WO2005106476B1 - Method for diagnosing infectious diseases - Google Patents
Method for diagnosing infectious diseasesInfo
- Publication number
- WO2005106476B1 WO2005106476B1 PCT/US2005/013446 US2005013446W WO2005106476B1 WO 2005106476 B1 WO2005106476 B1 WO 2005106476B1 US 2005013446 W US2005013446 W US 2005013446W WO 2005106476 B1 WO2005106476 B1 WO 2005106476B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- test sample
- level
- antibody binding
- animal
- microorganism
- Prior art date
Links
- 201000009910 diseases by infectious agent Diseases 0.000 title claims abstract 9
- 244000005700 microbiome Species 0.000 claims abstract 22
- 102000004965 antibodies Human genes 0.000 claims abstract 16
- 108090001123 antibodies Proteins 0.000 claims abstract 16
- 241000187482 Mycobacterium avium subsp. paratuberculosis Species 0.000 claims 9
- 108010032595 Antibody Binding Sites Proteins 0.000 claims 6
- 241000186359 Mycobacterium Species 0.000 claims 6
- 210000002966 Serum Anatomy 0.000 claims 5
- 238000001514 detection method Methods 0.000 claims 5
- 241000124008 Mammalia Species 0.000 claims 4
- 241000283690 Bos taurus Species 0.000 claims 3
- 241000186367 Mycobacterium avium Species 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 2
- 238000011179 visual inspection Methods 0.000 claims 2
- 201000010099 disease Diseases 0.000 claims 1
- 238000000684 flow cytometry Methods 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
Abstract
A method for diagnosing the presence in an animal of an infection due to a microorganism in which a test sample obtained from the animal is exposed to a population of the microorganism and the presence of binding of the microorganism by an antibody in the test sample is determined.
Claims
1. A method for diagnosing the presence in an animal of an infection caused by a microorganism comprising obtaining a test sample from the animal, exposing the test sample to a population of the microorganism for a time sufficient to permit an antibody in the test sample that binds to an antibody binding site of the microorganism to bind to the antibody binding site, putting the microorganisms that were exposed to the test sample in suspension, determining the level of binding of the suspended microorganisms to antibodies in the test sample and comparing the level of the antibody binding in the test sample with the level of antibody binding from a negative control, wherein the presence of infection is diagnosed if the level of antibody binding in the test sample is significantly higher than that in the control, and wherein the microorganism is a mycobacterium.
2. The method of claim 1 wherein the animal is a mammal.
3. The method of claim 2 wherein the mammal is a bovine. 33
4. The method of claim 1 wherein the mycobacterium is Mycobacterium avium.
5. The method of claim 4 wherein the mycobacterium is Mycobacterium avium subsp. paratuberculosis.
6. The method of claim 1 wherein the test sample is serum.
7. The method of claim 6 wherein the serum test sample is obtained from the animal by venipuncture.
8. The method of claim 1 wherein the population of the microorganism contains intact whole microorganisms .
9. The method of claim 8 wherein the intact whole microorganisms are killed microorganisms.
10. The method of claim 1 wherein the detection of the level of binding is by flow cytometry.
11. A method for diagnosing infection caused by Mycobacterium avium subsp. paratuberculosis (MAP) in an animal comprising obtaining a test sample from the animal, exposing the test sample to a population of MAP organisms for a time sufficient to permit an antibody in the test sample that binds to an antibody binding site of MAP to bind to the antibody binding site, determining the level of binding of the MAP organisms to antibodies in the test sample, and comparing the level of the antibody binding in the test sample with the level of antibody binding from a negative control, wherein the presence of infection is diagnosed if the level of antibody binding in the test sample is significantly higher than that in the control .
12. The method of claim 11 wherein the infection is Johne's Disease.
13. The method of claim 11 wherein the animal is a bovine.
14. The method of claim 11 wherein the test sample is serum.
15. The method of claim 11 wherein intact whole MAP organisms are exposed to the test sample.
16. The method of claim 15 wherein the intact whole MAP organisms are killed.
AMENDED SHRET (ARTICLE 19) 35
17. The method of claim 11 wherein the detection of the complexes is by visual inspection.
18. The method of claim 17 wherein the detection of the presence of binding is by blot analysis.
19. A method for diagnosing the presence in an animal of an infection caused by a microorganism comprising obtaining a test sample from /the animal, exposing the test sample to a population of the microorganism fixed on a surface for a time sufficient to permit an antibody in the test sample that binds to an antibody binding site of the microorganism to bind to the antibody binding site, and determining the level of binding of the microorganisms on the surface to antibodies in the test sample, and comparing the level of the antibody binding in the test sample with the level of antibody binding from a negative control, wherein the presence of infection is diagnosed if the level of antibody binding in the test sample is significantly higher than that in the control, and wherein the microorganism is a mycobacterium.
20. The method of claim 19 wherein the mycobacterium is Mycobacterium avium. 36
21. The method of claim 20 wherein the mycobacterium is Mycobacterium avium subsp. paratuberculosis.
22. The method of claim 19 wherein the animal is a mammal.
23. The method of claim 22 wherein the mammal is a bovine.
24. The method of claim 19 wherein the test sample is serum.
25. The method of claim 24 wherein the serum test sample is obtained from the animal by venipuncture.
26. The method of claim 19 wherein the population of the microorganism contains intact whole microorganisms.
27. The method of claim 26 wherein the intact whole microorganisms are killed microorganisms.
28. The method of claim 19 wherein the detection of the complexes is by visual inspection. 37
29. The method of claim 28 wherein the detection of the presence of binding is by blot analysis.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05737662A EP1747025B1 (en) | 2004-04-27 | 2005-04-19 | Method for diagnosing infectious diseases |
| DE602005024254T DE602005024254D1 (en) | 2004-04-27 | 2005-04-19 | |
| AT05737662T ATE485060T1 (en) | 2004-04-27 | 2005-04-19 | METHOD FOR DIAGNOSING INFECTIOUS DISEASES |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/832,761 | 2004-04-27 | ||
| US10/832,761 US7276350B2 (en) | 2004-04-27 | 2004-04-27 | Method for diagnosing infectious diseases |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO2005106476A2 WO2005106476A2 (en) | 2005-11-10 |
| WO2005106476A3 WO2005106476A3 (en) | 2005-12-29 |
| WO2005106476B1 true WO2005106476B1 (en) | 2006-04-20 |
Family
ID=35136956
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2005/013446 WO2005106476A2 (en) | 2004-04-27 | 2005-04-19 | Method for diagnosing infectious diseases |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US7276350B2 (en) |
| EP (1) | EP1747025B1 (en) |
| AT (1) | ATE485060T1 (en) |
| DE (1) | DE602005024254D1 (en) |
| WO (1) | WO2005106476A2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102008029834A1 (en) * | 2008-06-25 | 2010-01-14 | Justus-Liebig-Universität Giessen | Method for the specific detection of MAP antibodies |
| US8008033B2 (en) | 2008-10-15 | 2011-08-30 | Gilles Reza Georges Monif | Fuidi herd management schema |
| US9568444B2 (en) | 2012-01-27 | 2017-02-14 | University Of Tennessee Research Foundation | Method and apparatus for detection of a biomarker by alternating current electrokinetics |
| US9696304B2 (en) | 2012-01-27 | 2017-07-04 | University Of Tennessee Research Foundation | Methods for detecting a biomarker by alternating current electrokinetics |
| US9128098B2 (en) | 2012-10-31 | 2015-09-08 | Gilles R. G. Monif | Fuidi herd management and risk stratification methods |
| CA2988297A1 (en) | 2016-12-28 | 2018-06-28 | University Of Tennessee Research Foundation | Methods for detecting a biomarker by alternating current electrokinetics |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL9202197A (en) * | 1992-12-17 | 1994-07-18 | Kreatech Biotech Bv | Method and device for identifying a mycobacterium species responsible for mycobacterial infection. |
-
2004
- 2004-04-27 US US10/832,761 patent/US7276350B2/en active Active
-
2005
- 2005-04-19 WO PCT/US2005/013446 patent/WO2005106476A2/en active Application Filing
- 2005-04-19 EP EP05737662A patent/EP1747025B1/en not_active Not-in-force
- 2005-04-19 AT AT05737662T patent/ATE485060T1/en not_active IP Right Cessation
- 2005-04-19 DE DE602005024254T patent/DE602005024254D1/de active Active
-
2007
- 2007-02-01 US US11/701,202 patent/US7422869B2/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Cox et al. | Development and evaluation of a rapid absorbed enzyme immunoassay test for the diagnosis of Johne's disease in cattle | |
| US7192721B1 (en) | Universal rapid test and method for detection of tuberculosis in multiple host species | |
| EP2985349A1 (en) | Device for detection of analytes | |
| RU2617400C2 (en) | Method for specific substance detection in milk | |
| Kanade et al. | Utility of MPT 64 antigen detection assay for rapid characterization of mycobacteria in a resource constrained setting | |
| WO2005106476B1 (en) | Method for diagnosing infectious diseases | |
| WO2010070581A1 (en) | Method and device for diagnosing tuberculosis | |
| Strain et al. | Bovine tuberculosis: a review of diagnostic tests for M. bovis infection in badgers | |
| JP2009507243A (en) | How to diagnose an infection | |
| EP2005182B1 (en) | Clinical correlates | |
| Vannuffel et al. | Development of species-specific enzyme-linked immunosorbent assay for diagnosis of Johne's disease in cattle | |
| EP1747025B1 (en) | Method for diagnosing infectious diseases | |
| JP2021508836A (en) | Tuberculosis diagnostic method | |
| Gonzalez et al. | Development of a POC test for TB based on multiple immunodominant epitopes of M. tuberculosis specific cell-wall proteins | |
| EP0402993A3 (en) | Diagnostic test kit and method for determination of chlamydial antigen using a membrane having surface hydroxy groups | |
| O'Brien et al. | Evaluation of blood assays for detection of Mycobacterium bovis in white-tailed deer (Odocoileus virginianus) in Michigan | |
| RU2532352C2 (en) | Method of carrying out immunochromatographic analysis for serodiagnostics | |
| EP1565745A2 (en) | Isolation and confirmation of analytes from test devices | |
| JP2010502729A (en) | Assay for antibodies against B. pneumoniae | |
| WO2000017640A1 (en) | Immunoassay for equine protozoal myeloencephalitis in horses | |
| WO2003098212B1 (en) | Method of isolating an endotehelial cell and method of donor specific crossmatching | |
| Sumi et al. | Dot-immunobinding assay | |
| Vatsos et al. | Development of an immunofluorescent antibody technique (IFAT) and in situ hybridization to detect Flavobacterium psychrophilum in water samples. | |
| RU2545909C2 (en) | Method of immunochromatographic determination of specific antibodies | |
| CA2375503A1 (en) | An assay for the determination of newly synthesised antibodies in lymphocytes |