JPWO2007086397A1 - Oil composition for improving lipid metabolism - Google Patents

Abstract

An object of the present invention is to provide an oil and fat composition for improving lipid metabolism, which has an improved safety and suppresses weight gain and body fat accumulation, and has a function of reducing neutral lipids in blood and liver lipids. S / U is 0.6 to 2.5 and C12 / U is 0.4 to 1.5 in the composition of the constituent fatty acid, and the total number of carbon atoms of the acyl group is 32 to 36 in the composition of the triglyceride that constitutes the fatty acid. The fat and oil composition for improving lipid metabolism, comprising less than 30% of the total triglyceride and 35% or more of the total triglyceride having the total acyl group carbon number of 42 to 48.

Description

The present invention relates to an oil and fat composition for improving lipid metabolism.

In recent years, with the Westernization of dietary habits, the intake of lipids has increased in the dietary habits of Japan, and the share of lipids in intake energy has reached three times that of 50 years ago. This is closely related to obesity and lifestyle-related diseases such as hyperlipidemia, diabetes and hypertension resulting from increased blood triglyceride (triglyceride) and cholesterol levels. It is thought to induce vascular disease. Because these are closely related to arteriosclerosis, keeping these values low is particularly important in terms of preventing lifestyle-related diseases, and not only the amount of food but also the importance of quality is important in preventing obesity It is reported. However, fats and oils are indispensable for the taste of food, and the fats and oils also have a cooking function as a heat medium, and there is a problem that it is not easy to reduce the intake.

For this reason, development of various functional foods and materials for the purpose of improving lipid metabolism has become active. Although the method of patent documents 1-4 is proposed and commercialized, all have the fault that it is hard to say that it is natural fats and oils. In particular, Patent Documents 1 to 3 disclose fat and oil compositions containing diglyceride as an active ingredient with little accumulation of body fat, but the safety of using fat and oil compositions rich in diglycerides as food is completely Is not proved to be.

Japanese Unexamined Patent Publication No. 4-300826 JP-A-8-60180 JP-A-10-176181 JP 2000-309794 A

An object of the present invention is to provide an oil and fat composition for improving lipid metabolism, which has an improved safety and suppresses weight gain and body fat accumulation, and has a function of reducing neutral lipids in blood and liver lipids.

As a result of intensive studies to solve the above problems, the present inventors have unexpectedly found that the ratio of saturated fatty acid mainly composed of lauric acid to long-chain monounsaturated fatty acid mainly composed of oleic acid is within a specific range. The present inventors have found that an oil and fat composition having a specific triglyceride composition has a function of suppressing weight gain and body fat accumulation, and a function of reducing neutral lipids in blood and liver lipids, thereby completing the present invention.

That is, the present invention
(1) S / U is 0.6 to 2.5 and C12 / U is 0.4 to 1.5 in the composition of the fatty acid to be configured, and the total carbon number of the acyl group is 32 in the composition of the triglyceride to be configured. An oil / fat composition for improving lipid metabolism, comprising ~ 36 triglycerides less than 30% of the total and 35% or more of triglycerides having a total of 42 to 48 carbon atoms in the acyl group. However, S: Total saturated fatty acid content expressed in weight% as methyl ester, U: Total unsaturated fatty acid content expressed in weight% as methyl ester.
(2) The fat and oil composition for improving lipid metabolism according to (1), obtained by fractionating palm kernel oil.
(3) The fat and oil composition for improving lipid metabolism according to (1) and (2), which is obtained by fractionating palm kernel oil a plurality of times.
(4) The fat and oil composition for improving lipid metabolism according to (1) and (2), obtained by dry-separating palm kernel oil.
It is.

As mentioned above, S / U is 0.6 to 2.5 in the composition of the constituent fatty acid, C12 / U is 0.4 to 1.5, and the total carbon number of the acyl group in the constituent of the constituent triglyceride The fat and oil composition for improving lipid metabolism, comprising less than 30% of triglycerides having a total of 32 to 36 and 35% or more of triglycerides having a total of 42 to 48 carbon atoms in the acyl group, Neutral lipids for body fat accumulation suppression and blood and liver lipids can be reduced.

The fat composition for improving lipid metabolism of the present invention can be widely used for foods usually containing fats and oils. For example, emulsified foods such as liquid coffee cream, margarine, mayonnaise, dressing, dairy products represented by potion cream, frozen confections such as ice cream, confectionery represented by chocolate, breads, and snack confectionery. Or added to processed meat products such as ham and sausage, fishery processed foods such as kamaboko and chikuwa, etc., without sacrificing flavor and texture.

The oil and fat composition for improving lipid metabolism of the present invention has an effect of suppressing weight gain and body fat accumulation, and reducing neutral lipids in blood and liver lipids.

The oil / fat composition of the present invention has an S / U of 0.6 to 2.5 and a C12 / U of 0.4 to 1.5 in the composition of the constituent fatty acid and the composition of the triglyceride to be formed. It is necessary that the total number of triglycerides in which the total number of carbon atoms in the acyl group is 32 to 36 is less than 30% and the total number of triglycerides in which the total number of carbon atoms in the acyl group is 42 to 48 is 35% or more. is there.

When S / U is larger than 2.5 in the composition of the constituent fatty acid, it is difficult to obtain a remarkable effect such as suppression of weight gain. In particular, the effect of suppressing body fat accumulation cannot be obtained. When S / U is less than 0.6, no effect of reducing blood neutral lipid is obtained.

Similarly, when C12 / U is larger than 1.5, it is difficult to obtain a remarkable effect such as suppression of weight gain. In particular, no effect of suppressing body fat accumulation can be obtained. When C12 / U is less than 0.4, the effect of reducing blood neutral lipid cannot be obtained.

Even when the triglyceride having a total carbon number of 32 to 36 is 30% or more of the total, it is difficult to obtain remarkable effects such as suppression of weight gain. In particular, no effect of suppressing body fat accumulation can be obtained.

On the other hand, when the total number of triglycerides having 42 to 48 carbon atoms is less than 35% of the total, it is difficult to obtain significant effects such as suppression of weight gain. In particular, no effect of suppressing body fat accumulation can be obtained.

The oil and fat composition of the present invention can be obtained by fractionating palm kernel oil. The separation method is not limited to wet and dry separation, but dry separation without using a solvent or the like can be produced safely. Further, it is preferable to fractionate a plurality of times from the viewpoint that the composition of the fatty acid and the triglyceride to be constituted can be easily within the range necessary for the oil and fat composition of the present invention.

The oil and fat composition of the present invention can be used as it is, but the oil and fat composition of the present invention may partially solidify at low temperatures, and may be used by appropriately blending with liquid oil or the like depending on the application. .

EXAMPLES The present invention will be described in more detail with reference to the following examples, but the spirit of the present invention is not limited to the following examples. In the examples, “%” and “part” mean weight basis.

Production Example 1
Palm kernel olein having an IV (iodine value) of 23.4 is fractionated into a liquid fraction (IV 35.1, yield 41.7%) and a crystalline fraction (IV 15.1, yield 58.3%) by dry fractionation. Thus, a liquid fraction (palm kernel double olein) was obtained as a low melting point fraction of palm kernel oil.

Production Example 2
After dissolving 1 part of IV 8.1 coconut oil in 4 parts of acetone, the mixture was cooled to −4 ° C., crystallized, held for 30 minutes, and then separated into a crystal part and a filtrate part by filtration under reduced pressure. By removing the solvent from the filtrate, a liquid fraction (coconut olein) was obtained as a low melting point fraction of coconut oil (IV 13.9, yield 40%).

Production Example 3
Using the palm kernel double olein obtained in Production Example 1, random transesterification with sodium methylate was carried out according to a conventional method.

Production Example 4
Medium chain fatty acid triglyceride MCT (Coconard MT-N, manufactured by Kao Co., Ltd.) and refined rapeseed oil were mixed at 13:87 (weight ratio), and the mixed oil was subjected to random transesterification according to a conventional method.
These palm kernel olein, palm kernel double olein, palm olein, palm kernel double olein random transesterified oil and fat and the random transesterified oil obtained in Production Example 4 were purified by conventional methods. Their fatty acid composition and triglyceride composition are shown in Tables 1 and 2. From Table 1, S / U of palm kernel olein, palm kernel double olein, palm olein, palm kernel double olein random transesterified fat and oil and random transesterified fat obtained in Production Example 4 is 3.42, 1.99, respectively. 6.75, 1.99 and 0.25 and C12 / U was 1.96, 1.04, 3.41, 1.04 and 0, respectively. Moreover, from Table 2, C32 to 36 of palm kernel olein, palm kernel double olein, palm olein, palm kernel double olein random transesterified oil and fat and random transesterified oil obtained in Production Example 4 are 37.7% and 26. 9%, 59.8%, 18.4% and 8.1%, C42-48 is 30.4%, 40.6%, 14.4%, 47.7% and 36.7% respectively. there were. However, C32-36: a triglyceride in which the total carbon number of the acyl group is 32-36, and C42-48: a triglyceride in which the total carbon number of the acyl group is 42-48.

<Table 1>

Analysis conditions A butyl ester was prepared by reaction of each fat sample with n-butanol using a sulfuric acid catalyst, and analyzed under the following glc conditions. The obtained chromatogram data (% by weight as butyl ester) was expressed in terms of% by weight as a methyl ester, in terms of molecular weight.
glc analysis condition sample: 30 μl of the above prepared butyl ester 1 ml hexane solution injection amount: 0.5 μl
Equipment: GC-2010 manufactured by Shimadzu Corporation
Capillary column: ULBON HR-SS-10 (0.25 mm ID 50 m) manufactured by Shinwa Kako Co., Ltd.
Column temperature: 200 ° C., detector (FID) temperature: 250 ° C.

<Table 2>
Content of triglycerides (C32 to C36 and C42 to 48)

Analysis conditions Each oil and fat sample was directly analyzed under the following glc conditions.
glc analysis condition Sample concentration: 1.5 μl hexane solution injection amount: 50 μl of fat / oil: 1 μl
Equipment: HP6850 series GC system capillary column manufactured by Hewlett-Packard Company: UA15W 0.15F (0.5 mm ID15 m) manufactured by Frontier Lab
Column temperature: from 220 ° C to 350 ° C at 12 ° C per minute Temperature rise detector (FID) temperature: 350 ° C

<Example 1>
Digestion and absorption test in mice (1)
Digestion and absorption tests were conducted in mice for 8 weeks using each refined oil of palm kernel olein, palm kernel double olein, palm olein and palm kernel double olein random transesterified oil obtained in Production Examples 1-3. The mouse used was C57BL / 6J, and after pre-breeding from 7 weeks of age to 1 week, the soybean oil group was controlled in the mixed feed diet composition of Table 3 in which the AIN-93G composition was partially improved. The animals were reared for 8 weeks, and changes in body weight, feed efficiency, and body fat percentage were measured. The body fat percentage was measured using a PIXImus2 (GE Medical Systems), an X-ray bone density measuring device dedicated to experimental mice.

<Table 3>
Diet composition (wt%)

Table 4 shows the results of a 56-day digestion and absorption test in mice. In the group using palm kernel double olein, about 12% suppression in body weight gain was observed compared to the soybean oil group as a control, and about 17% suppression in body fat percentage was observed. It was suggested that palm kernel double olein is effective as an anti-obesity oil.

<Table 4>
Rearing result

On the contrary, in the group using palm kernel olein, an increase of about 18% in body weight was observed and an increase in body fat percentage of about 9% was observed as compared with the soybean oil group as a control. . In the group using eggplant olein, there was almost no change in weight gain and an increase in body fat percentage of about 6% compared to the soybean oil group. On the other hand, in the group using palm kernel double olein random transesterified fats and oils, weight gain and body fat percentage are only about 8% and about 6% lower than the soybean oil group, respectively. Did not show the anti-obesity effect. These results are summarized in Table 5.

<Table 5>
Comparison of the effect of each test oil group on soybean oil group

Furthermore, the amount of triglycerides in blood lipids and liver lipids after dissection of the mice in the control group and each test group was quantitatively analyzed after feeding for 56 days. The results are shown in Table 6. As shown in Table 7, which summarizes the effect of each test fat group on the rate of increase / decrease relative to the soybean oil group, only palm kernel double olein increases the amount of triglycerides in blood and liver lipids as a control. Significant suppression was observed for the soybean oil group.

<Table 6>
Triglyceride (TG) levels in blood and liver lipids

<Table 7>
Increase / decrease rate of each test fat group with respect to soybean oil group

<Example 2>
Digestion and absorption test in mice (2)
Breeding under the same conditions as in Example 1 using the refined oil of palm kernel double olein obtained in Production Example 1 and the random transesterified oil of MCT oil obtained in Production Example 4 as a control for the soybean oil group Then, changes in body weight, feed efficiency, and body fat percentage were measured. However, each group had 6 animals and the breeding period was 111 days. The body fat percentage was measured before breeding, during breeding (bred for 56 days), and at the end of breeding (111 days). Further, Tables 8 and 10 show the results of quantitative analysis of the blood lipids and the liver lipids after dissection in the control group and each test group after 111 days of breeding.

<Table 8>
Rearing result

<Table 9>
Triglyceride (TG) levels in blood and liver lipids

As shown in Table 9 and Table 11, which summarize the effect as the rate of increase / decrease of each test fat group with respect to the soybean oil group, palm kernel double olein was obtained in Production Example 4 at an equivalent feed efficiency. Compared with the random transesterified oil and fat of MCT and rapeseed oil mixed oil, greater suppression of weight gain and body fat accumulation and reduction of neutral lipids in blood were observed in the soybean oil group.

<Table 10>
Comparison of the effect of each test oil group on soybean oil group

<Table 11>
Increase / decrease rate of each test fat group with respect to soybean oil group

Finally, as shown in Table 12, the results of this example with reference to soybean oil, S / U is 0.6 to 2.5 and C12 / U is 0.4 to 1.5. And the triglyceride in which the total number of carbon atoms in the acyl group is 32 to 36 in the composition of the triglyceride is less than 30% of the total and the total number of carbon atoms in the acyl group is 42 to 48. Those containing at least% can reduce body weight gain and body fat accumulation as well as neutral lipids in blood and liver lipids.

<Table 12>

Explanation of symbols for various suppression reduction effects in Table 12 above. Inferior to soybean group as control.
Δ: There is little reduction effect compared to the soybean group as a control.
○: The reduction effect is clear compared to the soybean group as a control.
◎ ・ ・ ・ Reduction effect is remarkable compared to the soybean group as a control.
The oil / fat composition that shows all these suppression-reducing effects at the same time, including weight gain suppression, body fat accumulation suppression, blood neutral lipid reduction and liver neutral lipid reduction, has the oil / fat composition defined in the present invention. Only if.

<Example 3>
High fat diet long-term feeding test in mice (3)
Using each refined oil of palm kernel olein and palm kernel double olein obtained in Production Example 1, a high fat diet long-term feeding test in mice for 140 days was performed. The mouse used was C57BL / 6J. After pre-breeding from 6 weeks of age to 1 week, the soybean oil group was controlled in the mixed feed diet composition of Table 13 in which the AIN-93G composition was partially improved. The mice were reared for 140 days, and changes in body weight, organ weights, and blood properties were measured.

<Table 13>
Diet composition (wt%)

Table 14 shows the results of a 140-day high-fat diet long-term feeding test in mice. In the group using palm kernel double olein, about 4.2% suppression of body weight gain was observed compared to the soybean oil group as a control.

<Table 14>
Rearing result

Furthermore, the blood lipid levels of the mice in the control group and palm kernel double olein group were measured after 140 days of breeding, and the results are shown in Table 15. As shown in Table 15 which summarizes the effect as the increase / decrease rate of the palm kernel double olein group with respect to the soybean oil group, the palm kernel double olein has the blood triglyceride content as compared with the soybean oil group as a control. Significant suppression was seen. Therefore, it was shown that the palm kernel double olein group has an effect of suppressing body weight and blood lipid even under high fat diet conditions.

<Table 15>
Increase / decrease rate of palm kernel double olein group in relation to blood lipid level and soybean oil group (%)

Claims (4)

S / U is 0.6 to 2.5, C12 / U is 0.4 to 1.5 in the composition of the fatty acid constituting, and the total carbon number of the acyl group is 32 to 36 in the composition of the triglyceride constituting The fat and oil composition for improving lipid metabolism, comprising less than 30% of the total triglyceride and 35% or more of the total triglyceride having the total acyl group carbon number of 42 to 48. However, S: Total saturated fatty acid content expressed in weight% as methyl ester, U: Total unsaturated fatty acid content expressed in weight% as methyl ester. The fat and oil composition for improving lipid metabolism according to claim 1, obtained by fractionating palm kernel oil. The fat and oil composition for improving lipid metabolism according to claim 1 or 2, which is obtained by fractionating palm kernel oil a plurality of times. The fat and oil composition for improving lipid metabolism according to claim 1, which is obtained by dry fractionating palm kernel oil.