WO2006074194A3 - Engineered phosphite dehydrogenase mutants for nicotinamide cofactor regeneration - Google Patents

Engineered phosphite dehydrogenase mutants for nicotinamide cofactor regeneration Download PDF

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Publication number
WO2006074194A3
WO2006074194A3 PCT/US2006/000135 US2006000135W WO2006074194A3 WO 2006074194 A3 WO2006074194 A3 WO 2006074194A3 US 2006000135 W US2006000135 W US 2006000135W WO 2006074194 A3 WO2006074194 A3 WO 2006074194A3
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WO
WIPO (PCT)
Prior art keywords
cofactor regeneration
phosphite dehydrogenase
nicotinamide cofactor
dehydrogenase mutants
engineered phosphite
Prior art date
Application number
PCT/US2006/000135
Other languages
French (fr)
Other versions
WO2006074194A2 (en
Inventor
Huimin Zhao
Ryan Woodyer
William Metcalf
Der Donk Wilfred A Van
Tyler Johannes
Original Assignee
Biotechnology Res & Dev
Univ Illinois
Huimin Zhao
Ryan Woodyer
William Metcalf
Der Donk Wilfred A Van
Tyler Johannes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotechnology Res & Dev, Univ Illinois, Huimin Zhao, Ryan Woodyer, William Metcalf, Der Donk Wilfred A Van, Tyler Johannes filed Critical Biotechnology Res & Dev
Publication of WO2006074194A2 publication Critical patent/WO2006074194A2/en
Publication of WO2006074194A3 publication Critical patent/WO2006074194A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/36—Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Phosphite dehydrogenase mutant enzymes provide relaxed cofactor specificity, increased thermostability, increased activity, solubility, and expression over the wild-type enzyme. The mutant enzymes are useful for nicotinamide cofactor regeneration.
PCT/US2006/000135 2005-01-05 2006-01-03 Engineered phosphite dehydrogenase mutants for nicotinamide cofactor regeneration WO2006074194A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64157905P 2005-01-05 2005-01-05
US60/641,579 2005-01-05

Publications (2)

Publication Number Publication Date
WO2006074194A2 WO2006074194A2 (en) 2006-07-13
WO2006074194A3 true WO2006074194A3 (en) 2006-10-19

Family

ID=36589316

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/000135 WO2006074194A2 (en) 2005-01-05 2006-01-03 Engineered phosphite dehydrogenase mutants for nicotinamide cofactor regeneration

Country Status (1)

Country Link
WO (1) WO2006074194A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7045725B2 (en) 2017-04-27 2022-04-01 コデクシス， インコーポレイテッド Ketoreductase polypeptides and polynucleotides

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5892621B2 (en) * 2011-04-26 2016-03-23 国立大学法人広島大学 Method for producing phosphite dehydrogenase protein and use thereof
US9499822B2 (en) 2012-08-09 2016-11-22 Hiroshima University Method for selectively culturing microorganism using phosphite dehydrogenase gene as marker
EP4092126A1 (en) 2021-03-29 2022-11-23 Instytut Katalizy I Fizykochemii Powierzchni Im.Jerzego Habera Polskiej Akademii Nauk A method of enzymatic reduction of the oxidized nicotinamide adenine dinucleotide and carboxylic acids
CN114634955B (en) * 2022-04-22 2023-07-18 金达威生物技术(江苏)有限公司 Method for preparing L-tertiary leucine by biological enzyme catalysis and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004108912A2 (en) * 2003-06-11 2004-12-16 Biotechnology Research And Development Corporation Phosphite dehydrogenase mutants for nicotinamide cofactor regeneration

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004108912A2 (en) * 2003-06-11 2004-12-16 Biotechnology Research And Development Corporation Phosphite dehydrogenase mutants for nicotinamide cofactor regeneration

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
COSTAS A M G ET AL: "Purification and Characterization of a Novel Phosphorus-oxidizing Enzyme from Pseudomonas stutzeri WM88", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 276, no. 20, 22 February 2001 (2001-02-22), pages 17429 - 17436, XP002979983, ISSN: 0021-9258 *
VRTIS J M ET AL: "Phosphite dehydrogenase: a versatile cofactor-regeneration enzyme", ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, WILEY VCH VERLAG, WEINHEIM, DE, vol. 41, no. 17, 2 September 2002 (2002-09-02), pages 3257 - 3259, XP002311088, ISSN: 1433-7851 *
WOODYER R ET AL: "Relaxing the nicotinamide cofactor specificity of phosphite dehydrogenase by rational design", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 42, no. 40, 14 October 2003 (2003-10-14), pages 11604 - 11614, XP002311089, ISSN: 0006-2960 *
ZHAO HUIMIN ET AL: "Directed evolution of enzymes and pathways for industrial biocatalysis", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 13, no. 2, April 2002 (2002-04-01), pages 104 - 110, XP002311087, ISSN: 0958-1669 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7045725B2 (en) 2017-04-27 2022-04-01 コデクシス， インコーポレイテッド Ketoreductase polypeptides and polynucleotides

Also Published As

Publication number Publication date
WO2006074194A2 (en) 2006-07-13

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