WO2007056312A3 - Universal reporter tag for nucleozyme assays - Google Patents

Universal reporter tag for nucleozyme assays Download PDF

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Publication number
WO2007056312A3
WO2007056312A3 PCT/US2006/043236 US2006043236W WO2007056312A3 WO 2007056312 A3 WO2007056312 A3 WO 2007056312A3 US 2006043236 W US2006043236 W US 2006043236W WO 2007056312 A3 WO2007056312 A3 WO 2007056312A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleozyme
assays
reporter tag
universal reporter
universal
Prior art date
Application number
PCT/US2006/043236
Other languages
French (fr)
Other versions
WO2007056312A2 (en
Inventor
Cynthia Chang
Nikoliai Kalnine
Original Assignee
Clontech Lab Inc
Cynthia Chang
Nikoliai Kalnine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clontech Lab Inc, Cynthia Chang, Nikoliai Kalnine filed Critical Clontech Lab Inc
Publication of WO2007056312A2 publication Critical patent/WO2007056312A2/en
Publication of WO2007056312A3 publication Critical patent/WO2007056312A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods and compositions for detecting a target nucleic acid in a sample are provided. Aspects of the invention include the use of a universal nucleozyme tag. The invention further provides kits and systems for practicing the subject methods.
PCT/US2006/043236 2005-11-07 2006-11-06 Universal reporter tag for nucleozyme assays WO2007056312A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US73443205P 2005-11-07 2005-11-07
US60/734,432 2005-11-07
US73714705P 2005-11-15 2005-11-15
US60/737,147 2005-11-15

Publications (2)

Publication Number Publication Date
WO2007056312A2 WO2007056312A2 (en) 2007-05-18
WO2007056312A3 true WO2007056312A3 (en) 2009-05-07

Family

ID=38023904

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/043236 WO2007056312A2 (en) 2005-11-07 2006-11-06 Universal reporter tag for nucleozyme assays

Country Status (1)

Country Link
WO (1) WO2007056312A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999045146A1 (en) * 1998-03-05 1999-09-10 Johnson & Johnson Research Pty. Ltd. Zymogenic nucleic acid detection methods, and related molecules and kits
US6818420B2 (en) * 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999045146A1 (en) * 1998-03-05 1999-09-10 Johnson & Johnson Research Pty. Ltd. Zymogenic nucleic acid detection methods, and related molecules and kits
US6818420B2 (en) * 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same

Also Published As

Publication number Publication date
WO2007056312A2 (en) 2007-05-18

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