WO2006041938A2 - Micropatterned plate with micro-pallets for addressable biochemical analysis - Google Patents
Micropatterned plate with micro-pallets for addressable biochemical analysis Download PDFInfo
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- WO2006041938A2 WO2006041938A2 PCT/US2005/035860 US2005035860W WO2006041938A2 WO 2006041938 A2 WO2006041938 A2 WO 2006041938A2 US 2005035860 W US2005035860 W US 2005035860W WO 2006041938 A2 WO2006041938 A2 WO 2006041938A2
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- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- B01J2219/00677—Ex-situ synthesis followed by deposition on the substrate
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- B01L2300/0829—Multi-well plates; Microtitration plates
Definitions
- the present invention relates to biochemical analysis and, more particularly, to a micropatterned plate with micro-pallets that facilitates addressable biochemical analysis.
- spotting by building cavities to collect the material (micro-wells), by treating the surface in specific regions, or by combinations of these methods. Most of these techniques do not work well for living cells. Once positioned, samples are almost never removed for further analysis or processing. Adherent cells are typically analyzed by plating them on a surface then looking for them using a microscope. The locations of the cells are random so that finding the cells can be a time consuming process. To speed this up, robotic systems that utilize machine vision are sometimes used to find the cells within the field of view of the microscope image. In some cases a subset of cells are isolated by the following method: A sacrificial base layer is placed over the plate. Cells are grown on the base layer.
- a high powered laser is used to cut a circle around the cells of interest, through the sacrificial layer.
- Cells can be isolated by peeling away the sacrificial layer, or by catapulting the cut material from plate using a high powered laser pulse, carrying the cell with it.
- Nonadherent cells can be analyzed quickly using a flow cytometer that rapidly flows a stream of cells past a detector apparatus.
- Cells of interest can be sorted by a downstream electrostatic system that moves droplets into collection containers. This method will also work for other biological media such as proteins and DNA if they can be attached to small beads. This method does not work well for larger samples (such as multi-celled organisms) and is difficult to multiplex.
- the present invention provides a plate manufactured in such a way that cells, micro ⁇ organisms, proteins, DNA, biomolecules and other biological media (herein called samples) can be positioned at specific locations (herein called sites) on the plate for the purpose of performing addressable analyses on the samples. Furthermore, in accordance with the present invention, some or all of the sites are built from a removable material (herein called pallets) so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis.
- the plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses.
- the plate can also contain structures that aid in the coupling between the plate and external instruments.
- the plate can also contain additional structures that aid in accessory operations, such as maintaining proper chemical conditions for the samples.
- the present invention advantageously provides (1) a plate with structures (sites) patterned on it that are intended to attach samples at known locations, (2) structures and plates that are treated or further patterned to improve the ability to perform analysis on the samples, (3) sites that are removable on demand so that laser cutting is not required, and released samples can be readily collected (4) additional micropatterned features such as structural elements, electrodes, and optical encoders that assist in the operation of the array plate, (5) placement of these sites in conventional cassettes or trays, and (6) placement of these sites in specialized cassettes or trays.
- the present invention enables high speed, addressable analysis of biological and chemical samples, as well as an efficient method for isolating subsets of samples from a larger population of samples. Further, objects and advantages of the invention will become apparent from the following detailed description.
- Figure IA is a micro-pallet plate having an array of micro-pallets.
- Figure IB is a side view of a micro-patterned plate with samples (cells) attached to pallets specific addressable sites.
- Figure 2 is a side view of another embodiment of a micro-patterned plate and illustrates a positive selection of a sample by releasing the pallet containing the sample from the plate.
- Figure 3 is a side view of another embodiment of a micro-patterned plate with samples (organisms) attached to specific addressable sites.
- Figure 4 is a side view of another embodiment of a micro-patterned plate with samples (cells) attached to specific addressable sites.
- Figure 5 is a side view of another embodiment of a micro-patterned plate placed at the bottom of a single well of a multiwell plate, allowing conventional tools to be used with the plate.
- Figure 6 is a side view of a plate showing the use of temporary or permanent dividers to allow samples of different types or histories to be plated on the plate at different locations or within different channels.
- Figures 7A and TB show steps in a process using a pallet plate for adherent cell screening and culturing.
- Figures 8A and 8B show steps in a process using a pallet plate for DNA screening.
- Figure 9 is a perspective view of an integrated pallet plate cassette for automated assays.
- Figures 1OA through M show steps in a process using an integrated pallet plate cassette for sample screening and culturing.
- Fig. 11 is a schematic of a high content screening and cell selection system utilizing a micro-pallet cassette comprising an array of micro-pallets.
- the present invention advantageously provides (1) a plate with structures (sites) patterned on it that are intended to attach samples at known locations, (2) structures and plates that can be treated or further pattered to improve the ability to perform analysis on the samples, (3) sites that are removable on demand so that laser cutting is not required, and wherein the released samples can be readily collected, (4) additional micropatterned features such as structural elements, electrodes, and optical encoders that assist in the operation of the array plate, (5) the placement of these sites in conventional cassettes or trays, and (6) the placement of these sites in specialized cassettes or trays.
- the invention enables high speed, addressable analysis of biological and chemical samples, as well as an efficient method for isolating subsets of samples from a larger population of samples.
- the system of the present invention advantageously provides removable regions where only one or a few samples can be attached. These regions are addressable, since their locations are known in advance. Optical encoders, electrodes, and the like enable this invention to be readily coupled to external instrumentation, enabling high speed addressable cell assays. Machines can move the plate to position any addressable site under the microscope. High magnification objectives can be used for imaging since only a single site is imaged (as opposed to a large field of many cells). For cells this enables much faster analysis than is currently available.
- the patterned system is placed within an existing tray or cassette, e.g., in a standard well-plate, then high throughput instruments can benefit from high speed cell analysis. Standard pipetting and handling equipment can be used.
- the system can be used with molecules, compounds, cells, organisms and biological and chemical media that adhere to the surfaces, as well as for samples that do not. Cavities or other entrapment devices can be used to position non-adherent samples.
- the system of the present invention also solves the problem of positive selection of samples.
- Removable pallets on an array allow one to quickly and selectively remove samples from the plate for further processing.
- the use of removable pallets eliminates the need to cut around the sample, greatly increasing the speed and throughput while reducing the complexity for selecting samples. Since the pallets are arranged on a plate, high speed analysis and sample selection can be performed at rates comparable to flow cytometry in a far simpler manner.
- the present invention fundamentally provides a plate 10 with an array of removable sites 13 (called pallets 12).
- the plate 10 or pallets 12 include modifications that further enhance the operation of the plate and pallets.
- the plate 10 is manufactured in such a way that cells, micro- organisms, proteins, DNA, biomolecules and other biological media (herein called samples) can be positioned at specific locations (herein called sites 13) on the plate 10 for the purpose of performing addressable analyses on the samples 14. Furthermore, some or all of the sites 13 are preferably built from a removable material (herein called pallets 12) so that a subset of the samples 14 of interest can be readily isolated from the plate 10 for further processing or analysis.
- the plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples 14, and that promote or assist in their analyses.
- the plate 10 can also contain structures that aid in the coupling between the plate 10 and external instruments.
- the plate 10 can also contain additional structures that aid in accessory operations, such as maintaining proper chemical conditions for the samples. Referring to Figure IB, the micro-patterned plate 1O 5 as depicted, includes samples
- cells 14 attached to specific addressable sites, i.e., the pallets 12.
- a microscope 16 of other detectors is used to image the samples 14 as the samples 14 are rapidly moved into position under the detector 16.
- Other detection schemes can be employed.
- the samples are attached to small, thin pallets 12 which adhere to the plate 10 at the sites 13.
- the use of pallets 12 is optional but beneficial for isolating single samples of interest.
- the pallet 12 can be removed at a later time to allow the experimenter to isolate samples of interest.
- the plate 10 has an array of sites 13 prepared on its surface having properties that preferably differ from the bulk material of the plate 10.
- the sites 13 are regions intended to be small enough to enable the entrapment of a few or single cells, micro- organisms, biomolecules or other biological or chemical media (herein called samples 14) at each site 13.
- the sites 13 can be constructed of a second material (herein called pallets 12) which can be removed from the supporting plate 10, carrying the sample 14 with it.
- the pallets 12 can be removed by a variety of mechanisms so that samples 14 can be isolated and removed from the plate 10 by removing their supporting pallet 12.
- the sites 13 can be prepared by locally modifying the surface chemistry or by physically altering the surface.
- the plate 10 allows for single samples 14, or small numbers of samples 14, to be collected at each site 13.
- Each site 13 can then be imaged, or probed with light or other energy (e.g., magnetic, electrical, mechanical, thermal energy) to determine the properties of the samples 14 trapped at the site 13 or to modify the sample 14 at the site 13.
- the sites 13 containing samples 14 of interest can be removed from the plate 10 for isolation from the plate 10 for further analysis or processing.
- the pallets 12 can also contain structures that assist in the movement or placement of the pallets 12 after removal from the plate 10.
- a pallet 12 can be removed by any means appropriate.
- Example methods include mechanically pushing or lifting the pallet 12 from the plate 10, using localized heat or light to change the adhesion property of the pallet 12, using acoustical or mechanical shock to dislodge the pallet 12 from the plate 10, using high energy laser pulses to dislodge the pallet 12 from the plate 10, changing the electrical or magnetic properties of the pallet 12, and the like.
- FIG 2 an example of pallet removal using a laser pulse 17 from a laser 18 is shown.
- a positive selection of a sample 14 is accomplished by releasing the pallet 12 containing the sample 14 from the plate 10.
- other methods of pallet release can be employed including the application of mechanical, electrical, thermal, optical, magnetic energy.
- the released pallet 12 can be flowed downstream for collection, or can be collected by other means (such as decanting or pipetting).
- the sites 13 are preferably formed close together so that the plate 10 can be moved under an analysis instrument to rapidly perform analysis of many sites 13. For example, if the sites 13 are positioned 0.1 mm apart, then the plate 10 can be moved at 50 mm/sec to analyze 500 samples per second. Samples 14 can be attached to the sites 13 in any of a various number of methods. For example, living cells can be allowed to float in a medium until they attach to the sites. The remaining cells can be washed away leaving an addressable array of cells that can be rapidly imaged. Conventional methods such as spotting, silkscreening, stenciling, lithography, optical manipulation, or mechanical attachment can also be used to attach the samples to the sites.
- the sites 13 can form rectangular or other regular patterns (e.g., hexagonal, circular, linear, etc.), or can be randomly oriented.
- the patterned sites can be positioned within a larger structure such as at the bottom of a multi-well plate.
- the patterned plate can allow other structures to be placed within it to facilitate other functions, for example the use of temporary dividers that allow different samples to be introduced into different regions of the plate, or fluidic structures (e.g., channels) to facilitate the flow of buffer across the sites (as illustrated in Figure 3).
- a micro-patterned plate 20 is shown with samples 24 (organisms) attached to specific addressable sites 23.
- samples 24 organs
- a 3-D structured pattern 25 on the plate 20 assists in the collection of the sample 24 at the specific sites, where they can be attached directly to the plate 20 or to small pallets 22 at each site 23.
- the physical shape of the surface can be modified to enhance the capture at sites (and not at non-sites), or to improve the analysis.
- the sites can be formed on top of posts. This provides the advantage that non-sites are out of focus (see 35,
- Figure 4 for a microscopy imaging system, reducing background in the image.
- Other examples can include cavities that trap samples within them, or opaque regions on the plate.
- optical encoders, electrodes, or magnetic devices can be included on the plate to facilitate placement; sensors can be used to test for growth conditions, fiducial marks can be included for optical alignment, etc.
- a micro-patterned plate 30 includes samples (cells) 34 attached to pallets 32 or posts at specific addressable sites 33.
- a microscope objective 36 is used to image the "in focus" samples 34 as they are rapidly moved into position under the objective 36.
- Other included features include patterned electrodes 37, patterned opaque regions 38, and externally applied electrical fields 39 that can be used to lyse specific cells of interest.
- the chemical property of the sites can also be modified to enhance the capture at the sites (and not at non-sites), or to improve the analysis.
- surface chemistry can be modified to make some regions hydrophobic and other hydrophilic to enhance cell adhesion at the hydrophobic sites.
- Surface chemistry can also be used to make a non-site of the plate opaque and site-regions transparent to provide local apertures for enhanced optical imaging.
- the array of sites can be produced within existing industry standard trays and cassettes.
- the sites can be fabricated within the bottoms of multi-well plates, providing high speed addressable assays to industry standard equipment (see, e.g., Figure 5).
- the array of sites can also be produced within a customized system of cartridges. An example of a customized cartridge is shown in Figure 6.
- a micro-patterned plate 40 is placed at the bottom of a single well 47 of a multiwell plate 41, allowing conventional tools to be used with the plate 40.
- the micropatterned plate 40 includes a plurality of pallets 42 forming a plurality of sites 43 with samples 44.
- a buffer solution fills the single well.
- a micro-patterned plate 50 is shown to include temporary or permanent dividers 51 attached to a fluidic cap 55 to allow samples 54 of different types or histories to be plated on the plate 50 at different locations. This allows multiplexed analysis to be done on a single plate.
- the dividing structures 51 can also facilitate the flow of buffers over the sample regions for extraction of released pallets 52.
- FIGS 7A and 7B steps in a process using a pallet plate for adherent cell screening and culturing are shown.
- This example illustrates how the disclosed system can be used to screen for rare cells from a large collection of cells.
- the adherent cells can be taken from a patient biopsy and the disclosed system can be used to search for and select cells that show unusual or malignant behavior.
- adherent cells might be treated with a DNA vector in hopes of transfecting the cells, and the system used to find and isolate the cells that were properly transfected.
- cells 60 are pretreated, at step 1, according to an appropriate protocol, the cells 60 are then dispersed, at step 2, over the plate 70 and allowed to attach to the plate 70 or the pallet 72 at a plurality of sites 73.
- This can be done in a multi- well plate 62, as shown, or a single well plate.
- the cells adhere, as a sample 74, at step 3, to the plate 70 or pallet 72. Since the plate is treated and patterned, cells prefer to adhere at specific sites.
- the plate is then preferably washed and further assay work is preferably performed to label the cells of interest.
- the plate is screened by detector 76, at step 5, to gain statistical information about the cell population and to identify cells of interest.
- Pallets 72a containing the cells of interest are (sample 74) dislodged (released), at step 6, from the plate, preferably, e.g., by a high energy laser pulse 77 from a laser 78.
- the free floating pallets 72a are then collected, at step 7, from the buffer solution.
- new cell cultures are grown from the released cells 74.
- FIG. 8A and 8B steps in a process using a pallet plate for DNA screening are shown.
- This example illustrates how the disclosed system can be used to screen for rare DNA strands from a large collection of DNA.
- an unknown disease causing agent can be screened against a DNA plate to select strands of interest. Then the strands of interest can be isolated and PCR performed to amplify them for further analysis.
- the steps of the process are as follows: At step 1, a plate 80 is spotted with oligonucleotides at specific sites 83 which act as targets for DNA strands. The oligos are also prepared to act as controls.
- DNA 85 is taken from sample, denatured and pretreated according to an appropriate protocol.
- DNA 85 is dispersed over the plate 80 and allowed to hybridize to their matching targets at specific sites 83.
- the plate is thoroughly washed to remove unbound DNA. Further assay work is performed to label the DNA of interest.
- the plate is then screened by the detector 86, at step 5, for statistical analysis of the sample and to identify DNA of interest.
- the pallets 82a containing the DNA of interest 84 are dislodged (released), at step 6, from the plate 80 by a high energy laser pulse 87 from a laser 88.
- the free floating pallets are collected from the buffer solution.
- DNA 84 is denatured from the pallet and used in PCR reaction to amplify the sample.
- an integrated pallet plate cassette 90 for automated assays is illustrated. This example illustrates how the disclosed system can be integrated into other systems to produce an automated cartridge system.
- the integrated pallet plate cassette 90 includes a micropallet plate 99 with a plurality of pallets 92 formed in three arrays on the plate 99, and a fluidic cap 91 with small channels 95 formed on its underside. The cap 91 mates with the micropallet plate 99 to flow buffers over the pallets 92.
- Figures 1OA through M a process using a micro-machined integrated pallet plate cassette 100 is shown.
- the cassette 100 includes a pallet plate 109 that preferably includes a pre-set array of releasable pallets 102 for cell culturing that are releasably positioned atop of the plate 109 formed of glass or the like.
- the pallets 102 are preferably treated to promote cell growth at the center of the pallets 102.
- the pallets 102 are preferably indexed, e.g., bar coded, so that their positions are known in advance of use of the cassette 100.
- the cap 101 is closed on to the plate 109 revealing an access hole 107.
- cells are dispersed over the plate 109 and allowed to attach to the plate at specific sites 10.
- the plate 109 is then screened by the detector 106, as depicted in Figure 1OE, for statistical analysis of the sample and to identify cells of interest.
- a pallet 102a containing the cells of interest is dislodged (released), as shown in Figure 1OF, from the plate 109 by a high energy laser pulse from a laser 108.
- the free floating pallet 102a is collected from the buffer solution toward the end of the plate 109.
- a second pallet 102b containing additional cells of interest is dislodged (released) from the plate 109 by a high energy laser pulse from a laser 108.
- the free floating pallet 102b is collected from the buffer solution toward the end of the plate 109.
- the pallets 102a and 102b are extracted through access hole 107 using an extractor 110.
- New cell cultures are grown from the released cells, as shown in Figures 1OL and 1OM.
- a cassette 170 comprising a substrate or plate 179 formed of glass or the like and a cap 171.
- the plate 169 can include an array of micro-pallets 172 - e.g., providing 500,000 (50 x 50 microns) pallet sites — positioned on the plate 179.
- the cassette 170 can be used with a microscope attachment 150 for imaging, fluorescent analysis, sorting, and the like. Analysis software provided on a computer 160 can be used for high content screening and cell selection.
- a pallet extractor can be used to extract a selected pallet from the cassette 170.
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- Chemical Kinetics & Catalysis (AREA)
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- Biochemistry (AREA)
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- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MX2007004068A MX2007004068A (en) | 2004-10-04 | 2005-10-04 | Micropatterned plate with micro-pallets for addressable biochemical analysis. |
EP05802866A EP1799864A4 (en) | 2004-10-04 | 2005-10-04 | Micropatterned plate with micro-pallets for addressable biochemical analysis |
JP2007534910A JP4887298B2 (en) | 2004-10-04 | 2005-10-04 | Micropatterned plates with micropallets for addressable biochemical analysis |
AU2005294377A AU2005294377B2 (en) | 2004-10-04 | 2005-10-04 | Micropatterned plate with micro-pallets for addressable biochemical analysis |
CA2580853A CA2580853C (en) | 2004-10-04 | 2005-10-04 | Micropatterned plate with micro-pallets for addressable biochemical analysis |
IL182039A IL182039A0 (en) | 2004-10-04 | 2007-03-20 | Micropatterned plate with micro-pallets for addressable biochemical analysis |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US61588204P | 2004-10-04 | 2004-10-04 | |
US60/615,882 | 2004-10-04 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2006041938A2 true WO2006041938A2 (en) | 2006-04-20 |
WO2006041938A3 WO2006041938A3 (en) | 2007-04-12 |
Family
ID=36148879
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2005/035860 WO2006041938A2 (en) | 2004-10-04 | 2005-10-04 | Micropatterned plate with micro-pallets for addressable biochemical analysis |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP1799864A4 (en) |
JP (1) | JP4887298B2 (en) |
KR (1) | KR20070062578A (en) |
CN (1) | CN101035910A (en) |
AU (1) | AU2005294377B2 (en) |
CA (1) | CA2580853C (en) |
IL (1) | IL182039A0 (en) |
MX (1) | MX2007004068A (en) |
RU (1) | RU2007116801A (en) |
WO (1) | WO2006041938A2 (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007127990A2 (en) * | 2006-04-28 | 2007-11-08 | The Regents Of The University Of California | Method of manufacture of a plate of releasable elements and its assembly into a cassette |
US7695954B2 (en) | 2004-10-04 | 2010-04-13 | The Regents Of The University Of California | Micropatterned plate with micro-pallets for addressable biochemical analysis |
US7759119B2 (en) | 2006-04-10 | 2010-07-20 | The Regents Of The University Of California | Systems and methods for efficient collection of single cells and colonies of cells and fast generation of stable transfectants |
US7951580B2 (en) | 2004-04-21 | 2011-05-31 | The Regents Of The University Of California | Automated, programmable, high throughput, multiplexed assay system for cellular and biological assays |
US8748085B2 (en) | 2007-07-25 | 2014-06-10 | The Regents Of The University Of California | Use of photosensitized Epon epoxy resin 1002F for MEMS and bioMEMS applications |
EP2919008A4 (en) * | 2012-12-07 | 2016-06-29 | Snu R&Db Foundation | Method for isolating biochemical molecules on microarray substrate |
US9487745B2 (en) | 2007-07-27 | 2016-11-08 | The Regents Of The University Of California | Micro-patterned plate composed of an array of releasable elements surrounded with solid or gel walls |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101179553B1 (en) | 2008-12-22 | 2012-09-05 | 한국전자통신연구원 | Compartment unit for cell culture and array having the same |
CN102943027B (en) * | 2012-11-22 | 2014-11-26 | 清华大学 | Positive pressure type high-throughput single-celled patterning device |
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WO2003039750A1 (en) | 2001-11-07 | 2003-05-15 | Genvault Corporation | Apparatus, system, and method of archival and retrieval of samples |
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US6875620B1 (en) * | 1996-10-31 | 2005-04-05 | Agilent Technologies, Inc. | Tiling process for constructing a chemical array |
JP3432173B2 (en) * | 1999-05-12 | 2003-08-04 | アロカ株式会社 | Biological tissue processing equipment |
US6770441B2 (en) * | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
JP4755368B2 (en) * | 2001-07-24 | 2011-08-24 | 株式会社セルシード | High-density cell array substrate, manufacturing method, and use thereof |
US6632660B2 (en) * | 2002-01-04 | 2003-10-14 | Applera Corporation | Petal-array support for use with microplates |
JP4167431B2 (en) * | 2002-02-26 | 2008-10-15 | 株式会社Dnaチップ研究所 | Inspection board for biochemical inspection |
EP1575707A1 (en) * | 2002-09-12 | 2005-09-21 | Cyvera Corporation | Method and apparatus for aligning elongated microbeads in order to interrogate the same |
-
2005
- 2005-10-04 EP EP05802866A patent/EP1799864A4/en not_active Ceased
- 2005-10-04 MX MX2007004068A patent/MX2007004068A/en unknown
- 2005-10-04 CA CA2580853A patent/CA2580853C/en not_active Expired - Fee Related
- 2005-10-04 AU AU2005294377A patent/AU2005294377B2/en not_active Ceased
- 2005-10-04 WO PCT/US2005/035860 patent/WO2006041938A2/en active Application Filing
- 2005-10-04 CN CNA2005800336952A patent/CN101035910A/en active Pending
- 2005-10-04 JP JP2007534910A patent/JP4887298B2/en not_active Expired - Fee Related
- 2005-10-04 KR KR1020077009234A patent/KR20070062578A/en not_active Application Discontinuation
- 2005-10-04 RU RU2007116801/13A patent/RU2007116801A/en not_active Application Discontinuation
-
2007
- 2007-03-20 IL IL182039A patent/IL182039A0/en unknown
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US5998129A (en) | 1996-02-05 | 1999-12-07 | P.A.L.M. Gmbh | Method and device for the contactless laser-assisted microinjection, sorting and production of biological objects generated in a planar manner |
US20030059764A1 (en) | 2000-10-18 | 2003-03-27 | Ilya Ravkin | Multiplexed cell analysis system |
WO2003039750A1 (en) | 2001-11-07 | 2003-05-15 | Genvault Corporation | Apparatus, system, and method of archival and retrieval of samples |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7951580B2 (en) | 2004-04-21 | 2011-05-31 | The Regents Of The University Of California | Automated, programmable, high throughput, multiplexed assay system for cellular and biological assays |
US7695954B2 (en) | 2004-10-04 | 2010-04-13 | The Regents Of The University Of California | Micropatterned plate with micro-pallets for addressable biochemical analysis |
US7759119B2 (en) | 2006-04-10 | 2010-07-20 | The Regents Of The University Of California | Systems and methods for efficient collection of single cells and colonies of cells and fast generation of stable transfectants |
WO2007127990A2 (en) * | 2006-04-28 | 2007-11-08 | The Regents Of The University Of California | Method of manufacture of a plate of releasable elements and its assembly into a cassette |
WO2007127990A3 (en) * | 2006-04-28 | 2009-04-23 | Univ California | Method of manufacture of a plate of releasable elements and its assembly into a cassette |
US8748085B2 (en) | 2007-07-25 | 2014-06-10 | The Regents Of The University Of California | Use of photosensitized Epon epoxy resin 1002F for MEMS and bioMEMS applications |
US9487745B2 (en) | 2007-07-27 | 2016-11-08 | The Regents Of The University Of California | Micro-patterned plate composed of an array of releasable elements surrounded with solid or gel walls |
EP2919008A4 (en) * | 2012-12-07 | 2016-06-29 | Snu R&Db Foundation | Method for isolating biochemical molecules on microarray substrate |
US10883136B2 (en) | 2012-12-07 | 2021-01-05 | Snu R&Db Foundation | Method of isolating biochemical molecules on microarray substrate |
Also Published As
Publication number | Publication date |
---|---|
IL182039A0 (en) | 2007-07-24 |
JP4887298B2 (en) | 2012-02-29 |
AU2005294377A1 (en) | 2006-04-20 |
EP1799864A2 (en) | 2007-06-27 |
AU2005294377B2 (en) | 2011-12-01 |
CN101035910A (en) | 2007-09-12 |
KR20070062578A (en) | 2007-06-15 |
JP2008516199A (en) | 2008-05-15 |
CA2580853C (en) | 2014-04-29 |
MX2007004068A (en) | 2007-06-04 |
CA2580853A1 (en) | 2006-04-20 |
EP1799864A4 (en) | 2009-06-03 |
WO2006041938A3 (en) | 2007-04-12 |
RU2007116801A (en) | 2008-11-10 |
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