WO2007076302A3 - High throughput assay for modulators of peptidylarginine deiminase activity - Google Patents

High throughput assay for modulators of peptidylarginine deiminase activity Download PDF

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Publication number
WO2007076302A3
WO2007076302A3 PCT/US2006/062141 US2006062141W WO2007076302A3 WO 2007076302 A3 WO2007076302 A3 WO 2007076302A3 US 2006062141 W US2006062141 W US 2006062141W WO 2007076302 A3 WO2007076302 A3 WO 2007076302A3
Authority
WO
WIPO (PCT)
Prior art keywords
modulators
high throughput
throughput assay
peptidylarginine deiminase
deiminase activity
Prior art date
Application number
PCT/US2006/062141
Other languages
French (fr)
Other versions
WO2007076302A2 (en
Inventor
Brian Glenn Werneburg
Martha Priscilla Brown
Dorothy Mae Freeman
Jeffrey David Yingling
Original Assignee
Boehringer Ingelheim Int
Boehringer Ingelheim Pharma
Brian Glenn Werneburg
Martha Priscilla Brown
Dorothy Mae Freeman
Jeffrey David Yingling
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int, Boehringer Ingelheim Pharma, Brian Glenn Werneburg, Martha Priscilla Brown, Dorothy Mae Freeman, Jeffrey David Yingling filed Critical Boehringer Ingelheim Int
Publication of WO2007076302A2 publication Critical patent/WO2007076302A2/en
Publication of WO2007076302A3 publication Critical patent/WO2007076302A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

The present invention is a high through-put assay for PAD activity. The assay is designed to identify modulators of PAD catalysis for the treatment of multiple sclerosis and rheumatoid arthritis.
PCT/US2006/062141 2005-12-20 2006-12-15 High throughput assay for modulators of peptidylarginine deiminase activity WO2007076302A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US74305405P 2005-12-20 2005-12-20
US60/743,054 2005-12-20

Publications (2)

Publication Number Publication Date
WO2007076302A2 WO2007076302A2 (en) 2007-07-05
WO2007076302A3 true WO2007076302A3 (en) 2008-04-03

Family

ID=38191069

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/062141 WO2007076302A2 (en) 2005-12-20 2006-12-15 High throughput assay for modulators of peptidylarginine deiminase activity

Country Status (1)

Country Link
WO (1) WO2007076302A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0915336A2 (en) 2008-06-16 2017-06-27 Academisch Ziekenhuis Leiden compound capable of inhibiting the activity of a pad, composition, and use of a compound
ES2445940T1 (en) 2011-03-25 2014-03-06 Almac Sciences (Scotland) Limited Enzymatic assays
CN110426514B (en) * 2019-08-28 2023-05-12 苏州新格诺康生物技术有限公司 Method for detecting activity of peptide acyl arginine deiminase (PAD)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1275733A2 (en) * 2001-07-13 2003-01-15 Pfizer Limited Assay methods for peptidase modulators

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1275733A2 (en) * 2001-07-13 2003-01-15 Pfizer Limited Assay methods for peptidase modulators

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ROTHNAGEL J A ET AL: "Citrulline in proteins from the enzymatic deimination of arginine residues.", METHODS IN ENZYMOLOGY 1984, vol. 107, 1984, pages 624 - 631, XP009086183, ISSN: 0076-6879 *
TAKAHARA H ET AL: "SPECIFIC MODIFICATION OF THE FUNCTIONAL ARGININE RESIDUE IN SOYBEAN TRYPSIN INHIBITOR KUNITZ BY PEPTIDYLARGININE DEIMINASE", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, no. 14, 1985, pages 8378 - 8383, XP002440995, ISSN: 0021-9258 *
VOSSENAAR E R ET AL: "PAD, A GROWING FAMILY OF CITRULLINATING ENZYMES: GENES, FEATURES AND INVOLVEMENT IN DISEASE", BIOESSAYS, CAMBRIDGE, GB, vol. 25, no. 11, 17 October 2003 (2003-10-17), pages 1106 - 1118, XP008057229, ISSN: 0265-9247 *

Also Published As

Publication number Publication date
WO2007076302A2 (en) 2007-07-05

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