WO2006035808A1

WO2006035808A1 - Parasite control composition for beehive and method of control therewith

Info

Publication number: WO2006035808A1
Application number: PCT/JP2005/017826
Authority: WO
Inventors: Atsushi Sakai; Yoshiaki Higashino; Hiroshi Kodama
Original assignee: Nihon Nohyaku Co., Ltd.
Priority date: 2004-09-28
Filing date: 2005-09-28
Publication date: 2006-04-06

Abstract

A parasite control agent composition for beehive, comprising fenpyroximate as an active ingredient. There is provided a parasite control agent composition for beehive that would not cause any harmful effect on honeybees and would exert an excellent control effect on parasites being resistant to current active ingredients. Further, there is provided a method of using the parasite control agent composition.

Description

Specification

Parasite control composition and control method in bee population

Technical field

[0001] The present invention relates to a parasite control agent composition and control method in a bee population containing fenpyroximate as an active ingredient.

Background art

[0002] Parasite damage in honeybee populations is a serious problem for beekeepers, especially because damage caused by honeybees (Varroa destructor) is fatal to honeybee populations. I came. For example, it is known that synthetic pyrethroids such as fulvalinate and organic phosphorus agents such as coumaphos can be used as parasite control agents in the bee population. In addition, the method of contacting the bees and the method of installing them in the nest box has been devised (for example, see Patent Documents 1 to 4). On the other hand, fenpyroximate is a known compound known to be useful as an insecticide / acaricide for agriculture and horticulture (see, for example, Patent Document 5 and Non-Patent Document 1).

Patent Document 1: Japanese Patent Laid-Open No. 61_1609

Patent Document 2: Japanese Patent Laid-Open No. 62-108803

Patent Document 3: Japanese Patent Laid-Open No. 5_78206

Patent Document 4: Japanese Translation of Special Publication 2003-500424

Patent Document 5: Japanese Patent Laid-Open No. 63-183564

Non-Patent Document 1: The Pestic side MANYUANORE, 11th edition, pages 531-532 (The Pestic iae Manual, Eleventh Edition, p. 531-532)

Disclosure of the invention

Problems to be solved by the invention

[0003] However, as a result of minimizing the appearance of resistant parasites and adverse effects on bees against existing active ingredients, there are cases where sufficient control cannot be achieved, which is more effective. Parasitic control agents and control methods have been sought. Means for solving the problem

[0004] In order to solve the above problems, the present inventors have conducted intensive studies. As a result, by using fenpyroximate, which is a known compound, as an active ingredient, parasites in a bee population that do not adversely affect bees are used. In addition, the present inventors have found that a high control effect can be obtained, and further, by mixing an existing active ingredient with fenpyroximate, a parasite control composition having a higher effect has been found, and the present invention has been completed.

That is, the present invention

(1) a parasite control agent composition in a bee population comprising fenpyroximate as an active ingredient;

(2) The parasite control composition according to (1), further comprising one or more compounds selected from fulvalinate, acrinatrin, coumaphos or amitraz as an active ingredient,

(3) The parasite control composition according to (1) or (2), wherein the parasite in the bee population is a honey bee mite (Varroa destructor),

(4) An effective amount of an active ingredient is brought into contact with a bee; (1) to (3) a method for using the parasiticide control composition according to (1) to (3) above, and

(5) The present invention relates to a method for using the parasiticidal composition according to any one of (1) to (3), wherein an effective amount of an active ingredient is mixed in water or feed.

The invention's effect

[0005] The present invention relates to a composition for controlling a parasite in a bee population that exhibits an excellent control effect against a parasitic insect having resistance to an existing active ingredient that does not adversely affect the bee and its It provides usage.

BEST MODE FOR CARRYING OUT THE INVENTION

[0006] In the present invention, "honeybee" refers to the whole of a bee population, that is, adult (male bee, worker bee), larva, egg, pupa and queen bee. The types of honey bees include Apis mellifera, Apis cerana, Sano honey bees (Apis koschevn ikovi), Apis nuluensis, Black honey bees (Apis nigrocin) ), Apis dorsata, Apis laboriosa, Apis florea, Apis andreniformis, and the like. "three-leaved Parasites in a bee population refer to a parasite that parasitizes a bee population or nest box and adversely affects the survival of the bees. Specifically, the honeybees are Varroa destru ctor, Varroa underwoodi. Alternatively, parasitic mites such as A carapis woodi can be mentioned. Above all, the honey bee mite (Varroa destructor) is particularly important as a control target because it absorbs the body fluid of honey bees, weakens honey bees and causes death, and mediates pathogenic viruses.

The active ingredients to control these parasites are not only effective against parasites, but also have an adverse effect on bees (eg, decreased activity, increased mortality, decreased honey collection) No compound is required. Although many insecticides and acaricides are known, the active ingredients that can actually be used for “parasites in the bee population” are limited to some compounds from the above conditions, and fluvalinate, acrinatrin, coumaphos, amitraz Is only used. Tebufenpyrad, which has a mechanism of action similar to that of fenpyroximate (complex I inhibition of mitochondrial electron transport), has been reported to be unusable for this application due to its high toxicity to bees (eg, J. Econ Entomol., 96 ( 2), p. 259-263 (2003)). Under these circumstances, fenpyroximate was found to be a compound that satisfies the above requirements.

[0007] The method for using the parasiticide control composition of the present invention includes, for example, direct application to bees or nest boxes, fumigation, and support or application of active ingredients on a carrier such as a plastic material in the nest box. The active ingredient may be directly brought into contact with the bee, the active ingredient may be mixed in water or feed, and the active ingredient may be infiltrated into the entire bee population through grooming. When the active ingredient is brought into direct contact by spraying or smoking, it can be formulated into a convenient shape according to the technique used in general agricultural chemical formulations. In other words, the active ingredient is mixed with an appropriate inert carrier or, if necessary, with an auxiliary agent in an appropriate ratio and dissolved, separated, suspended, mixed, impregnated, adsorbed or adhered, and an appropriate agent. It may be used in the form of a suspension, milk suspension, emulsion, liquid, wettable powder, granule, powder, tablet or the like.

[0008] The inert carrier that can be used in the present invention may be either solid or liquid. Examples of materials that can be used as a solid carrier include vegetable powders (for example, soybean flour, cereal grains). Powder, wood powder, bark powder, saw powder, tobacco stem powder, tarmi shell powder, bran, fiber powder, residues after extraction of plant extracts, etc.), synthetic polymers such as pulverized synthetic resin, clays (for example, kaolin, Bentonite, acid clay, etc.), talc (eg, talc, pyrophyllite, etc.), silica (eg, diatomaceous earth, silica sand, mica, white carbon (synthetic highly dispersed silicic acid, also called hydrous finely divided silicon, hydrous silicic acid) Some contain calcium as the main component.)}, Activated charcoal, natural minerals (eg, powder, pumice, attapulgite, zeolite) , Polyethylene, polypropylene, polyvinylidene chloride, etc.), inorganic mineral powders such as calcium carbonate, calcium phosphate, ammonium sulfate, phosphorous acid Ammonium nitrate, urea, fertilizer salts depreciation etc., can be exemplified compost or the like, which are used alone or in the form of a mixture of two or more thereof.

[0009] The material that can be used as a liquid carrier is selected from those having solvent ability itself and those capable of dispersing an active ingredient compound with the aid of an auxiliary agent without having solvent ability. For example, water, alcohols (eg, methanol, ethanol, isopropanol, butanol, ethylene glycol, etc.), ketones (eg, acetone, methyl ethyl ketone, methyl isobutyl ketone, diisoptyl ketone, cyclohexanone, etc.), ethers (For example, ethyl ether, dioxane, cellosolve, dipropyl ether, tetrahydrofuran, etc.), aliphatic hydrocarbons (for example, kerosene, mineral oil, etc.), aromatic hydrocarbons (for example, benzene, toluene, xylene, solvent naphtha, Alkyl naphthalene, etc.), esters (for example, acetic acid) Ethyl, diisopropylpropyl phthalate, dibutyl phthalate, dioctyl phthalate, etc.), amides (eg, dimethylformamide, jetylformamide, dimethylacetamide, etc.), nitriles (eg, acetonitrile, etc.), dimethyl sulfoxides, etc. These can be used alone or in the form of a mixture of two or more.

[0010] As other adjuvants, the following representative adjuvants can be mentioned, and these adjuvants are used depending on the purpose, and in some cases, two or more kinds of adjuvants are used. It can be used in combination, or in some cases, no adjuvants can be used at all. Surfactants can be used for emulsification, dispersion, solubilization and Z or wetting purposes of the active compound, for example, polyoxyethylene alkyl ether, polyoxyethylene alkyl aryl ether, poly Surfactants such as oxyethylene higher fatty acid ester, polyoxyethylene resin acid ester, polyalkylaryl sulfonate, naphthalene sulfonic acid condensate, lignin sulfonate and higher alcohol sulfate can be mentioned. For the purpose of stabilizing the dispersion, sticking and / or binding of the active compound, for example, casein, gelatin, starch, methylcellulose, carboxymethylcellulose, gum arabic, polyvinyl alcohol, pine oil, coconut oil, bentonite, lignin sulfonic acid Adjuvants such as salt can be used. In order to improve the fluidity of solid products, for example, auxiliary agents such as waxes, stearates and phosphoric acid alkyl esters can be used. As a peptizer for a suspension product, for example, auxiliaries such as naphthalene sulfonic acid condensate and condensed phosphate can be used. As the antifoaming agent, for example, auxiliary agents such as silicone oil can be used.

The preparation thus obtained may be sprayed directly or diluted with water as necessary and sprayed onto a bee or a hive. In addition, for smoke agents, it is acceptable if smoke treatment is applied to bees or beehives according to conventional methods.

[0011] When a carrier such as a plastic material, on which an active ingredient is carried or applied, is installed in a nest box, examples of the carrier that can be used include polysalt bulbule (PVC), polyvinylidene chloride, polyethylene, Various polymers such as polypropylene, polyurethane, polyvinyl polypyrrolidone (PVPP), polyester, polyimide, polyamide, etc., wood chips, wood chips, paper, etc. can be mentioned. The active ingredients in these carriers are diluted with a solvent, etc. if necessary. Can be supported or applied, processed into an appropriate shape, and placed in the nest box. Further, it can be kneaded into a plastic carrier by a conventional method using a plasticizer such as citrate esters such as triethyl citrate, dioctyl phthalate, and phthalates such as jetyl hexyl phthalate. When the bee comes into contact with the carrier containing the active ingredient, the active ingredient is attached to the honeybee worm body, and further, the active ingredient is diffused to other bees by the activity within the bee population. Parasite control is possible.

[0012] In the parasite control composition of the present invention, the dosage of fenpyroximate, which is an active ingredient, is not constant depending on the form of use, but it may be appropriately selected within the range of 0.1 ppm to 100 ppm when sprayed directly. It is preferably 1 ppm to 50 ppm. Plastic material When the active ingredient is supported on or applied to a carrier such as the above, the amount of the active ingredient may be appropriately selected within the range of 1 part by weight to 20 parts by weight with respect to 100 parts by weight of the parasite control agent composition. ~ 10 parts by weight.

[0013] When an active ingredient is mixed into water or a feed and the active ingredient is infiltrated into all the bee population through grooming or the like, the feed is a sugar granule or a mixture, solution, suspension or emulsion containing sugar. If the active ingredient of 0.0001% to 0.01% by weight, preferably 0.0005% to 0.005% by weight, is diluted with a solvent or the like as necessary, it is added to these. good. Further, a feed solution adjusted to a high concentration can be diluted with water or a sugar solution so as to be within the concentration range of the active ingredient.

[0014] Also, by using fenpyroximate in combination with an existing active ingredient, a synergistic control effect can be obtained even for parasites that have acquired resistance. Effective components that can be used for this purpose include fulvalinate, acrinatrin, coumaphos or amitraz, and particularly preferred is fulvalinate or coumaphos. It is necessary to select an optimum mixing ratio depending on the mixing partner. Fenpyroximate Existing active ingredient should be selected in the range of 0.01 to 100 parts by weight, preferably 0.1 to 1 part by weight. The range is from 10 parts by weight to 10 parts by weight. These parasite control compositions can be produced and used in the same manner as in the case where the fenpyroximate alone is used as an active ingredient.

Example

[0015] The present invention will be specifically described below with reference to Examples and Test Examples, but the present invention is not limited to these.

Example 1.

Fenpyroximate 10 parts

Hydrous silicate 30 parts

No, Itenol N08 (Daiichi Kogyo Seiyaku) 5 parts

Lignin / Rehon acid power / Rethum 3 parts

52 parts wettable clay

After impregnating the active ingredient compound with hydrous silicic acid, uniformly mix with other ingredients to make a wettable powder. Example 2.

Fenpyroximate 20 parts Solpol 3105 (manufactured by Toho Chemical Co., Ltd.) 5 parts Propylene glycol 5 parts Rhodopol 23 (manufactured by Rhone Pulane) 2 parts Water 68 parts

The above was mixed uniformly and dispersed in water to obtain a flowable agent.

[0016] Example 3.

Fenpyroximate 10 parts

SP— 3005X (Toho Chemical Industries) 15 parts Xylene 55 parts

More than 20 parts of N-methylpyrrolidone was uniformly mixed and melted to prepare an emulsion.

Example 4.

Fenpyroximate 10 parts Fulvalinate 10 parts Sonoreponore 3105 5

Propylene glycolate 2 parts Rhodoponole 23 1 part

72 parts of water

[0017] Example 5.

Fenpyroximate 5 parts Fluvalinate 5 parts Hydrous silicate 34 parts Hightenol N08 3 parts Demol T (made by Kao Atlas) 2 parts Calcium carbonate powder 51 parts The active ingredient compound was impregnated with hydrous silicic acid and then mixed uniformly with other ingredients to obtain a wettable powder.

Example 6.

Fenpyroximate 10 parts

Kumahos 15 parts

SP-3005X 15 parts

40 parts of xylene

N-methylpyrrolidone 20 parts

The above was uniformly mixed and melted to obtain an emulsion.

[0018] Example 7.

Fenpyroximate 10 parts

Coumaphos 20 copies

Sonoreponore 3105 5¾

2 parts propylene glycol

Rhodoponole 23 0.5

Water 62. 5 咅 | 5

Mix the above uniformly and disperse in water to make a flowable agent.

Example 8.

Fenpyroximate 5 parts

80 parts of clay powder

Diatomaceous earth powder 15 parts

The above was uniformly mixed and pulverized to obtain a powder.

[0019] Example 9.

Fenpyroximate 3 parts

Full Validate 2 parts

80 parts of clay powder

Diatomaceous earth powder 15 parts

The above was uniformly mixed and pulverized to obtain a powder. Example 10.

Fenpyroximate 5 parts

10 coumaphos

Bentonite and clay mixed powder 80 parts

5 parts of lignins norephonate canoresium

The above mixture was mixed uniformly, mixed with an appropriate amount of water, granulated and dried to obtain granules.

[0020] Example 11.

Fenpyroximate 5 parts

50 parts of propylene glycol

Sodium carbonate 5 parts

40 parts of water

The above was uniformly mixed to obtain a high concentration solution. When used, it was diluted 1000 times with water. Example 12.

Fenpyroximate 5 parts

25 parts dioctyl phthalate

Castor oil 2 parts

Polychlorinated bur 68 parts

The above was mixed uniformly and formed into a sheet. When used, it was cut into an appropriate shape and placed in a bee hive.

[0021] Test Example 1. Sprinkling treatment (Taka fruit by

49-: 113 honeybees (Apis mellifera) infected with honeybee mite (Varroa destructor) were released in cages (12 X 8 X 20 cm), and the drug was diluted with water to a predetermined concentration and sprayed. After 3 hours of treatment, honey was fed in cotton wool, and 2 days after the treatment, the number of deaths of honeybee and honeybee mite was measured. Table 1 shows the results of calculating the corrected survival rate of honeybees and the corrected mortality rate of honeybee mites according to the following formula.

1

Survival rate of untreated zone Survival rate of treated zone Net nematode rate = X 100

Survival rate of untreated area

[Formula 2]

Survival rate of untreated section Survival rate of one treated section

Adjusted survival rate = 100-X 100

Survival rate of untreated area

[0022] [Table 1]

Table 1

[0023] From the results in Table 1, it can be seen that the parasite control agent composition in the bee population of the present invention has a high effect on honey bee mites, which are parasites that are less affected by bees. . Moreover, an effect can further be heightened by mixing with an existing agent.

[0024] Test Example 2. Effect of placing a drug-treated carrier in a nest box

A polyethylene sheet containing 5% fenpyroximate was placed between the nest entrance and between the nests in Apis mellifera nest boxes infected with honey bee mites (Varroa destructor). Before treatment, an arbitrary 10 honeybees were extracted and the number of Giant ticks to the infected honeybees was measured. Thirty days after the treatment, any 10 honeybees were extracted again, and the number of honeybee mites was measured to determine the reduction rate. The results are shown in Table 2. Coumaphos and fulvalinate were used as control agents.

[0025] [Table 2] Table 2

From the results in Table 2, it can be seen that the parasite control agent composition in the bee population of the present invention has the same high effect as the control drug even when the carrier holding the composition is placed in the nest box. .

Claims

The scope of the claims

[1] A parasite control agent composition in a bee population containing fenpyroximate as an active ingredient.

[2] The parasite control composition according to claim 1, further comprising one or more compounds selected from fulvalinate, acrinatrin, coumaphos or amitraz as an active ingredient.

[3] The parasite control composition according to claim 1 or 2, wherein the parasite in the bee population is a Varroa destructor.

[4] The method for using the parasiticidal composition according to any one of claims 1 to 3, wherein an effective amount of the active ingredient is brought into contact with the bees.

[5] The method for using a parasiticide control composition according to any one of claims 1 to 3, wherein an effective amount of the active ingredient is mixed in water or feed.