WO2006003659A2 - Delivery system for transdermal immunization - Google Patents
Delivery system for transdermal immunization Download PDFInfo
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- WO2006003659A2 WO2006003659A2 PCT/IL2005/000710 IL2005000710W WO2006003659A2 WO 2006003659 A2 WO2006003659 A2 WO 2006003659A2 IL 2005000710 W IL2005000710 W IL 2005000710W WO 2006003659 A2 WO2006003659 A2 WO 2006003659A2
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- virus
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- delivery system
- skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/18—Applying electric currents by contact electrodes
- A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
- A61N1/327—Applying electric currents by contact electrodes alternating or intermittent currents for enhancing the absorption properties of tissue, e.g. by electroporation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0002—Galenical forms characterised by the drug release technique; Application systems commanded by energy
- A61K9/0009—Galenical forms characterised by the drug release technique; Application systems commanded by energy involving or responsive to electricity, magnetism or acoustic waves; Galenical aspects of sonophoresis, iontophoresis, electroporation or electroosmosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/02—Details
- A61N1/04—Electrodes
- A61N1/0404—Electrodes for external use
- A61N1/0408—Use-related aspects
- A61N1/0412—Specially adapted for transcutaneous electroporation, e.g. including drug reservoirs
- A61N1/0416—Anode and cathode
- A61N1/042—Material of the electrode
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/02—Details
- A61N1/04—Electrodes
- A61N1/0404—Electrodes for external use
- A61N1/0408—Use-related aspects
- A61N1/0428—Specially adapted for iontophoresis, e.g. AC, DC or including drug reservoirs
- A61N1/0432—Anode and cathode
- A61N1/0436—Material of the electrode
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- the present invention relates to a delivery system for transdermal immunization.
- the invention relates to a delivery system for effective topical administration of antigenic agents in conjunction with an apparatus that generates micro-channels in the skin of a subject.
- the delivery system is useful for immunization against bacterial, viral, and fungal antigens and for treating tumors and allergies.
- Vaccination can be achieved through various routes of administration, including oral, nasal, intramuscular (IM), subcutaneous (SC), and intradermal (ID).
- IM intramuscular
- SC subcutaneous
- ID intradermal
- the majority of commercial vaccines are administered by IM or SC routes. In almost all cases, they are administered by conventional injection with a syringe and needle, though high velocity liquid jet-injectors have had some success.
- the skin is a known immune organ. Pathogens entering the skin are confronted with a highly organized and diverse population of specialized cells capable of eliminating microorganisms through a variety of mechanisms.
- Epidermal Langerhans cells are potent antigen-presenting cells. Lymphocytes and dermal macrophages can penetrate to the dermis. Keratinocytes and Langerhans cells express or can be induced to generate a diverse array of immunologically active compounds. Collectively, these cells orchestrate a complex series of events that ultimately control both innate and specific immune responses.
- the skin's primary barrier, the stratum corneum, is impermeable to hydrophilic and high molecular weight drugs and macromolecules such as proteins, naked DNA 5 and viral vectors. Consequently, transdermal delivery has been generally limited to the passive delivery of low molecular weight compounds ( ⁇ 500 daltons) with limited hydrophilicity.
- Active methods of transdermal delivery include iontophoresis, electroporation, sonophoresis (ultrasound), and ballistic delivery of solid drug-containing particles. Delivery systems using active transport (e.g., sonophoresis) are in development, and delivery of macromolecules is possible with such systems. However, at this stage, it is not yet known if these systems will allow successful and reproducible delivery of macromolecules in humans.
- active transport e.g., sonophoresis
- U.S. Patent No. 5,980,898 discloses a patch for transcutaneous immunization comprising a dressing, an immunizing antigen, and an adjuvant, whereby application of the patch to intact skin induces an immune response specific for the immunizing antigen.
- application of the patch comprising the antigen does not involve perforating the intact skin neither by sound nor by electrical energy.
- inducing the immune response against an immunizing antigen, particularly a protein, which is otherwise not immunogenic by itself when placed on the skin requires the presence of an adjuvant.
- the adjuvant according to U.S. Patent No. 5,980,898 is preferably an ADP-ribosylating exotoxin such as cholera toxin, heat-labile enterotoxin, or pertussis toxin.
- U.S. Patent No. 6,706,693 discloses methods of non-invasively inducing a systemic immune response comprising topically administering either a plasmid DNA and liposome complex vector or a DNA vector that encode a gene of interest and express a protein encoded by the gene of interest, to the skin of a mammal to induce systemic immune response to the protein.
- the DNA vectors may be adenovirus recombinants or DN A/adeno virus complexes.
- 2001/0006645 discloses a method for the transdermal delivery of a selected drug comprising the steps of treating a skin area with alpha hydroxy acid to exfoliate the skin area, providing a patch containing the selected drug and a vehicle for enhancing the transdermal delivery of the selected drug, and applying the patch to the treated skin area.
- the method according to U.S. Patent Publication No. 2001/0006645 is useful particularly for immunization or vaccination against, for example, diphteria toxin, hepatitis B, polio, and chicken pox.
- U.S. Patent Publication No. US 2002/0193729 discloses an intradermal vaccine delivery device comprising a microprojection array having a plurality of stratum corneum piercing microprojections, which cut holes in the stratum corneum by piercing the skin to a depth of less than 500 ⁇ m, and a reservoir containing an antigenic agent and an immune response augmenting adjuvant, the reservoir being positioned in agent and adjuvant transmitting relationship with the holes.
- U.S. Patent No. 6,595,947 claims a method for a single and immediate delivery of a substance to the epidermal tissue of skin to enhance the immune response comprising simultaneously disrupting only the stratum corneum but not the epidermis of the skin and delivering the substance to the epidermal tissue of the skin.
- simultaneous delivery of a substance and abrasion of the outer layers of the skin by scraping or rubbing enhances an immune response to the substance.
- the substance according to U.S. 6,595,947 can be a nucleic acid, amino acid, peptide or polypeptide.
- U.S. Patent Publication No. 2004/0028727 discloses a patch for transcutaneous immunization comprising a dressing, an antigen, and an adjuvant, wherein at least one of the antigen and the adjuvant ingredients is in dry form, and whereby application of the patch to intact skin induces an immune response specific for the antigen.
- the adjuvant is preferably an ADP- ribosylating exotoxin.
- PCT International Patent Applications WO 2004/039426; WO 2004/039427; and WO 2004/039428 all assigned to the applicant of the present application, disclose systems and methods for transdermal delivery of pharmaceutical agents. Specifically disclosed are hydrophilic anti-emetic agents, dried compositions comprising polypeptides and proteins, and water-insoluble drugs.
- the systems and methods disclosed in WO 2004/039426, WO 2004/039427, and WO 2004/039428 significantly increased the permeation of the pharmaceutical compositions to the blood. There is an unmet need for practical, reliable, and effective methods for delivering antigens into or through the skin to induce immunization.
- the present invention relates to a transdermal delivery system for immunization.
- the transdermal delivery system comprises an apparatus that generates a plurality of micro-channels in an area of the skin of a subject and a composition comprising an antigenic agent.
- the transdermal delivery system of the present invention does not require an adjuvant.
- the immunizing effect achieved by the system of the present invention is as efficient in the absence of an adjuvant as in its presence, and thus rescues the skin area to which the antigenic agent is applied from irritation, sensitization or toxic effects associated with the use of an adjuvant.
- a composition comprising an antigenic agent or a commercially available vaccine can be administered in conjunction with the apparatus of the present invention, as it is shown herein that the micro-channels generated by the apparatus of the present invention enable effective delivery of a vaccine into the subject's body and induction of an antigen-specific immune response.
- the delivery system of the present invention is highly useful for inducing an immune response against high molecular weight molecules.
- the immune response induced is not limited to one antibody subtype, but rather can include the production of several antibody subtypes, i.e., IgM, IgG, and IgA.
- treatment of an area of the skin of a subject with the apparatus of the present invention and subsequent topical application of an antigenic agent on the area of the skin of the subject increases the IgA and the IgG antibody titers specific to the antigenic agent and these titers are comparable or even higher than those obtained by conventional immunization routes, i.e., subcutaneous or intramuscular routes.
- the present invention provides a system for immunization or vaccination that avoids the need for injections.
- treatment of an area of the skin of a subject with the apparatus of the present invention and then topical application of an antigenic agent on the area of the skin of the subject results in earlier appearance of significant and detectable titers of IgG antibodies specific to the antigenic agent as compared to the time of appearance of antibodies subsequent to subcutaneous or intramuscular antigen administration.
- the system of the present invention is specifically advantageous.
- topical application of a solution comprising an antigenic agent on an area of the skin of a subject, which has been treated with the apparatus of the present invention elicits antigen specific IgG antibodies more efficiently than a patch comprising a dried antigenic agent that is applied on skin treated with said apparatus.
- treatment of skin with the apparatus of the present invention and then application of a patch comprising a dried antigenic agent on the treated skin is shown to be highly efficient in eliciting antigen specific IgA antibodies as compared to subcutaneous or intramuscular routes.
- the apparatus of the present invention in conjunction with a particular formulation of an antigenic agent is useful for manipulating the immune system.
- the present invention encompass a wide variety of bacterial antigens, viral antigens, fungal antigens and other high molecular weight agents capable of inducing an antigen-specific immune response.
- the principles of the present invention are exemplified herein below using ovalbumin, a 45 kDa protein, and inactivated influenza vaccine consisting of three strains originally isolated from humans.
- the present invention provides a transdermal delivery system for inducing an antigen-specific immune response comprising an apparatus for facilitating transdermal delivery of an antigen through an area of the skin of a subject, wherein the apparatus capable of generating a plurality of micro-channels in the area of the skin of the subject other than by mechanical means, and a composition comprising an immunogenically effective amount of an antigen.
- the present invention incorporates the techniques for creating micro-channels by inducing ablation of the stratum corneum by electrical energy including the devices disclosed in U.S. Patents Nos.
- the transdermal delivery system comprising the apparatus for facilitating transdermal delivery of an antigen through an area of the skin of a subject, said apparatus comprises: a. an electrode cartridge comprising a plurality of electrodes; b. a main unit comprising a control unit which is adapted to apply electrical energy between the plurality of electrodes when said plurality of electrodes are in vicinity of the skin, typically generating current flow or one or more sparks, enabling ablation of stratum corneum in an area beneath the electrodes, thereby generating the plurality of micro-channels.
- control unit of the apparatus comprises circuitry to control the magnitude, frequency, and/or duration of the electrical energy delivered to the electrodes, so as to control the current flow or spark generation, and thus the width, depth and shape of the plurality of micro-channels.
- the electrical energy is at radio frequency.
- the electrode cartridge comprising the plurality of electrodes generates a plurality of micro-channels having uniform shape and dimensions.
- the electrode cartridge is removable. The electrode cartridge can be discarded after one use, and as such it is designed for easy attachment to the main unit and subsequent detachment from the main unit.
- the antigen is selected from the group consisting of bacterial antigens, viral antigens, fungal antigens, protozoan antigens, tumor antigens, allergens, autoantigens, fragments, analogs and derivatives thereof.
- the bacterial antigen is derived from a bacterium selected from the group consisting of anthrax, Campylobacter, Vibrio cholera, Clostridia, Diphtheria, enterohemorrhagic E coli, enterotoxigenic E.
- the viral antigen is derived from a virus selected from the group consisting of adenovirus, ebola virus, enterovirus, hanta virus, hepatitis virus, herpes simplex virus, human immunodeficiency virus, human papilloma virus, influenza virus, measles (rubeola) virus, Japanese equine encephalitis virus, papilloma virus, parvovirus B 19, poliovirus, respiratory syncytial virus, rotavirus, St. Louis encephalitis virus, vaccinia virus, yellow fever virus, rubella virus, chickenpox virus, varicella virus, and mumps virus.
- a virus selected from the group consisting of adenovirus, ebola virus, enterovirus, hanta virus, hepatitis virus, herpes simplex virus, human immunodeficiency virus, human papilloma virus, influenza virus, measles (rubeola) virus, Japanese equine
- the fungal antigen is derived from a fungus selected from the group consisting of tinea corporis, tinea unguis, sporotrichosis, aspergillosis, and Candida.
- the protozoan antigen is derived from protozoa selected from the group consisting of Entamoeba histolytica, Plasmodium, and Leishmania.
- the antigen is selected from peptides, polypeptides, proteins, glycoproteins, lipoproteins, lipids, phospholipids, carbohydrates, glycolipids and conjugates thereof. It is to be understood that the composition can comprise two or more antigens.
- the composition comprising the antigen of the invention can be formulated in a dry formulation or liquid formulation.
- the dry formulation is a patch.
- composition comprising the antigen further comprises an adjuvant.
- the present invention provides a method for inducing transdermally an antigen-specific immune response in a subject comprising: (i) generating a plurality of micro-channels in an area of the skin of a subject other than by mechanical means; and
- composition comprising an immunogenically effective amount of an antigen and a pharmaceutically acceptable carrier to the area of the skin in which the plurality of micro-channels are present, thereby inducing an antigen-specific immune response.
- the plurality of micro-channels are generated by an apparatus comprising: a. an electrode cartridge comprising a plurality of electrodes; b. a main unit comprising a control unit which is adapted to apply electrical energy between the plurality of electrodes when said plurality of electrodes are in vicinity of the skin, typically generating current flow or one or more sparks, enabling ablation of stratum corneum in an area beneath the electrodes, thereby generating the plurality of micro-channels.
- the electrode cartridge comprising the plurality of electrodes is removable.
- the electrical energy is of radio frequency.
- the method for inducing an antigen-specific immune response comprises an antigen-specific antibody.
- the antigen-specific immune response comprises an antigen-specific lymphocyte.
- the method for transdermally inducing an immune response enables eliciting the response against a variety of antigenic agents such as bacterial antigens, viral antigens, fungal antigens, protozoan antigens, tumor antigens, allergens, and autoantigens
- the method of the present invention is useful for immunoprotection, immunosuppression, modulation of an autoimmune disease, potentiation of cancer immunosurveillance, prophylactic vaccination to prevent disease, and therapeutic vaccination to treat or reduce the severity and/or duration of established disease.
- FIG. 1 shows IgM plasma titers in guinea pigs 15 days after either primary subcutaneous immunization (S.C.) with ovalbumin or ViaDerm treatment followed by transdermal immunization with ovalbumin solution (VD-s).
- FIG. 2 shows IgG plasma titers in guinea pigs 15 days after either primary subcutaneous immunization (S.C.) with ovalbumin or ViaDerm treatment followed by transdermal immunization with ovalbumin solution (VD-s).
- FIGs. 3A-B show IgA and IgG plasma titers in guinea pigs 6 days after boost (day 36 after primary immunization).
- FIG. 3 A shows IgA and IgG plasma titers 6 days after boost (day 36 after primary immunization) by intramuscular immunization with ovalbumin solution (i.m.) or subcutaneous immunization (S.C.) with ovalbumin.
- FIG. 3B shows IgA and IgG plasma titers 6 days after boost (day 36) by ViaDerm treatment followed by transdermal immunization with either ovalbumin solution (VD-s) or ovalbumin powder (VD-p).
- VD-s ovalbumin solution
- VD-p ovalbumin powder
- FIG. 4 shows IgG plasma titers in guinea pigs 95 days after boost (125 days after primary vaccination) by either subcutaneous immunization (S. C.) with ovalbumin or ViaDerm treatment followed by transdermal immunization with ovalbumin solution (VD-s).
- FIG. 5 shows IgA plasma titers in guinea pigs 15 days after either primary subcutaneous immunization (S. C.) with ovalbumin or ViaDerm treatment followed by transdermal immunization with ovalbumin solution (VD-s).
- FIG. 6 shows IgA plasma titers in guinea pigs 12 days after boost (day 42 after primary immunization) by either subcutaneous immunization (S.C.) with ovalbumin or ViaDerm treatment followed by transdermal immunization with ovalbumin solution (VD-s).
- FIG. 7 shows Trans Epidermal Water Loss (TEWL) values in guinea pigs treated with either 50-micron or 100-micron length electrodes of ViaDerm and control guinea pigs.
- FIG. 8 shows serum IgG antibody titers against A/Panama strain of influenza in guinea pigs treated with either 50-micron or 100-micron length electrodes of ViaDerm and then immunized with the influenza vaccine patch in the absence or presence of E. coli heat labile enterotoxin (LT).
- LT E. coli heat labile enterotoxin
- a control group was immunized with the influenza vaccine patch in the absence or presence of LT.
- a group of guinea pigs immunized intramuscularly with the influenza vaccine and then boosted intramuscularly with the same vaccine is also shown.
- FIG. 9 shows serum IgG antibody titers against A/Caledonia strain of influenza in guinea pigs treated with either 50-micron or 100-micron length electrodes of ViaDerm and then immunized with the influenza vaccine patch in the absence or presence of LT.
- a control group was immunized with the influenza vaccine patch in the absence or presence of LT.
- a group of guinea pigs immunized intramuscularly with the influenza vaccine and then boosted intramuscularly with the same vaccine is also shown.
- FIG. 10 shows serum IgG antibody titers against B/Shangdong strain of influenza in guinea pigs treated with either 50-micron or 100-micron length electrodes of ViaDerm and then immunized with the influenza vaccine patch in the absence or presence of LT.
- a control group was immunized with the influenza vaccine patch in the absence or presence of LT.
- a group of guinea pigs immunized intramuscularly with the influenza vaccine and then boosted intramuscularly with the same vaccine is also shown.
- the present invention provides transdermal delivery system for inducing an antigen-specific immune response comprising an apparatus for facilitating transdermal delivery of an antigenic agent through the skin of a subject, said apparatus capable of generating at least one micro-channel in an area on the skin of the subject and a composition comprising an immunogenically effective amount of at least one antigenic agent.
- antigenic agent and “antigen”, used interchangeably throughout the specification and claims, refer to an active component of the composition, which is specifically recognized by the immune system of a human or animal subject after immunization or vaccination.
- the antigen can comprise a single or multiple immunogenic epitopes recognized by a B-cell receptor (i.e., secreted or membrane- bound antibody) or a T cell receptor.
- B-cell receptor i.e., secreted or membrane- bound antibody
- T cell receptor i.e., secreted or membrane- bound antibody
- the antigenic agent according to the present invention is also an immunogenic agent.
- An “immunogenic” agent refers to an agent that is capable of inducing an antigen specific immune response.
- immuno and “vaccination” refer to the induction of an antigen specific immune response and are used interchangeably throughout the specification and claims.
- An antigen can be a peptide, a polypeptide, a protein, a glycoprotein, a lipoprotein, a lipid, a phospholipid, a carbohydrate, a glycolipid, a mixture or a conjugate thereof, or any other material known to induce an immune response.
- the molecular weight of the antigen may be greater than 1 kilodalton (kDa), 10 kDa or 100 kDa (including intermediate ranges thereof).
- An antigen can be conjugated to a carrier.
- An antigen can be provided as a whole organism such as, for example, a bacterium or virion; an antigen can be obtained from an extract or lysate of organisms, e.g., from whole cells or from membranes; an antigen can be provided as live organisms such as, for example, live viruses or bacteria, attenuated live organisms such as, for example, attenuated live viruses or bacteria, or organisms that have been inactivated by chemical or genetic techniques; and an antigen can be chemically synthesized, produced by recombinant technology or purified from natural sources.
- a "peptide” refers to a polymer in which the monomers are amino acids linked together through amide bonds. Peptides are generally smaller than polypeptides, typically under 30-50 amino acids in total.
- polypeptide refers to a single polymer of amino acids, generally over 50 amino acids.
- a “protein” as used herein refers to a polymer of amino acids typically over 50 amino acids comprising one or more polypeptide chains.
- Antigenic peptides or polypeptides include, for example, natural, synthetic or recombinant B-cell or T-cell epitopes, universal T-cell epitopes, and mixed T-cell epitopes from one organism or disease and B-cell epitopes from another.
- Antigens obtained through recombinant technology or peptide synthesis as well as antigens obtained from natural sources or extracts can be purified by purification methods based on the physical and chemical characteristics of the antigens, preferably by fractionation or chromatography. Peptide synthesis is well known in the art and is available commercially from a variety of companies.
- a peptide or polypeptide can be synthesized using standard direct peptide synthesis (e.g., as summarized in Bodanszky, 1984, Principles of Peptide Synthesis (Springer- Verlag, Heidelberg), such as via solid-phase synthesis (see, e.g., Merrifield, 1963, J. Am. Chem. Soc. 85:2149-2154).
- Recombinant antigens can combine one or more antigens.
- An antigen composition comprising one or more antigens can be used to induce an immune response to more than one antigen at the same time.
- Such recombinant antigens can be made by ligating the appropriate nucleic acid sequences encoding the desired amino acid sequences to each other by methods known in the art, in the proper coding frame, and expressing the recombinant antigens by methods commonly known in the art (see, for example, Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, 2d edition, Cold Spring Harbor Press).
- a multivalent antigen composition can be used to induce an immune response to more than one immunogenic epitope in one antigenic agent.
- Conjugates can also be used to induce an immune response to multiple antigens, to boost the immune response, or both.
- conjugates can be made by protein synthesis, e.g., by use of a peptide synthesizer. Fragments of antigens can be also used to induce an immune response.
- antigens can be used to vaccinate a subject and to induce an immune response specific for the antigen.
- the antigen can be derived from a pathogen that can infect a subject.
- antigens can be derived from, for example, bacteria, viruses, fungi, or parasites.
- the antigen can be a tumor antigen.
- the antigen can be an allergen including, but not limited to, pollen, animal dander, mold, dust mite, flea allergen, salivary allergen, grass, or food (e.g., peanuts and other nuts).
- the antigen can be an autoantigen.
- the autoantigen can be associated with an autoimmune disease such as, for example, the pancreatic islet antigen.
- Antigens can be derived from bacteria.
- bacteria include, but are not limited to, anthrax, Campylobacter, Vibrio cholera, Clostridia including Clostridium difficile, Diphtheria, enterohemorrhagic E. coli, enterotoxgenic E.
- coli Giardia, gonococcus, Helicobacter pylori, Hemophilus influenza B, Hemophilus influenza non- typeable, Legionella, meningococcus, Mycobacteria including those organisms responsible for tuberculosis, pertussis, pneumococcus, salmonella, shigella, staphylococcus, Group A beta-hemolytic streptococcus, Streptococcus B, tetanus, Borrelia burgdorfi, Yersinia, and a like.
- bacterial antigens include, for example, toxins, toxoids (i.e., chemically inactivated toxins, which are less toxic but retain immunogenicity), subunits or combinations thereof, and virulence or colonization factors.
- Bacterial constituents, products, lysates and/or extracts can be used as a source for bacterial antigens.
- Antigens can be derived from viruses.
- Viruses include, but are not limited to, adenovirus, dengue serotypes 1 to 4 virus, ebola virus, enterovirus, hanta virus, hepatitis virus serotypes A to E, herpes simplex virus 1 or 2, human immunodeficiency virus, human papilloma virus, influenza virus, measles (rubeola) virus, Japanese equine encephalitis virus, papilloma virus, parvovirus B 19, poliovirus, rabies virus, respiratory syncytial virus, rotavirus, St.
- Viral constituents, products, lysates and/or extracts can be used as a source for the viral antigens.
- Antigens can be derived from fungi.
- Fungi include, but are not limited to, tinea corporis, tinea unguis, sporotrichosis, aspergillosis, Candida, and other pathogenic fungi.
- Fungal constituents, products, lysates and/or extracts can be used as a source for the fungal antigens.
- Antigens can be produced from protozoans.
- Protozoans include, for example, Entamoeba histolytica, Plasmodium, and Leishmania.
- Protozoan constituents, products, lysates and/or extracts can be used as a source for the protozoan antigens.
- Vaccination can be also used as a treatment for cancer, allergies, and autoimmune diseases.
- a tumor antigen e.g., HER2, prostate specific antigen
- Tumor antigens useful for vaccination are known in the art and include, for example, tumor antigens of leukemia, lymphoma, and melanoma.
- Vaccination with T-cell receptors or autoantigens can induce an immune response that halts progression of an autoimmune disease.
- the present invention encompasses fragments, derivatives, and analogs of the antigenic agents so long as the fragments, derivatives, and analogs being immunogenic and thereby capable of inducing an antigen specific immune response.
- Fragments of an antigenic agent can be produced by subjecting the antigen to at least one cleavage agent.
- a cleavage agent can be a chemical cleavage agent, e.g., cyanogen bromide, or an enzyme, e.g., endoproteinase, exoproteinase, or lipase.
- protein antigenic agents can be modified by derivatization reactions including, but not limited to, oxidation, reduction, myristylation, sulfation, acylation,
- Such alterations, which do not destroy the immunogenic epitope of an antigen can occur anywhere in the antigen. It will be appreciated that one or more modifications can be present in the same antigen.
- analog refers to antigenic agents comprising altered sequences by amino acid substitutions, additions or deletions.
- the present invention provides highly effective systems and methods for transdermal delivery of antigenic agents without the use of adjuvants.
- the present invention also encompasses compositions comprising an antigen and an adjuvant.
- activation of antigen presenting cells by an adjuvant occurs prior to presentation of an antigen.
- an antigen and an adjuvant can be separately presented within a short interval of time but targeting the same anatomical region.
- adjuvant refers to a substance that is used to specifically or nonspecif ⁇ cally potentiate an antigen-specific immune response.
- adjuvant activity is the ability to increase the immune response to an antigen (i.e., an antigen which is a separate chemical structure from the adjuvant) by inclusion of the adjuvant in a composition.
- Adjuvants include, but are not limited to, an oil emulsion (e.g., complete or incomplete Freud's adjuvant), chemokines (e.g., defensins, HCC-I, HCC-4, MCP-I, MCP-3, MCP-4, MlP-lct, MlP-l ⁇ , MlP-l ⁇ , MIP-3 ⁇ , and MIP-2); other ligands of chemokine receptors (e.g., CCR-I, CCR-2, CCR-5, CCR-6, CXCR-I); cytokines (e.g., IL-I, IL-2, IL-6, IL-8, IL-10, IL-12, IFN- ⁇ ; TNF- ⁇ , GM-CSF); other ligands of receptors for these cytokines, immunostimulatory CpG motifs of bacterial DNA or oligonucleotides; muramyl dipeptide (MDP) and derivatives thereof
- ADP-ribosylating exotoxins are organized as A:B heterodimers with a B subunit containing the receptor binding activity and an A subunit containing the ADP-ribosyltransferase activity.
- Exemplary bARE include cholera toxin (CT), E. coli heat-labile enterotoxin (LT), diphtheria toxin, Pseudomonas exotoxin A (ETA), pertussis toxin (PT), C. botulinum toxin C2, C. botulinum toxin C3, C. limosum exoenzyme, B. cereus exoenzyme, Pseudomonas exotoxin S, S. aureus EDIN, and B. sphaericus toxin.
- Mutant bARE containing mutations of the trypsin cleavage site or mutations affecting ADP-ribosylation may be used.
- adjuvants such as bARE are known to be highly toxic when injected or given systemically. But if placed on the surface of intact skin or penetrate to the epidermis, they can provide adjuvant effects without systemic toxicity (see, for example, U.S. Patent Application Publication Nos. 2004/0258703 and 2004/0185055, incorporated by reference as if fully set for the herein).
- Adjuvant can be chosen to preferentially induce specific antibodies (e.g., IgM, IgD, IgAl, IgA2, IgE, IgGl, IgG2, IgG3, and/or IgG4), or specific T-cell subsets (e.g., CTL, ThI, and/or Th2).
- Unmethylated CpG dinucleotides or similar motifs are known to activate B lymphocytes and macrophages.
- Other forms of bacterial DNA can be used as adjuvants.
- bacterial DNA belongs to a class of structures, which have patterns allowing the immune system to recognize their pathogenic origin and to stimulate the innate immune response leading to adaptive immune responses. These structures are called pathogen-associated molecular patterns (PAMP) and include lipopolysaccharides, teichoic acids, unmethylated CpG motifs, double stranded RNA, and mannins. PAMP induce endogenous signals that can mediate the inflammatory response and can act as co-stimulators of T-cell function.
- PAMP pathogen-associated molecular patterns
- Adjuvants can be biochemically purified from a natural source, can be produced synthetically or recombinantly produced.
- the adjuvants according to the present invention include truncations, substitutions, deletions, and additions of the natural occurring adjuvants so long as the adjuvant activity is retained.
- Vaccine components in the presence of water are chemically less stable and more prone to contamination through the provision of an aqueous medium for the growth of bacteria.
- the stringent requirement for cold storage during transport and storage of vaccines has led to the ⁇ cold chain', indicating that at all times after manufacture of the vaccine, the vaccine is kept in proper cold storage conditions. This increases the complexity of storing vaccine, creates logistical problems when transporting vaccine, and adds greatly to the expense of vaccination.
- compositions useful for immunization or vaccination contain an immunogenically effective amount of at least one antigenic agent and a pharmaceutically acceptable carrier or vehicle in order to provide pharmaceutical- acceptable compositions suitable for administration to a subject (i.e., human or animal).
- a pharmaceutically acceptable carrier or vehicle in order to provide pharmaceutical- acceptable compositions suitable for administration to a subject (i.e., human or animal).
- pharmaceutically acceptable means approved by a regulatory agency of the Federal or a state government or listed in the U. S. Pharmacopeia or other generally recognized pharmacopeia for use in animals, and more particularly in humans.
- carrier refers to a diluent, excipient, or vehicle with which the therapeutic compound is administered.
- antigens can be solubilized in a buffer or water, or incorporated in emulsions, lipid micelles or vesicles.
- Suitable buffers include, but are not limited to, phosphate buffered saline (PBS), phosphate buffered saline Ca++/Mg++ free, normal saline (150 mM NaCl in water), Hepes or Tris buffer.
- Antigens, which are not soluble in neutral buffer can be solubilized in 10 mM acetic acid and then diluted to the desired volume with a neutral buffer such as PBS.
- acetate-PBS at acidic pH can be used as a diluent after solubilization in dilute acetic acid.
- Other useful carriers include, for example, ethanol, ethylene glycol, propylene glycol, butane- 1, 3-diol, isopropyl myristate, isopropyl palmitate, or mineral oil. Methodology and components for formulation of pharmaceutical compositions are well known, and can be found, for example, in Remington's Pharmaceutical Sciences, Eighteenth Edition, A. R. Gennaro, Ed., Mack Publishing Co. Easton Pa., 1990.
- Stabilizers include, but are not limited to, dextrans and dextrins, glycols, alkylene glycols, polyalkane glycols, polyalkylene glycols, sugars, starches, and derivatives thereof.
- Preferred additives are non-reducing sugars and polyols.
- glycerol, trehalose, hydroxymethyl or hydroxyethyl cellulose, ethylene or propylene glycol, trimethyl glycol, vinyl pyrrolidone, and polymers thereof can be added.
- Alkali metal salts, ammonium sulfate, and magnesium chloride can stabilize proteinaceous antigens.
- a polypeptide can also be stabilized by contacting it with a sugar such as, for example, a monosaccharide, disaccharide, sugar alcohol, and mixtures thereof (e.g., arabinose, fructose, galactose, glucose, lactose, maltose, mannitol, mannose, sorbitol, sucrose, xylitol).
- a sugar such as, for example, a monosaccharide, disaccharide, sugar alcohol, and mixtures thereof (e.g., arabinose, fructose, galactose, glucose, lactose, maltose, mannitol, mannose, sorbitol, sucrose, xylitol).
- Polyols can also stabilize a polypeptide.
- Various other excipients can also stabilize polypeptides including amino
- compositions of the invention can be formulated as a dry or liquid formulation.
- a dry formulation is more easily stored and transported than conventional liquid vaccines, as it breaks the cold chain required from the vaccine's place of manufacturing to the location where vaccination occurs.
- a dry formulation can be more advantageous than liquid formulations since high concentrations of a dry active component of the composition (e.g., one or more antigens) can be achieved by solubilization directly at the site of immunization over a short time span. Moisture from the skin and an occlusive backing layer can hasten this process.
- the composition can be provided as a liquid formulation including, but not limited to, solution, suspension, emulsion, cream, gel, lotion, ointment, paste, or other liquid forms.
- the composition can be provided as a dry formulation. Dry formulations include, but not limited to, fine or granulated powders, uniform films, pellets, tablets and patches.
- the formulation may be dissolved and then dried in a container or on a flat surface (e.g., skin), or it may simply be dusted on the flat surface. It may be air dried, dried with elevated temperature, freeze or spray dried, coated or sprayed on a solid substrate and then dried, dusted on a solid substrate, quickly frozen and then slowly dried under vacuum, or combinations thereof. If more than one antigenic agent is included in a composition, the antigenic agents can be mixed in solution and then dried, or mixed in a dry form only.
- the composition can be provided in a form of a patch.
- a "patch” refers to a product, which comprises an antigenic agent and a solid substrate, typically a backing layer, which functions as the primary structural element of the patch (see, for example, WO 02/074244 and WO 2004/039428, incorporated by reference as if fully set forth herein).
- a patch can further comprise an adhesive and/or a microporous liner layer.
- the microporous liner layer is a rate-controlling matrix or a rate-controlling membrane that allow extended release of the antigenic agent.
- a liquid formulation can be incorporated in a patch (i.e., a wet patch).
- the liquid formulation can be held in a reservoir or can be mixed with the contents of a reservoir.
- a wet patch can contain a single reservoir containing one antigenic agent, or multiple reservoirs to separate individual antigenic agents.
- a patch can also be a dry patch.
- a dry patch can be a powder patch such as, for example, a printed patch as disclosed in WO 2004/039428 or any other dry patch known in the art (see Examples herein below); applying a powder patch allows control over the time and rate of the dissolution of the antigenic agent.
- a dry patch can include one or more dried antigenic agents such that application of a patch, whether a wet or dry patch, comprising multiple antigens induces an immune response to the multiple antigens. In such a case, antigens can or cannot be derived from the same source, but will have different chemical structures so as to induce an immune response specific for the different antigens.
- the backing layer can be non-woven or woven (e.g., gauze dressing). It may be non-occlusive or occlusive, but the latter is preferred.
- the optional release liner preferably does not adsorb significant amounts of the composition.
- the patch is preferably hermetically sealed for storage (e.g., foil packaging). The patch can be held onto the skin and components of the patch can be held together using various adhesives.
- One or more of the antigens may be incorporated into the substrate or adhesive parts of the patch.
- patches are planar and pliable, and they are manufactured with a uniform shape.
- Optional additives are plasticizers to maintain pliability of the patch, tackifiers to assist in adhesion between patch and skin, and thickeners to increase the viscosity of the formulation at least during processing.
- Metal foil cellulose, cloth (e.g., acetate, cotton, rayon), acrylic polymer, ethylenevinyl acetate copolymer, polyamide (e.g., nylon), polyester (e.g., polyethylene naphthalate, ethylene terephthalate), polyolefin (e.g., polyethylene, polypropylene), polyurethane, polyvinyl alcohol, polyvinyl pyrrolidone, polyvinylidene chloride
- SARAN synthetic rubber
- silicone elastomer silicone elastomer
- patch materials e.g., backing layer, release liner
- the adhesive may be an aqueous-based adhesive (e.g., acrylate or silicone).
- Acrylic adhesives available from several commercial sources, are sold under the trade names AROSET, DUROTAK, EUDRAGIT, GELVA, and NEOCRYL.
- the acrylic polymer may be co-polymerized with hydrophilic monomer, monomer containing carboxyl group, monomer containing amide group, monomer containing amino group, and the like. Rubbery or silicone resins may be employed as the adhesive resin; they may be incorporated into the adhesive layer with a tackifying agent or other additives.
- the water absorption capacity of the adhesive layer can be also regulated by incorporating therein highly water-absorptive polymers, polyols, and water-absorptive inorganic materials.
- highly water-absorptive resins may include mucopolysaccharides such as hyaluronic acid, chondroitin sulfate, dermatan sulfate and the like; polymers having a large number of hydrophilic groups in the molecule such as chitin, chitin derivatives, starch and carboxy-methylcellulose; and highly water-absorptive polymers such as polyacrylic, polyoxyethylene, polyvinyl alcohol, and polyacrylonitrile.
- the plasticizer may be a trialkyl citrate such as, for example, acetyl-tributyl citrate (ATBC), acetyl-triethyl citrate (ATEC), and triethyl citrate (TEC).
- ATBC acetyl-tributyl citrate
- ATEC acetyl-triethyl citrate
- TEC triethyl citrate
- exemplary tackifiers are glycols (e.g., glycerol, 1,3 butanediol, propylene glycol, polyethylene glycol).
- Succinic acid is another tackifier.
- Thickeners can be added to increase the viscosity of an adhesive or immunogenic composition.
- the thickener may be a hydroxyalkyl cellulose or starch, or water-soluble polymers: for example, poloxamers, polyethylene oxides and derivatives thereof, polyethyleneimines, polyethylene glycols, and polyethylene glycol esters.
- any molecule which serves to increase the viscosity of a solution may be suitable to improve handling of a formulation during manufacture of a patch.
- Gel and emulsion systems can be incorporated into patch delivery systems, or be manufactured separately from the patch, or added to the patch prior to application to the human or animal subject. Gels or emulsions may serve the same purpose of facilitating manufacture by providing a viscous formulation that can be easily manipulated with minimal loss.
- the term "gel” refers to covalently cross-linked, non cross-linked hydrogel matrices. Hydrogels can be formulated with at least one antigenic agent.
- excipients may be added to the gel systems that allow for the enhancement of antigen delivery, skin hydration, and protein stability.
- emulsion refers to formulations such as water-in-oil creams, oil-in-water creams, ointments, and lotions.
- Emulsion systems can be either micelle-based, lipid vesicle-based, or both micelle- and lipid vesicle-based.
- a liquid formulation may be applied directly to the skin and allowed to air dry or held in place with a dressing, patch, or absorbent material.
- the formulation may be applied in an absorbent dressing or gauze.
- the formulation may be covered with an occlusive dressing such as, for example, AQUAPHOR (an emulsion of petrolatum, mineral oil, mineral wax, wool wax, panthenol, bisabol, and glycerin from Beiersdorf), plastic film, COMFEEL (Coloplast) or VASELINE petroleum jelly; or a non-occlusive dressing such as, for example, TEGADERM (3M), DUODERM (3M) or OPSITE
- each unit dose can contain one or more antigenic agents in predetermined amounts for a single round of immunization.
- the amount of an antigenic agent in the unit dose can range from about 0.1 ⁇ g to about 10 mg.
- compositions of the present invention can be manufactured under good manufacturing practices regulated by government agencies (e.g., Food and Drug Administration) for biologicals and vaccines.
- the system of the present invention comprises an apparatus for enhancing transdermal immunization.
- the apparatus is used to generate at least one micro-channel in an area on the skin of a subject through which a composition comprising an antigenic agent is delivered efficiently.
- micro-channel refers to a pathway, generally extending from the surface of the skin through all or significant part of the stratum corneum, through which molecules can diffuse.
- the apparatus for facilitating transdermal movement of an antigenic agent is as disclosed in one or more of the U.S. Pat. Nos. 6,148,232; 6,597,946; 6,611,706; 6,711,435; 6,708,060; and 6,615,079, the contents of which is incorporated by reference as if fully set forth herein.
- the apparatus comprises an electrode cartridge comprising a plurality of electrodes, and a main unit comprising a control unit adapted to apply electrical energy between the plurality of electrodes when the electrodes are in vicinity of the skin, typically generating current flow or one or more sparks, enabling ablation of stratum corneum in an area beneath the electrodes, thereby generating at least one micro- channel.
- the main unit loaded with the electrode cartridge is also denoted herein ViaDerm.
- the control unit of the apparatus comprises circuitry to control the magnitude, frequency, and/or duration of the electrical energy delivered to the electrodes, so as to control the current flow or spark generation, and thus the width, depth and shape of the one or more formed micro-channels.
- the electrical energy applied by the control unit is at radio frequency (RF).
- the micro-channels formed by the apparatus of the present invention are hydrophilic and typically have a diameter of about 10 to about 100 microns and a depth of about 20 to about 300 microns, thus facilitating the diffusion of antigenic agents through the skin.
- the electrode cartridge comprises a plurality of electrodes thus forming an electrode array, which generates upon application of an electrical energy a plurality of micro-channels within the subject's skin.
- the overall area of micro-channels generated in the stratum corneum is small compared to the total area covered by the electrode array.
- the term "plurality” refers herein to two or more elements, e.g., two or more electrodes or two or more micro-channels.
- the pressure obtained while placing the apparatus of the present invention on a subject's skin activates the electrical energy delivered to the electrodes.
- Such mode of action ensures that activation of electrodes occurs only in a close contact with the skin enabling the desired formation of the micro- channels.
- the number and dimension of micro-channels may be adjusted to the amount of the antigenic agent desired to be delivered into the skin.
- the electrode cartridge is preferably removable. According to certain embodiments, the electrode cartridge is discarded after one use, and as such is designed for easy attachment to the main unit and subsequent detachment from the main unit.
- the electrodes are preferably shaped and/or supported in a cartridge that is conducive to facilitate formation of micro-channels in the stratum corneum to the desired depth, but not beyond that depth.
- the current can be configured so as to form micro- channels in the stratum corneum without the generation of sparks. The resulted micro- channels are uniform in shape and size.
- the electrodes can be maintained either in contact with the skin, or in vicinity of the skin, up to a distance of about 500 microns therefrom.
- ablation of the stratum corneum is performed by applying electrical current having a frequency between about 10 kHz and about 4000 kHz, preferably between about 10 kHz and about 500 kHz, and more preferably at 100 kHz.
- the present invention further provides a method for inducing an antigen-specific immune response using a transdermal delivery system of the invention.
- the procedure for inducing an antigen-specific immune response comprises a step of placing over the skin the apparatus for generating at least one micro-channel.
- the treatment sites will be swabbed with pads comprising sterile alcohol.
- the site should be allowed to dry before treatment.
- the apparatus containing the electrode array is placed over the site of treatment, the array is energized by RF energy, and treatment is initiated.
- the ablation and generation of micro-channels is completed within seconds.
- the apparatus is removed after micro-channels are generated at limited depth.
- a composition according to the invention is applied to the area of the treated skin where micro-channels are present.
- the present invention thus provides a method for inducing an antigen-specific immune response by transdermal delivery system comprising the steps of: generating at least one micro-channel in an area of the skin of a subject, and applying a composition comprising an immunogenically effective amount of an antigenic agent to the area of skin in which the at least one micro-channel is present, thereby inducing an antigen- specific immune response.
- transdermal delivery refers to delivery of an antigenic agent into or through the dermal layers of the skin, i.e., the epidermis or dermis, beneath the stratum corneum, or into or through the subcutaneous layers of the skin.
- an antigen can be delivered into the skin or through the skin into the blood or lymphatic system.
- transdermal is therefore meant to include also transcutaneous delivery.
- immunogenically effective amount is meant to describe the amount of an antigenic agent, which induces an antigen-specific immune response.
- the immune response induced by the composition of the present invention can comprise humoral (i.e., antigen-specific antibody such as IgM, IgD, IgAl, IgA2, IgE,
- humoral i.e., antigen-specific antibody such as IgM, IgD, IgAl, IgA2, IgE,
- the immune response may comprise NK cells that mediate antibody-dependent cell-mediated cytotoxicity (ADCC).
- the antibody isotypes e.g., IgM, IgD, IgAl, IgA2, IgE, IgGl, IgG2 5 IgG3, and IgG4 can be detected by immunoassay techniques as known in the art (see also the Examples herein below) and/or by a neutralizing assay.
- inducing an immune response is meant to describe the induction of an immune response, whether humoral or cellular, and are used interchangeably throughout the specification and claims of the present invention.
- a neutralization assay for example in a viral neutralization assay, serial dilutions of sera are added to host cells, which are then observed for infection after challenge with infectious virus.
- serial dilutions of sera can be incubated with infectious titers of virus prior to inoculation of an animal, and the inoculated animals are then observed for signs of infection.
- the transdermal immunization system of the invention can be evaluated using challenge models in either animals or humans, which evaluate the ability of immunization with an antigenic agent to protect the subject from a disease. Such protection would demonstrate an antigen-specific immune response.
- induction of an immune response is useful for treating a condition or disease in a subject.
- induction of an immune response by the systems and methods of the present invention provides immunoprotection, immunosuppression, modulation of an autoimmune disease, potentiation of cancer immunosurveillance, prophylactic vaccination to prevent disease, and/or therapeutic vaccination to treat or reduce the severity and/or duration of established disease.
- the antigen is derived from a pathogen, for example, the treatment may vaccinate the subject against infection by the pathogen or against its pathogenic effects such as those caused by toxin secretion.
- a method inherently induces an immune response when it causes a statistically significant change in the magnitude or kinetics of the immune response, change in the induced elements of the immune system (e.g., humoral and/or cellular), effect on the number and/or the severity of disease symptoms, effect on the health and well-being of the subject (i.e., morbidity and mortality), or combinations thereof.
- change in the induced elements of the immune system e.g., humoral and/or cellular
- effect on the number and/or the severity of disease symptoms i.e., morbidity and mortality
- the application site can be protected with anti- inflammatory corticosteroids or non-steroidal anti-inflammatory drugs (NSAIDs) to reduce possible local skin reaction or modulate the type of immune response.
- anti-inflammatory steroids or NSAIDs can be included in the patch material, in creams, ointments, and a like or alternatively corticosteroids or NSAIDs may be applied after application of the formulation of the invention.
- IL-IO, TNF- ⁇ , or any other immunomodulator can be used instead of the anti-inflammatory agents.
- pimecrolimus, tacrolimus, aloevera or any other agent known in the art to reduce local skin reaction can be applied to the treated skin area or included in the patch.
- Vaccination has also been used as a treatment for cancer and autoimmune diseases.
- a tumor antigen e.g., prostate specific antigen
- a tumor antigen can induce an immune response in the form of antibodies, CTLs and lymphocyte proliferation, which allows the body's immune system to recognize and kill tumor cells.
- Tumor antigens useful for vaccination have been described for melanoma, prostate carcinoma, and lymphoma.
- Vaccination with T-cell receptor oligopeptide can induce an immune response that halts the progression of autoimmune disease.
- U.S. Pat. No. 5,552,300 describes antigens suitable for treating autoimmune disease.
- transdermal immunization may be followed with enteral, mucosal, and/or other parenteral techniques for boosting immunization with the same or altered antigens.
- Immunization by an enteral, mucosal, and/or other parenteral route may be followed with transdermal immunization for boosting immunization with the same or altered antigens.
- Transdermal vaccination using an apparatus that generates micro-channels in the skin of a subject was compared to the widely used subcutaneous (SC) and intramuscular (IM) vaccination routes in order to establish its usefulness as a potential vaccine administration system.
- SC subcutaneous
- IM intramuscular
- Ovalbumin (OA) and TIV) were used as exemplary antigens to establish the efficacy of the system of the present invention to induce antigen-specific immune response.
- ovalbumin 50 ⁇ g/ml water; Sigma was used for IM and SC injections.
- a solution of ovalbumin (10 mg/ml) was used for solution transdermal administration (VD-s).
- Ovalbumin powder (2 mg) was used for powder transdermal administration (VD- p).
- a solution pouch was prepared as follows: a 300 ⁇ m thick layer of adhesive (Durotac 2516, National starch, Netherlands) was evenly spread over a silicone sheet (Sil-k Degania Silicone, Israel). The sheet was cut into 4X4cm squares. A square hole (1.57X1.57cm) was cut in the middle of each of the 4X4 squares. A piece of Sil-k silicone 2X2cm is adhered to the 4X4cm silicone square over the 1.57X1.57cm hole using 7701 primer and 4011 glue (Loctite, Ireland). The final product was a pouch of 250 ⁇ l volume.
- Powder patch was prepared as follows: ovalbumin powder was distributed on the skin and then covered with a fixing patch containing BLF 2080 liner (Dow, USA) covered with a layer of Durotak 2516 adhesive (National starch, Netherlands) or alternatively with TegadermTM (3M). Procedure
- Guinea pigs, males, 600-650gr, Dunkin Hartley (7 animals) were anesthetized and blood (1.3 ml) was collected immediately prior to immunization. Ovalbumin solution was then injected (5 ⁇ g; 0.1ml of 50 ⁇ g/ml) to the Quadriceps muscle of the right hind leg. Blood was drawn from each animal at days 8, 15, 22, and 30 after immunization. At day 30, the animals were injected again to the Quadriceps muscle of the right hind leg (boost-5 ⁇ g; 0.1ml of 50 ⁇ g/ml). Blood was collected at days 36, 42, 50, and 125 days after immunization.
- Guinea pigs, males, 600-650gr, Dunkin Hartley (7 animals) were anesthetized and blood (1.3 ml) was collected immediately prior to immunization. Ovalbumin solution was then injected (5 ⁇ g; 0.1ml of 50 ⁇ g/ml) subcutaneously to the dorsal neck area. Blood was drawn from each animal at days 8, 15, 22, and 30 after immunization. At day 30, the animals were injected again (boost-5 ⁇ g; 0.1ml of 50 ⁇ g/ml) subcutaneously to the dorsal neck area. Blood was collected at days 36, 42, 50, and 125 days after immunization.
- Group 3 Transdermal immunization by application of an ovalbumin solution pouch to ViaDerm treated skin
- Guinea pigs, males, 600-650gr, Dunkin Hartley (7 animals) were anesthesized and blood (1.3 ml) was collected immediately prior to immunization.
- the animals were treated with a device, denoted herein ViaDerm, which utilizes electrical energy at radio frequency and consists of an array of electrodes, to generate micro-channels in the skin of the guinea pigs (see, for example, WO 2004/039426; WO 2004/039427; and WO 2004/039428 incorporated by reference as if fully set forth herein).
- Ovalbumin solution pouch (2 mg; 0.2ml of 10 mg/ml) was placed on the treated skin area. Twenty-four hours post application, the pouch was removed. Blood was drawn from each animal at days 8, 15, 22, and 30 after immunization.
- the animals were immunized again by ViaDerm treatment as described above, i.e., burst length ( ⁇ sec) - 700; starting amplitude - 330V; number of bursts - 5; 2 applications on the same skin area (200 pores/cm 2 ), followed by transdermal application of an ovalbumin solution pouch (2 mg; 0.2ml of 10 mg/ml). Blood was collected at days 36, 42, 50, and 125 days after immunization.
- Group 4 Transdermal immunization by application of an ovalbumin powder patch to ViaDerm pretreated skin
- Guinea pigs, males, 600-650gr, Dunkin Hartley (7 animals) were anesthesized and blood (1.3 ml) was collected immediately prior to the immunization.
- the animals were treated with ViaDerm.
- ViaDerm Operating Parameters burst length ( ⁇ sec) - 700; starting amplitude - 330V; number of bursts - 5; 2 applications on the same skin area (200 pores/cm 2 ).
- Ovalbumin powder (2 mg) was evenly distributed with a spatula on the treated skin area and then covered with a fixing patch. Twenty-four hours post application, the patch was removed. Blood was drawn from each animal at days 8, 15, 22, and 30 after immunization.
- the animals were immunized again by ViaDerm treatment as described above, i.e., burst length ( ⁇ sec) - 700; starting amplitude - 330V; number of bursts - 5; 2 applications on the same skin area (200 pores/cm 2 ), followed by transdermal application of ovalbumin powder (2 mg; 0.2ml of 10 mg/ml) as described above. Blood was collected at days 36, 42, 50, and 125 days after immunization.
- Guinea pig's plasma samples serially diluted with the diluent/blocker, were added to the ovalbumin-coated plates in triplicates and incubated for one hour at 22 0 C. Unbound antibodies were washed three times with the wash solution.
- the wells were incubated for one hour at 22 0 C with horseradish-peroxidase (HRP) conjugated goat-anti guinea pig IgG antibody diluted in the diluent/blocker solution (Jackson Immunoresearch Laboratories, 0.8mg/ml, 1 : 10,000), and then washed three times with the wash solution.
- HRP horseradish-peroxidase
- Table 2 TEWL of boost immunization.
- IgM antibodies 15 days after primary immunization represent the earliest response to antigen presentation.
- the group of animals injected subcutaneously (SC) with ovalbumin and the group of animals treated with ViaDerm and thereafter immunized against ovalbumin by the ovalbumin solution pouch (VD-s) showed induction of ovalbumin specific IgM antibodies, though the IgM antibody titer detected in the SC group was higher than in the VD-s group.
- both groups demonstrated similar incidence of "non-responder" animals, e.g., animals that did not show detectable titer of IgM antibodies.
- FIG. 2 presents the IgG plasma titers 15 days post immunization. There was a significant difference between the VD-s group and the SC group. As shown in FIG. 2, generation of micro-channels by ViaDerm treatment and subsequent application of the ovalbumin solution pouch (VD-s) resulted in significantly higher IgG titers at day 15 compared to the titers obtained by SC injection. These results clearly indicate that ViaDerm treatment can shorten the time for IgG antibodies appearance. This effect is highly advantageous as IgG antibodies are the most important antibody subtype in an antigen specific immune response.
- FIG. 2 presents the IgG plasma titers 15 days post immunization. There was a significant difference between the VD-s group and the SC group. As shown in FIG. 2, generation of micro-channels by ViaDerm treatment and subsequent application of the ovalbumin solution pouch (VD-s) resulted in significantly higher IgG titers at day 15 compared to the titers obtained by SC
- FIG. 2 also shows that all the animals in the VD-s and SC groups were found to be positive for antigen specific plasma IgG antibodies.
- FIG. 2 further shows that there was low variability between the individual animals.
- the single animal of the VD-s group that did not show detectable titer of IgG (Animal No. 19) was found to be in a bad physical condition at the time of bleeding and died the next day. Animal No. 19 did not have any detectable antigen specific IgM and IgA.
- the antigen specific plasma IgA titer was determined in the SC and the VD-s groups at 15 days post primary antigen presentation (FIG. 5). Only 2 out of 7 animals in the SC group demonstrated detectable IgA titers compared to 4 animals out of 6 in the VD-s group. This superiority of the VD-s treatment compared to the SC injection was further demonstrated six days after boost administration (FIG. 6). The animals that had no detectable specific IgA response after boost administration (animals Nos. 9 & 11) had neither IgA nor IgM at 15 days post immunization. All animals (SC and VD-s) were IgA positive 12 days after antigen boosting (FIG. 6).
- the significant immune response following VD antigen presentation included all the important plasma antibody isotypes: IgM, IgG and IgA, thus indicating efficient isotype switching.
- IgG and IgM antibody titers There was no correlation between IgG and IgM antibody titers in the VD-s vs. S. C. groups.
- IgG titers were observed in the VD-s group vs. SC group
- higher IgM titers were observed in the SC group vs. VD-s group during the primary response. Without being bound to any theory, this phenomenon may be explained by a very efficient cellular response, which takes place following VD application.
- VD treatment was used with two ovalbumin formulations, i.e., powder (VD-p) and solution (VD-s), at the same dose and in the absence of any adjuvant.
- VD-p ovalbumin formulations
- VD-s solution formulations
- the lower IgG titer in the VD-p vs. VD-s emphasized that antigen-formulation is critical for successful vaccine development.
- the impressive IgA titer in the VD-p group compared to the poor IgG titer strongly indicates that antigen formulation can play a significant role in manipulating the immune response as desired.
- transdermal immunization using ViaDerm technology is highly efficient and can provide an alternative technique for the traditional vaccination routes.
- EXAMPLE 2 Transdermal immunization with trivalent influenza vaccine
- Inactivated influenza vaccine A/Panama/2007/99, A/New Caledonia/20/99 and
- LT heat labile enterotoxin
- ViaDerm Length of electrodes 50 and 100 ⁇ m, cylinder shape.
- Adhesive tape 3 M
- the guinea pigs were shaved and sedated with ketamine and xylazine. All animals were bolus intramuscular injected with 0.5 ⁇ g HA (0.17 ⁇ g HA each strain) in lOOul IxDPBS on study day 1.
- a 1 cm rayon patch containing 15 ⁇ g HA (5 ⁇ g HA each strain) alone (no LT) or with 1 ⁇ g LT in 15 ⁇ l IxDPBS were applied immediately after the pretreatment. To insure proper patch adherence, patches were covered with a modified Tegaderm overlay. The patch was wrapped with adhesive tape. Patches were applied for 18-24 hr, removed, and the skin was rinsed with warm water.
- Pre-immune (prior to immunization) and post immune (day 22 and 36) blood samples were collected from the orbital plexus using standard methods. Serum was collected by centrifugation of whole blood and the cell free serum transferred to a labeled tube and stored frozen at -2O 0 C.
- Sera was evaluated for total IgG titers to A/Panama, A/New Caledonia, and
- Antibody titers were presented as ELISA Units (EU), which is the serum dilution equal to 1 O.D. at 405 nm.
- EU ELISA Units
- FIG. 7 shows the TEWL values of non-treated or ViaDerm treated guinea pigs.
- FIG. 8 shows serum IgG antibody titers against A/Panama influenza strain in the absence or presence of E. coli heat labile enterotoxin (LT) as an adjuvant in guinea pigs treated with ViaDerm and immunized by a patch containing the trivalent influenza vaccine.
- LT heat labile enterotoxin
- guinea pigs were immunized intramuscularly (IM) with 0.5 ⁇ g of the trivalent influenza vaccine at day 1, and boosted IM with the same vaccine (15 ⁇ g) at day 22. As shown in FIG.
- FIGs. 9 and 10 show similar results when serum IgG antibody titers against
- A/New Caledonia strain and B/Shangdong strain of influenza were determined. As shown in FIGs. 9 and 10, the IgG antibody titers against each of these strains was significantly higher in the ViaDerm treated guinea pigs that were then administered with the influenza patch as compared to guinea pigs not treated with ViaDerm but administered with the influenza patch. Addition of LT as an adjuvant did not improve the IgG antibody titers. The IgG antibody titers in ViaDerm treated animals were comparable to those obtained in guinea pigs injected intramuscularly with the trivalent influenza vaccine.
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/571,460 US20070292445A1 (en) | 2004-07-06 | 2005-07-05 | Delivery system for transdermal immunization |
EP05758964A EP1768742A4 (en) | 2004-07-06 | 2005-07-05 | Delivery system for transdermal immunization |
CA002572870A CA2572870A1 (en) | 2004-07-06 | 2005-07-05 | Delivery system for transdermal immunization |
JP2007519978A JP2008505685A (en) | 2004-07-06 | 2005-07-05 | Transdermal immunization delivery system |
US11/324,007 US20070009542A1 (en) | 2005-07-05 | 2005-12-30 | Method and device for transdermal immunization |
IL180509A IL180509A (en) | 2004-07-06 | 2007-01-02 | Delivery system for transdermal immunization |
Applications Claiming Priority (2)
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US58508104P | 2004-07-06 | 2004-07-06 | |
US60/585,081 | 2004-07-06 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
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US11/324,007 Continuation-In-Part US20070009542A1 (en) | 2005-07-05 | 2005-12-30 | Method and device for transdermal immunization |
Publications (2)
Publication Number | Publication Date |
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WO2006003659A2 true WO2006003659A2 (en) | 2006-01-12 |
WO2006003659A3 WO2006003659A3 (en) | 2006-04-20 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/IL2005/000710 WO2006003659A2 (en) | 2004-07-06 | 2005-07-05 | Delivery system for transdermal immunization |
Country Status (5)
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US (1) | US20070292445A1 (en) |
EP (1) | EP1768742A4 (en) |
JP (1) | JP2008505685A (en) |
CA (1) | CA2572870A1 (en) |
WO (1) | WO2006003659A2 (en) |
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WO2010100650A2 (en) | 2009-03-04 | 2010-09-10 | Regenera Pharma Ltd. | Therapeutic uses of mastic gum fractions |
WO2007077487A3 (en) * | 2005-12-30 | 2010-11-11 | Transpharma Medical, Ltd. | Method and device for transdermal immunization |
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- 2005-07-05 CA CA002572870A patent/CA2572870A1/en not_active Abandoned
- 2005-07-05 JP JP2007519978A patent/JP2008505685A/en active Pending
- 2005-07-05 US US11/571,460 patent/US20070292445A1/en not_active Abandoned
- 2005-07-05 WO PCT/IL2005/000710 patent/WO2006003659A2/en active Application Filing
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Also Published As
Publication number | Publication date |
---|---|
JP2008505685A (en) | 2008-02-28 |
CA2572870A1 (en) | 2006-01-12 |
US20070292445A1 (en) | 2007-12-20 |
WO2006003659A3 (en) | 2006-04-20 |
EP1768742A4 (en) | 2007-10-17 |
EP1768742A2 (en) | 2007-04-04 |
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