WO2006115079A1

WO2006115079A1 - Thermal lens spectrum analysis system and thermal lens signal correction method

Info

Publication number: WO2006115079A1
Application number: PCT/JP2006/307889
Authority: WO
Inventors: Jun Yamaguchi; Takashi Fukuzawa
Original assignee: Nippon Sheet Glass Company, Limited
Priority date: 2005-04-20
Filing date: 2006-04-07
Publication date: 2006-11-02
Also published as: JP2006300721A; JP4145892B2

Abstract

There are provided a thermal lens spectrum analysis system and a thermal lens signal correction method capable of accurately measuring a sample even if the thermal lens signal intensity is changed by an external environment change. The thermal lens spectrum analysis system (10) includes: a micro chemical chip (2) having a groove (1) into which a sample-in-liquid has been introduced; a refractivity distribution type rod lens (3) for converging excitation light propagating from a light source unit (7) via an optical fiber (5) and detection light into the sample-in-liquid so as to generate a thermal lens signal; a photo-electric converter (22) for detecting the light quantity of the excitation light and the detection light and the thermal lens signal intensity; and a personal computer (25) for correcting the measurement value of the thermal lens signal intensity by accumulating the thermal lens signal intensity measurement values, (a predetermined light quantity of excitation light/a measured light quantity of excitation light), and/or a second radio (a predetermined light quantity of detection light/ a measured light quantity of detection light).

Description

MEANS TO USE THERMAL LENS SPECTRUM ANALYSIS SYSTEM AND THERMAL LENS SIGNAL CORRECTION METHOD

Technical field

The present invention relates to a thermal lens spectroscopic analysis system and a thermal lens signal correction method. Background art

Conventionally, integration technology for conducting chemical reactions in a minute space has attracted attention from the viewpoints of speeding up chemical reactions, reaction in small amounts, on-site analysis, etc. ing.

One such integration technology is a thermal lens spectroscopic system that uses a microchemical chip to mix, react, separate, extract, and detect liquid samples.

For example, as shown in FIG. 4, the thermal lens spectroscopic analysis system 100 has a plate-like member 1 2 0 with a flow path in which a sample in liquid is filled, and a plate-like member 1 with a flow path. The liquid in the flow path of the plate-like member 1 2 0 with a flow path is connected to the optical fiber with a lens 1 0 0 and the optical fiber 1 0 0 with a lens that is disposed above the 2 0 and has a lens attached to the tip. A light source unit 110 for irradiating a medium sample with excitation light and irradiating detection light to a thermal lens generated in the liquid sample by the irradiated excitation light; and a plate-like member with flow path 120 And a detection device 1300 which is arranged below and detects detection light via a thermal lens generated in the sample in liquid in the flow path of the plate-like member 120 with flow path by excitation light.

The optical fiber with lens 1 0 0 has a lens 1 0 2 and one end of the lens 1 0 2 is connected to the light source unit 1 1 0 and the other end is connected to the light source unit 1 1 0 1 and the optical fiber 1 1 0 1 is fixed to the optical fiber 1 1 0 1 via the ferrule 1 0 3 and force It consists of

The light source unit 1 1 0 is connected to the excitation light source 1 0 5 that outputs the excitation light and the excitation light source 1 0 5 and modulates the excitation light output from the excitation light source 1 0 5. A modulator 1 0 7, a detection light source 1 0 6 that outputs detection light, an excitation light source 1 0 5 and a detection light source 1 0 6 via optical fibers 1 1 4, respectively. In addition, it is connected to the optical fiber 1 0 1 of the optical fiber 10 0 with a lens, and combines the excitation light output from the excitation light source 1 0 5 and the detection light output from the detection light source 1 0 6. And an optical multiplexer 1 0 8 for making these pumping light and detection light incident on the optical fiber 1 0 1 enter, respectively.

The plate-like member with a channel 1 2 0 is composed of an upper glass substrate 2 0 1, a middle glass substrate 2 0 2, and a lower glass substrate 2 which are laminated and adhered in three layers in order from the optical fiber 1 0 0 with lens. 0 consists of three. The middle glass substrate 20 2, which is the middle layer of the plate-like member 1 2 0 with a flow path, is mixed with a sample in liquid, stirred, synthesized, separated, extracted by a thermal lens spectroscopic analysis system 1 0 0 0 And a flow path 20 4 through which the sample in the liquid flows during operation such as detection.

The detection device 1 30 is located at a position facing the flow path 20 4 of the plate-like member 1 2 0 with flow path with a predetermined interval and facing the optical fiber with lens 1 100. A wavelength filter 4 0 2 that separates the combined excitation light and detection light and selectively transmits only the detection light, and a channel 2 0 4 below the wavelength filter 1 4 0 2. And a computer connected to the photoelectric converter 4 0 1 via a plug-in amplifier 4 0 4 for detecting the detection light disposed at a position facing the 2 4 0 5 and force (for example, see Japanese Laid-Open Patent Publication No. 2 0 2-3 6 5 2 52). In this thermal lens spectroscopic analysis system 1 0 0 0, the excitation light and detection light output from the excitation light source 1 0 5 and detection light source 1 0 6 are affected by changes in the external environment such as temperature change. Lens 1 0 2 due to changes in the amount of light, changes in the loss of optical multiplexer 10 0 8, expansion of the stage (not shown) on which thermal lens spectroscopic analysis system 1 0 0 0 is mounted, etc. Misalignment between the lens and the plate-like member with flow path 120 and misalignment between the lens 10 0 2 and the photoelectric converter 4 0 1 occurred.

As a result, the amount of excitation light incident on the sample in the liquid in the flow path 20 4 to form a thermal lens changes, or the amount of detection light incident on the photoelectric converter 4 0 1 changes. Sometimes.

In addition, since the thermal lens spectroscopic method executed by the thermal lens spectroscopic analysis system 100 is usually an analog measurement, the excitation light incident on the liquid sample in the channel 20 4 described above is used. The change in the amount of light and the change in the amount of the detection light incident on the photoelectric converter 4 0 1 appear as a change in the intensity of the thermal lens signal finally measured, and the reproducibility in the measurement of the thermal lens signal intensity is reduced. It was.

In order to improve the reproducibility of thermal lens signal intensity measurement, electronic components such as Peltier elements are added to the light source unit 110 to control the temperature, and the measurement environment of the thermal lens spectroscopic analysis system 10 00 is controlled. It may be possible to remove changes in the external environment such as temperature changes that are the root cause of the decrease in reproducibility in thermal lens signal intensity measurement by strictly controlling the temperature, but in this case, electronic components such as Peltier elements There are problems such as increasing the size and cost of the thermal lens spectroscopic analysis system due to the addition of 100, and lack of versatility in the measurement environment.

Therefore, the thermal lens signal is changed in accordance with the change in the amount of excitation light incident on the sample in the liquid in the channel 20 4 and the change in the amount of detection light incident on the photoelectric converter 4 0 1. It may be considered to correct the intensity. There has already been disclosed a radiant heat sensor that corrects the amount of laser light measured by using two photosensitive detectors (for example, US Pat. No. 5,51,13). 0 0 6). In a spatial optical system that does not use an optical fiber, such as this radiant heat sensor, the light can be branched by a filter installed in the middle of the optical path. The amount of light can be easily corrected.

However, in a thermal lens spectroscopic analysis system that uses an optical fiber, the light is confined within the optical fiber, so a special branch module is required to split the light, making branching easier. Cannot be detected. In particular, branch modules that split light in the visible region are often susceptible to temperature changes and the light branch ratio varies. Therefore, in the branch light detection for correcting the thermal lens signal intensity, it is possible to distinguish whether the change in the light branch ratio is detected or the change in the light amount of the excitation light and the detection light is detected. I can't.

In addition, since the thermal lens spectroscopic analysis system uses two types of light, excitation light and detection light, at least two detectors are required to detect the amount of excitation light and detection light. The lens spectroscopic analysis system will become larger.

In order to correct the loss of the optical multiplexer 108, it is necessary to detect the amount of light after the excitation light and the detection light are combined, but the excitation light and the detection light combined in the optical fiber. It is difficult to detect separately.

An object of the present invention is to provide a small thermal lens spectroscopic analysis system and a thermal lens signal correction method capable of accurately measuring a sample even if the thermal lens signal intensity changes due to a change in the external environment. It is in. F

Disclosure of the invention

In order to achieve the above object, according to a first aspect of the present invention, a chip having a groove into which a liquid sample is injected and excitation excited from a light source to the liquid sample via an optical transmission path are provided. In a thermal lens spectroscopic analysis system including an objective lens that collects light and detection light to generate a thermal lens signal, a light amount measurement unit that measures the light amount of the excitation light and the detection light, and the thermal lens signal A thermal lens signal intensity measuring unit for measuring the intensity; a first ratio between the predetermined light amount of the excitation light and the measured light amount of the excitation light; and the predetermined light amount of the detection light and the measured detection light A ratio calculation unit for calculating a second ratio with the light amount; and the measured thermal lens signal intensity, the first ratio, and the Z or the second ratio. Thermal lens signal to correct the intensity of the thermal lens signal Intensity detecting correcting part and Bei Ru thermal lens spectrometry system is provided.

In the first aspect of the present invention, it is preferable that the light quantity measuring unit for measuring the light quantity of the d excitation light and the detection light comprises one detector force.

In the first aspect of the present invention, the light amount measuring unit for measuring the light amount of the eye U excitation light and the detection light, and the thermal lens signal intensity measuring unit for measuring the intensity of the thermal lens signal are one detector. It is preferable to consist of

In the first aspect of the present invention, the detector includes a light transmission filter in which an excitation light transmission filter that transmits only BU sti excitation light and a detection light transmission filter that transmits only the detection light are interchangeable, and the detector It is preferable to measure the amount of excitation light and detection light transmitted through the light transmission filter.

In the first aspect of the present invention, it is preferable to correct the intensity of the thermal lens signal based on the output value of the detector.

In the first aspect of the present invention, the output value of the detector is a current value. Is preferred.

In the first aspect of the present invention, the output value of the detector is preferably a voltage value.

In the first aspect of the present invention, it is preferable that an audio input terminal for measuring the voltage value is provided.

In the first aspect of the present invention, the optical transmission path is preferably one optical fiber.

In the first aspect of the present invention, the objective lens is preferably a rod lens.

In the first aspect of the present invention, the thermal lens signal is preferably obtained by high-speed Fourier transform processing.

In order to achieve the above object, according to the second aspect of the present invention, the thermal lens signal generated by irradiating the liquid sample injected into the groove in the chip with excitation light and detection light is generated. In the thermal lens signal correction method for correcting, a light quantity measurement step for measuring the light quantity of the excitation light and the detection light, a thermal lens signal intensity measurement step for measuring the intensity of the thermal lens signal, and a predetermined light quantity of the excitation light And a ratio calculating step for calculating a first ratio between the measured light amount of the excitation light and a second ratio between the predetermined light amount of the detected light and the measured light amount of the detected light; and A thermal lens signal intensity detection correction step for correcting the intensity of the measured thermal lens signal by integrating the intensity of the thermal lens signal, the first ratio, and / or the second ratio. Heat lens Correction method is provided. Brief Description of Drawings

FIG. 1 is a diagram schematically showing a configuration of a thermal lens spectroscopic analysis system according to an embodiment of the present invention. FIG. 2 is a diagram schematically showing the configuration of the detection apparatus in FIG.

3A, B, and C are diagrams showing the relationship between the change in the light intensity of the excitation light and detection light and the change in the thermal lens signal intensity in the thermal lens spectroscopic analysis system of FIG. 1, and FIG. 3A shows only the light intensity of the excitation light. FIG. 3B is a diagram showing the relationship between the excitation light measurement intensity and the thermal lens signal intensity when only the light intensity of the detection light changes. FIG. 3C is a diagram showing the relationship between the integrated value of the measured intensity of the excitation light and the detection light and the thermal lens signal intensity when the light intensity of the excitation light and the light intensity of the detection light change simultaneously. .

Fig. 4 is a diagram schematically showing the configuration of a conventional thermal lens spectroscopic analysis system. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.

FIG. 1 is a diagram schematically showing a configuration of a thermal lens spectroscopic analysis system according to an embodiment of the present invention.

In FIG. 1, the thermal lens spectroscopic analysis system 10 includes a microchemical chip 2 having a groove 1 into which a sample in liquid is poured, and a microchemical chip 2 above the groove 1 on the micro-mouth chemical chip 2. A refractive index distribution type sensor such as a cylindrical self-lock (registered trademark) that is arranged at intervals and collects light propagating from an optical fiber 5 (described later) in a groove 1 to generate a thermal lens signal. A single-mode optical fiber 5 for visible light that is disposed above the refractive index distribution type lens 3 and propagates light to the refractive index distribution type lens 3; The ferrule 4 holding the eye bar 5, the sleeve 6 that fixes the gradient index rod lens 3 and the optical fiber 5 through the ferrule 4, and the optical fiber 1 5 are connected to the optical fiber 1. 1 through 5 A light source unit 7 irradiates the sample in liquid in the groove 1 of the chemical chip 2 with excitation light and irradiates detection light to the thermal lens generated in the sample in liquid by the irradiated excitation light, and a microphone. The detection light is detected via the thermal lens generated in the sample in the liquid in the groove 1 of the microchemical chip 2 by the excitation light emitted from the light source unit 7 and placed under the chemical chip 2. And a detecting device 8 for performing the above operation.

The microchemical chip 2 has a groove 1 through which the sample in the liquid flows during operations such as mixing, stirring, synthesis, separation, extraction, and detection of the sample in the liquid by the thermal lens spectroscopic analysis system 10. is doing.

The material of the microchemical chip 2 is desirably glass from the viewpoint of durability and chemical resistance, and, in view of its use for biological samples such as cells, for example, DNA analysis, acid resistance, Glass having high alkali resistance, specifically, borosilicate glass, soda lime glass, aluminoborosilicate glass, and quartz glass are preferred. However, organic substances such as plastic can be used by limiting the application.

The light source unit 7 is connected to the excitation light source 14 that outputs the excitation light, and the excitation light source 14 that is connected to the excitation light source 14, for example, emits excitation light having a wavelength of 6 58 nm, for example. Modulator 15 that modulates to On and Off with a period of 1 kHz, for example, a detection light source 16 that outputs detection light with a wavelength of 785 nm, a pump light source 14 and The excitation light source 1 and the detection light source 1 output from the excitation light source 1 4 connected to the detection light source 1 6 via optical fibers — 1 7 and 1 8, respectively, and connected to the optical fiber 1 5. 6 includes a multiplexer 19 that multiplexes the detection light output from 6 and multiplexes the detection light and the detection light into the optical fiber 5.

In the light source unit 7, pump light and detection light source output from the pump light source 14 using a dichroic mirror instead of the multiplexer 19 The detection light output from 16 may be combined, and the excitation light and detection light combined in the optical fiber may be incident.

The detection device 8 is a position facing a transmission member 20 having a pinhole 20 a through which only a part of light is transmitted and a groove 1 of the microchemical chip 2 through a predetermined interval. A filter 2 1 disposed at a position opposite to the optical fiber 1 and separating and selectively transmitting the combined excitation light and detection light; A photoelectric converter (silicon diode) for detecting the amount of excitation light and detection light and the intensity of the thermal lens signal 2 2 (detector) ) And a personal computer (PC) 2 5 (thermal lens signal intensity detection correction unit) connected to the photoelectric converter 2 2 via an IV amplifier (current-voltage conversion amplifier) 2 3 and a voltmeter 2 4 Consists of. The voltmeter 2 4 can be inserted between the I V amplifier 2 3 and P C 2 5, or it can be connected to the I V amplifier 2 3 independently. In this case, the I V amplifier 2 3 and PC 25 are directly connected. The signal obtained from the photoelectric converter 22 is then analyzed at P C 25. FIG. 2 is a diagram schematically showing the configuration of the detection apparatus in FIG.

In Fig. 2, the filter 2 1 has a band width of 20 nm that transmits only excitation light with a wavelength of 6 58 nm, a diameter of 1 2.5 mm (^ and a band pass filter for excitation light of 3.0 and a wavelength of 7 8 A bandpass filter 3 1 for detecting light with a band width of 20 nm that transmits only 5 nm of detection light and a diameter of 12.5 πιιηφ. 3 1, 3 1 Can be replaced by a left-right replacement method, a rotation method, a sampling method, or the like.

The measurement of the thermal lens signal intensity was performed in a state where the detection light bandpass filter 31 was placed at a position facing the pinhole 20 a formed in the transmission member 20 (FIG. 2). This is done by inputting the output signal of the IV amplifier 23 to the PC 25 via the voltmeter 24. Also to PC 2 5 The output signal is input via a DA converter (not shown) or an audio input terminal (not shown). The output signal input to PC 25 is subjected to high-speed Fourier transform (FFT) processing and detected as a thermal lens signal. Hereinafter, the relationship between the change in the light amount of the excitation light and the detection light and the change in the intensity of the thermal lens signal in the thermal lens spectroscopic analysis system 10 will be described.

In thermal lens spectroscopy, the amount of light absorbed by the detection target substance is measured via the thermal lens phenomenon, so the amount (concentration) of the substance cannot be calculated directly from the thermal lens signal alone. Therefore, by measuring the thermal lens signals of various sample solutions containing the detection target substance at various known concentrations, and using a calibration curve drawn based on the measured thermal lens signals, it is contained in the sample solution of unknown concentration. The concentration of the detection target substance will be calculated. In this case, since the concentration is determined by the ratio with the measurement result when the calibration curve is drawn, it is important to measure the thermal lens signal of the sample solution of unknown concentration under the same conditions as when the calibration curve is drawn. Become.

However, the amount of light output from the excitation light source and the detection light source changes due to the influence of changes in the external environment such as a temperature change, or the loss of the optical multiplexer 108 (in each of the excitation light and the detection light). The ratio of the amount of light that is input to the multiplexer and the amount of light that is output) or the relative position of the lens 1002, the plate member 120, and the photoelectric converter 4001 The fluctuation of the thermal lens signal strength due to the. When such fluctuations occur and a sample solution with an unknown concentration is measured under conditions different from those when the calibration curve is measured, the thermal lens signal intensity includes changes due to external fluctuation factors. It is not possible to obtain the correct concentration. Therefore, it is necessary to correct the thermal lens signal intensity changed due to the influence of external environmental changes to the value when measured under the conditions of the calibration curve.

Fig. 3 A, B, and C 'are excitations in the thermal lens spectroscopic system 10 of Fig. 1. FIG. 3A is a diagram showing the relationship between the change in the light intensity of the electromotive light and the detection light and the change in the thermal lens signal intensity. FIG. 3A shows the relationship between the excitation light measurement intensity and the thermal lens signal intensity when only the excitation light intensity changes. FIG. 3B is a diagram showing the relationship between the detected light measurement intensity and the thermal lens signal intensity when only the detection light quantity changes, and FIG. 3C is the excitation light quantity and detection light. FIG. 6 is a diagram showing the relationship between the integrated value of the measured intensity of excitation light and detection light and the thermal lens signal intensity when the amount of light changes simultaneously.

In Fig. 3A, the vertical axis shows the thermal lens signal intensity (mV), the horizontal axis shows the output of the IV amplifier 23 (the first IV output) (V), which represents the measured light intensity of the excitation light, and the thermal lens signal The intensity (mV) is proportional to the first IV output (V). In Fig. 3B, the vertical axis shows the thermal lens signal intensity (mV), and the horizontal axis shows the output of the IV amplifier 23 (second IV output) (V), which represents the measured light intensity of the detected light. The thermal lens signal strength (mV) is proportional to the second IV output (V). That is, when one of the excitation light and the detection light changes, the thermal lens signal intensity (mV) is proportional to the first or second IV output (V). Here, a method of correcting the thermal lens signal intensity when the amount of excitation light or detection light changes will be described. For example, the measurement value of the excitation light used when drawing the calibration curve is 2.4 V, the measurement value of the excitation light when measuring a sample solution of unknown concentration is 2.2 V, and heat Assume that the lens signal strength is 3. lm V. In this case, if the calibration curve is compared with the thermal lens signal intensity as it is, the concentration obtained will be as low as the amount of excitation light decreases. Therefore, the thermal lens signal intensity of the unknown sample solution is corrected to 3.4 mV in consideration of the change in the amount of excitation light. Here, 3.4 mV is the first ratio (measured light intensity of excitation light / measured light intensity of excitation light) to the measured value of thermal lens signal intensity in a sample solution of unknown concentration. That is, the value calculated from 3. IX (2.4 / 2.2). The thermal lens signal intensity is corrected in the same way for changes in the amount of detection light.

In Fig. 3C, the vertical axis shows the thermal lens signal intensity (mV), and the horizontal axis shows the integrated value of the first IV output (V) and the second IV output (V). It is proportional to the lens signal strength (mV). That is, when the amount of excitation light and detection light changes, the thermal lens signal intensity (mV) is proportional to the integrated value of the first I V output (V) and the second I V output (V).

In general, it is known that the thermal lens signal intensity is proportional to the excitation light intensity or detection light intensity incident on the sample. However, when the light amounts of the excitation light and the detection light are changed at the same time, it has not been understood what relationship exists between the respective light amounts and the thermal lens signal intensity. As a result of repeated studies, as shown in Fig. 3C, it was found that the value obtained by multiplying the measured light intensity of the excitation light and the measured light intensity of the detection light is proportional to the thermal lens signal intensity.

Here, a method for correcting the thermal lens signal intensity when the light amounts of the excitation light and the detection light are simultaneously changed will be described. For example, the excitation light used when measuring a sample solution with an unknown concentration is 2.5 V, the detection light measurement value is 5.4 V, and the detection light is 5.4 V. Suppose that the measured light intensity is 2.2 V, the detected light intensity is 4.8 V, and the thermal lens signal intensity is 2.75 mV. In this case, if the calibration curve is compared with the thermal lens signal intensity as it is, the resulting concentration will be lower due to the decrease in the amount of excitation light and detection light. Therefore, the intensity of the thermal lens of the sample solution of unknown concentration is corrected to 3.5 mV in consideration of the amount of change in the excitation light and detection light. Here, 3.5 mV is the first ratio (measured light intensity of the excitation light) and the ratio of the first to the measured value of the thermal lens signal intensity in the sample solution of unknown concentration and The value multiplied by the integrated value of the second ratio (predetermined light intensity measurement value of the detection light and measurement light intensity measurement value of the detection light), that is, 2.75 x (2.5 no 2.2) x (5.4 / 4.8) This is a calculated value.

In the above explanation, the amount of excitation light and detection light when measuring a sample of unknown concentration (measurement light amount) The case where the light amount when measuring the force calibration curve (predetermined light amount) is lower However, if the measured light intensity is higher than the predetermined light intensity, it is calculated in the same way using the same formula.

According to the present embodiment, the measured value of the thermal lens signal intensity, the first ratio (predetermined light amount of excitation light Z, measured light amount of excitation light), and the second ratio (predetermined light amount of detection light, measurement of detection light) Since the measured value of the thermal lens signal intensity is corrected by integrating the (light quantity), the sample can be accurately measured even if the thermal lens signal intensity changes due to changes in the external environment.

According to the present embodiment, the light quantity measurement of the excitation light and the detection light and the measurement of the thermal lens signal are performed by one photoelectric converter 2 2 (detector). Only one detector is required, and the thermal lens spectroscopic analysis system 10 can be simplified and miniaturized. In addition, since changes in the amount of excitation light and detection light are measured after the excitation light and detection light have passed through the sample, there is no need to place a measurement optical system in the middle of the optical fiber that is the optical transmission path, and heat The lens spectroscopic analysis system 10 can be simplified and miniaturized, and the change in the light amount of the excitation light and detection light can be accurately measured.

According to the present embodiment, the light quantity measurement of the excitation light and the detection light is performed by using the bandpass filters 30 and 31 that allow only one of the lights to pass through. Correction can be made only by adding a mechanism that replaces the two filters to the detection device 8 used in the 10 so that the thermal lens spectroscopic analysis system 10 can be simplified and downsized. The

In this embodiment, only one of the excitation light and the detection light is transmitted. The band-pass fino-letters 3 0 and 3 1 were used as the filter to be used. Moyore.

In the present embodiment, the gradient index rod lens 3 is arranged above the groove 1 on the microchemical chip 2 with a predetermined interval, but the present invention is not limited to this. It may be placed on the microchemical chip 2 or may be adhered on the microchemical chip 2.

In this embodiment, the output voltage value of the IV amplifier 23 is read by the voltmeter 24. However, the present invention is not limited to this, and the voltage is measured by measuring the input value to the audio input terminal. The value may be measured. This eliminates the need to install the voltmeter 2 4 between the I V amplifier 2 3 and the PC 2 5.

In the present embodiment, the transmissive member 20 is provided. However, the present invention is not limited to this, and the transmissive member 20 may not be provided. Industrial applicability

According to the thermal lens spectroscopic analysis system of the first aspect of the present invention and the thermal lens signal correction method of the second aspect of the present invention, the intensity of the measured thermal lens signal, the predetermined amount of excitation light, and the measured excitation Correct the intensity of the measured thermal lens signal by integrating the first ratio with the light intensity and the second ratio between the predetermined detection light intensity and the measured detection light intensity. Therefore, the sample can be accurately measured even if the heat lens signal intensity changes due to external environmental changes.

According to the thermal lens spectroscopic analysis system of the first aspect of the present invention, the amount of excitation light and the amount of detection light used to correct the intensity of the measured thermal lens signal are measured by a single detector. The thermal lens spectroscopic analysis system can be miniaturized. According to the thermal lens spectroscopic analysis system of the first aspect of the present invention, the intensity of the thermal lens signal is measured by one detector in addition to the light amount of the excitation light and the light amount of the detection light. The structure can be made simpler and smaller.

According to the thermal lens spectroscopic analysis system of the first aspect of the present invention, the mechanism that measures the light amount of the excitation light and the light amount of the detection light by replacing the filter that transmits only the excitation light and the filter that transmits only the detection light. As a result, the structure of the detector can be simplified and the thermal lens spectroscopic analysis system can be further miniaturized, and the change in the amount of excitation light and detection light can be reliably measured to reliably correct the thermal lens signal. can do.

Claims

The scope of the claims

1. a chip having a groove in which a liquid sample is injected, and an objective lens that collects excitation light and detection light propagated from a light source through an optical transmission path to the liquid sample to generate a thermal lens signal; A thermal lens spectroscopic analysis system comprising: a light quantity measuring unit that measures the light quantity of the excitation light and the detection light; a thermal lens signal intensity measuring part that measures the intensity of the thermal lens signal; and a predetermined light quantity of the excitation light. A ratio calculating unit that calculates a first ratio between the measured light amount of the excitation light and a second ratio between the predetermined light amount of the detected light and the measured light amount of the detected light; A thermal lens signal intensity detection correction unit that corrects the intensity of the measured thermal lens signal by integrating the intensity of the thermal lens signal, the first ratio, and / or the second ratio. Thermal lens component characterized by comprising Analysis system.

2. The thermal lens spectroscopic analysis system according to claim 1, wherein the light quantity measuring unit for measuring the quantity of the excitation light and the detection light comprises a single detector.

3. The light quantity measurement unit that measures the light quantity of the excitation light and the detection light and the thermal lens signal intensity measurement unit that measures the intensity of the thermal lens signal are composed of one detector. The thermal lens spectroscopic analysis system according to paragraph 1 of the above.

4. An excitation light transmission filter that transmits only the excitation light and a detection light transmission filter that transmits only the detection light are arranged to be interchangeable, and the detector includes the light transmission filter. The thermal lens spectroscopic analysis system according to claim 2, characterized in that the amount of excitation light and detection light transmitted through one is measured.

5. Correct the intensity of the thermal lens signal based on the output value of the detector The thermal lens spectroscopic analysis system according to claim 2, characterized in that this is a feature.

6. The thermal lens spectroscopic analysis system according to claim 5, wherein the output value of the detector is a current value.

7. The thermal lens spectroscopic analysis system according to claim 5, wherein the output value of the detector is a voltage value.

8. The thermal lens spectroscopic analysis system according to claim 7, further comprising an audio input terminal for measuring the voltage value.

9. The thermal lens spectroscopic analysis system according to claim 1, wherein the optical transmission path is a single optical fiber.

10. The thermal lens spectroscopic analysis system according to claim 1, wherein the objective lens is a rod lens.

11. The thermal lens spectroscopic analysis system according to claim 1, wherein the thermal lens signal is obtained by a high-speed Fourier transform process.

1 2. In a thermal lens signal correction method for correcting a thermal lens signal generated by irradiating a liquid sample injected into a groove in a chip with excitation light and detection light, the excitation light and the detection light A light amount measuring step for measuring the light amount of the thermal lens, a thermal lens signal intensity measuring step for measuring the intensity of the thermal lens signal, a first ratio between the predetermined light amount of the excitation light and the measured light amount of the excitation light, and A ratio calculating step of calculating a second ratio between the predetermined light quantity of the pruning detection light and the measured light quantity of the detected light; the intensity of the measured thermal lens signal; the first ratio; and Z or the A thermal lens signal correction method comprising: a thermal lens signal intensity detection correction step of correcting the intensity of the measured thermal lens signal by integrating the second ratio.