WO2006016528A1

WO2006016528A1 - Pipet tip

Info

Publication number: WO2006016528A1
Application number: PCT/JP2005/014382
Authority: WO
Inventors: Takashi Usui
Original assignee: Nikkyo Technos Co., Ltd.; Eisai R & D Management Co., Ltd.
Priority date: 2004-08-12
Filing date: 2005-08-05
Publication date: 2006-02-16
Also published as: JP2007296407A

Abstract

A pipet tip capable of effectively preventing clogging from occurring when a reagent is sucked while sufficiently preventing a specimen from being broken or damaged when the reagent is fed to the specimen. The pipet tip (10) comprises a base part (11), an intermediate part (12), and a tip part (13), and is convergently formed from one end (10b) to the other end (13a). The base part (11) is a portion where a pipet is installed and removed. Also, the tip part (13) is formed to be extended from the tip of the intermediate part (12), and the tip thereof is closed by a bottom wall. In addition, through holes (K, K) are drilled, oppositely to each other, in the peripheral wall of the tip part (13) near the bottom wall.

Description

Specification

Pipette tip

Technical field

[0001] The present invention relates to a pipette tip.

Background art

[0002] Various pipette tips used for dispensing reagents and liquid samples have been proposed. In particular, it is widely used that can be attached to and removed from the tip of a pipette that accurately dispenses a small amount of reagent or liquid sample and prevents cross-contamination between reagents or samples. And

[0003] The pipette tip of this type is generally formed in a generally hollow conical shape and tapered so that a reagent or a liquid sample can be sucked and discharged at the tip. A bore hole is formed, and the base end portion is formed so as to be attachable to the tip end portion of the pipette (for example, see Patent Documents 1 to 3).

Patent Document 1: Japanese Patent Laid-Open No. 2003-260372

Patent Document 2: JP-A-8-266913

Patent Document 3: JP-A-8-112537

Disclosure of the invention

Problems to be solved by the invention

However, when a reagent or sample is supplied to a container using the above-described conventional pipette tip, the reagent or sample sucked and held in the pipette tip is a subject that is easily discharged (jetted) directly into the container. In some cases, impact force was applied to the sample, resulting in breakage or damage. For example, when a reagent such as a pipette tip is injected into a container in which a sample has been stored in advance, the reagent is likely to be ejected vigorously with a vertical upward force on the sample. To avoid this, careful pipetting (pipetting) is required, which leads to poor workability.

[0005] In addition, the conventional pipette tip described above may cause inconvenience even when the reagent or sample is inhaled into the pipette tip not only when the reagent or sample is discharged. That is, for example, after washing gel fragments and fragments in a container, When sucking and raising the pipette tip, the gel was sucked together with the washing solution! / ヽ, and the pipette tip could be clogged. In order to avoid this, careful pipetting operation is required, which deteriorates workability.

[0006] Therefore, the present invention has been made in view of the circumstances, and when the reagent is supplied to the sample or the liquid sample is supplied to the reagent, the breakage and damage of the sample can be sufficiently prevented. The purpose of the present invention is to provide a pipette chip that can effectively prevent clogging when a reagent or sample is inhaled.

Means for solving the problem

[0007] In order to solve the above problems, a pipette tip according to the present invention is capable of sucking and holding a reagent or a sample solution inside and discharging the reagent or sample solution to the outside. When the reagent or the sample solution is discharged to the outside, the reagent or the sample solution is formed so as to be emitted in a direction crossing the extending direction of the pipette tip.

[0008] With this configuration, the reagent or sample solution held inside is in the direction in which the pipette tip extends (usually the direction toward the sample or reagent to which the reagent or sample solution is supplied, and the vertical direction The reagent or sample solution is ejected in a direction crossing the vertical direction (that is, a direction slightly inclined from the vertical direction), that is, in a direction different from the extending direction. Is prevented.

[0009] Further, the pipette tip according to the present invention is capable of sucking and holding a reagent or a liquid sample inside and discharging the reagent or liquid sample to the outside, and the reagent or liquid sample is contained inside. The reagent or liquid sample is formed so as to flow in from the direction intersecting the extending direction of the pipette tip.

[0010] In conventional pipette tips, when a reagent or liquid sample is inhaled, the tip of the pipette tip is usually immersed in the reagent or liquid sample, and the tip force of the pipette tip is extended (generally vertical). The reagent or liquid sample is introduced along the direction or slightly inclined from the vertical direction) and aspirated inside. On the other hand, in the pipette chip according to the present invention having the above-described configuration, the reagent and the liquid sample flow into the inside from the direction intersecting the extending direction of the pipette tip, that is, the direction different from the extending direction.

[0011] Therefore, in a conventional pipette tip, in a container in which a reagent or a liquid sample is stored, For example, if an object such as a gel piece is contained, it is easy to inhale the object directly with the tip force, whereas the pipette tip of the present invention can be used to directly inhale a powerful object. As a result, the flow of the liquid sample is difficult to occur, and as a result, it is possible to prevent such an object from being accidentally inhaled into the pipette tip.

Specifically, the pipette tip according to the present invention has a substantially cylindrical shape with one end closed, and has an opening in the peripheral wall on the one end side.

[0013] The pipette tip configured in this way has, for example, an opening in which the tip end of a pipette is attached to the other end which is an open end, and a reagent or a liquid sample is formed on one end side by the pipetting operation. It is sucked into the interior space of the pipette tip through the section and held. At this time, even if an object such as a gel piece is contained in the container in which the reagent or liquid sample is contained, the opening is provided in the peripheral wall, so the reagent or liquid sample is It is easy to flow in the lateral direction from above, and it is difficult for a strong object to get caught in the flow and move to the opening side.

[0014] The reagent and the liquid sample thus housed and held in the pipette tip are discharged to the outside through the opening similarly by pipetting. At that time, since the opening is provided in the peripheral wall of the pipette tip, the peripheral wall force that the reagent or liquid sample does not directly inject or eject toward the sample or reagent to be supplied is also directed to the circumferential direction. Then, after it is ejected, it stalls, or after colliding with the inner wall of the container in which the sample and the reagent are stored in advance, the sample and the reagent are supplied along the inner wall.

[0015] The shape of the pipette tip is not particularly limited. Specifically, for example, when the other end force is formed so that the diameter is gradually reduced toward one end, as in the shape of a cone, Easy to pipette and useful.

[0016] Also, it is preferable to provide a plurality of openings! In this way, the reagent and liquid sample are also sucked into the pipette tip by a plurality of opening forces, so that the flow of the reagent and liquid sample during suction is dispersed and averaged without being biased in a certain direction. Therefore, it is further suppressed that the object is sucked together with the reagent and the liquid sample. In addition, since the reagent and liquid sample are discharged (discharged) from the plurality of openings to the outside of the pipette tip, it is possible to prevent the reagent and liquid sample from being biased and supplied to the sample and reagent. Discharged As a result, the momentum of the reagent and the liquid sample is alleviated and the discharge speed is likely to decrease.

[0017] Further, it is more preferable that the total area of the openings is formed so as to be substantially equal to the opening area in a state where one end is not closed. More specifically, it is particularly preferable that the total area of the openings is in the range of 0.8 X S to 1.2 X S, where S is the opening area when one end is not closed.

In this way, the reagent or liquid sample is discharged to the outside of the pipette tip with substantially the same discharge pressure as when using a conventional pipette tip having no opening and one end opened. Therefore, the discharge speed of the reagent or the liquid sample is reduced as compared with the case where the opening area of the opening is smaller while maintaining a sufficient discharge pressure. In addition, the discharge amount can be controlled more easily than when the opening area of the opening is larger, and a large amount of reagent or liquid sample is prevented from being discharged to the sample or reagent at a time.

[0019] Furthermore, it is preferable that a notch or a notch is formed in the peripheral wall on the other end side.

[0020] With a powerful configuration, when a reagent or liquid sample is inhaled into a pipette tip, and when Z or pipette tip force is also discharged, for example, it is associated with them via a notch or notch. By fixing to a holder or gripper that can be stopped or fitted, the entire pipette chip is positioned so as to be always fixed in the circumferential direction and the height direction (axial direction). Therefore, it is possible to always inhale and discharge the reagent and liquid sample at a fixed position. Therefore, the reagent and the liquid sample can be discharged in a certain direction and flowed in from a certain direction, the pipette tip may come into contact with the sample or the reagent stored in the container in advance, or the sample, the reagent and the opening. It is possible to prevent the pipette tip from tilting too far or the pipette tip from tilting. As a result, it is possible to more reliably prevent the reagent or liquid sample force in the pipette tip from being directly sprayed onto the sample or reagent.

The invention's effect

According to the pipette tip of the present invention, when a reagent is supplied to a sample or a liquid sample is supplied to a reagent, breakage and damage of the sample can be sufficiently prevented. In addition, clogging during inhalation of reagents and samples can be effectively prevented. As a result, the operability at the time of pipetting can be remarkably improved. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, embodiments of the present invention will be described in detail. The following embodiments are examples for explaining the present invention, and are not intended to limit the present invention only to the embodiments. The present invention can be implemented in various forms without departing from the gist thereof. In addition, positional relationships such as up, down, left and right are based on the positional relationships shown in the drawings unless otherwise specified. Furthermore, the dimensional ratios in the drawings are not limited to the illustrated ratios.

FIGS. 1A and 1B are schematic views showing a preferred embodiment of a pipette tip according to the present invention, which are a front view and a side view, respectively. The pipette tip 10 includes a base portion 11, a middle portion 12, and a tip portion 13, and each of them is tapered so that the diameter gradually decreases from the other end 10b toward the one end 13a.

[0024] The base 11 has a diameter larger than that of the middle portion 12, and the tip of a pipette (not shown) is detachably fitted so that the pipette tip 10 is attached to and detached from the pipette. It has become. Further, the base 11 is provided with a stagger 14 slightly toward the inside from the other end 10b, and the tip of the pipette abuts there. Further, two notches 15 and 15 are formed at positions facing each other on the end portion of the base portion 11 on the middle portion 12 side.

The middle portion 12 is a portion that connects the base portion 11 and the distal end portion 13 and has a diameter smaller than that of the base portion 11 and is relatively long. The distal end portion 13 is formed so as to extend the distal end force of the middle portion 12, and the distal end (one end 13a of the pipette tip 10) is closed by a bottom wall (the lower wall in the drawing). In addition, through holes Κ and K (both opening portions) are formed in the peripheral wall near the bottom wall of the tip portion 13 at positions facing each other.

[0026] Here, the opening area of the through holes Κ, K is not particularly limited, but the total area of the through holes K, is substantially the same as the opening area S in a state where the one end 13a is not temporarily closed. It is preferably formed so as to be equivalent, and particularly preferably formed so as to have a value within the range of 0.8 XS to 1.2 XS.

Here, FIG. 2 is a front view (partially sectional view) showing a state in which the reagent is supplied from the pipette tip 10 to the biological sample 30 accommodated in the container 20. In this example, the reagent is inhaled into the pipette tip 10. Pipette tip 10 has notch 15 fitted to holder 40 Then, the middle portion 12 and the tip portion 13 are loosely inserted into the container 20. The holder 40 is fixed, and the pipette tip 10 held by the holder 40 is not rotated with respect to the container 20 and is placed at the same height position.

[0028] In this state, when the pipette (not shown) is operated so that the reagent in the pipette tip 10 is discharged, the reagent follows the direction indicated by the dotted line Y1 (the extending direction of the pipette tip). To the tip 13 and diverted in the vicinity of the bottom wall of the tip 13 in the direction indicated by the arrow Y2 in the figure (a direction crossing the extending direction of the pipette tip; a direction different from the extending direction). It divides into two hands so as to follow, and is emitted to the outside through the through holes K and K and discharged to the outside. The reagent discharged from the through-holes K, K is released and supplied to the biological sample 30 in the force container 20 that draws a parabolic locus corresponding to the discharge pressure and gravity.

[0029] According to the pipette tip 10 configured in this manner, the open end of the tip portion 13 is closed by the bottom wall, and the through holes K, K are formed in the peripheral wall near the bottom wall. When supplying the reagent contained and held in the chip 10 to the biological sample in the container 20, the ejection speed is not sufficiently reduced because the reagent is not directly ejected to the biological sample 30 (i.e., stalled). The sample is discharged and supplied to the sample. Therefore, since the impact applied to the biological sample 30 can be mitigated, the biological sample 30 can be sufficiently prevented from being damaged or damaged.

[0030] Further, two through holes Κ, K are provided opposite to each other. In other words, the through holes Κ, K are arranged at 180 ° intervals on the peripheral wall, so that Reagents are supplied from two opposite directions. Therefore, the reagent can be prevented from being supplied to the biological sample 30 only in a certain direction, the reagent can be supplied more uniformly to the biological sample 30, and the momentum of the discharged reagent can be reduced. This makes it easier to further reduce the discharge speed.

[0031] Further, the total area force of the through holes Κ and K is substantially equal to the opening area when the one end 13a is not closed, so that one end is opened without having an opening. Reagents and liquid samples are discharged to the outside with approximately the same discharge pressure as conventional pipette tips. Therefore, the discharge speed of the reagent or liquid sample can be reduced as compared with the case where the total area of the through holes K and K is smaller while maintaining a sufficient discharge pressure.

[0032] In addition, compared to the case where the total area of the through holes Κ and K is larger, the discharge amount can be controlled more easily. It is possible to prevent an amount of reagent or liquid sample from being discharged to the biological sample 30 at once (at a stroke). Therefore, since the impact applied to the biological sample 30 can be further reduced, it is possible to further prevent the biological sample 30 from being broken or damaged. Furthermore, as a result, when a reagent or liquid sample is discharged from the pipette tip 10, an excessively careful pipetting operation is not required, so that the operability can be improved.

[0033] Furthermore, the notch 15 is fitted in the holder 40, whereby the pipette tip 10 is always positioned in a constant direction in the circumferential direction and the height direction (axial direction). Therefore, it is possible to always inhale and discharge the reagent and the liquid sample under a fixed geometric condition in such a fixed state. Therefore, the reagent and the liquid sample can be discharged in a certain direction and can be flown from the certain direction, and the biological sample 30 accommodated in the container 20 and the pipette tip may be inadequately contacted. It is possible to prevent the distance between 30 and the through hole K from being too close and the pipette tip 10 from being tilted. As a result, it is possible to prevent the reagent in the pipette tip 10 from being directly sprayed onto the biological sample 30, thereby preventing the biological sample 30 from being broken or damaged more reliably. In addition, since the pipette tip 10 is fixed, the handling and operability of the pipette can be improved.

[0034] In addition, as described above, the pipette tip 10 is extremely useful when inhaling a reagent or the like that is removed by force when the reagent or the like is discharged from the pipette tip 10. For example, in FIG. 2, the biological sample 30 is a fragment or fragment of a gel containing protein or DNA, and the pipette tip 10 is also used to discharge the washing solution to wash the gel, and this time when the washing solution is inhaled, The two-way force is also sucked into the pipette tip 10 in the direction opposite to the direction indicated by the arrow Y2 in the figure. That is, the cleaning liquid flows into the pipette tip 10 from a direction (direction different from the extending direction) intersecting the extending direction of the pipette tip 10 (direction indicated by the dotted line Y1 in the drawing).

[0035] Since the upward force of the gel piece as the biological sample 30 flows into the pipette tip 10 in this way, the gel piece is inserted and moved to the through holes K, K side. Flow is prevented from occurring. Therefore, it is possible to prevent the through holes K and K from being clogged with the gel pieces.

[0036] In addition, since the two through holes Κ and K are formed, the flow of the cleaning liquid is dispersed as compared with the case where there is only one through hole. Therefore, the flow of the cleaning solution sucked into the pipette tip 10 The speed decreases, and clogging of the through holes Κ and Κ due to the gel pieces can be more effectively suppressed. In addition, as a result of the above, when a reagent or liquid sample is inhaled into the pipette tip 10, an excessively careful pipetting operation is not required.

[0037] It should be noted that the present invention is not limited to the above-described embodiment, and various modifications can be made without changing the gist thereof. For example, the pipette tip 10 may have a rectangular tube shape and does not necessarily have to be tapered. In addition, it is desirable to provide a plurality of through-holes, but the number of through-holes is not limited to the one shown in the figure. Furthermore, it is not desirable to provide the notch 15, but it may be provided in only one place or three or more places. Furthermore, the notch 15 may be a narrower notch, that is, a notch.

[0038] In addition, if the pipette tip 10 is used, it is possible to discharge a liquid sample with a lateral force, which is particularly useful when creating a density gradient in a density gradient centrifugation method (for example, sucrose density gradient centrifugation method). is there. Furthermore, the use of the pipette tip 10 makes it possible to collect a liquid sample from the lateral direction, which is particularly useful when collecting a liquid sample for the purpose of density gradient centrifugation (eg, sucrose density gradient centrifugation). It is.

Industrial applicability

[0039] As described above, the pipette tip according to the present invention has a substantially cylindrical shape with one end closed, and has an opening in the peripheral wall on the one end side. When supplying a sample, it is possible to sufficiently reduce the impact caused by ejection. Therefore, breakage and damage of the sample can be sufficiently prevented. In addition, clogging when a reagent or sample is inhaled can be effectively prevented. In addition, the operability and handling at the time of pipette operation can be greatly improved. Therefore, it can be widely used for the analysis of samples containing biomolecules and the preparation of samples therefor.

Brief Description of Drawings

[0040] [FIG. 1] (Α) and (Β) are schematic views showing a preferred embodiment of a pipette tip according to the present invention, and are a front view and a side view, respectively.

FIG. 2 is a front view (partially sectional view) showing a state in which a reagent is supplied from the pipette tip 10 to the biological sample 30 stored in the container 20.

Claims

The scope of the claims

[1] A pipette tip capable of inhaling and holding a reagent or liquid sample and discharging the reagent or liquid sample to the outside,

When the reagent or liquid sample is sucked into the inside, the reagent or liquid sample is formed so as to flow in from a direction intersecting the extending direction of the pipette tip.

Pipette tip.

[2] A pipette tip capable of inhaling and holding a reagent or liquid sample and discharging the reagent or liquid sample to the outside,

When the reagent or liquid sample is discharged to the outside, the reagent or liquid sample is formed so as to be emitted in a direction crossing the extending direction of the pipette tip.

Pipette tip.

[3] It has a substantially cylindrical shape with one end closed, and has an opening in the peripheral wall on the one end side.

Pipette tip.

[4] It has a substantially cylindrical shape with one end closed, and has an opening in the peripheral wall on the one end side.

The pipette tip according to claim 1 or 2.

[5] A plurality of the openings are provided.

The pipette tip according to claim 3 or 4.

[6] The total area of the openings is formed to be substantially equal to the opening area in a state where the one end is not closed temporarily.

The pipette tip according to any one of claims 3 to 5.

[7] A notch or notch is formed in the peripheral wall on the other end side.

The pipette tip according to any one of claims 3 to 6.