WO2006060376A3 - Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase - Google Patents

Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase Download PDF

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Publication number
WO2006060376A3
WO2006060376A3 PCT/US2005/043097 US2005043097W WO2006060376A3 WO 2006060376 A3 WO2006060376 A3 WO 2006060376A3 US 2005043097 W US2005043097 W US 2005043097W WO 2006060376 A3 WO2006060376 A3 WO 2006060376A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
polynucleotide
stress
trehalose
kernels
Prior art date
Application number
PCT/US2005/043097
Other languages
French (fr)
Other versions
WO2006060376A2 (en
Inventor
Mark L Lagrimini
Michael Nuccio
Natasha Springer
Original Assignee
Syngenta Participations Ag
Mark L Lagrimini
Michael Nuccio
Natasha Springer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag, Mark L Lagrimini, Michael Nuccio, Natasha Springer filed Critical Syngenta Participations Ag
Priority to AU2005312023A priority Critical patent/AU2005312023A1/en
Priority to CA002588372A priority patent/CA2588372A1/en
Priority to MX2007006452A priority patent/MX2007006452A/en
Priority to EP05849969A priority patent/EP1827081A4/en
Priority to BRPI0518596-3A priority patent/BRPI0518596A2/en
Publication of WO2006060376A2 publication Critical patent/WO2006060376A2/en
Publication of WO2006060376A3 publication Critical patent/WO2006060376A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Abstract

The present invention relates to transgenic plants comprising an isolated DNA molecule comprising a polynucleotide that encodes a nucleic acid that down-regulates an endogenous T6PP gene, wherein the polynucleotide is under the control of a promoter that is stress-inducible and is expressed predominantly in vegetative tissue. The promoter may also be developmentally expressed in maturing kernels. Expression of the polynucleotide results in the increased availability of carbon to developing florets/kernels when plants are subject to environmental stress, such as a water deficit. The DNA molecule of the invention thereby permits more photosynthate to be directed to the developing ovules/embryos resulting in stabilized yield in growing environments that are subject to periodic stress.
PCT/US2005/043097 2004-12-03 2005-11-28 Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase WO2006060376A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2005312023A AU2005312023A1 (en) 2004-12-03 2005-11-28 Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase
CA002588372A CA2588372A1 (en) 2004-12-03 2005-11-28 Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase
MX2007006452A MX2007006452A (en) 2004-12-03 2005-11-28 Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase.
EP05849969A EP1827081A4 (en) 2004-12-03 2005-11-28 Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase
BRPI0518596-3A BRPI0518596A2 (en) 2004-12-03 2005-11-28 stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63278104P 2004-12-03 2004-12-03
US60/632,781 2004-12-03

Publications (2)

Publication Number Publication Date
WO2006060376A2 WO2006060376A2 (en) 2006-06-08
WO2006060376A3 true WO2006060376A3 (en) 2007-05-03

Family

ID=36565624

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/043097 WO2006060376A2 (en) 2004-12-03 2005-11-28 Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase

Country Status (8)

Country Link
EP (1) EP1827081A4 (en)
CN (1) CN101115385A (en)
AU (1) AU2005312023A1 (en)
BR (1) BRPI0518596A2 (en)
CA (1) CA2588372A1 (en)
MX (1) MX2007006452A (en)
WO (1) WO2006060376A2 (en)
ZA (1) ZA200704131B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008074025A2 (en) * 2006-12-13 2008-06-19 Idiverse, Inc. Novel genes and rna molecules that confer stress tolerance
EP2468873A1 (en) * 2006-12-15 2012-06-27 CropDesign N.V. Plants having enhanced yield-related traits and a method for making the same
BRPI0720084A2 (en) * 2006-12-15 2014-01-21 Cropdesign Nv METHODS FOR INCREASED SEED YIELD IN PLANTS IN RELATION TO CONTROL PLANTS, AND FOR THE PRODUCTION OF A TRANSGENIC PLANT, PLANT, PART OF THE PLANT PLANT OR CELL, INSULATED NUCLEIC ACID MOLECULES, ISOLATED CONDUCT, ISOLATED CONDUCT NUCLEIC ACID, COLLECTABLE PARTS OF A PLANT, AND PRODUCTS
CA2759007A1 (en) * 2009-04-24 2010-10-28 Australian Centre For Plant Functional Genomics Pty Ltd Drought responsive expression of genes from the zea mays rab17 promoter
CN110643627A (en) * 2018-06-25 2020-01-03 中国农业大学 CIPK3 protein and application of coding gene thereof in drought resistance of plants
CN110358776B (en) * 2019-07-09 2021-03-12 华南农业大学 Rhizoctonia solani pathogenic related gene and application thereof
CN116694659B (en) * 2023-05-26 2024-02-02 中国农业科学院生物技术研究所 Application of GhTPPA2 gene in promoting accumulation of soluble sugar and starch in plant leaves

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6686516B2 (en) * 1998-03-11 2004-02-03 Syngenta Participations Ag Expression of trehalose 6-phosphate synthase in plant plastids
US20040034888A1 (en) * 1999-05-06 2004-02-19 Jingdong Liu Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN1997CH00924A (en) * 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate
ZA989886B (en) * 1997-10-30 1999-04-30 Mogen Int Nuclear male sterile plants method of producing same and methods to restore fertility
WO2002010365A2 (en) * 2000-08-02 2002-02-07 The Board Of Regents Of The University Of Nebraska Down-regulation of single genes and simultaneous down-regulation of multiple genes by nuclear localization of rna transcripts
EP1375669A1 (en) * 2002-06-13 2004-01-02 Stichting Voor De Technische Wetenschappen Method for enhancing the disease resistance in plants by altering trehalose-6-phosphate levels
US20040123347A1 (en) * 2002-12-20 2004-06-24 Hinchey Brendan S. Water-deficit-inducible plant promoters

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6686516B2 (en) * 1998-03-11 2004-02-03 Syngenta Participations Ag Expression of trehalose 6-phosphate synthase in plant plastids
US20040034888A1 (en) * 1999-05-06 2004-02-19 Jingdong Liu Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of EP1827081A4 *
VILARDELL J. ET AL.: "Regulation of the maize rab17 gene promoter in transgenic heteroologous systems", PLANT MOLECULAR BIOLOGY, vol. 17, 1991, pages 985 - 993 *

Also Published As

Publication number Publication date
WO2006060376A2 (en) 2006-06-08
CA2588372A1 (en) 2006-06-08
ZA200704131B (en) 2008-08-27
MX2007006452A (en) 2007-07-19
BRPI0518596A2 (en) 2008-11-25
EP1827081A4 (en) 2009-04-01
CN101115385A (en) 2008-01-30
AU2005312023A1 (en) 2006-06-08
EP1827081A2 (en) 2007-09-05

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