WO2006027602A1 - Melangeur/reacteur a liquides et procede correspondant - Google Patents

Melangeur/reacteur a liquides et procede correspondant Download PDF

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Publication number
WO2006027602A1
WO2006027602A1 PCT/GB2005/003484 GB2005003484W WO2006027602A1 WO 2006027602 A1 WO2006027602 A1 WO 2006027602A1 GB 2005003484 W GB2005003484 W GB 2005003484W WO 2006027602 A1 WO2006027602 A1 WO 2006027602A1
Authority
WO
WIPO (PCT)
Prior art keywords
droplet
excitation
substrate
eigenfrequencies
frequencies
Prior art date
Application number
PCT/GB2005/003484
Other languages
English (en)
Inventor
Sebastien Courty
Laurent Costier
Guillaume Lagubeau
Thomas Tixier
Guillaume Fichet
Original Assignee
Cambridge University Technical Services Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge University Technical Services Ltd filed Critical Cambridge University Technical Services Ltd
Publication of WO2006027602A1 publication Critical patent/WO2006027602A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/80—Mixing by means of high-frequency vibrations above one kHz, e.g. ultrasonic vibrations
    • B01F31/86—Mixing by means of high-frequency vibrations above one kHz, e.g. ultrasonic vibrations with vibration of the receptacle or part of it
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2215/00—Auxiliary or complementary information in relation with mixing
    • B01F2215/04—Technical information in relation with mixing
    • B01F2215/0413—Numerical information
    • B01F2215/0436—Operational information
    • B01F2215/0454—Numerical frequency values
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00889—Mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00925—Irradiation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00925—Irradiation
    • B01J2219/0093—Electric or magnetic energy
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip

Definitions

  • the present invention relates to a device and method for mixing or reacting liquids within a small volume, such as a droplet, by generating an oscillating perturbation which propagates within the droplet.
  • the present invention further provides a method of mixing or reacting two or more components within a droplet, the method comprising the steps of: positioning the droplet upon a substrate; and generating one or more excitation frequencies and resonating the droplet at the one or more excitation frequencies via an excitation means, wherein the one or more excitation frequencies comprise one or more eigenfrequencies of the droplet.
  • the major interest in exciting surface eigenmodes is the internal flow it induces.
  • the trajectories of fluid particles are given by the gradient of the velocity potential grad ⁇ since the amplitude of oscillation is small.
  • Mixing can be optimised by the present invention by using several eigenfrequencies at the same time, and furthermore by controlling singularities and surrounding streamlines which appear as a function of the eigenfrequencies, which can be manipulated to create a compartmentalised mixer or reactor.
  • the present invention therefore provides a cheap, simple and efficient mixing device and method on a microscopic or macroscopic scale which is fully applicable to commercial applications such as stand alone mixers, chemical reactors, mixing in micro or macro arrays, microfluidic systems, and inkjet technology.
  • the device and method of the present invention can operate within a wide range of temperatures, and are preferably configured to accommodate droplet volumes ranging between nl (nanolitres) and ml (millilitres).
  • Figure 1 shows a liquid mixer or reactor device according to the present invention
  • Figures 3a-d illustrate the compartmentalisation of a droplet when it is resonated at four different eigenfrequencies according to the present invention
  • Figure 4 shows an example of the present invention as a continuous mixer/reactor; and Figure 5 shows a microfluidic device, where the device of the present invention is integrated into the mixing stage of the device.
  • the concept underlying the present invention is based on the eigenmodes of vibration of a droplet sitting on a substrate, the droplet having a free surface which contacts the surrounding medium.
  • the droplet must be provided with a free surface which contacts the surrounding medium (this is preferably gaseous, but can comprise a non-miscibie liquid).
  • the substrate can be an open or a closed container, but in the case of a closed container, the container cannot solely enclose the droplet or volume being mixed/reacted, but must also enclose a surrounding medium.
  • the substrate 2 can be permanently attached to the excitation device 3, or may be temporarily fixed, via screws, clips, double-sided tape or glue, for example, (not shown) to the excitation device 3.
  • the excitation device 3 which generates an oscillating perturbation on the droplet's surface, can be, for example, a laser (radiative pressure, temperature gradient), a piezoelectric device, an acoustic wave or a vibrating membrane.
  • the excitation device 3 generates periodical (square, sinusoidal or triangular) or non- periodical signals of variable amplitudes and frequencies, thereby causing the substrate and liquid droplet to oscillate.
  • These droplet oscillations result in the creation of internal flows inside the droplet, which mainly depend on the excitation frequencies. These flows can be controlled and hence exploited for a mixing process or in order to provide a compartmentalised reactor.
  • the device and method of the invention are non-invasive, cheap to manufacture and can be employed in a controlled atmosphere.
  • the invention can be employed to greatly reduce the mixing time and increase the efficiency when mixing small amounts of liquid within a droplet, and this efficiency can be improved further by exciting the droplet with a square signal or with a sum of frequencies.
  • exciting the droplet with, for example, a sinusoidal signal, the droplet can be compartmentalised into several areas determined by internal flows induced in the droplet, and can thereby act as a liquid reactor.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

La présente invention concerne un dispositif et un procédé permettant de réaliser un mélange ou une réaction entre plusieurs composants à l'intérieur d'une gouttelette. La gouttelette (1) étant placée sur un substrat (2), on utilise un excitateur (3) de façon à faire entrer la gouttelette en résonance à une ou plusieurs fréquences d'excitation, lesquelles fréquences comprennent une ou plusieurs fréquences propres de la gouttelette. Un générateur (4) produit les fréquences d'excitation considérées. L'invention concerne également un dispositif et un procédé permettant de détecter l'une au moins des fréquences propres d'une gouttelette.
PCT/GB2005/003484 2004-09-10 2005-09-09 Melangeur/reacteur a liquides et procede correspondant WO2006027602A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0420155.4A GB0420155D0 (en) 2004-09-10 2004-09-10 Liquid mixing/reactor device and method
GB0420155.4 2004-09-10

Publications (1)

Publication Number Publication Date
WO2006027602A1 true WO2006027602A1 (fr) 2006-03-16

Family

ID=33186831

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/003484 WO2006027602A1 (fr) 2004-09-10 2005-09-09 Melangeur/reacteur a liquides et procede correspondant

Country Status (2)

Country Link
GB (1) GB0420155D0 (fr)
WO (1) WO2006027602A1 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2013604A1 (fr) * 2006-05-02 2009-01-14 Monash University Concentration et dispersion de petites particules dans de petits volumes de fluide au moyen d'énergie acoustique
WO2009144209A1 (fr) * 2008-05-27 2009-12-03 Alifax Holding Spa Dispositif de mélange et procédé de mélange y relatif
WO2013192351A1 (fr) * 2012-06-20 2013-12-27 Bio-Rad Laboratories, Inc. Gouttelettes stabilisées pour le calibrage et l'essai
FR3011488A1 (fr) * 2013-10-07 2015-04-10 Aelorve Dispositif pour melanger un volume de liquide
CN113316717A (zh) * 2019-01-10 2021-08-27 因塔公司 用于通过声波分析流体的传感装置
WO2021197790A1 (fr) * 2020-04-03 2021-10-07 Universite Paris-Saclay Procede pour generer un ecoulement dans une microgoutte et dispositif pour la mise en œuvre du procede
US11298701B2 (en) 2018-11-26 2022-04-12 King Instrumentation Technologies Microtiter plate mixing control system
CN114682163A (zh) * 2022-03-24 2022-07-01 安徽大学 用于生物相容性液滴的输送装置、制造方法及其输送方法
US20230120357A1 (en) * 2020-05-22 2023-04-20 Politecnico Di Milano Device, method and composition for transfection of cells with nucleic acids

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10164357A1 (de) * 2001-12-28 2003-07-10 Advalytix Ag Titrationsverfahren
US20040066118A1 (en) * 2001-02-28 2004-04-08 Guido Pieper Micro-reactor
US20040115097A1 (en) * 2001-04-09 2004-06-17 Achim Wixforth Mixing deivce and mixing method for mixing small amounts of liquid

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040066118A1 (en) * 2001-02-28 2004-04-08 Guido Pieper Micro-reactor
US20040115097A1 (en) * 2001-04-09 2004-06-17 Achim Wixforth Mixing deivce and mixing method for mixing small amounts of liquid
DE10164357A1 (de) * 2001-12-28 2003-07-10 Advalytix Ag Titrationsverfahren

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8998483B2 (en) 2006-05-02 2015-04-07 Royal Melbourne Institute Technology Concentration and dispersion of small particles in small fluid volumes using acoustic energy
EP2013604A4 (fr) * 2006-05-02 2013-10-16 Melbourne Inst Tech Concentration et dispersion de petites particules dans de petits volumes de fluide au moyen d'énergie acoustique
EP2013604A1 (fr) * 2006-05-02 2009-01-14 Monash University Concentration et dispersion de petites particules dans de petits volumes de fluide au moyen d'énergie acoustique
WO2009144209A1 (fr) * 2008-05-27 2009-12-03 Alifax Holding Spa Dispositif de mélange et procédé de mélange y relatif
US10240187B2 (en) 2012-06-20 2019-03-26 Bio-Rad Laboratories, Inc. Stabilized droplets for calibration and testing
US9527049B2 (en) 2012-06-20 2016-12-27 Bio-Rad Laboratories, Inc. Stabilized droplets for calibration and testing
WO2013192351A1 (fr) * 2012-06-20 2013-12-27 Bio-Rad Laboratories, Inc. Gouttelettes stabilisées pour le calibrage et l'essai
FR3011488A1 (fr) * 2013-10-07 2015-04-10 Aelorve Dispositif pour melanger un volume de liquide
WO2015052403A1 (fr) 2013-10-07 2015-04-16 Aelorve Dispositif et procédé pour mélanger un volume de liquide
US11298701B2 (en) 2018-11-26 2022-04-12 King Instrumentation Technologies Microtiter plate mixing control system
CN113316717A (zh) * 2019-01-10 2021-08-27 因塔公司 用于通过声波分析流体的传感装置
WO2021197790A1 (fr) * 2020-04-03 2021-10-07 Universite Paris-Saclay Procede pour generer un ecoulement dans une microgoutte et dispositif pour la mise en œuvre du procede
FR3108898A1 (fr) * 2020-04-03 2021-10-08 Universite Paris-Saclay Procede pour generer un ecoulement dans une microgoutte et dispositif pour la mise en œuvre du procede
US20230120357A1 (en) * 2020-05-22 2023-04-20 Politecnico Di Milano Device, method and composition for transfection of cells with nucleic acids
CN114682163A (zh) * 2022-03-24 2022-07-01 安徽大学 用于生物相容性液滴的输送装置、制造方法及其输送方法
CN114682163B (zh) * 2022-03-24 2023-12-29 安徽大学 用于生物相容性液滴的输送装置、制造方法及其输送方法

Also Published As

Publication number Publication date
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