WO2006101983A3 - Chemically inducible expression of biosynthetic pathways - Google Patents

Chemically inducible expression of biosynthetic pathways Download PDF

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Publication number
WO2006101983A3
WO2006101983A3 PCT/US2006/009531 US2006009531W WO2006101983A3 WO 2006101983 A3 WO2006101983 A3 WO 2006101983A3 US 2006009531 W US2006009531 W US 2006009531W WO 2006101983 A3 WO2006101983 A3 WO 2006101983A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
constructs
enzymes
transgenes
transgene
Prior art date
Application number
PCT/US2006/009531
Other languages
French (fr)
Other versions
WO2006101983A2 (en
Inventor
Lauralynn Kourtz
Oliver P Peoples
Kristi D Snell
Original Assignee
Metabolix Inc
Lauralynn Kourtz
Oliver P Peoples
Kristi D Snell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolix Inc, Lauralynn Kourtz, Oliver P Peoples, Kristi D Snell filed Critical Metabolix Inc
Priority to BRPI0608637-3A priority Critical patent/BRPI0608637A2/en
Priority to EP06738572A priority patent/EP1863916B1/en
Priority to JP2008502042A priority patent/JP2008532553A/en
Priority to CA2600882A priority patent/CA2600882C/en
Priority to AU2006227634A priority patent/AU2006227634B2/en
Priority to AT06738572T priority patent/ATE521710T1/en
Publication of WO2006101983A2 publication Critical patent/WO2006101983A2/en
Publication of WO2006101983A3 publication Critical patent/WO2006101983A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Abstract

Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent.
PCT/US2006/009531 2005-03-16 2006-03-16 Chemically inducible expression of biosynthetic pathways WO2006101983A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
BRPI0608637-3A BRPI0608637A2 (en) 2005-03-16 2006-03-16 recombinant vector for enzyme expression, transformed plant cell, method for biosynthetic product production
EP06738572A EP1863916B1 (en) 2005-03-16 2006-03-16 Chemically inducible expression of biosynthetic pathways
JP2008502042A JP2008532553A (en) 2005-03-16 2006-03-16 Chemically inducible expression of biosynthetic pathways
CA2600882A CA2600882C (en) 2005-03-16 2006-03-16 Chemically inducible expression of biosynthetic pathways
AU2006227634A AU2006227634B2 (en) 2005-03-16 2006-03-16 Chemically inducible expression of biosynthetic pathways
AT06738572T ATE521710T1 (en) 2005-03-16 2006-03-16 CHEMICALLY INDUCABLE EXPRESSION OF BIOSYNTHESIS PATHWAYS

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US66223505P 2005-03-16 2005-03-16
US60/662,235 2005-03-16
US66976605P 2005-04-08 2005-04-08
US60/669,766 2005-04-08

Publications (2)

Publication Number Publication Date
WO2006101983A2 WO2006101983A2 (en) 2006-09-28
WO2006101983A3 true WO2006101983A3 (en) 2007-02-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/009531 WO2006101983A2 (en) 2005-03-16 2006-03-16 Chemically inducible expression of biosynthetic pathways

Country Status (8)

Country Link
US (3) US7732680B2 (en)
EP (1) EP1863916B1 (en)
JP (1) JP2008532553A (en)
AT (1) ATE521710T1 (en)
AU (1) AU2006227634B2 (en)
BR (1) BRPI0608637A2 (en)
CA (2) CA2600882C (en)
WO (1) WO2006101983A2 (en)

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US8487159B2 (en) * 2008-04-28 2013-07-16 Metabolix, Inc. Production of polyhydroxybutyrate in switchgrass
AU2009285590B2 (en) * 2008-08-29 2015-02-05 E. I. Du Pont De Nemours And Company Manipulation of SNF1 protein kinase activity for altered oil content in oleaginous organisms
US20100055671A1 (en) * 2008-09-03 2010-03-04 National Tsing Hua University Method for Determining Transduction Efficiency and Virus Dosage of Baculovirus
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CA2773703A1 (en) 2009-09-15 2011-03-24 Metabolix, Inc. Generation of high polyhydroxybutrate producing oilseeds
KR101719776B1 (en) 2014-11-13 2017-04-10 대한민국 A promoter for high-expression of gene involved in fatty acid metabolisms and its uses
US10533192B2 (en) 2015-12-22 2020-01-14 Nutech Ventures Production of isoprene by methane-producing archaea
CN116875524B (en) * 2023-09-08 2024-03-26 清华大学 Method for regulating and controlling polygene expression in microorganism and application thereof

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Also Published As

Publication number Publication date
WO2006101983A2 (en) 2006-09-28
AU2006227634B2 (en) 2010-07-08
CA2600882A1 (en) 2006-09-28
US8742203B2 (en) 2014-06-03
US20100196974A1 (en) 2010-08-05
EP1863916B1 (en) 2011-08-24
ATE521710T1 (en) 2011-09-15
CA2800359A1 (en) 2006-09-28
US20070067869A1 (en) 2007-03-22
JP2008532553A (en) 2008-08-21
CA2600882C (en) 2013-02-26
EP1863916A2 (en) 2007-12-12
US20130042371A1 (en) 2013-02-14
BRPI0608637A2 (en) 2010-01-19
AU2006227634A1 (en) 2006-09-28
US7732680B2 (en) 2010-06-08

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