BRPI0518596A2 - stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase - Google Patents

stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase

Info

Publication number
BRPI0518596A2
BRPI0518596A2 BRPI0518596-3A BRPI0518596A BRPI0518596A2 BR PI0518596 A2 BRPI0518596 A2 BR PI0518596A2 BR PI0518596 A BRPI0518596 A BR PI0518596A BR PI0518596 A2 BRPI0518596 A2 BR PI0518596A2
Authority
BR
Brazil
Prior art keywords
plants
stress
selective inhibition
phosphate phosphatase
polynucleotide
Prior art date
Application number
BRPI0518596-3A
Other languages
Portuguese (pt)
Inventor
Mark L Lagrimini
Natasha Springer
Michael Nuccio
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of BRPI0518596A2 publication Critical patent/BRPI0518596A2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Abstract

TOLERÂNCIA AO ESTRESSE EM PLANTAS ATRAVÉS DA INIBIÇçO SELETIVA DA TREALOSE-6-FOSFATO FOSFATASE. A presente invenção se refere a plantas transgênicas que compreendem uma molécula de DNA isolada compreendendo um polinucleotideo que codifica um ácido nuclélco que regula para menos um gene T6PP endógeno, em que o polinucleotideo está sob o controle de um promotor que pode ser induzido por estresse e é expresso predominantemente no tecido vegetativo. O promotor pode ainda ser expresso em relação ao desenvolvimento em grãos em maturação. A expressão do polinucleotídeo resulta na maior disponibilidade de carbono aos florícuíos/grãos quando as plantas são submetidas ao estresse ambiental, tal como um déficit hídrico. A molécula de DNA da invenção permite assim que mais produto da fotossíntese seja direcionado para os óvulos/embriões em desenvolvimento resultando no rendimento estabilizado nos ambientes de cultivo que estão sujeitos ao estresse periódico.TOLERANCE TO STRESS IN PLANTS THROUGH SELECTIVE INHIBITION OF TREALOSE-6-PHOSPHATE PHOSPHATASE. The present invention relates to transgenic plants comprising an isolated DNA molecule comprising a polynucleotide encoding a nucleic acid that down-regulates an endogenous T6PP gene, wherein the polynucleotide is under the control of a stress-inducible promoter. is predominantly expressed in vegetative tissue. The promoter may further be expressed in relation to growth in maturing grains. Polynucleotide expression results in greater carbon availability to floricui / grains when plants are subjected to environmental stress, such as a water deficit. The DNA molecule of the invention thus allows more photosynthesis product to be directed to developing eggs / embryos resulting in stabilized yield in culture environments that are subject to periodic stress.

BRPI0518596-3A 2004-12-03 2005-11-28 stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase BRPI0518596A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63278104P 2004-12-03 2004-12-03
PCT/US2005/043097 WO2006060376A2 (en) 2004-12-03 2005-11-28 Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase

Publications (1)

Publication Number Publication Date
BRPI0518596A2 true BRPI0518596A2 (en) 2008-11-25

Family

ID=36565624

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0518596-3A BRPI0518596A2 (en) 2004-12-03 2005-11-28 stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase

Country Status (8)

Country Link
EP (1) EP1827081A4 (en)
CN (1) CN101115385A (en)
AU (1) AU2005312023A1 (en)
BR (1) BRPI0518596A2 (en)
CA (1) CA2588372A1 (en)
MX (1) MX2007006452A (en)
WO (1) WO2006060376A2 (en)
ZA (1) ZA200704131B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2121921A4 (en) * 2006-12-13 2010-08-04 Idiverse Inc Novel genes and rna molecules that confer stress tolerance
US20100024067A1 (en) * 2006-12-15 2010-01-28 Cropdesign N.V. Plants having enhanced seed yield-related traits and a method for making the same
BRPI0720084A2 (en) * 2006-12-15 2014-01-21 Cropdesign Nv METHODS FOR INCREASED SEED YIELD IN PLANTS IN RELATION TO CONTROL PLANTS, AND FOR THE PRODUCTION OF A TRANSGENIC PLANT, PLANT, PART OF THE PLANT PLANT OR CELL, INSULATED NUCLEIC ACID MOLECULES, ISOLATED CONDUCT, ISOLATED CONDUCT NUCLEIC ACID, COLLECTABLE PARTS OF A PLANT, AND PRODUCTS
US8766037B2 (en) 2009-04-24 2014-07-01 Australian Centre For Plant Functional Genomics Pty Ltd Drought responsive expression of genes from the Zea mays Rab17 promoter
CN110643627A (en) * 2018-06-25 2020-01-03 中国农业大学 CIPK3 protein and application of coding gene thereof in drought resistance of plants
CN110358776B (en) * 2019-07-09 2021-03-12 华南农业大学 Rhizoctonia solani pathogenic related gene and application thereof
CN116694659B (en) * 2023-05-26 2024-02-02 中国农业科学院生物技术研究所 Application of GhTPPA2 gene in promoting accumulation of soluble sugar and starch in plant leaves

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN1997CH00924A (en) * 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate
ZA989886B (en) * 1997-10-30 1999-04-30 Mogen Int Nuclear male sterile plants method of producing same and methods to restore fertility
BR9909258A (en) * 1998-03-11 2000-11-28 Novartis Ag Expression of trehalose biosynthetic genes in plants
US20100293669A2 (en) * 1999-05-06 2010-11-18 Jingdong Liu Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement
WO2002010365A2 (en) * 2000-08-02 2002-02-07 The Board Of Regents Of The University Of Nebraska Down-regulation of single genes and simultaneous down-regulation of multiple genes by nuclear localization of rna transcripts
EP1375669A1 (en) * 2002-06-13 2004-01-02 Stichting Voor De Technische Wetenschappen Method for enhancing the disease resistance in plants by altering trehalose-6-phosphate levels
US20040123347A1 (en) * 2002-12-20 2004-06-24 Hinchey Brendan S. Water-deficit-inducible plant promoters

Also Published As

Publication number Publication date
CN101115385A (en) 2008-01-30
ZA200704131B (en) 2008-08-27
AU2005312023A1 (en) 2006-06-08
EP1827081A2 (en) 2007-09-05
WO2006060376A2 (en) 2006-06-08
WO2006060376A3 (en) 2007-05-03
EP1827081A4 (en) 2009-04-01
MX2007006452A (en) 2007-07-19
CA2588372A1 (en) 2006-06-08

Similar Documents

Publication Publication Date Title
BRPI0518596A2 (en) stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase
Yartseva et al. The maternal-to-zygotic transition during vertebrate development: a model for reprogramming
Fan et al. Transcriptome-wide identification of salt-responsive members of the WRKY gene family in Gossypium aridum
Kato et al. A 5′ UTR-overlapping LncRNA activates the male-determining gene doublesex1 in the crustacean Daphnia magna
BR112018011242A2 (en) methods for genetic modification of plants
BR112012023808A2 (en) Methods for producing a phytase variant, for enhancing the nutritional value of an animal feed, for treating protein, for synthesizing a fermentation product, for producing ethanol, polynucleotide, nucleic acid construct, recombinant expression vector, host cell recombinant, transgenic plant, composition, process to reduce phytase levels in animal manure, and use of phytase variant
BRPI0507573A (en) transgenic corn seed with enhanced amino acid content
BR112013010238A2 (en) vegetables, vegetable seed, methods of identifying a dtp6 protein, to increase and assess drought tolerance, to determine a change in biomass yield, to increase triple strain, strain and paraquat tolerance in a plant and isolated polynucleotide
BR112017007932A2 (en) insecticide proteins and methods for their use
AR066451A1 (en) COMBINATIONS OF NUCLEIC ACID MOLECULES THAT CODIFY SUBUNITIES OF PIRUVATO QUINASA AND METHODS THAT USE THEM IN THE IMPROVEMENT OF SEEDS
Espada Non-catalytic functions of DNMT1
BR112013006043A2 (en) target gene for control and use of phytoparasitic nematodes.
AR065282A1 (en) COMPOSITIONS AND METHODS THAT USE CAD TYPE TRANSFER RNA FOR NEMATODE CONTROL
BR112012023822B8 (en) methods to produce a phytase variant, to improve the nutritional value of an animal feed, to produce a fermentation product and to produce ethanol, phytase variant, composition, and, use of the phytase variant or composition
Wang et al. Genome-wide analysis and expression profiling of the phospholipase C gene family in soybean (Glycine max)
BRPI0922980A8 (en) METHOD TO ACCELERATE PLANT GROWTH AND IMPROVE PRODUCTION BY INTRODUCING PHOSPHATASE INTO TRANSGENIC PLANT
BR112017019194A2 (en) Structure-based methods for modifying pip-72 polypeptides and pip-72 polypeptides derived therefrom
AR090204A1 (en) BACILIFORM VIRAL BOX OF SUGAR CANE (SCBV) AND ITS USE IN THE FUNCTIONAL GENOMICS OF PLANTS
BR112012009044A2 (en) Isolated nucleic acid molecule, expression cassette, vector, plant cell, plant, transgenic seed, method for expressing a polynucleotide in a plant or plant cell and method for expressing a polynucleotide, preferably in somatic egg tissues of a plant
Xie et al. RNAi knockdown of dRNaseZ, the Drosophila homolog of ELAC2, impairs growth of mitotic and endoreplicating tissues
Ornelas-Ayala et al. The epigenetic faces of ULTRAPETALA1
BRPI0820004A8 (en) ISOLATED NUCLEIC ACID MOLECULE, EXPRESSION CASSETTE, VECTOR, METHOD FOR OBTAINING A PLANT CELL, METHOD FOR OBTAINING A PLANT, METHOD FOR EXPRESSING A POLYNUCLEOTIDE IN A PLANT OR PLANT CELL, METHOD FOR EXPRESSING A POLYNUCLEOTIDE PREFERENTIALLY IN REPRODUCTIVE TISSUES OF A PLANT
Li et al. Molecular characterization and function analysis of the rice OsDUF946 family
Permpoon et al. Isolation and characterization of Doublesex homologues in the Bactrocera species: B. dorsalis (Hendel) and B. correcta (Bezzi) and their putative promoter regulatory regions
BR112019005600A2 (en) plant promoter for transgene expression

Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]