WO2006036952A3 - Monitoring of cell adhesion and spreading - Google Patents

Monitoring of cell adhesion and spreading Download PDF

Info

Publication number
WO2006036952A3
WO2006036952A3 PCT/US2005/034561 US2005034561W WO2006036952A3 WO 2006036952 A3 WO2006036952 A3 WO 2006036952A3 US 2005034561 W US2005034561 W US 2005034561W WO 2006036952 A3 WO2006036952 A3 WO 2006036952A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
spreading
cell adhesion
impedance
monitoring
Prior art date
Application number
PCT/US2005/034561
Other languages
French (fr)
Other versions
WO2006036952A2 (en
Inventor
Yama A Abassi
Josephine Atienza
Xiao Xu
Xiaobo Wang
Original Assignee
Yama A Abassi
Josephine Atienza
Xiao Xu
Xiaobo Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2004/037696 external-priority patent/WO2005047482A2/en
Priority claimed from US11/055,639 external-priority patent/US7560269B2/en
Priority claimed from PCT/US2005/004481 external-priority patent/WO2005077104A2/en
Priority claimed from US11/198,831 external-priority patent/US8263375B2/en
Priority claimed from US11/197,994 external-priority patent/US7468255B2/en
Priority claimed from PCT/US2005/027943 external-priority patent/WO2006017762A2/en
Priority to CA002580548A priority Critical patent/CA2580548A1/en
Priority to EP05812268A priority patent/EP1800312A4/en
Application filed by Yama A Abassi, Josephine Atienza, Xiao Xu, Xiaobo Wang filed Critical Yama A Abassi
Priority to PCT/US2005/042684 priority patent/WO2006058185A2/en
Priority to EP05852157.6A priority patent/EP1815025B1/en
Priority to US11/286,882 priority patent/US8206903B2/en
Publication of WO2006036952A2 publication Critical patent/WO2006036952A2/en
Publication of WO2006036952A3 publication Critical patent/WO2006036952A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
    • G01N29/02—Analysing fluids
    • G01N29/036—Analysing fluids by measuring frequency or resonance of acoustic waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2291/00—Indexing codes associated with group G01N29/00
    • G01N2291/02—Indexing codes associated with the analysed material
    • G01N2291/025—Change of phase or condition
    • G01N2291/0256—Adsorption, desorption, surface mass change, e.g. on biosensors
    • G01N2291/0257—Adsorption, desorption, surface mass change, e.g. on biosensors with a layer containing at least one organic compound

Abstract

The present invention includes devices and methods for dynamically monitoring cell adhesion and cell spreading. Cells are added to a microelectronic cell sensor array operably connected to an impedance analyzer. The device also includes a coating including biological molecule or organic compound capable of interacting with the cell. Cell adhesion and cell mobility is determined by detecting changes in impedance and comparing impedance or cell index values between samples.
PCT/US2005/034561 2002-12-20 2005-09-27 Monitoring of cell adhesion and spreading WO2006036952A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002580548A CA2580548A1 (en) 2004-09-27 2005-09-27 Monitoring of cell adhesion and spreading
EP05812268A EP1800312A4 (en) 2004-09-27 2005-09-27 Dynamic monitoring of cell adhesion and spreading using the rt-ces system
PCT/US2005/042684 WO2006058185A2 (en) 2004-11-24 2005-11-23 Device and method for electroporation-based delivery of moecules into cells and dynamic monitoring of cell responses
US11/286,882 US8206903B2 (en) 2002-12-20 2005-11-23 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
EP05852157.6A EP1815025B1 (en) 2004-11-24 2005-11-23 Dynamic monitoring of cell responses to the delivery of molecules into the cell

Applications Claiming Priority (46)

Application Number Priority Date Filing Date Title
US61387204P 2004-09-27 2004-09-27
US61374904P 2004-09-27 2004-09-27
US60/613,749 2004-09-27
US60/613,872 2004-09-27
US61460104P 2004-09-29 2004-09-29
US60/614,601 2004-09-29
PCT/US2004/037696 WO2005047482A2 (en) 2003-11-12 2004-11-12 Real time electronic cell sensing systems and applications for cell-based assays
USPCT/US2004/037696 2004-11-12
US10/987,732 2004-11-12
US10/987,732 US7192752B2 (en) 2002-12-20 2004-11-12 Real time electronic cell sensing systems and applications for cell-based assays
US63007104P 2004-11-22 2004-11-22
US63013104P 2004-11-22 2004-11-22
US60/630,071 2004-11-22
US60/630,131 2004-11-22
US63080904P 2004-11-24 2004-11-24
US60/630,809 2004-11-24
US63301904P 2004-12-03 2004-12-03
US60/633,019 2004-12-03
US64715905P 2005-01-26 2005-01-26
US64707505P 2005-01-26 2005-01-26
US64718905P 2005-01-26 2005-01-26
US60/647,189 2005-01-26
US60/647,075 2005-01-26
US60/647,159 2005-01-26
US11/055,639 US7560269B2 (en) 2002-12-20 2005-02-09 Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
US11/055,639 2005-02-09
USPCT/US2005/004481 2005-02-09
PCT/US2005/004481 WO2005077104A2 (en) 2004-02-09 2005-02-09 Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
US65390405P 2005-02-17 2005-02-17
US60/653,904 2005-02-17
US66089805P 2005-03-10 2005-03-10
US66082905P 2005-03-10 2005-03-10
US60/660,898 2005-03-10
US60/660,829 2005-03-10
US67367805P 2005-04-21 2005-04-21
US60/673,678 2005-04-21
US68942205P 2005-06-10 2005-06-10
US60/689,422 2005-06-10
PCT/US2005/027943 WO2006017762A2 (en) 2004-08-04 2005-08-04 Dynamic monitoring of activation of g-protein (gpcr) and receptor tyrosine kinase (rtk) in living cells using real-time microelectronic cell sensing technology
USPCT/US2005/027891 2005-08-04
US11/197,994 2005-08-04
US11/197,994 US7468255B2 (en) 2002-12-20 2005-08-04 Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology
USPCT/US2005/027943 2005-08-04
PCT/US2005/027891 WO2006015387A2 (en) 2004-08-04 2005-08-04 Method for assaying cytolytic activity of target cells
US11/198,831 US8263375B2 (en) 2002-12-20 2005-08-04 Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US11/198,831 2005-08-04

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US11/235,938 Continuation-In-Part US7732127B2 (en) 2002-07-20 2005-09-27 Dynamic monitoring of cell adhesion and spreading using the RT-CES system

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/286,882 Continuation-In-Part US8206903B2 (en) 2002-07-20 2005-11-23 Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses

Publications (2)

Publication Number Publication Date
WO2006036952A2 WO2006036952A2 (en) 2006-04-06
WO2006036952A3 true WO2006036952A3 (en) 2006-12-21

Family

ID=56290735

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/034561 WO2006036952A2 (en) 2002-12-20 2005-09-27 Monitoring of cell adhesion and spreading

Country Status (3)

Country Link
EP (1) EP1800312A4 (en)
CA (1) CA2580548A1 (en)
WO (1) WO2006036952A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2199783A1 (en) * 2008-12-17 2010-06-23 Koninklijke Philips Electronics N.V. Microelectronic device for measuring cell adhesion
CN111172024A (en) * 2019-12-31 2020-05-19 福建农林大学 Device for in-situ characterization of inter-species direct electron transfer

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6051422A (en) * 1997-05-30 2000-04-18 Board Of Trustees, Leland Stanford, Jr., University Hybrid biosensors
US6132683A (en) * 1998-12-23 2000-10-17 Matsushita Electric Industrial Co., Ltd. Cell potential measuring electrode and measuring apparatus using the same
US20030157587A1 (en) * 2000-04-17 2003-08-21 Rafael Gomez Biosensor and related method
US20030166015A1 (en) * 1999-04-15 2003-09-04 Zarowitz Michael A. Multiplexed analysis of cell-substrate interactions
US20040146849A1 (en) * 2002-01-24 2004-07-29 Mingxian Huang Biochips including ion transport detecting structures and methods of use

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100577811C (en) * 2002-07-20 2010-01-06 美国艾森生物科学公司 Apparatuses and method based on impedance for analyzing cells and granules

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6051422A (en) * 1997-05-30 2000-04-18 Board Of Trustees, Leland Stanford, Jr., University Hybrid biosensors
US6132683A (en) * 1998-12-23 2000-10-17 Matsushita Electric Industrial Co., Ltd. Cell potential measuring electrode and measuring apparatus using the same
US20030166015A1 (en) * 1999-04-15 2003-09-04 Zarowitz Michael A. Multiplexed analysis of cell-substrate interactions
US20030157587A1 (en) * 2000-04-17 2003-08-21 Rafael Gomez Biosensor and related method
US20040146849A1 (en) * 2002-01-24 2004-07-29 Mingxian Huang Biochips including ion transport detecting structures and methods of use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1800312A4 *

Also Published As

Publication number Publication date
WO2006036952A2 (en) 2006-04-06
CA2580548A1 (en) 2006-04-06
EP1800312A2 (en) 2007-06-27
EP1800312A4 (en) 2011-10-05

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