WO2007061848A3 - Methods for producing, growing, and preserving listeria vaccine vectors - Google Patents

Methods for producing, growing, and preserving listeria vaccine vectors Download PDF

Info

Publication number
WO2007061848A3
WO2007061848A3 PCT/US2006/044681 US2006044681W WO2007061848A3 WO 2007061848 A3 WO2007061848 A3 WO 2007061848A3 US 2006044681 W US2006044681 W US 2006044681W WO 2007061848 A3 WO2007061848 A3 WO 2007061848A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
producing
preserving
growing
vaccine vectors
Prior art date
Application number
PCT/US2006/044681
Other languages
French (fr)
Other versions
WO2007061848A2 (en
Inventor
John Rothman
Yvonne Paterson
Thorsten Verch
Amanda Weiss
Philip Bassett
Original Assignee
Univ Pennsylvania
Advaxis
John Rothman
Yvonne Paterson
Thorsten Verch
Amanda Weiss
Philip Bassett
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Pennsylvania, Advaxis, John Rothman, Yvonne Paterson, Thorsten Verch, Amanda Weiss, Philip Bassett filed Critical Univ Pennsylvania
Publication of WO2007061848A2 publication Critical patent/WO2007061848A2/en
Publication of WO2007061848A3 publication Critical patent/WO2007061848A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/0208—Specific bacteria not otherwise provided for
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/523—Bacterial cells; Fungal cells; Protozoal cells expressing foreign proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
    • G01N2333/39—Assays involving biological materials from specific organisms or of a specific nature from fungi from yeasts
    • G01N2333/40—Assays involving biological materials from specific organisms or of a specific nature from fungi from yeasts from Candida

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides methods for cryopreservation and lyophilization of a Listeria strain, methods for producing a cell bank or a batch of vaccine doses of same, methods of characterizing same, and defined microbiological media.
PCT/US2006/044681 2005-11-17 2006-11-16 Methods for producing, growing, and preserving listeria vaccine vectors WO2007061848A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US73741905P 2005-11-17 2005-11-17
US60/737,419 2005-11-17
US75819406P 2006-01-12 2006-01-12
US60/758,194 2006-01-12

Publications (2)

Publication Number Publication Date
WO2007061848A2 WO2007061848A2 (en) 2007-05-31
WO2007061848A3 true WO2007061848A3 (en) 2007-09-13

Family

ID=38067780

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/044681 WO2007061848A2 (en) 2005-11-17 2006-11-16 Methods for producing, growing, and preserving listeria vaccine vectors

Country Status (1)

Country Link
WO (1) WO2007061848A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9226958B2 (en) 2010-10-01 2016-01-05 University Of Georgia Research Foundation, Inc. Use of Listeria vaccine vectors to reverse vaccine unresponsiveness in parasitically infected individuals
US9463227B2 (en) 2011-03-11 2016-10-11 Advaxis, Inc. Listeria-based adjuvants

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8114414B2 (en) 1994-11-08 2012-02-14 The Trustees Of The University Of Pennsylvania Compositions and methods for treatment of cervical cancer
US7635479B2 (en) 2000-03-29 2009-12-22 The Trustees Of The University Of Pennsylvania Composition and methods for enhancing immunogenecity of antigens
US8791237B2 (en) 1994-11-08 2014-07-29 The Trustees Of The University Of Pennsylvania Compositions and methods for treatment of non-hodgkins lymphoma
US8956621B2 (en) 1994-11-08 2015-02-17 The Trustees Of The University Of Pennsylvania Compositions and methods for treatment of cervical dysplasia
US9012141B2 (en) 2000-03-27 2015-04-21 Advaxis, Inc. Compositions and methods comprising KLK3 of FOLH1 antigen
US7700344B2 (en) 2001-03-26 2010-04-20 The Trustees Of The University Of Pennsylvania Compositions and methods for enhancing the immunogenicity of antigens
US8771702B2 (en) 2001-03-26 2014-07-08 The Trustees Of The University Of Pennsylvania Non-hemolytic LLO fusion proteins and methods of utilizing same
US8268326B2 (en) 2006-08-15 2012-09-18 The Trustees Of The University Of Pennsylvania Compositions comprising HMW-MAA and fragments thereof, and methods of use thereof
WO2008079172A2 (en) 2006-08-15 2008-07-03 The Trustees Of The University Of Pennsylvania Compositions comprising hmw-maa and fragments thereof, and methods of use thereof
EP2853269B1 (en) 2008-05-19 2019-05-01 Advaxis, Inc. Dual delivery system for heterologous antigens comprising a recombinant Listeria strain attenuated by mutation of dal/dat and deletion of ActA comprising a nucleic acid molecule encoding an listeriolysin O - prostate specific anigen fusion protein
US9650639B2 (en) 2008-05-19 2017-05-16 Advaxis, Inc. Dual delivery system for heterologous antigens
JP5539411B2 (en) 2009-03-04 2014-07-02 ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア Compositions containing angiogenic factors and methods of use thereof
JP5985397B2 (en) 2009-11-11 2016-09-06 アドバクシス インコーポレイテッド Recombinant Listeria strain and immunogenic composition containing the same
US10016617B2 (en) 2009-11-11 2018-07-10 The Trustees Of The University Of Pennsylvania Combination immuno therapy and radiotherapy for the treatment of Her-2-positive cancers
WO2012125551A1 (en) 2011-03-11 2012-09-20 Advaxis Listeria-based adjuvants
US10119131B2 (en) * 2011-03-16 2018-11-06 Biospecifics Technologies Corp. Compositions and methods for producing clostridial collagenases
CN104411327A (en) 2012-03-12 2015-03-11 阿德瓦希斯公司 Suppressor cell function inhibition following listeria vaccine treatment
CA2998857A1 (en) * 2015-09-15 2017-03-23 Anu Wallecha Manufacturing method of an immunotherapeutic formulation comprising a recombinant listeria strain
US20180325964A1 (en) * 2015-11-20 2018-11-15 Advaxis, Inc. Manufacturing device and method of an immunotherapeutic formulation comprising a recombinant listeria strain
CN105506054A (en) * 2016-02-05 2016-04-20 夏淑平 Listeria monocytogenes selective medium
CN105506055A (en) * 2016-02-05 2016-04-20 夏淑平 Selective medium for listeria monocytogenes of stool sample
SG11201901979SA (en) * 2016-11-30 2019-04-29 Advaxis Inc Immunogenic compositions targeting recurrent cancer mutations and methods of use thereof
MX2020003100A (en) 2017-09-19 2020-08-20 Advaxis Inc COMPOSITIONS AND METHODS FOR LYOPHILIZATION OF BACTERIA OR <i>LISTERIA </i>STRAINS.

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830702A (en) * 1990-10-31 1998-11-03 The Trustees Of The University Of Pennsylvania Live, recombinant listeria monocytogenes and production of cytotoxic T-cell response
US20060121053A1 (en) * 2004-10-18 2006-06-08 Pamela Sweeney High cell density process for growth of Listeria

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830702A (en) * 1990-10-31 1998-11-03 The Trustees Of The University Of Pennsylvania Live, recombinant listeria monocytogenes and production of cytotoxic T-cell response
US20060121053A1 (en) * 2004-10-18 2006-06-08 Pamela Sweeney High cell density process for growth of Listeria

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AVIS K. ET AL.: "Cryopreservatin Application in Pharmaceuticals and Biotechnology. Drug Manufacturing and Technology Series", vol. 5, 1999, COLORADO, article WAGNER ET AL.: "Method selection considerations for long-term preservation of mammalian cells and microorganisms", pages: 315 - 359 *
EL-KEST ET AL.: "Injury and Death of Frozen Listeria Monocytogenes as Affected by Glycerol and Milk Components?", J. DAIRY SCIENCE, vol. 74, 1991, pages 1201 - 1208 *
GROUP HODGSON. LISTERIA METHODS, 2000, Retrieved from the Internet <URL:http://www.template.bio.warwick.ac.uk/staff/hodgson/listeria/methods.html> *
KATHARIOU S.: "Tn916-Induced Mutations in the Hemolysin Determinant Affecting Virulence of Listeria monocytogenes", JOURNAL OF BACTERIOLOGY, March 1987 (1987-03-01), pages 1291 - 1297 *
KUHN M. ET AL.: "Hemolysin Supports Survival But Not Entry of the Intracellular Bacterium Listeria Monocytogenes", INFECTION AND IMMUNITY, January 1988 (1988-01-01) *
SAMBROOK ET AL.: "Molecular Cloning A Laboratory Manual", COLD SPRING HARBOUR LABORATORY PRESS, vol. 3, 1989, pages A.5 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9226958B2 (en) 2010-10-01 2016-01-05 University Of Georgia Research Foundation, Inc. Use of Listeria vaccine vectors to reverse vaccine unresponsiveness in parasitically infected individuals
US9463227B2 (en) 2011-03-11 2016-10-11 Advaxis, Inc. Listeria-based adjuvants

Also Published As

Publication number Publication date
WO2007061848A2 (en) 2007-05-31

Similar Documents

Publication Publication Date Title
WO2007061848A3 (en) Methods for producing, growing, and preserving listeria vaccine vectors
WO2006046148A3 (en) Rna constructs
WO2007024756A3 (en) Reduced genome e. coli
WO2007098150A3 (en) Photobioreactor and uses therefor
WO2007059084A3 (en) Methods for preparing cord matrix stem cells (cmsc) for long term storage and for preparing a segment of umbilical cord for cryopreservation
MY183385A (en) Production of polio virus at high titers for vaccine production
WO2010120515A3 (en) Methods for producing cultured pearls in conch and other gastropods
WO2007050498A3 (en) Methods of protein production using anti-senescence compounds
WO2006102163A3 (en) Transfection reagents for non-adherent suspension cells
WO2007067044A3 (en) Modified active-ingredient-containing pellets/capsules
WO2007137075A3 (en) Use of non-agrobacterium bacterial species for plant transformation
IL189188A (en) Phenylamidine derivatives, process for their preparation and methods for controlling phytopatogenic fungi or damaging insects
WO2011100477A3 (en) Antibodies and processes for preparing the same
WO2009139930A3 (en) Antibodies and processes for preparing the same
WO2006063053A3 (en) Methods of producing influenza vaccine compositions
WO2009126470A3 (en) Plant regulatory elements and uses thereof
WO2018053451A8 (en) Novel paratransgenic system for the biocontrol of disease-transmitting mosquitos
WO2006132944A3 (en) Probiotic system for aquaculture
WO2008074418A3 (en) Bisoximes as fungicides and arthropodicides
WO2012131495A3 (en) Mutant bacillus thuringiensis cry genes and methods of use
WO2008122550A3 (en) Pulverizer and corresponding method for preparing a biological sample for processing
WO2008033556A3 (en) High pressure treatment of proteins for reduced immunogenicity
WO2005092924A3 (en) Rab9a, rab11a, and modulators thereof related to infectious disease
CY1112746T1 (en) CRYPTOLOGY OF AMBULANCES
WO2011031145A3 (en) Improved soy milk fermentation

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 06837912

Country of ref document: EP

Kind code of ref document: A2