WO2006052554A3 - Eucalyptus urophylla transformation and selection - Google Patents

Eucalyptus urophylla transformation and selection Download PDF

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Publication number
WO2006052554A3
WO2006052554A3 PCT/US2005/039520 US2005039520W WO2006052554A3 WO 2006052554 A3 WO2006052554 A3 WO 2006052554A3 US 2005039520 W US2005039520 W US 2005039520W WO 2006052554 A3 WO2006052554 A3 WO 2006052554A3
Authority
WO
WIPO (PCT)
Prior art keywords
explants
urophylla
transformation
selection
grandis
Prior art date
Application number
PCT/US2005/039520
Other languages
French (fr)
Other versions
WO2006052554A2 (en
Inventor
Shujun Chang
Robert D Thomas
Levis W Handley
Marie B Connett
Randy L Hamilton
Original Assignee
Arborgen Llc
Shujun Chang
Robert D Thomas
Levis W Handley
Marie B Connett
Randy L Hamilton
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/981,742 external-priority patent/US20060101536A1/en
Application filed by Arborgen Llc, Shujun Chang, Robert D Thomas, Levis W Handley, Marie B Connett, Randy L Hamilton filed Critical Arborgen Llc
Priority to BRPI0517531-3A priority Critical patent/BRPI0517531A2/en
Priority to EP05851291A priority patent/EP1814379A4/en
Priority to JP2007540378A priority patent/JP4928461B2/en
Priority to AU2005305071A priority patent/AU2005305071A1/en
Publication of WO2006052554A2 publication Critical patent/WO2006052554A2/en
Priority to IL182777A priority patent/IL182777A0/en
Publication of WO2006052554A3 publication Critical patent/WO2006052554A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention relates to a method for transforming and selecting plant explants. The transformation method includes pre-culturing the explants in the presence of an Agrobacterium inducer and exposing the transformed explants to a shoot regeneration media that accelerates shoot development. Plants generated from this transformation method are provided. In particular, methods for obtaining transgenic E. grandis x E. urophylla cells and therefrom regenerating stably transformed E. grandis x E. urophylla trees are provided. The invention also provides media, methods, and plasmids for selecting and regenerating plants.
PCT/US2005/039520 2004-11-05 2005-11-03 Eucalyptus urophylla transformation and selection WO2006052554A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
BRPI0517531-3A BRPI0517531A2 (en) 2004-11-05 2005-11-03 Methods of transformation of at least one cell of an e explant. grandis x e. urophylla with strange DNA and Tree production e. grandis x e. chimeric urophylla
EP05851291A EP1814379A4 (en) 2004-11-05 2005-11-03 Eucalyptus urophylla transformation and selection
JP2007540378A JP4928461B2 (en) 2004-11-05 2005-11-03 Transformation and selection of Eucalyptus urophylla
AU2005305071A AU2005305071A1 (en) 2004-11-05 2005-11-03 Eucalyptus urophylla transformation and selection
IL182777A IL182777A0 (en) 2004-11-05 2007-04-25 Method for transforming and selecting eucalyptus urophylla

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10/981,742 US20060101536A1 (en) 2004-11-05 2004-11-05 Eucalyptus urophylla transformation and regeneration
US10/981,742 2004-11-05
US11/158,342 2005-06-22
US11/158,342 US20060101537A1 (en) 2004-11-05 2005-06-22 Eucalyptus urophylla transformation and selection

Publications (2)

Publication Number Publication Date
WO2006052554A2 WO2006052554A2 (en) 2006-05-18
WO2006052554A3 true WO2006052554A3 (en) 2008-09-04

Family

ID=36336974

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/039520 WO2006052554A2 (en) 2004-11-05 2005-11-03 Eucalyptus urophylla transformation and selection

Country Status (4)

Country Link
EP (1) EP1814379A4 (en)
AR (1) AR051478A1 (en)
AU (1) AU2005305071A1 (en)
WO (1) WO2006052554A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102480926A (en) * 2009-05-07 2012-05-30 阿博根公司 Materials and methods for regeneration and transformation of trees
CN103155872B (en) * 2013-04-12 2014-01-08 湛江师范学院 Eucalyptus urophylla*grandis embryoid induction seedling raising method
CN113430222A (en) * 2021-07-27 2021-09-24 中国林业科学研究院热带林业研究所 Eucalyptus transgenic method based on agrobacterium rhizogenes mediation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002525062A (en) * 1998-09-15 2002-08-13 ジェネシス リサーチ アンド デベロップメント コーポレイション リミテッド Method for producing genetically modified plants, and genetically modified plants, plant materials and plant products produced thereby
JP4099898B2 (en) * 1999-05-07 2008-06-11 王子製紙株式会社 Methods for transforming adult Eucalyptus plants
CA2528408A1 (en) * 2003-06-06 2004-12-16 Arborgen, Llc Plant transformation and selection

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GONZALEZ E.R. ET AL.: "Production of transgenic Eucalyptus grandis xE urophylla using the sonication-assisted Agobacterium transformation (SAAT) system", FUNCTIONAL PLANT BIOLOGY JOURNAL, vol. 29, no. 1, 2002, pages 97 - 102, XP009043070 *
See also references of EP1814379A4 *
TOURNIER ET AL.: "An efficient procedure to stably introduce genes into an economically important pulp tree (Eucalyptus grandis x Eucalyptus urophylla)", TRANSGENIC RESEARCH, vol. 12, August 2003 (2003-08-01), pages 403 - 411 *

Also Published As

Publication number Publication date
EP1814379A4 (en) 2009-02-25
WO2006052554A2 (en) 2006-05-18
EP1814379A2 (en) 2007-08-08
AU2005305071A1 (en) 2006-05-18
AR051478A1 (en) 2007-01-17

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