WO2006064870A1

WO2006064870A1 - Fluid fat composition containing data-providing nucleic acid

Info

Publication number: WO2006064870A1
Application number: PCT/JP2005/023038
Authority: WO
Inventors: Hiroshi Yokoyama; Masahiko Yamanaka; Kentarou Watanabe; Tsunehiko Higuchi; Shigeki Hirabayashi
Original assignee: Nissan Motor Co., Ltd.
Priority date: 2004-12-15
Filing date: 2005-12-15
Publication date: 2006-06-22
Also published as: JP2006169339A

Abstract

It is intended to provide a fluid fat composition containing a data-providing nucleic acid wherein the data-providing nucleic acid contained therein enables the individual and specific determination of the origin and history of the product. This fluid fat composition containing a data-providing nucleic acid contains the data-providing nucleic acid comprising a site which has an arbitrary and known base sequence. The above-described fluid fat composition containing a data-providing nucleic acid contains the data-providing nucleic acid comprising a site which has an arbitrary and known base sequence and the data-providing nucleic acid is carried on fine particles. Furthermore, the above-described fluid fat composition containing a data-providing nucleic acid contains the data-providing nucleic acid comprising a site which has an arbitrary and known base sequence and the content of the data-providing nucleic acid ranges from 0.5 to 500 μg per 100 g of the composition.

Description

Specification

Fluidized oil composition containing information nucleic acid

Technical field

[0001] The present invention relates to an information nucleic acid-containing fluid fat composition, and more particularly to a fluid oil composition containing an information nucleic acid that can be used for individual authentication.

Background art

Conventionally, for individual authentication, watermark printing of license plates, banknotes, etc., photographs of IC chips and credit cards have been used.

However, these individual authentication means have a drawback that they can be removed from the product by peeling, cutting, erasing and the like. For this reason, development of authentication information that cannot be removed from the product, that is, authentication information that does not disappear is expected.

[0003] On the other hand, DNA is an information biomolecule originally possessed by all living organisms and including all genetic information in each organism. Many of them correspond to the amino acid sequences of many proteins. In other words, deoxyadenosine (dA), deoxyguanosine (dG), deoxycytosine (dC) and deoxythymine (dT) forces are bound with a certain direction through S-phosphate bond, and the number of bases If n is ^n, there are 4 ⁿ types of DNA of that length. Thus, for example, there can be about 4.3 billion distinct DNAs with only 16 bases. At present, any DNA sequence with several tens of base sequences can be synthesized arbitrarily. In addition, if the amount of DNA exceeds a certain level, the sequence can be automatically determined by an automatic sequence reader (sequencer).

[0004] Against this background, by using a forgery-proof label that contains DNA in a water-insoluble medium for the product, the presence or absence of the DNA can be used as a clue to clarify the authenticity of the product. Has been proposed (see Patent Document 1).

Patent Document 1: Japanese Unexamined Patent Application Publication No. 2004-159502

Disclosure of the invention

Problems to be solved by the invention [0005] However, the technique described in Patent Document 1 basically relates to a method of mixing DNA and a water non-solvent, and as a method for confirming the authenticity of a product, the presence or absence of amplification of ribonucleic acid by a PCR method. By confirming the above, it is only shown that the target product containing ribonucleic acid is identified.

In addition, the data on individual certification that enables the authentication of each product even if it is the same kind of product using the DNA sequence as the test index, as well as the authenticity confirmation data using the presence / absence of DNA as the test index. Not disclosed.

[0006] On the other hand, when a perpetrator escapes in an automobile accident, there is a demand for specifying a target vehicle from a piece of paint film, a piece of glass, a plastic piece, etc. left at the accident site.

[0007] The present invention has been made in view of the above-described problems of the prior art, and the purpose of the invention is to specifically identify the source / history product. It is an object of the present invention to provide a fluid fat composition containing an information nucleic acid.

Means for solving the problem

[0008] As a result of intensive studies to solve the above problems, the inventors of the present invention regard nucleic acid typified by DNA as one piece of authentication information, and detect information nucleic acid in products later. The present inventors have found that the above problems can be solved and have completed the present invention.

That is, the information nucleic acid-containing fluid fat composition of the present invention is a fluid oil composition containing an information nucleic acid having a site having an arbitrary and known base sequence.

The invention's effect

[0010] According to the present invention, even for a mass-produced product such as an industrial product, the target product can be individually authenticated by determining the base arrangement 1J of the information nucleic acid contained in a trace amount.

BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the information nucleic acid-containing fluid oil composition of the present invention will be described in detail. In addition

In the present specification and claims, “%” indicates mass percentage unless otherwise specified. “Fluid fat” means not solid fat, that is, a viscosity of 100 (mPa ′s) or less at a temperature of 30 ° C.

[0012] The information nucleic acid-containing fluid oil composition of the present invention as described above is a fluid oil composition containing an information nucleic acid having a site having an arbitrary and known base sequence. Here, as the oil and fat component, an ester-based or paraffin-based one that is not particularly limited can be used. Examples of the ester oil and fat component include a higher fatty acid and glycerin triester and a monoester of higher fatty acid and higher alcohol. Examples of the paraffin oil and fat component include paraffin having 15 or less carbon atoms. 'Skilling power S can.

Such a fluid oil composition can be used, for example, in oil, cosmetics, detergents, and the like, and can contain information nucleic acids that are difficult to remove, and thus can be individually and specifically authenticated.

[0013] Here, the information nucleic acid means DNA (deoxyribonucleic acid), RNA (ribonucleic acid) and derivatives thereof, which may be natural type or artificial type, but are included in a fluid oil composition with severe use environment. In view of this, it is preferable to use an artificial mold that is structurally stable. In the artificial type, a sequence that does not exist in the natural type can be formed.

In addition, in the information nucleic acid, the fact that the base sequence site is arbitrary indicates that it can be selected at random as long as it is a detectable base sequence, and that the base sequence site is known is used for individual authentication. It shows that the base sequence to be used is known beforehand.

[0014] In the present invention, the content of the information nucleic acid to be used is preferably a force S of 0.5 to 500 / ig relative to the composition lOOg, and more preferably a force S of 50 to 100. ,.

If less than 0.5, it is possible that the information nucleic acid is detected from the fluid oil composition and the base coordination IJ cannot be determined, and if it exceeds 500 / g, the information nucleic acid can be identified. The impact is not good, but the cost is high.

[0015] In the present invention, the size of the information nucleic acid is preferably such that the number of bases in the whole nucleic acid is 200 or less. If it exceeds 200, unreacted sites are generated little by little at the synthesis stage, and the content of those lacking bases easily increases. More preferably, it is about 100 bases.

Furthermore, it is preferable that thymines are not adjacent to each other in the above base sequence. As a result, dimerization of thymine can be suppressed.

In the present invention, the information nucleic acid is derived from a protective group from the viewpoint of improving stability when used in combination with a compound that reacts with a ◯ H group or when used in a harsh environment. It is preferable that Specifically, the hydroxyl group in one or both of the 5 and 3 groups is derivatized using a phosphate ester group, an acylol group, an alkoxycarbonyl group, a benzyl group, a substituted benzynole group and an aryl group. be able to. Fig. 1 (A) shows natural DNA, and (B) shows DNA derivatized at the 5 'position.

Furthermore, from the viewpoint of enhancing the convenience of isolation and purification, it is preferable to derivatize the hydroxyl group at the 5 ′ position with piotin or a fluorescent molecule. Specifically, when vithion is used, it is easily adsorbed selectively on a column to which a protein called avidin is bound. On the other hand, if a fluorescent molecule such as fluorescein is used, the nucleic acid itself becomes fluorescent, so that it can be detected with high sensitivity and purification becomes easy. In this way, individual authentication becomes extremely easy if the convenience of isolation and purification is enhanced.

[0017] Furthermore, for example, when the 5'-position is substituted with sulfur, the product extracted with water can be easily separated by passing it through a column of a carrier coated with gold (Au). When RNA is used as the information nucleic acid, two standing hydroxyl groups can be derivatized with the above protecting groups from the viewpoint of improving stability.

[0018] Further, in the case of performing individual authentication by extracting an information nucleic acid, from the viewpoint of efficiently detecting even a small content, the above base sequence site is a site used for amplification of the information nucleic acid It is preferable that As a powerful method for amplifying information nucleic acid, polymerase chain reaction (PCR) that can be amplified synergistically can be appropriately employed.

In the above PCR method, even if the amount of information nucleic acid is extremely small, it can be amplified extremely. For example, temperature control is performed in the presence of a base (primer) complementary to several tens of bases of DNA. When the thermostable DNA polymerase is allowed to act, the original DNA can be doubled. For example, if this is repeated 30 times, it can be amplified several hundred million times.

Such amplification makes it possible to obtain a sufficient amount of DNA to determine its sequence even from a very small amount of sample, and by extension, the “identity” of the product that contained it from the information corresponding to the sequence. Will be revealed.

[0019] At this time, it is preferable that the sites used for the amplification have primer corresponding sites necessary for polymerase chain reaction (PCR) at both ends. That is, as informational nucleic acid Although it is possible to use one that does not have a primer-corresponding site, it is possible to identify in a shorter time by providing a primer-corresponding site.

For such a primer corresponding site, the lower limit of the number of bases is preferably 5. More preferably, it is 10 or more. If the number of bases is less than 5, the number of distinguishable nucleic acids decreases, and it takes time to identify many products. On the other hand, the upper limit for the number of bases is preferably 100. This is because when the number of bases exceeds 100, the ratio of by-products lacking a base at any position increases, and purification takes time and tends to be difficult in some cases.

When RNA is used as the information nucleic acid, DNA complementary to the sequence can be obtained using reverse transcriptase, and PCR can be performed using this DNA.

[0020] Further, it is preferable that the information nucleic acid further has an authentication information part in addition to the base sequence part, so that individual authentication can be performed by setting more detailed information. For example, as shown in FIG. 2, in the case of information DNA having primer-corresponding sites at both ends, a sequence of m bases is placed in the center (B to B), and the sequence information of this portion is used as authentication information.

1 m

Correspond to the news. At both ends, sequences (X to X, P to P) complementary to 1 (el) and n primers are ligated, respectively. The presence of this part is the first time PCR

1 1 1 n

Adoption is possible.

The information DNA can be used as an information element as a single-stranded DNA or a double-stranded DNA complexed with a complementary sequence of DNA. This primer-corresponding site IJ can be devised so that the binding of complementary sequences is as stable as possible and amplification by the PCR method proceeds smoothly.

In FIG. 2, each of X to X, B to B, and P to P is deoxy.

1 1 1 m 1 n

It is composed of adenosine (dA), deoxyguanosine (dG), deoxycytosine (dC) or deoxythymine (dT) and any combination thereof.

[0021] As a suitable example of the above PCR method, in determining the base coordination IJ of the information nucleic acid contained in the fluid oil composition, the fluidized oil composition strength, the extracted nucleic acid solution, the PCR buffer solution, and the sterilization are determined. Mix distilled water, at least one primer, 2,3_dioxynucleoside triphosphate (dNTP) and polymerase, (1) heat at 92-95 °° for 2-5 minutes, then ( 2a) 92-95. (2b) 20-50 for 30-60 seconds at C. 30-60 seconds at C, (2c) 70-80. In C 30 ~: 120 ~ 50 times, after 20 ~ 50 times of the heat cycle, (3) 70 ~ 80. Heat treatment is preferably performed for 1 to 10 minutes. It is preferable to use two types of primers from the viewpoint of increasing the arbitraryness of the base sequence of the information nucleic acid.

[0022] In (1), 5 minutes at 94 ° C is particularly preferred. If it is shorter than 2 minutes at 92 ° C, it will be difficult to separate the DNA into two strands. If it is longer than 5 minutes at 95 ° C, the enzyme will be inactivated. Note that this is not necessary when the information nucleic acid strength S1 strand contained in the product is used.

In (2a), 30 seconds at 94 ° C is particularly preferred. This is because the amplification factor decreases when the temperature is shorter than 30 seconds at 92 ° C, and the enzyme is deactivated when the temperature is longer than 60 seconds at 95 ° C.

In (2b), 30 seconds at 40 ° C is particularly preferred. If it is shorter than 30 seconds at 20 ° C, it will be difficult to bind the primer and DNA, and if it is longer than 60 seconds at 50 ° C, the enzyme will be deactivated. Also, in (2c), 30 seconds at 72 ° C Is particularly preferred. If it is shorter than 30 seconds at 70 ° C, the elongation becomes insufficient, and if it is longer than 120 seconds at 80 ° C, the enzyme is inactivated.

Further, in (3), 7 minutes at 72 ° C is particularly preferable. If it is shorter than 1 minute at 70 ° C, the elongation will be insufficient, and if it is longer than 10 minutes at 80 ° C, time will be wasted.

Furthermore, the repetition of the heating cycles (2a) to (2c) is because the amplification factor decreases if 30 times is less than 20 times, which is particularly preferable, and if it exceeds 50 times, time is wasted.

[0023] FIG. 3 shows a flow chart of an embodiment of a method for detecting an information nucleic acid contained in an information nucleic acid-containing fluid oil composition.

As shown in the figure, in S1, information DNA is extracted from the fluid oil composition. In S2, concentrate by lyophilization. In S3, add two types of primers and polymerase. In S4, DNA is amplified by repeating PCR. In S5, the remaining excess primer is degraded by single-stranded DNA cleavage enzyme. In S6, double-stranded information DNA is purified by gel filtration. In S7, the sequencer determines the sequence.

[0024] Here, in S1, for example, when the fluid oil composition is mixed with a small amount of water, for example, when the information DNA is chemically bound to the fine particles, it is chemically bonded. Hydrolysis Etc., it can be extracted efficiently. In S2, it may be concentrated using, for example, a centrifugal evaporator. Further, in S5, for example, Taq DNA polymerase, Tth DNA polymerase, Tfl DNA polymerase, Vent polymerase, Pfu polymerase, Bca BEST polymerase, KOD DNA polymerase, etc. can be used as the single-stranded DNA cleavage enzyme. Further, the target DNA may be amplified between S6 and S7 by repeating the same operations as in S3 and 4. In S7, the sequence determination may be performed by a mass spectrometer, or may be combined with the sequence determination by a sequencer.

[0025] Further, in determining the base sequence, it is desirable to compare the information nucleic acid data extracted from the product with the information nucleic acid database including at least the information nucleic acid data. This is because the time required for product certification can be significantly reduced by comparing with a database of information nucleic acids obtained in advance.

Examples of data stored in such a database include the electrophoresis time and the distance traveled by gel filtration (this is sufficient if the information nucleic acid itself is allowed to flow into the control lane).

[0026] Further, in the present invention, it is desirable that the contained information nucleic acid is supported on fine particles.

Here, in the present invention, “supporting” means that the information nucleic acid is in close contact with the fine particles so that they can move together with the fine particles, and the information nucleic acids are attached to the surfaces of the fine particles. Even if it is firmly fixed, it may adhere to the surface of the fine particles or in the recesses with a strength sufficient to withstand use.

Since the nucleic acid is water-soluble, it cannot be said that there is no possibility that it will flow out of the product together with water even if it is incorporated in the product.

[0027] The fine particles are not particularly limited as long as they can carry an information nucleic acid, but in addition to silica and zinc oxide, titanium oxide, molybdenum oxide, tungsten oxide, noble titanate and the like are preferable. Can be used. In addition, the dispersibility at the time of mixing with fluid oil-fat composition can be improved by carrying out the silica coat of the surface.

[0028] In the present invention, the content of the fine particles is 0.5 with respect to the total amount of the composition. It is preferably ˜5%, more preferably 0.5 to 1%.

When the content of the fine particles is less than 0.5%, the amount of fine particles in the sampled fluid oil composition may be small, and the detection accuracy may be lowered. On the other hand, when it exceeds 5%, in the case of oil, it tends to agglomerate and sedimentation occurs. In the case of cosmetics, it may damage the skin.

[0029] Further, as the size of the fine particles, the average particle size is preferably 0.01-1 μm, more preferably 0.02-0.lzm.

If the average particle size of the fine particles is less than 0.01 x m, the identification accuracy of the information nucleic acid decreases. If it exceeds 1 z m, in the case of oil, it tends to aggregate and sedimentation occurs. In the case of cosmetics, the skin may be damaged.

The information nucleic acid-carrying fine particles are formed by, for example, dissolving the information nucleic acid as it is in a suspension obtained by adding sterile distilled water to the fine particles, or by dissolving a part or all of the information nucleic acid in sterile distilled water. It can be obtained by adding as a solution, desirably adding a specific solvent, and drying.

An information nucleic acid with a site having an arbitrary and known base sequence directly in a suspension of fine particles such as silica and zinc oxide, or a solution of a part or all of the information nucleic acid dissolved in sterile distilled water The information nucleic acid can be reliably supported on the surface of the fine particles by drying after being held in a state, and the information nucleic acid is supported on the fine particles by using such information nucleic acid-supported fine particles. Introducing into the fluid oil composition in a state allows the information nucleic acid to be added to the fluid oil composition more reliably and stably dispersed than when introduced in the state of the information nucleic acid alone. The ability to prevent the outflow of information nucleic acid due to moisture can be prevented, and it is extremely possible that individual verification of the source and history of products using the base sequence of information nucleic acid as a detection index will be possible over a long period of time. It is hit the effect force.

[0031] In addition, the suspension includes alcohol (eg, methanol, ethanol, propanol, butanol, pentanol, hexanol, heptanol, octanol, nonanol, etc.), ester (eg, ethyl acetate, butyl acetate, Propyl acetate, etc.), ketones (eg, acetone, dimethyl ketone, methyl ethyl ketone, jetyl ketone, etc.) and aromatic solvents ( It is desirable to further add a solvent such as toluene, hexane, cyclohexane, xylene, etc. This improves the dispersibility of the fine particles in the suspension, and after the addition of the information nucleating acid. The volatilization of the water and solvent will be promoted.

These solvents are not limited to only one type, and two or more types of solvents can be used in combination. These solvents may be added at the same time as the information nucleic acid or after the information nucleic acid is added.

[0032] Further, as the amount of solvent added, in the case of alcohol, it is preferable that the volume ratio of sterilized distilled water Z alcohol is in the range of 1 to 99, that is, a solvent other than alcohol, that is, an ester. In the case of a ketone or an aromatic solvent, the volume ratio of sterile distilled water Z solvent is preferably in the range of 1 to 75.

That is, if the addition amount of the solvent is too small, the above effect due to the addition of the solvent cannot be sufficiently obtained. Conversely, if the addition amount is too large, the water tends to remain without volatilization due to a decrease in compatibility with water. There is a tendency to become insufficient.

Example

Hereinafter, the present invention will be described in more detail with reference to several examples, but the present invention is not limited to these examples.

[Example 1]

[Preparation of information nucleic acid-supported fine particles]

Informational DNA represented by SEQ ID NO: 1 (including a verifiable DNA represented by SEQ ID NO: 2) was synthesized by sequentially binding nucleosides by the phosphoramidite method.

Hereinafter, in Examples 1 to 20, an information DNA represented by SEQ ID NO: 1 was used as the information nucleic acid.

Zinc oxide with an average particle diameter of 0.02 xm (ZS-032 manufactured by Showa Denko KK) coated with silica was used as fine particles for the carrier, and 30% ethanol by volume ratio was added to 4 g of the zinc oxide. 15 mL of sterilized distilled water contained was added and stirred to obtain a zinc oxide suspension.

10 nmol (164 μg) of informational DNA was dissolved in 15 mL of sterile distilled water, and the resulting informational aqueous DNA solution was added to the suspension and stirred well. Further, 4 mL of ethanol was added and stirred. The suspension containing the computerized DNA was poured into a plastic circular dish and air-dried for two days with the top covered with air-permeable paper or cloth. Further, the almost dried product was put in a desiccator and dried under reduced pressure under heating at 40 ° C. to be sufficiently dried.

The fully dried lump fine powder was put in a mortar and crushed to obtain information nucleic acid-carrying fine particles as the original fine powder.

[0035] [Preparation of fluidized fat composition containing information nucleic acid]

20g of information nucleic acid-carrying fine particles and lOOOg oil (Elf, Evolution SXR

5W30) was mixed to produce the fluidized oil / fat composition (oil) containing the information nucleic acid of this example.

[0036] (Examples 2 to 20)

As fine particles for the carrier, silica-coated zinc oxide with an average particle size of 0.02 xm (ZS-032 from Showa Denko KK), silica with an average particle size of 1 μm (manufactured by Suzuki Yushi Kogyo Co., Ltd.) 2C) Use silica with an average particle diameter of 4 μm (E-16C, manufactured by Suzuki Oil & Fat Co., Ltd.) to adjust the content of the information nucleic acid supported on the fine particles, or The content was adjusted to produce an information nucleic acid-containing fluid oil composition (oil) of this example. Tables 1 and 2 show the specifications of the information nucleic acid-containing fluid oil composition (oil) in each of the above examples.

[0037] [Table 1]

[0038] [Table 2]

[0039] [Evaluation test] (Preparation of test piece)

(1) Zinc phosphate-treated 70111111 150111111 0.8mmt dull steel plate with cationic electrodeposition paint (Nippon Paint Co., Ltd., trade name "Power Top U600M") to a dry film thickness of 20 zm After the electrodeposition coating, it was baked at 160 ° C for 30 minutes to form an undercoat layer, and then a gray paint (made by NOF Corporation, trade name “HIEIPICO No. 500”) was applied thereon. The coating was applied to a dry film thickness of 30 xm and baked at 140 ° C for 30 minutes to form an intermediate coating layer and a base coat layer.

On this base coat layer, Talia paint (made by Nippon Paint Co., Ltd., trade name “Super Parrak O-130 GN3”) was applied so that the dry film thickness was 30 zm, and the coating was performed at 140 ° C. A talya layer was formed by baking for 0 minutes.

(2) The information nucleic acid-containing fluid oil composition (oil) of each of the above examples was applied to the surface of the coating film to form a wax film with a thickness of 100 μm to prepare a test piece.

(Identification of information nucleic acid)

(1) The information nucleic acid-containing fluid fat composition (oil) solidified from the test pieces of each of the above examples was removed, 5 mL of sterile distilled water was added, and the mixture was stirred with a magnetic stirrer to extract DNA into the aqueous layer. .

(2) The oil and the aqueous layer were separated using a centrifuge, and the aqueous layer was concentrated using a centrifugal evaporator.

(3) Add the eluted and recovered DNA solution (5 μL) to the PCR buffer (5 μL), Taq

polymerase (0. 25 / i L), sterile distilled water (24 · 75 μL), 5 β represented by SEQ ID NO: 3

Amber primer 1 (5 / i L), 5 μΜ primer 2 (5 / i L) represented by SEQ ID NO: 4 and 2 mM dNTP (5 μL) were mixed.

(4) After heating at 94 ° C for 5 minutes, the cycle of [heating at 94 ° C for 30 seconds → heating at 40 ° C for 30 seconds → heating at 72 ° C for 30 seconds] was repeated 30 times.

(5) After heat treatment at 72 ° C for 7 minutes, it was stored at 4 ° C.

(6) Using a single-strand DNA cleaving enzyme (S1 nuclease (reacts specifically with single-strand DNA)), the excess primer was decomposed and the target double-strand information DNA was purified by gel filtration.

(7) One kind of primer on purified information DNA (Primer 1 represented by SEQ ID NO: 3) And fluorescently labeled 2,3-dideoxynucleoside triphosphate.

(8) The same operations as in the above steps (3) to (5) were performed.

(9) After gel filtration purification, it was subjected to sequencing using an automatic sequencer.

[0041] The results obtained are also shown in Tables 1 and 2. In the table “Identification of computerized DNA”, “

“O” indicates that the information nucleic acid was detected by the above-described detection method, and “△” indicates P

Except for increasing the number of CR treatments, those that could be identified by the same operation as the above-mentioned method for detecting information nucleic acids.

[0042] (Evaluation of sedimentation)

The fluidized oil / fat composition containing information nucleic acid (oil) in each of the above examples was stirred and allowed to stand for 2 hours, and the state of the oil was observed. The results obtained are shown in Tables 1 and 2. In the table, “O” indicates “no sediment”, “△” indicates “slight sediment”, and “X” indicates “many sediments”.

[0043] From Table 1 and Table 2, it can be seen that the information nucleic acid-containing fluid oil composition of the present invention can identify the information nucleic acid.

In addition, the information-containing nucleic acid-containing fluid fat composition of Examples 12 to 12 and Examples 18 to 20 is less in number than the information-containing nucleic acid-containing fluid fat composition of Examples 13 to 17 and PCR. It can be seen that the information nucleic acid can be identified by the frequency of amplification.

Furthermore, it can be seen that the information nucleic acid-containing fluid oil compositions of Examples:! To 17 are excellent in the dispersion stability of the contained information-forming nucleic acid.

Brief Description of Drawings

[0044] FIG. 1 is a structural formula showing natural DNA and DNA in which the 5 ′ position is derivatized.

FIG. 2 is a schematic diagram showing an information nucleic acid having primer-corresponding sites at both ends at the authentication information site.

FIG. 3 is a flowchart showing one embodiment of a method for detecting information nucleic acid contained in a fluidized fat composition containing information nucleic acid.

Claims

The scope of the claims

[1] An information nucleic acid-containing fluid oil composition comprising an information nucleic acid having a site having an arbitrary and known base sequence.

[2] The information nucleic acid-containing fluid oil composition according to claim 1, wherein the content of the information nucleic acid is 0.5 to 500 μg with respect to 100 g of the composition.

[3] The oil or fat component is ester-based or paraffin-based, or

2. The fluidized oil / fat composition containing the information nucleic acid according to 2.

[4] The information nucleic acid-containing fluid oil composition according to any one of claims 1 to 3, wherein the information nucleic acid is supported on fine particles.

[5] The information nucleic acid-containing fluid oil composition according to claim 4, wherein the average particle diameter of the fine particles is 0.01 to 1 μm.

[6] The information nucleic acid-containing fluid oil composition according to claim 4 or 5, wherein the content of the fine particles is 0.5 to 5% with respect to the total amount of the composition.

[7] An information nucleic acid-containing fluid / oil composition according to any one of claims 1 to 6, wherein the composition is used for at least one selected from the group consisting of oil, cosmetics and detergent power. object.