WO2005109995A3 - Thermostable gluconate dehydratase and use thereof - Google Patents

Thermostable gluconate dehydratase and use thereof Download PDF

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Publication number
WO2005109995A3
WO2005109995A3 PCT/KR2004/001126 KR2004001126W WO2005109995A3 WO 2005109995 A3 WO2005109995 A3 WO 2005109995A3 KR 2004001126 W KR2004001126 W KR 2004001126W WO 2005109995 A3 WO2005109995 A3 WO 2005109995A3
Authority
WO
WIPO (PCT)
Prior art keywords
gluconate dehydratase
daltons
native
dehydratase
thermostable
Prior art date
Application number
PCT/KR2004/001126
Other languages
French (fr)
Other versions
WO2005109995A2 (en
Inventor
Seonghun Kim
Sun Bok Lee
Original Assignee
Posco
Postech Foundation
Seonghun Kim
Sun Bok Lee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Posco, Postech Foundation, Seonghun Kim, Sun Bok Lee filed Critical Posco
Priority to PCT/KR2004/001126 priority Critical patent/WO2005109995A2/en
Priority to US11/568,848 priority patent/US20090239249A1/en
Priority to KR1020087017906A priority patent/KR100967716B1/en
Priority to KR1020097015700A priority patent/KR100972204B1/en
Publication of WO2005109995A2 publication Critical patent/WO2005109995A2/en
Publication of WO2005109995A3 publication Critical patent/WO2005109995A3/en
Priority to US12/910,768 priority patent/US8383375B2/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01039—Gluconate dehydratase (4.2.1.39)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a novel thermostable gluconate dehydratase from the thermoacidophilic archaeon Sulfolobus solfataricus, a coding sequence, and an expression system. The gluconate dehydratase has a molecular weight of about 320,000 to 380,000 daltons as the native protein, and about 40,000 to 50,000 daltons as the monomer protein, and catalyzes the dehydration reaction of aldonic acids to 2-keto-3-deoxy derivatives at temperatures of less than 120 °C. The gluconate dehydratase can be produced from native or recombinant host cells and thereby used in the pharmaceutical, agricultural, and other industries.
PCT/KR2004/001126 2004-05-13 2004-05-13 Thermostable gluconate dehydratase and use thereof WO2005109995A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
PCT/KR2004/001126 WO2005109995A2 (en) 2004-05-13 2004-05-13 Thermostable gluconate dehydratase and use thereof
US11/568,848 US20090239249A1 (en) 2004-05-13 2004-05-13 Novel thermostable gluconate dehydratase and use thereof
KR1020087017906A KR100967716B1 (en) 2004-05-13 2004-05-13 Novel thermostable gluconate dehydratase and use thereof
KR1020097015700A KR100972204B1 (en) 2004-05-13 2004-05-13 A method of purifying a gluconate dehydratase from archaea
US12/910,768 US8383375B2 (en) 2004-05-13 2010-10-22 Thermostable gluconate dehydratase and use thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR2004/001126 WO2005109995A2 (en) 2004-05-13 2004-05-13 Thermostable gluconate dehydratase and use thereof

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US11/568,848 A-371-Of-International US20090239249A1 (en) 2004-05-13 2004-05-13 Novel thermostable gluconate dehydratase and use thereof
US12/910,768 Division US8383375B2 (en) 2004-05-13 2010-10-22 Thermostable gluconate dehydratase and use thereof

Publications (2)

Publication Number Publication Date
WO2005109995A2 WO2005109995A2 (en) 2005-11-24
WO2005109995A3 true WO2005109995A3 (en) 2006-06-01

Family

ID=35394570

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2004/001126 WO2005109995A2 (en) 2004-05-13 2004-05-13 Thermostable gluconate dehydratase and use thereof

Country Status (3)

Country Link
US (2) US20090239249A1 (en)
KR (2) KR100967716B1 (en)
WO (1) WO2005109995A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5161588B2 (en) * 2005-03-04 2013-03-13 カーネギー インスチチューション オブ ワシントン Environmentally stable sensor and method of using the same
CA2737112A1 (en) * 2008-10-27 2010-06-03 Butamax Advanced Biofuels Llc Carbon pathway optimized production hosts for the production of isobutanol
TWI406944B (en) * 2011-06-23 2013-09-01 Univ Ming Chi Technology Glucose dehydrogenase and blood sugar measurement using thereof
BR112020023407A2 (en) 2018-05-17 2021-02-09 Bp Corporation North America Inc. production of 2-keto-3-deoxy-d-gluconic acid in filamentous fungi

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0697461A1 (en) * 1994-07-21 1996-02-21 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Recombinant thermostable enzyme which releases trehalose from non-reducing saccharide

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0697461A1 (en) * 1994-07-21 1996-02-21 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Recombinant thermostable enzyme which releases trehalose from non-reducing saccharide

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LAMBLE H.J. ET AL: "Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 36, 2003, pages 34066 - 34072, XP002531754, DOI: doi:10.1074/JBC.M305818200 *
SHE Q. ET AL: "The complete genome of the crenarchaeon Sulfolobus solfataricus P2", PROC. NATL. ACAD SCI. USA, vol. 98, no. 14, 2001, pages 7835 - 7840, XP002625002, DOI: doi:10.1073/PNAS.141222098 *

Also Published As

Publication number Publication date
WO2005109995A2 (en) 2005-11-24
KR100972204B1 (en) 2010-07-23
US20110111471A1 (en) 2011-05-12
KR20080072104A (en) 2008-08-05
KR20090086136A (en) 2009-08-10
US20090239249A1 (en) 2009-09-24
KR100967716B1 (en) 2010-07-07
US8383375B2 (en) 2013-02-26

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