WO2006133758A3 - Vorrichtung zur anreicherung/abtrennung von nicht-methylierte cpg-motive enthaltender dna - Google Patents

Vorrichtung zur anreicherung/abtrennung von nicht-methylierte cpg-motive enthaltender dna Download PDF

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Publication number
WO2006133758A3
WO2006133758A3 PCT/EP2006/003328 EP2006003328W WO2006133758A3 WO 2006133758 A3 WO2006133758 A3 WO 2006133758A3 EP 2006003328 W EP2006003328 W EP 2006003328W WO 2006133758 A3 WO2006133758 A3 WO 2006133758A3
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WO
WIPO (PCT)
Prior art keywords
unmethylated cpg
enriching
dna containing
separating dna
containing unmethylated
Prior art date
Application number
PCT/EP2006/003328
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English (en)
French (fr)
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WO2006133758A2 (de
Inventor
Eberhard Straube
Stefan Russwurm
Marc Lehmann
Original Assignee
Sirs Lab Gmbh
Eberhard Straube
Stefan Russwurm
Marc Lehmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sirs Lab Gmbh, Eberhard Straube, Stefan Russwurm, Marc Lehmann filed Critical Sirs Lab Gmbh
Publication of WO2006133758A2 publication Critical patent/WO2006133758A2/de
Publication of WO2006133758A3 publication Critical patent/WO2006133758A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)

Abstract

Es wird eine Vorrichtung zur Anreicherung und/oder Abtrennung von nicht-methylierte CpG-Motive enthaltender DNA bereitgestellt, umfassend a) ein Reaktionsgefäß mit mindestens einer Öffnung und b) ein Protein, das nicht-methylierte CpG-Motive über eine Bindungsstelle spezifisch erkennt, wobei das Protein eine 25%ige bis 35%ige Homologie zum Wildtyp-CGPB-Protein aufweist und gegenüber diesem verkürzt ist, wobei sich das Protein innerhalb des Reaktionsgefäßes befindet.
PCT/EP2006/003328 2005-06-16 2006-04-11 Vorrichtung zur anreicherung/abtrennung von nicht-methylierte cpg-motive enthaltender dna WO2006133758A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE202005009490.0 2005-06-16
DE200520009490 DE202005009490U1 (de) 2005-06-16 2005-06-16 Vorrichtung zur Anreicherung/Abtrennung von nicht-methylierte CpG-Motive enthaltender DNA

Publications (2)

Publication Number Publication Date
WO2006133758A2 WO2006133758A2 (de) 2006-12-21
WO2006133758A3 true WO2006133758A3 (de) 2007-03-01

Family

ID=37057242

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/003328 WO2006133758A2 (de) 2005-06-16 2006-04-11 Vorrichtung zur anreicherung/abtrennung von nicht-methylierte cpg-motive enthaltender dna

Country Status (2)

Country Link
DE (1) DE202005009490U1 (de)
WO (1) WO2006133758A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2046818A4 (de) * 2006-07-11 2010-03-03 Leap Biosciences Corp Verfahren für selektive proteinanreicherung und entsprechende anwendungen
DE102007041864B4 (de) 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
DE102012219142B4 (de) * 2012-10-19 2016-02-25 Analytik Jena Ag Verfahren zum Trennen, Erfassen oder Anreichern von unterschiedlichen DNA-Spezies

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004033683A1 (de) * 2002-09-18 2004-04-22 Sirs-Lab Gmbh Verfahren zur anreicherung prokaryontischer dna
WO2005085440A1 (de) * 2004-03-05 2005-09-15 Sirs-Lab Gmbh VERFAHREN ZUR ANREICHERUNG UND/ODER ABTRENNUNG VON PROKARYONTER DNA MITTELS EINES PROTEINS, DAS NICHT-METHYLIERTE CpG-MOTIVE ENTHALTENDE DNA SPEZIFISCH BINDET
DE102004010928A1 (de) * 2004-03-05 2005-09-22 Sirs-Lab Gmbh Protein zur Bindung prokaryonter DNA sowie Verfahren zur Trennung und Anreicherung prokaryonter DNA

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004033683A1 (de) * 2002-09-18 2004-04-22 Sirs-Lab Gmbh Verfahren zur anreicherung prokaryontischer dna
WO2005085440A1 (de) * 2004-03-05 2005-09-15 Sirs-Lab Gmbh VERFAHREN ZUR ANREICHERUNG UND/ODER ABTRENNUNG VON PROKARYONTER DNA MITTELS EINES PROTEINS, DAS NICHT-METHYLIERTE CpG-MOTIVE ENTHALTENDE DNA SPEZIFISCH BINDET
DE102004010928A1 (de) * 2004-03-05 2005-09-22 Sirs-Lab Gmbh Protein zur Bindung prokaryonter DNA sowie Verfahren zur Trennung und Anreicherung prokaryonter DNA

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] EBI; 9 March 2000 (2000-03-09), "CGBP", XP002404715, retrieved from EMBL Database accession no. AF149758 *
VOO KUI SHIN ET AL: "Cloning of a mammalian transcriptional activator that binds unmethylated CpG motifs and shares a CXXC domain with DNA methyltransferase, human trithorax, and methyl-CpG binding domain protein 1", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 20, no. 6, March 2000 (2000-03-01), pages 2108 - 2121, XP002262527, ISSN: 0270-7306 *

Also Published As

Publication number Publication date
WO2006133758A2 (de) 2006-12-21
DE202005009490U1 (de) 2006-10-19

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