WO2006002221A3 - Fluorogenic enzyme assays and substrates for kinases and phosphatases - Google Patents

Fluorogenic enzyme assays and substrates for kinases and phosphatases Download PDF

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Publication number
WO2006002221A3
WO2006002221A3 PCT/US2005/022030 US2005022030W WO2006002221A3 WO 2006002221 A3 WO2006002221 A3 WO 2006002221A3 US 2005022030 W US2005022030 W US 2005022030W WO 2006002221 A3 WO2006002221 A3 WO 2006002221A3
Authority
WO
WIPO (PCT)
Prior art keywords
phosphatases
kinases
substrates
enzyme assays
fluorogenic enzyme
Prior art date
Application number
PCT/US2005/022030
Other languages
French (fr)
Other versions
WO2006002221A2 (en
Inventor
Linda G Lee
Hongye Sun
Original Assignee
Applera Corp
Linda G Lee
Hongye Sun
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp, Linda G Lee, Hongye Sun filed Critical Applera Corp
Priority to EP05762562A priority Critical patent/EP1759015A2/en
Publication of WO2006002221A2 publication Critical patent/WO2006002221A2/en
Publication of WO2006002221A3 publication Critical patent/WO2006002221A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase

Abstract

The invention claims a substrate molecule comprising a hydrophobic moiety capable of integrating the substrate molecule into a micelle, a substrate moiety, comprising two or more enzyme recognition moieties, wherein each recognition moiety, independently of the other, comprises one or more residues capable of being phosphorylated or dephosphorylated, and at least one fluorescent moiety.
PCT/US2005/022030 2004-06-21 2005-06-21 Fluorogenic enzyme assays and substrates for kinases and phosphatases WO2006002221A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05762562A EP1759015A2 (en) 2004-06-21 2005-06-21 Fluorogenic enzyme assays and substrates for kinases and phosphatases

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US58203804P 2004-06-21 2004-06-21
US60/582,038 2004-06-21
US62336304P 2004-10-29 2004-10-29
US60/623,363 2004-10-29

Publications (2)

Publication Number Publication Date
WO2006002221A2 WO2006002221A2 (en) 2006-01-05
WO2006002221A3 true WO2006002221A3 (en) 2006-05-04

Family

ID=35482300

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/022030 WO2006002221A2 (en) 2004-06-21 2005-06-21 Fluorogenic enzyme assays and substrates for kinases and phosphatases

Country Status (2)

Country Link
EP (1) EP1759015A2 (en)
WO (1) WO2006002221A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1704257A2 (en) 2004-01-16 2006-09-27 Applera Corporation Fluorogenic kinase assays and substrates for kinases and phosphatases
EP1841881A2 (en) * 2004-12-30 2007-10-10 Applera Corporation Compositions, methods and kits for real-time enzyme assays using charged molecules

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001007638A2 (en) * 1999-07-27 2001-02-01 University Of Utah Research Foundation Homogeneous fluorescence method for assaying structural modifications of biomolecules
US20030135869A1 (en) * 2000-10-10 2003-07-17 Steven Farber High throughput genetic screening of lipid and cholesterol processing using fluorescent compounds
WO2004022773A1 (en) * 2002-09-09 2004-03-18 Applera Corporation Fluorescent enzyme assay methods and compositions
WO2005054495A2 (en) * 2003-11-26 2005-06-16 Applera Corporation Ligand-containing micelles and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001007638A2 (en) * 1999-07-27 2001-02-01 University Of Utah Research Foundation Homogeneous fluorescence method for assaying structural modifications of biomolecules
US20030135869A1 (en) * 2000-10-10 2003-07-17 Steven Farber High throughput genetic screening of lipid and cholesterol processing using fluorescent compounds
WO2004022773A1 (en) * 2002-09-09 2004-03-18 Applera Corporation Fluorescent enzyme assay methods and compositions
WO2005054495A2 (en) * 2003-11-26 2005-06-16 Applera Corporation Ligand-containing micelles and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HENDRICKSON H STEWART: "Fluorescence-based assays of lipases, phospholipases, and other lipolytic enzymes", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 219, no. 1, 1994, pages 1 - 8, XP002308357, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
WO2006002221A2 (en) 2006-01-05
EP1759015A2 (en) 2007-03-07

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