WO2006073472A3 - Kits and processes for removing contaminants from nucleic acids in environmental and biological samples - Google Patents

Kits and processes for removing contaminants from nucleic acids in environmental and biological samples Download PDF

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Publication number
WO2006073472A3
WO2006073472A3 PCT/US2005/017933 US2005017933W WO2006073472A3 WO 2006073472 A3 WO2006073472 A3 WO 2006073472A3 US 2005017933 W US2005017933 W US 2005017933W WO 2006073472 A3 WO2006073472 A3 WO 2006073472A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
environmental
compositions
biological samples
kits
Prior art date
Application number
PCT/US2005/017933
Other languages
French (fr)
Other versions
WO2006073472A2 (en
Inventor
Mark N Brolaski
Raveendran J Venugopal
David Stolow
Original Assignee
Mo Bio Lab Inc
Mark N Brolaski
Raveendran J Venugopal
David Stolow
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mo Bio Lab Inc, Mark N Brolaski, Raveendran J Venugopal, David Stolow filed Critical Mo Bio Lab Inc
Priority to CA2567599A priority Critical patent/CA2567599C/en
Priority to EP05856744.7A priority patent/EP1756136B1/en
Priority to AU2005323451A priority patent/AU2005323451B2/en
Priority to JP2007527515A priority patent/JP5112064B2/en
Priority to DK05856744.7T priority patent/DK1756136T3/en
Priority to ES05856744.7T priority patent/ES2529044T3/en
Publication of WO2006073472A2 publication Critical patent/WO2006073472A2/en
Publication of WO2006073472A3 publication Critical patent/WO2006073472A3/en
Priority to HK07109399.1A priority patent/HK1102229A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Abstract

The invention provides methods and compositions, e.g., kits, for removing contaminants from nucleic acids in a sample, e.g., environmental or biological samples such as soil, food, plant, animal, microorganism or water samples. The invention provides methods and compositions for isolating nucleic acids from environmental and biological samples in a scaleable process free of contaminating substances that inhibit PCR and other downstream applications. Exemplary sample types include soil, water, plant and food. The methods and compositions of the invention can be used for isolating and/or detecting nucleic acids from prokaryotic and eukaryotic organisms and for detecting multiple types of organisms in a sample. Thus, the methods and compositions of the invention are useful for detecting organisms pertaining to agriculture, forensics biology and/or combating bioterrorism.
PCT/US2005/017933 2004-05-21 2005-05-20 Kits and processes for removing contaminants from nucleic acids in environmental and biological samples WO2006073472A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA2567599A CA2567599C (en) 2004-05-21 2005-05-20 Kits and processes for removing contaminants from nucleic acids in environmental and biological samples
EP05856744.7A EP1756136B1 (en) 2004-05-21 2005-05-20 Kits and processes for removing contaminants from nucleic acids in environmental and biological samples
AU2005323451A AU2005323451B2 (en) 2004-05-21 2005-05-20 Kits and processes for removing contaminants from nucleic acids in environmental and biological samples
JP2007527515A JP5112064B2 (en) 2004-05-21 2005-05-20 Kits and methods for removing contaminants from nucleic acids in environmental and biological samples
DK05856744.7T DK1756136T3 (en) 2004-05-21 2005-05-20 KIT AND METHODS FOR REMOVING IMPURITIES FROM nucleic acids in ENVIRONMENTAL AND BIOLOGICAL SAMPLES
ES05856744.7T ES2529044T3 (en) 2004-05-21 2005-05-20 Kits and procedures to remove nucleic acid contaminants in environmental and biological samples
HK07109399.1A HK1102229A1 (en) 2004-05-21 2007-08-28 Kits and processes for removing contaminants from nucleic acids in environmental and biological samples

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US57335804P 2004-05-21 2004-05-21
US60/573,358 2004-05-21
US57417904P 2004-05-24 2004-05-24
US60/574,179 2004-05-24

Publications (2)

Publication Number Publication Date
WO2006073472A2 WO2006073472A2 (en) 2006-07-13
WO2006073472A3 true WO2006073472A3 (en) 2006-09-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/017933 WO2006073472A2 (en) 2004-05-21 2005-05-20 Kits and processes for removing contaminants from nucleic acids in environmental and biological samples

Country Status (9)

Country Link
US (2) US7459548B2 (en)
EP (1) EP1756136B1 (en)
JP (1) JP5112064B2 (en)
AU (1) AU2005323451B2 (en)
CA (1) CA2567599C (en)
DK (1) DK1756136T3 (en)
ES (1) ES2529044T3 (en)
HK (1) HK1102229A1 (en)
WO (1) WO2006073472A2 (en)

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Also Published As

Publication number Publication date
ES2529044T3 (en) 2015-02-16
HK1102229A1 (en) 2007-11-09
JP2008500066A (en) 2008-01-10
DK1756136T3 (en) 2015-01-19
US20090111159A1 (en) 2009-04-30
CA2567599C (en) 2015-07-21
AU2005323451A1 (en) 2006-07-13
WO2006073472A2 (en) 2006-07-13
EP1756136A4 (en) 2010-10-06
US7459548B2 (en) 2008-12-02
CA2567599A1 (en) 2006-07-13
EP1756136A2 (en) 2007-02-28
JP5112064B2 (en) 2013-01-09
US20050282202A1 (en) 2005-12-22
AU2005323451B2 (en) 2010-11-18
EP1756136B1 (en) 2014-10-29

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