WO2007079250A3 - Dispositifs et procedes d'enrichissement et de modification de cellules tumorales circulantes et d'autres particules - Google Patents

Dispositifs et procedes d'enrichissement et de modification de cellules tumorales circulantes et d'autres particules Download PDF

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Publication number
WO2007079250A3
WO2007079250A3 PCT/US2006/049634 US2006049634W WO2007079250A3 WO 2007079250 A3 WO2007079250 A3 WO 2007079250A3 US 2006049634 W US2006049634 W US 2006049634W WO 2007079250 A3 WO2007079250 A3 WO 2007079250A3
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WO
WIPO (PCT)
Prior art keywords
methods
particles
tumor cells
devices
circulating tumor
Prior art date
Application number
PCT/US2006/049634
Other languages
English (en)
Other versions
WO2007079250A2 (fr
Inventor
Martin Fuchs
Ying-Xin Wang
Yi-Shuian Huang
Neil X Krueger
Original Assignee
Cellpoint Diagnostics Inc
Martin Fuchs
Ying-Xin Wang
Yi-Shuian Huang
Neil X Krueger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/323,961 external-priority patent/US20070026416A1/en
Priority claimed from US11/324,108 external-priority patent/US20070026419A1/en
Priority claimed from US11/322,791 external-priority patent/US8921102B2/en
Application filed by Cellpoint Diagnostics Inc, Martin Fuchs, Ying-Xin Wang, Yi-Shuian Huang, Neil X Krueger filed Critical Cellpoint Diagnostics Inc
Publication of WO2007079250A2 publication Critical patent/WO2007079250A2/fr
Publication of WO2007079250A3 publication Critical patent/WO2007079250A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention se rapporte à des dispositifs et à des procédés permettant de détecter, d'enrichir et d'analyser des cellules tumorales circulantes et d'autres particules. L'invention a également trait à des méthodes permettant de diagnostiquer une maladie, par exemple un cancer, chez un sujet, par l'analyse d'un échantillon cellulaire provenant dudit sujet.
PCT/US2006/049634 2005-12-29 2006-12-29 Dispositifs et procedes d'enrichissement et de modification de cellules tumorales circulantes et d'autres particules WO2007079250A2 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US11/323,961 US20070026416A1 (en) 2005-07-29 2005-12-29 Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US11/323,961 2005-12-29
US11/324,108 US20070026419A1 (en) 2005-07-29 2005-12-29 Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US11/322,791 2005-12-29
US11/322,791 US8921102B2 (en) 2005-07-29 2005-12-29 Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US11/324,108 2005-12-29

Publications (2)

Publication Number Publication Date
WO2007079250A2 WO2007079250A2 (fr) 2007-07-12
WO2007079250A3 true WO2007079250A3 (fr) 2009-03-05

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2006/049634 WO2007079250A2 (fr) 2005-12-29 2006-12-29 Dispositifs et procedes d'enrichissement et de modification de cellules tumorales circulantes et d'autres particules
PCT/US2006/049582 WO2007079229A2 (fr) 2005-12-29 2006-12-29 Dispositifs et procedes d'enrichissement et de modification de cellules tumorales circulantes et d'autres particules

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2006/049582 WO2007079229A2 (fr) 2005-12-29 2006-12-29 Dispositifs et procedes d'enrichissement et de modification de cellules tumorales circulantes et d'autres particules

Country Status (1)

Country Link
WO (2) WO2007079250A2 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US9212977B2 (en) 2005-01-18 2015-12-15 Biocept, Inc. Cell separation using microchannel having patterned posts

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* Cited by examiner, † Cited by third party
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CN103382434B (zh) 2005-01-18 2016-05-25 生物概念股份有限公司 利用含排列成图案的立柱的微通道分离细胞
US8158410B2 (en) 2005-01-18 2012-04-17 Biocept, Inc. Recovery of rare cells using a microchannel apparatus with patterned posts
US7695956B2 (en) 2006-01-12 2010-04-13 Biocept, Inc. Device for cell separation and analysis and method of using
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2029779A4 (fr) 2006-06-14 2010-01-20 Living Microsystems Inc Utilisation de génotypage snp fortement parallèle pour diagnostic fétal
WO2013003624A2 (fr) 2011-06-29 2013-01-03 Academia Sinica Capture, purification et libération d'une substance biologique utilisant un revêtement de surface
US9494500B2 (en) 2012-10-29 2016-11-15 Academia Sinica Collection and concentration system for biologic substance of interest and use thereof
EP3608022A1 (fr) 2013-03-15 2020-02-12 The Trustees of Princeton University Procédés et dispositifs de purification à haut rendement
EP2971287B1 (fr) * 2013-03-15 2019-08-14 GPB Scientific, LLC Traitement microfluidique intégré de particules
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
WO2015153816A2 (fr) 2014-04-01 2015-10-08 Academia Sinica Procédés et systèmes pour le diagnostic et le pronostic du cancer
CN105381824B (zh) 2014-08-26 2019-04-23 中央研究院 收集器架构布局设计
US11009464B2 (en) * 2015-12-11 2021-05-18 International Business Machines Corporation Smartphone compatible on-chip biodetection using integrated optical component and microfluidic channel with nanopillar array
US10107726B2 (en) 2016-03-16 2018-10-23 Cellmax, Ltd. Collection of suspended cells using a transferable membrane
WO2017216270A1 (fr) 2016-06-15 2017-12-21 Grabmayr Heinrich Idenfication et quantification de molécules individuelles par la nanotechnologie en adn

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US6645731B2 (en) * 1998-02-12 2003-11-11 Immunivest Corporation Methods and reagents for the rapid and efficient isolation of circulating cancer cells
US20030234210A1 (en) * 2002-06-24 2003-12-25 Teragenics, Inc. Method and apparatus for sorting particles
US20040144651A1 (en) * 2002-10-23 2004-07-29 Huang Lotien Richard Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields
US6949355B2 (en) * 2001-10-11 2005-09-27 Aviva Biosciences Methods, compositions, and automated systems for separating rare cells from fluid samples

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US6770441B2 (en) * 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
GB0013658D0 (en) * 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
US20040005582A1 (en) * 2000-08-10 2004-01-08 Nanobiodynamics, Incorporated Biospecific desorption microflow systems and methods for studying biospecific interactions and their modulators
US20030036077A1 (en) * 2001-05-31 2003-02-20 Chronix Biomedical Methods for generating an mRNA expression profile from an ecellular mRNA containing blood sample and using the same to identify functional state markers

Patent Citations (4)

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US6645731B2 (en) * 1998-02-12 2003-11-11 Immunivest Corporation Methods and reagents for the rapid and efficient isolation of circulating cancer cells
US6949355B2 (en) * 2001-10-11 2005-09-27 Aviva Biosciences Methods, compositions, and automated systems for separating rare cells from fluid samples
US20030234210A1 (en) * 2002-06-24 2003-12-25 Teragenics, Inc. Method and apparatus for sorting particles
US20040144651A1 (en) * 2002-10-23 2004-07-29 Huang Lotien Richard Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9212977B2 (en) 2005-01-18 2015-12-15 Biocept, Inc. Cell separation using microchannel having patterned posts
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US9273355B2 (en) 2006-06-14 2016-03-01 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags
US8296076B2 (en) 2008-09-20 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuoploidy by sequencing
US8682594B2 (en) 2008-09-20 2014-03-25 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9353414B2 (en) 2008-09-20 2016-05-31 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9404157B2 (en) 2008-09-20 2016-08-02 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing

Also Published As

Publication number Publication date
WO2007079229A2 (fr) 2007-07-12
WO2007079229A3 (fr) 2009-01-22
WO2007079250A2 (fr) 2007-07-12

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