 2022-07-29 云南农业大学 Beauveria rhynchophylla BbKMND202111 strain and application thereof
CN115074258A (en) * 2022-07-18 2022-09-20 广东海洋大学 Beauveria bassiana Bals-1722 and application thereof
CN115074258B (en) * 2022-07-18 2024-03-15 广东海洋大学 Beauveria bassiana Bals-1722 and application thereof

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Publication number Publication date
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