WO2007086397A1

WO2007086397A1 - Fat composition for ameliorating lipid metabolism

Info

Publication number: WO2007086397A1
Application number: PCT/JP2007/051043
Authority: WO
Inventors: Nobuo Sagi; Nobuhiko Tachibana; Haruyasu Kida; Kiyoharu Takamatsu
Original assignee: Fuji Oil Company, Limited
Priority date: 2006-01-30
Filing date: 2007-01-24
Publication date: 2007-08-02
Also published as: JPWO2007086397A1

Abstract

A fat composition for lipid metabolism amelioration which has improved safety and has the functions of inhibiting weight increase, inhibiting body fat accumulation, and reducing natural lipids in the blood and liver lipids. The fat composition for lipid metabolism amelioration has a composition of constituent fatty acids in which S/U is 0.6-2.5 and C12/U is 0.4-1.5 and a composition of constituent triglycerides in which the triglycerides in which the total number of carbon atoms in the acyl groups is 32-36 account for less than 30% of all the triglycerides and the triglycerides in which the total number of carbon atoms in the acyl groups is 42-48 account for 35% or more of all the triglycerides.

Description

Specification

Oil composition for improving lipid metabolism

Technical field

[0001] The present invention relates to an oil and fat composition for improving lipid metabolism.

Background art

[0002] In recent years, with the Westernization of dietary habits, lipid intake has increased in Japanese diets, and the share of lipids in intake energy has tripled 50 years ago. This is closely related to obesity and hyperlipidemia, diabetes and hypertension, which are lifestyle-related diseases derived from increased blood triglyceride (triglyceride) and blood cholesterol levels. And is thought to induce cerebrovascular disease. Since these are closely related to arteriosclerosis, keeping these values low is particularly important for preventing lifestyle-related diseases, and for obesity prevention, the importance of quality not only by the amount of food is important. It has been reported. However, fats and oils are indispensable for the taste of food, and strength and fats also have a cooking function as a heat medium, so it is difficult to simply reduce the intake.

[0003] For this reason, development of various functional foods / materials aimed at improving lipid metabolism has become active. The powers proposed and commercialized as described in Patent Documents 1 to 4 all have the disadvantage that it is difficult to say that they are natural fats and oils. In particular, Patent Documents 1 to 3 disclose an oil and fat composition containing diglyceride as an active ingredient and having little body fat accumulation, but the safety of using an oil and fat composition rich in diglyceride as an edible food is completely It is not proved to be.

[0004] Patent Document 1: Japanese Patent Laid-Open No. 4 300826

Patent Document 2: JP-A-8-60180

Patent Document 3: Japanese Patent Laid-Open No. 10-176181

Patent Document 4: Japanese Unexamined Patent Publication No. 2000-309794

Disclosure of the invention

Problems to be solved by the invention

[0005] An object of the present invention is to suppress body weight gain and body fat accumulation with improved safety, and An object of the present invention is to provide an oil and fat composition for improving lipid metabolism having a function of reducing neutral lipids in blood and liver lipids.

Means for solving the problem

[0006] As a result of diligent research to solve the above problems, the present inventors have unexpectedly found that the ratio of saturated fatty acid mainly composed of lauric acid to long-chain monounsaturated fatty acid mainly composed of oleic acid is specified. It was found that an oil thread and a composition having a specific triglyceride thread within the range has a function of suppressing weight gain and body fat accumulation and a function of reducing neutral lipids in blood and liver lipids. The invention has been completed.

That is, the present invention relates to

(1) The composition of fatty acids is SZU 0.6 to 2.5, C12ZU is 0.4 to 1.5, and the composition of triglycerides is a total of 32 to 36 carbon atoms in the asil group. An oil / fat composition for improving lipid metabolism, comprising a triglyceride containing less than 30% of a triglyceride and a triglyceride having a total power of 2 to 48 carbon atoms of an acyl group in an amount of 35% or more. Where S: total saturated fatty acid content in weight percent as methyl ester, U: total unsaturated fatty acid content in weight percent as methyl ester.

(2) The fat composition for improving lipid metabolism according to (1), obtained by fractionating palm kernel oil.

(3) The fat and oil composition for improving lipid metabolism according to (1) and (2), which is obtained by fractionating palm kernel oil a plurality of times.

(4) The oil / fat composition for improving lipid metabolism according to (1) or (2), obtained by dry fractionating palm kernel oil.

It is.

The invention's effect

[0008] As described above, in the composition of the constituent fatty acids, SZU is 0.6 to 2.5, C12ZU is 0.5.

The triglyceride composition is 4 to 1.5 and the total triglyceride composition has 32 to 36 carbon atoms in the acyl group is less than 30% of the total, and the total carbon power of the acyl group is 2 to 48. An oil / fat composition for improving lipid metabolism containing at least 35% of a triglyceride is used to suppress weight gain and body fat accumulation, as well as neutral fat in blood and liver lipids. Quality can be reduced.

BEST MODE FOR CARRYING OUT THE INVENTION

[0009] The fat and oil composition for improving lipid metabolism of the present invention can be widely used for foods usually containing fats and oils. For example, liquid coffee cream typified by porcelain cream 'margarine' mayonnaise 'dressing' used as emulsified foods such as dairy products, ice cream confections, chocolate confectionery, breads, spray fats and oils It can be added to snacks and confectionery, processed meat products such as ham 'sausage', seafood processed foods such as power maboko 'chikuwa', etc. without sacrificing flavor and texture.

[0010] The fat and oil composition for improving lipid metabolism of the present invention has an effect of suppressing weight gain and body fat accumulation, and reducing neutral lipids in blood and liver lipids.

[0011] The oil and fat composition of the present invention has a composition of triglyceride having SZU of 0.6 to 2.5 and C12 / U of 0.4 to 1.5 in the composition of fatty acids constituting the oil and fat composition of the present invention. The total number of triglycerides in which the total number of carbon atoms in the acyl group is 32 to 36 is less than 30%, and the total amount of triglycerides in which the total number of carbon atoms in the acyl group is 2 to 48 is 35% or more. is required.

[0012] When SZU is larger than 2.5 in the composition of the fatty acid constituting the target, it is difficult to obtain a remarkable effect such as suppression of weight gain. In particular, the effect of suppressing body fat accumulation cannot be obtained. If SZU is less than 0.6, no effect of reducing blood neutral lipid can be obtained.

[0013] Similarly, when C12ZU is greater than 1.5, it is difficult to obtain a remarkable effect such as suppression of weight gain. In particular, no effect of suppressing body fat accumulation can be obtained. When C12ZU is less than 0.4, the effect of reducing blood neutral lipid cannot be obtained.

[0014] Even when triglycerides having a total carbon number of 32 to 36 are 30% or more of the total, significant effects such as suppression of weight gain, etc., are difficult to obtain. In particular, no effect of suppressing body fat accumulation can be obtained.

[0015] On the other hand, even when the triglyceride having a total carbon number of 2 to 48 is less than 35% of the total, it is difficult to obtain a significant effect such as suppression of weight gain. In particular, no effect of suppressing body fat accumulation can be obtained. [0016] The oil and fat composition of the present invention can be obtained by fractionating palm kernel oil. The separation method is not limited to wet or dry separation, but dry separation without using a solvent or the like can be produced safely. In addition, it is preferable that the composition of the fatty acid and the triglyceride to be constituted can be easily within the range necessary for the oil and fat composition of the present invention.

[0017] Although the oil and fat composition of the present invention can be used as it is, the oil and fat composition of the present invention may be partially solidified at low temperatures, so depending on the application, it may be appropriately blended with liquid oil or the like. You can use it.

Example

[0018] The power of the present invention will be described in more detail below with reference to examples of the present invention. The spirit of the present invention is not limited to the following examples. In the examples, “%” and “part” mean weight standards.

[0019] Production Example 1

IV (iodine value) 23.4 Palm kernel olein was separated into liquid fraction (IV35.1, yield 41.7%) and crystal fraction (IV15.1, yield 58.3%) by dry fractionation. The liquid fraction (palm kernel double olein) was obtained as the low melting point fraction of palm kernel oil.

[0020] Production Example 2

After dissolving 1 part of coconut oil of IV 8.1 in 4 parts of acetone, cooling to 4 ° C and allowing the crystals to crystallize, hold for 30 minutes, then separate under reduced pressure into a crystal part and a filtrate part. . A liquid fraction (Isogo olein) was obtained as a low melting point fraction of coconut oil by solvent removal from the filtrate part (IV13.9, yield 40%)

[0021] Production Example 3

Using the palm kernel double olein obtained in Production Example 1, random transesterification with sodium methylate was carried out according to a conventional method.

[0022] Production Example 4

Medium chain fatty acid triglyceride MCT (Coconard MT—N, manufactured by Kao Corporation) and refined rapeseed oil are mixed at 1: 3: 87 (weight ratio), and random transesterification of this mixed oil is performed according to a conventional method. These palm kernel olein, palm kernel double olein, palm olein, palm kernel double olein random transesterified oil and fat, and the random transesterified oil obtained in Production Example 4 were purified by a conventional method. Their fatty acid composition and triglyceride composition are shown in Tables 1 and 2. From Table 1, SZU of palm kernel olein, palm kernel double olein, palm olein, palm kernel double olein random transesterified oil and fat and the random transesterified oil obtained in Production Example 4 are 3.42, 1.99 and 6. 75, 1.99 and 0.25, and C12ZU was 1.96, 1.04, 3.41, 1.04 and 0, respectively. In addition, from Table 2, C32 to 36 of palm kernel olein, palm kernel double olein, palm olein, palm kernel double olein random transesterified oil and the random transesterified oil obtained in Production Example 4 are 37.7% and 26. 9%, 59.8%, 18.4%, and 8.1%, and C42 to 48 are 30.4%, 40.6%, 14.4%, 47.7%, and 36.7%, respectively. It was. However, C32-36: Triglyceride in which the total carbon number of the acyl group is 32 to 36, C 42-48: Triglyceride in which the total carbon number of the acyl group is 42 to 48.

[0023] <Table 1>

[0024] Analysis conditions

Butyl esters were prepared by reaction of each oil sample with n-butanol using a sulfuric acid catalyst and analyzed under the following glc conditions. The obtained chromatogram data (% by weight as a ptyl ester) was expressed in terms of weight% as a methyl ester in terms of molecular weight.

glc analysis conditions

Sample: 1 ml hexane solution of 30 μ 1 of the butyl ester prepared above

Injection amount: 0.5 ^ 1

Equipment: GC—2010, manufactured by Shimadzu Corporation

First-class rams: Shinwa Kako Co., Ltd. ULBON HR— SS— 10 (0.25 mml. D. 50 m) Column temperature: 200 ° C, detector (FID) temperature: 250 ° C

[0025] <Table 2>

Content of triglycerides (C32 to C36 and C42 to 48)

[0026] Analysis conditions

Each oil sample was directly analyzed under the following glc conditions.

glc analysis conditions

Sample concentration: 1.5 ml hexane solution of fat 50 1

Injection volume: 1 1

Equipment: ヒュー 6850 Series GC system manufactured by Hewlett-Packard Company

Capillary column: Frontier Lab UA15W 0.15F (0.5 mml. D. 15m) Column temperature: 220 ° C to 12 ° C / min up to 350 ° C

Detector (FID) temperature: 350 ° C

Digestion and absorption test in mice (1)

Digestion and absorption tests were conducted in mice for 8 weeks using each refined oil of palm kernel olein, palm kernel double olein, palm olein and palm kernel double olein random transesterified oil obtained in Production Examples 1 to 3. It was. The mouse used was C57BLZ6J. After pre-breeding from 7 weeks of age to 1 week, 8 mice in each group were controlled with the soybean oil group in the mixed feed diet composition shown in Table 3 with a partial improvement in the AIN-93G composition. Breeding for weeks, body weight change 'feed efficiency • Body fat percentage was measured. The body fat percentage was measured using a PIXImus2 (GE Medical Systems), an X-ray bone density measuring device dedicated to experimental mice.

[0028] <Table 3> Diet composition (wt%)

[0029] Table 4 shows the results of a 56-day digestion and absorption test in mice. In the group using palm kernel double olein, about 12% suppression of body weight gain was observed compared to the soybean oil group as a control, and about 17% suppression was observed in body fat percentage, which was caused by excessive intake of fat. It was suggested that palm kernel double olein is effective as an anti-obesity oil.

[0030] <Table 4>

Rearing result

On the other hand, in the group using palm kernel olein, an increase of about 18% was observed in weight gain and an increase of about 9% in body fat percentage as compared with the soybean oil group as a control. . In the group using coconut olein, there was almost no change in weight gain compared to the soybean oil group, and an increase of about 6% in body fat percentage was observed. on the other hand, In the group using palm kernel doubleolein random transesterified oil and fat, body weight gain and body fat percentage were only about 8% and about 6% lower than the soybean oil group, respectively, and obesity as much as palm kernel doubleolein. It did not show a preventive effect. These results are summarized in Table 5.

[0032] <Table 5>

Comparison of the effect of each test oil group on soybean oil group

[0033] Further, after 56 days of breeding, the amounts of triglycerides in blood lipids and liver lipids after dissection in the control group and each test group were quantitatively analyzed, and the results are shown in Table 6. As shown in Table 7, which summarizes the effect of each test fat group on the rate of increase / decrease relative to the soybean oil group, only palm kernel doubleolein controls the amount of triglycerides in blood and liver lipids as a control. Significant suppression was seen for the soybean oil group.

[0034] <Table 6>

Triglyceride (TG) levels in blood and liver lipids Palm kernel olein Palm kernel double Soybean oil group

Use group Olean use group Blood TG (mgZdL) 103.7 7 ± 7. 6 95. 1 ± 5. 8 90. 9 ± 5. 1 Liver TG (mg / gLiver) 85. 2 ± 8. 1 85. 3 ± 7.8 56.4 ± 7.5

Zhuang Olaiin Palm nuclear double olein

Use group Random transesterified oil use group Blood TG (mgZdL) 99. 8 ± 7. 2 97. 8 ± 4.4

fl † TG (mg / gLiver 82. 2 ± 7. 3 80. 4 ± 9.1 [0035] <Table 7>

Increase / decrease rate of each test fat group with respect to soybean oil group

Digestion and absorption test in mice (2)

Breeding under the same conditions as in Example 1, using the refined oil of palm kernel double olein obtained in Production Example 1 and the random transesterified fat of MCT obtained in Production Example 4 as a control and soybean oil group Then, changes in body weight, feed efficiency, and body fat percentage were measured. However, each group had 6 animals and the breeding period was 111 days. The body fat percentage was measured before breeding, during breeding (bred for 56 days) and at the end of breeding (111 days). Further, Tables 8 and 10 show the results of quantitative analysis of blood lipids and post-dissection liver lipid triglyceride levels in mice of the control group and each test group after 111 days of breeding.

[0037] <Table 8>

Rearing result

[0038] <Table 9>

Triglyceride (TG) levels in blood and liver lipids Palm kernel double Oil of production example 4 Soybean oil group

Olein use group Use group Blood TG (mgZdL) 103.3 3 ± 9. 7 95. 8 ± 5. 9 124.0 ± 8.0 Liver TG (mgZgLiver) 180 ± 43 157 ± 29 138 ± 12

[0039] As shown in Table 9 and Table 11, which summarizes the effect as the rate of change of each test fat group with respect to the soybean oil group, palm kernel double olein has the same feed efficiency as in Production Example 4. Compared with the MCT and rapeseed oil random transesterified fats and oils obtained, the soybean oil group showed a greater suppression of body weight gain and body fat accumulation and a reduction in neutral lipids in the blood.

[0040] <Table 10>

Comparison of the effect of each test oil group on soybean oil group

[0041] <Table 11>

[0042] Finally, as shown in Table 12, the results of this experiment with soybean oil as a control are shown. In the composition of fatty acids, SZU is 0.6 to 2.5, and C12ZU is 0.4 to 1.5. Containing less than 30% of the total triglycerides having a total of 32 to 36 carbon atoms in the composition and containing 35% or more of the total triglycerides having a total carbon number of 2 to 48 for the acyl groups. Can reduce body weight gain, body fat accumulation, and neutral lipids in blood and liver lipids. [0043] <Table 12>

[0044] Explanation of symbols for various suppression reduction effects in Table 12 above

X · 'Inferior to the soybean group as a control.

△ · · 'There is little reduction effect compared to the soybean group as a control.

○ · · 'The reduction effect is clear compared with the soybean group as a control.

◎ · · · Significant reduction effect compared to soybean group as control.

The oil / fat composition that has shown all of these effects of reducing body weight gain, body fat accumulation, blood neutral lipid and liver neutral lipid, etc. It is only when it has.

High fat diet long-term feeding test in mice (3)

Using each refined oil of palm kernel olein and palm kernel double olein obtained in Production Example 1, a high-fat diet long-term feeding test in mice for 140 days was conducted. The mouse used was C57B L / 6J. After pre-breeding from 6 weeks of age to 1 week, AIN-93G composition was partially modified. The animals were reared for 140 days, and changes in body weight “weight of each organ” were measured for blood properties.

[0046] <Table 13>

Diet composition (wt%) Composition Soybean oil group Test oil and fat use group Soybean oil 30. 0 3. 0 Test oil and fat-27.0

Casein 20. 0 20. 0

Sucrose 1 3. 2 1 3. 2 β— corn starch 1 3. 75 1 3. 75

Οί— corn stsrch 1 3. 2 1 3. 2

L— Cystine 0. 3 0. 3 cellulose powder 5. 0 5. 0

Min.mix (AIN-93G) 3.5 5 3.5

Vit.mix (AIN-93G) 1. 0 1. 0

Choline bitartrate 0. 25 0. 25

[0047] Table 14 shows the results of a 140-day high-fat diet long-term feeding test in mice. In the group using palm kernel double violet, about 4.2% reduction in body weight was observed compared to the soybean oil group as a control.

[0048] <Table 14>

Rearing result

[0049] Furthermore, blood lipid levels were measured in mice of the control group and palm kernel double aurein group after 140 days of breeding, and the results are shown in Table 15. As shown in Table 15 which summarizes the effect as the rate of increase / decrease of the palm kernel double olein group with respect to the soybean oil group, the palm kernel double olein has the blood triglyceride content as compared with the soybean oil group as a control. Significant suppression was seen. Therefore, it was shown that the palm kernel double olein group has an effect of suppressing body weight and blood lipid even under high fat diet conditions.

[0050] <Table 15>

Increase / decrease rate of palm kernel doubleolein group relative to blood lipid level and soybean oil group (%)

Claims

The scope of the claims

[1] The composition of fatty acids is SZU of 0.6 to 2.5, C12ZU is 0.4 to 1.5, and the composition of triglycerides is composed of 32 to 36 carbon atoms in the asil group. An oil / fat composition for improving lipid metabolism, comprising less than 30% of a triglyceride and 35% or more of a triglyceride having a total of 42 to 48 carbon atoms in the acyl group. Where S: total saturated fatty acid content expressed as weight percent as methyl ester, U: total unsaturated fatty acid content expressed as weight percent as methyl ester.

[2] The fat composition for lipid metabolism improvement according to claim 1, obtained by fractionating palm kernel oil.

[3] The fat and oil composition for improving lipid metabolism according to claim 1 or 2, obtained by fractionating palm kernel oil a plurality of times.

[4] The fat composition for improving lipid metabolism according to claim 1 or 2, obtained by dry fractionating palm kernel oil.