WO2005092122A1

WO2005092122A1 - Composition comprising yucca extract, quillaia extract and lactic acid bacterium and food and drink containing the composition

Info

Publication number: WO2005092122A1
Application number: PCT/JP2005/003013
Authority: WO
Inventors: Yasuhiro Koga; Masaaki Shioya; Yohji Ezure
Original assignee: Nihon Medicine Co., Ltd.; Celltechs Health Co., Ltd.
Priority date: 2004-03-26
Filing date: 2005-02-24
Publication date: 2005-10-06
Also published as: JP2007082403A; JPWO2005092122A1; KR20060135016A

Abstract

A composition whereby blood cholesterol level and blood triglyceride level can be effectively lowered and, at the same time, the effect of a composition comprising a yucca extract and a Quillaia saponaria extract of improving digestive tract function can be synergistically enhanced; and a composition comprising a yucca extract and a Quillaia saponaria extract together with a lactic acid bacterium or a food or a drink containing this composition aiming at providing a food or a drink.

Description

Specification

A composition comprising yucca extract, quilla extract and lactic acid bacteria, and food and drink containing the composition

Technical field

[0001] The present invention relates to a composition obtained by mixing and blending a Yucca extract, a Quillaja extract and a lactic acid bacterium, or a food or drink containing the composition.

Background art

[0002] The predominance of lactic acid bacteria in the flora of intestinal microorganisms enhances the general function of lactic acid bacteria as so-called good bacteria, which is achieved only by lowering blood cholesterol and blood triglycerides. That is, enhancement of lactic acid bacteria's intestinal action, constipation-improving action, various vitamin-producing actions, and suppression of harmful substances (such as harmful amines produced by spoilage bacteria). In this way, preserving the balance of enteric bacteria by predominating good bacteria is important for sustaining youth and health.

[0003] As is well known, lactic acid bacteria are bacteria that proliferate using sugars such as lactose and glucose, and produce a large amount of lactic acid in the process (homo-type lactic acid fermentation bacteria) or lactic acid, alcohol and alcohol. Bacteria that produce carbon dioxide and the like (hetero-type lactic acid fermentation bacteria), and also include bifidobacteria that produce lactic acid and acetic acid.

[0004] Lactic acid bacteria are classified into lactic acid bacteria for fermented milk and intestinal lactic acid bacteria. Lactic acid bacteria for fermented milk are lactic acid nephrite! ¾J¾ (Lactobaculus delbrueckn subsp. , Lactococcus lactis subsp. Cremoris, Lactococcus deacetilactis, etc.) and leuconostoc (Leuconostoc cremoris, etc.).

[0005] Intestinal lactic acid bacteria are Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus amylovorus, Lactobacillus gallinarum, Lactobacillus gasseri,

Lactobacillus johnsonii ^ Lactobacillus casei, Lactobacillus casei subsp.rhamnosus), Lactococcus (Enterococcus faecium, etc.) and Bifidobacterium bifidum (Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve ^ Bifidobacterium infantis,

Bifidobacterium adolescentis).

[0006] Lactic acid bacteria include spore-forming lactic acid bacteria such as Bacillus coagulans and Lactobacillus sporogenes. In addition, Bulgarian, Thermophilus, Acidophilus, and Bifidobacterium used in the production of yogurt are also included in lactic acid bacteria.

[0007] A blood cholesterol lowering action and a method for reducing cholesterol in foods have been proposed using the function of lactic acid bacteria. For example, foods and drinks using lactic acid bacteria belonging to Ratatococcus (see JP-A-5-65229), compositions using freeze-dried lactic acid bacteria (see JP-A-6-56680), novel lactobacilli that do not exhibit bile acid deconjugation A method using lactic acid bacteria (see JP-A-7-250670), fermented soymilk by lactic acid bacteria (see JP-A-10-229841), mold culture, fermenter, composition containing lactic acid bacteria culture and food (see JP-A-10-298083) ), A method using a conjugated bile acid deconjugating enzyme produced by lactic acid bacteria belonging to the genus Enterococcus (see JP 2000-166548), a lactobacillus lactic acid bacteria whey protein culture and Streptococcus thermophilus (Streptococcus food using a mixture of whey protein cultures of Thermophilus (see JP 2000-197469), Lactococcus lactis subsp. lactis Method for reducing cholesterol in foods used (see JP 2002-65203), fermented soy milk by lactic acid bacteria (see JP 2003-81855), ratatobacillus 'plantarum (Lactobacillus plantarum), lactobacillus' Ligobacillus hilgardii , Food using Lactobacillus paralimentarius (see JP 2003-235501), Food and drink using Lactobacillus gasseri (see JP 2003-306436), Spores (See JC Mohan et al., Indian J. Med. Res., Vol. 95, pp.431-432, 1990).

[0008] On the other hand, many basic studies and clinical trials have been conducted on the blood cholesterol lowering effect and blood triglyceride lowering action of lactic acid bacteria. In particular, a review centered on the results of 11 human clinical trials conducted since 1990 and reported as an academic paper was published (see Xiao Jinchu, History of Medicine, 207, 835-840, 2003). [0009] However, in the comprehensive judgment on these 11 academic papers, it is concluded that lactic acid bacteria have both a result of lowering blood cholesterol and a result of not reducing it, and neither can be declared. there were. In any case, it can be said that lactic acid bacteria are not satisfactory because the blood cholesterol lowering effect is insufficient.

[0010] In all of these 11 reports, the results of lowering blood triglycerides were unsatisfactory. In other words, it can be concluded that lactic acid bacteria are ineffective in reducing blood triglycerides.

[0011] By the way, the yucca extract is an extract obtained by extracting with water or hydrous alcohol from the rhizome of Yucca schidigera of the lily family. Quillaja extract is an extract obtained by extracting water or hydrous alcohol from the husk of Quillaja saponalia of the Rosaceae family.

[0012] Yucca extract and quilla extract are approved as food additives derived from natural products in Japan, the United States, and the like, and are widely used as emulsifiers and foaming agents for beverages and cosmetics. The yucca extract and quilla extract have unique aromas, tastes and colors, and can be used as food materials as they are, but preferably further purified products, and in some cases highly purified products are desired.

[0013] With regard to yucca extract and quilla extract, each single extract has been reported to have a blood cholesterol lowering effect (J. Milgate and DCK Roberts, Nutrition Research, Vol. 15, No.8, pp.1223—1249, 1995).

[0014] Yarn and quilla extracts are synergistic with human blood total cholesterol lowering effect, human blood LDL cholesterol lowering effect and gastrointestinal function improving action. The effectiveness of the yucca extract alone and kiraja extract alone, and the mixing ratio of yucca extract and kiraja extract is 96: 4-65: An example of various changes in the range of 35, and a typical example of a mixing ratio having an excellent human blood cholesterol lowering effect is that the extract of quilla extract is ¾0% and the yucca extract is 40%. Have already been disclosed (see PCT / KR01 / 00299 and SW Kim et al, Arch. Pharm. Res., Vol. 26, No. 12, pp. 1042-1046, 2003). In addition, as a form of food or drink containing a composition of yucca and quilla extract, it is a solution-like drink. Forms and solid food forms are known. (See PCT / KR01 / 00299 and Japanese Patent Application 2003-394738). However, according to these documents, the blood triglyceride-lowering effect of the composition comprising Yucca and Quillaja extract is recognized!

Disclosure of the invention

Problems to be solved by the invention

[0015] As described above, the blood cholesterol lowering action of conventional functional foods and drinks using lactic acid bacteria is not strong but is insufficient, and the blood triglyceride lowering action of lactic acid bacteria has been found so far. It has been unpowered. In order to increase the functionality of these lactic acid bacteria, it has the function of accelerating the growth of lactic acid bacteria, increasing the blood cholesterol lowering effect of lactic acid bacteria, and lowering blood triglycerides. Development of a new food / beverage composition that can be used as a food is desired!

[0016] Also, in the composition that also has the power of yucca extract and quilla extract, the price is not low, so that it has been required to have a sufficient blood cholesterol lowering effect even with a smaller amount of food intake. . Therefore, there has been a demand for the development of a new composition that enhances the blood cholesterol lowering effect of a composition that also has the power of a yucca extract and a killer extract, and is safe and can be used as a food or drink.

[0017] Therefore, in view of the situation of the prior art described above, the present invention effectively improves the gastrointestinal tract function of a composition that effectively lowers blood cholesterol and blood triglycerides and at the same time has the ability to extract yucca and killer. The purpose is to provide a composition and food and drink that can be synergistically enhanced.

Means for solving the problem

[0018] As a result of diligent research on the food and drink materials that can enhance the gastrointestinal function improving action and lipid (cholesterol, triglyceride) metabolism improving action of the composition having the power of yucca and quilla extract, The inventors found that lactic acid bacteria have such an action and completed the present invention.

[0019] Further, the present invention has been found that a composition having the extractable power of yucca and quilla can promote the growth of lactic acid bacteria and enhance the gastrointestinal function improving action and lipid (cholesterol, triglyceride) metabolism improving action of lactic acid bacteria. It came to complete. [0020] The composition or food / beverage product according to the present invention is composed mainly of a composition comprising yucca and quilla extract and lactobacilli. That is, a composition obtained by blending and mixing lactic acid bacteria with a composition having the power of yucca and quilla extract, and a food and drink obtained by further blending and processing various food and drink ingredients.

[0021] Examples of lactic acid bacteria that can be used in the present invention include Lactobacilli for fermentation (for example, Lactobacillus delbrueckn subsp. Bulgancus, Lactobacillus delbrueckn subsp. Lactis, Lactobacillus helveticus, Lactobacillus helveticus subsp. Jugurti), , Streptococcus thermophilus, Lactococcus lactis subsp. Lactis, Lactococcus lactis subsp.cremoris, Lactococcus deacetilactis), Leuconostoc cremoris , Lactobacillus gallmarum, Lactobacillus gassen, Lactobacillus johnsoniu Lactobacillus casei, Lactobacillus casei subsp.rhamnosus), moon cake tuber Lactococcus Lactococcus (for example, Enterococcus faecium, Streptococcus faecium,

Enterococcus faecalis, Streptococcus faecalis), bifido spear, e.g.

Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve ^

Bifidobacterium infantis ゝ Bifidobacterium adolescentis), spores' sex bacterium L acid bacteria (e.g. Bacillus coagulans, Lactobacillus sporogenes), plant '' sex moth L acid bacteria (e.g.

Lactobacillus plantarum, Pediococcus halophilus), and other pronoquatic 孚 L acid bacteria (for example, Lactobacilus reuteri, Lactobacillus rhamnosus ^ Lactobacillus salivarius, Lactobacillus hilgardii, Lactobacillus paralimentarius, Enterococcus faecalis, Enterococcus faecalis, etc.) One or more of these lactic acid bacteria are mixed and used together. Lactic acid bacteria can be used in a wet state collected by centrifugation and filtration, for example, but it is preferable to use freeze-dried cells or freeze-dried cells using a freeze-dried cell or lyophilization aid, etc. .

[0022] Compositions comprising yucca and quilla extracts are described in PCT / KR01 / 00299 and SW Kim et al, Arch. Pharm. Res., Vol. 26, No. 12, pp. 1042-1046, 2003. The disclosed composition (a composition containing 0.001% by weight or more of each of yucca and quilla extracts on a solid basis) can be given as a representative example. Between yucca extract and quilla extract The blending ratio is preferably 90: 10—10: 90! /, But more preferably 80: 20—20: 80.

[0023] As the form of food, it can be a solid supplement prepared in a usual form such as tablets, coated tablets, capsules, parcels, powders, powder products, condyles, sticks, three-way seals.

Yogurt may also be used, and the lactic acid bacteria in this case are produced using one or more of Bulgarian bacteria, Thermophilus bacteria, Acidophilus bacteria, Bifidobacterium, and the like. More specifically, plain-type yogurt, hard-type yogurt, soft-type yogurt, drink-type yogurt and frozen-type yogurt may be used, and so-called caspian sea yogurt and kefir yogurt may be used. Furthermore, it can also be used as a lactic acid bacteria beverage such as Canolebis (trademark), Kakult (trademark), fermented milk (acidic milk). Liquid products can be solutions, suspensions, emulsions, syrups and the like. It can also be made into ice cream containing lactic acid bacteria.

[0024] Examples of lactic acid bacteria include the genus Bifido (eg, Bifidobacterium bifidum, Bifidobacterium longum, Bindobactenum breve ^ Bifidobacterium infantis, Bindobactenum

adolescentis) or a mixture of two or more of them may be used, and lactic acid bacteria other than the genus Bifidos and lactic acid bacteria of the genus Bifidos may be used in combination. As the genus Bifido, for example, Morinaga bifidobacteria powder (BB536) manufactured by Morinaga Milk Co., Ltd., acid-resistant freeze-dried bifidobacteria powder (FD bifido ATK) manufactured by Kyowa Hakko Co., Ltd., and the like can be used.

[0025] Preference food materials such as natural fruit juice, coffee, cacao, mineral, carbonic acid, alcohol and the like can be appropriately added to lactic acid bacteria beverages, yogurt, ice cream and the like. The types and addition amounts of these materials are not particularly limited as long as they do not inhibit the lactic acid bacteria growth promoting action, blood cholesterol reducing action, blood triglyceride reducing action, etc. by the food of the present invention. , Organic acids, peptides, proteins, oligosaccharides, isoflavones, catechins, lipids, minerals, yeast extracts, propolis, chlorella, spirulina, herbs, vegetables 'fruit' and other natural products Appropriate amounts such as extracts, processed products such as egg yolk and egg white, coenzymes, enzymes, useful microorganisms (live and dead), and microorganism-derived products can be used. [0026] In these lactic acid bacteria beverages, yogurt, ice cream, etc., coloring agents, sweeteners, flavorings, preservatives, antioxidants, emulsifiers, thickeners, quality improvers, A seasoning, a sour agent, a strengthening agent, etc. can be mix | blended and added suitably.

[0027] In the case of a solution beverage such as yogurt, lactic acid bacteria beverage, fermented soymilk by lactic acid bacteria, etc., after adding or mixing the composition capable of extracting yucca and quilla to milk or soymilk, one or more kinds Yogurt and fermented soymilk may be produced using lactic acid bacteria, or a composition comprising yucca and quilla extract in yogurt and fermented soymilk produced using one or more lactic acid bacteria. Saddle · Can be mixed.

[0028] In the case of yogurt, for example, it is also preferable to produce yogurt with Streptococcus 'Thermophyllus and Lactobacillus' Bulgaricus and add bifidobacteria to it. In that case, bifidobacteria can be added and mixed as yogurt having a pH of about 5 or more.

[0029] A composition comprising a yucca extract and a quilla extract used in the product of the present invention, such as a liquid product such as a drink, a semi-liquid product such as yogurt, and a solid product such as a solid supplement, is based on a solid content. In each case, 0.0 to 95% by weight can be contained, but 0.1 to 80% by weight is preferable.

[0030] When using Enterococcus faecalis (Streptococcus faecalis), Lactobacillus acidophilus, Lactobacillus bulgaricus, these live cells are collected and dried, and then starch, lactose, sucrose, The use of extra-standard lactamine mixed with a suitable excipient such as a mixture of

[0031] As a spore-forming lactic acid bacterium (Ratatobacillus 'sporogenes;Bacillus' coagulance), lacpon bulk powder can be used frequently. Ratabon bulk powder ^{typically, 5 X 10 9 - 12 X} 10 12 cells / g powder with containing the number of bacteria can be used. In order to promote the growth of spore-forming lactic acid bacteria, it is also preferable to use Takadiasterase (trademark), piotin (vitamin H) or a combination of both. Preferred examples thereof include about 3 g of Takadastase N1 and about 0.4 mg of piotin for 5 × 10 ⁹ —12 × 10 ¹² spores of lactic acid bacteria.

[0032] Also, for example, excipients (eg, starch, lactose, microcrystalline cellulose, cellulose, calcium hydrogen phosphate, sugar), dispersing or binding agents (eg, calcium carbonate, silicon dioxide) , Polyvinylpyrrolidone, methylcellulose, carboxymethylcellulose, gum arabic, hydroxypropylcellulose, hydroxypropylmethylcellulose phthalate, titanium oxide), emulsifier (eg glycerin fatty acid ester, sucrose fatty acid ester, lecithin, alkyl sulfonate, aryl sulfonate), lubrication Agents (eg, magnesium stearate, talc, stearic acid, magnesium aluminate metasilicate, sodium lauryl sulfonate, rapeseed oil powder), other adjuvants (eg, vegetable oils such as water, norafin, peanut oil and sesame oil, Produced in the usual way by adding alcohol such as ethanol or glycerin, or glycol such as propylene glycol or polyethylene glycol) Rukoto can. The amount of applied force is preferably 5 to 80%, which is usually 0 to 90%.

The invention's effect

[0033] A composition comprising the yucca and quilla extract of the present invention and a lactic acid bacterium or a food or drink containing the same composition is a conventional food or drink containing the yucca and quilla extract, or Compared to foods and drinks containing lactic acid bacteria, it has the following effects.

[0034] Excellent gastrointestinal function improving action and lipid metabolism improving action are exhibited. This is a result of the complementary or synergistic enhancement of the gastrointestinal function improving action and lipid metabolism improving action of the composition comprising yucca and quilla extract, and the gastrointestinal function improving action and lipid metabolism improving action of lactic acid bacteria. I think that the.

[0035] Compared to the case where a composition comprising yucca and quilla extract and lactic acid bacteria are each used alone, blood cholesterol can be lowered more efficiently, and blood triglycerides can be lowered. be able to.

[0036] Further, the present invention includes the above-described blood triglyceride lowering action, the function of inhibiting fat absorption of the yarn and composition comprising yucca and quilla extract, and the intestinal action of lactic acid bacteria. It is suggested that the anti-obesity effect is enhanced by food and beverage inoculation, and a better diet effect can be expected.

BEST MODE FOR CARRYING OUT THE INVENTION

[0037] The present invention is a method of blending and mixing lactic acid bacteria with a composition comprising yucca and quilla extract, and further blending and processing various food and drink ingredients. The following production examples and test examples are described below. The present invention will be described in detail with reference to the following.

[0038] [Production Example 1]

Yucca extract (40%) and Kira extract (60%) Mix well (350g) and lactose (330g). And granulated by spraying water under an incoming air pressure of 2 kg / cm ² . Spraying was carried out for 15 minutes at a flow rate of 5 ml / min and a pressure of 0.8–0.85 kg / cm ² . After drying for about 8 minutes under an intake air temperature of about 80 ° C, it was passed through a 50 m sieve and granulated through a sizing process. Bifidobacteria powder (6 g), lactamine (Fukaris bacteria), lactamine (Lactobacillus acidophilus) and magnesium stearate (4 g) were mixed with this, and using a tablet press (AP22SS-U) manufactured by Hata Iron Works, According to a conventional method, the tablet was 210 mg / 1 tablet.

[0039] [Production Example 2]

Yucca extract (40%) and quilla extract (60%) The composition (600 g) consisting of strength and lactose (400 g) were mixed well and granulated by the same method as in Production Example 1. Bifidopacterium 'Longum fungus powder (24g), Bifidobatterium' Bifidum fungus powder (24g), Lactobacillus acidophilus fungus powder (24g), corn starch (122g) and magnesium stearate ( 6 g) was mixed, and 300 mg / l tablet was prepared by a conventional method using a tableting machine (AP22SS-U) manufactured by Hata Iron Works.

[0040] [Production Example 3]

Yucca extract (40%) and quilla extract (60%) composition (300g), catechin extract powder (300g), bihud bataterum 'longum fungus powder (24g), bifud bataterum' bihuidum Mixing mycelium (24g), Lactobacillus acidophilus fungus (24g), lactose (188g), dextrin (100g), cellulose (180g), glycerin fatty acid ester (36g), and diacid salt (24g) Then, using a tablet press (AP22SS-U) manufactured by Hata Iron Works, it was made into a tablet of 300 mg / l tablet by a conventional method.

[0041] [Production Example 4]

Yucca extract (40%) and quilla extract (60%) composition (400g), catechin extract powder (450g), bihud bataterum 'longum fungus (24g), bifud bataterum' bihuidum Mycelium (24g), Lactobacillus acidophilus fungus (24g), Fenugreek endosperm powder (96g), lactose (107g), dextrin (150g), cellulose (150g), glycerin fatty acid ester (75g) Using an ironworks tableting machine (AP22SS-U), in a conventional manner, with 300 mg / l tablets did.

[0042] [Production Example 5]

Composition of daily intake (1.5g) Yucca extract (60%) and Kirya extract (40%) composition (700mg), latavone (spore-forming lactic acid bacteria) (10mg), lactamine (Lactobacillus acidophilus) ) (18 mg), acid-free lyophilized bifidobacteria powder (FD bifidotus ATK) (18 mg), natural oligosaccharide raffinose (170 mg), piotin g), lactose (234 mg) and potato starch (350 mg). These components were added and mixed uniformly to obtain a powder.

[0043] [Production Example 6]

The daily intake (3g; l-pack 1.5g stick-like nutritional health food) is composed of yucca extract (70%) and quilla extract (30%). Spore-forming lactic acid bacteria (5mg), Lactamine (Lactobacillus acidophilus) (10mg), Bifido Batterium 'Longham fungus powder (10mg), Bifido Batteratum. (10 mg), fructooligosaccharide (100 mg), vitamin C (62.5 mg), citrate (62.5 mg), granulated sugar (400 mg), corn starch (240 mg) and lactose (240 mg). In addition, it was mixed evenly into a stick-shaped product of 1.5g per bag.

[0044] [Production Example 7]

Lactamine (Lactobacillus acidophilus) was inoculated into a yogurt mix (raw milk was skimmed with 2% skim milk and heated at 100 ° C for 15 minutes) and cultured at 20 ° C for 24 hours. 150g of prepared yogurt, yucca extract (30%) and quilla extract (70%) composition (750mg), furato-oligosaccharide (200mg), latavone (spore-forming lactic acid bacteria) (10mg), acid-resistant freeze Dried bifidobacterial powder (FD bifido ATK) (10 mg) and piotin (1 μg) were added and mixed, filled into a paper cup, cooled and used as a product.

[0045] [Production Example 8]

The skim milk was sterilized at 80-85 ° C for 20-30 minutes and then homogenized. This was inoculated with ratatomin (Lactobacillus acidophilus) and fermented at 37-40 ° C for 20 hours. The resulting curd was crushed and cooled to 5 ° C, which was used as fermented milk. Separately, sugar (20%), yucca extract (40%) and killer extract (60%) composition consisting of coconut (0.6%), ratavon (spore-forming lactic acid bacteria) (0.04%), acid-freezing freezing Dried bifidobacteria powder (FD bifidotus ATK) (0.04%), lactamine (Lactobacillus acidophilus) (0.04%) Piotin (2 g), furato-oligosaccharide (0.6%), appropriate amounts of acidulant and fragrance were added, homogenized, cooled to 5 ° C, and used as a sugar solution. An equal amount of the fermented milk and sugar solution thus obtained were mixed to obtain a lactic acid bacteria beverage.

[0046] [Production Example 9]

Thoroughly wash the container of Yogurt Maker manufactured by ZOJIRUSHOBIN Co., Ltd., disinfect with hot water, add milk (500 ml), and separately make Yucca extract (40%) and Kira extract (60%) The product (lg) was dissolved in 5 ml of water, and an aqueous solution sterilized by heating at about 95 ° C. for 15 minutes was added. In addition, 3 tablespoons of powdered inoculum of Caspian Sea yogurt (sold on food safety and health network) was added. After mixing with a spoon sterilized with hot water, it was allowed to stand at 27 ° C for 15 hours to obtain a product.

[0047] [Production Example 10]

Yakult (65 ml) was taken out of the refrigerator and left at room temperature to approach room temperature. About half of the apples were grated and squeezed with an exposed cloth to prepare apple juice. Separately, skim milk (60 g) was mixed with sugar (20 g) and mixed well. Mix apple juice with water to make 500ml, mix with skim milk and sugar, and lightly mix the yucca extract (40%) and quilla extract (60%) composition (900mg). Add and dissolve. Heat to boiling, and after boiling, heat sterilize for 10 minutes using low heat. The boiling liquid was put in a bowl filled with water, rapidly cooled to about 35 ° C, and transferred to a heat-retaining water bottle. Yakult (including Ratatobacillus 'casei' Shirota strain) (65 ml) was prepared and fermented for about 10 hours to obtain a product.

[0048] [Test Example 1]

MRS medium for lactic acid bacteria (Nippon Betaton Dickinson Co., Ltd.) 5.2 g was dissolved in 90 ml of water, dispensed into 6 test tubes (9 ml), and sterilized at 121 ° C for 15 minutes. One bottle was covered with lml water. Separately, 3 ml of 1%, 3%, 6%, 12%, 20% aqueous solution of yucca and kiraja extract (Yucca extract: kiraja extract = 45.5: 54.5) is prepared with a 0.22 m filter. Aseptically filtered. This yucca and killer extract extract composition (lml) is added to the other five test tubes and consists of 0.1%, 0.3%, 0.6%, 1.2%, 2% yucca and killer extract. A containing medium was prepared.

In these test tubes, separately, the bifido butterium 'longa cultured as shown in Note 1 below. 100 μl of Bifidobacterium longum fungus was inoculated. Take 200 μ1 of each test tube and use 100 / ζ 1 to measure the number of lactic acid bacteria (Bifidobatterium 'longum), and the average value is taken as the number of lactic acid bacteria before cultivation (see Note 2) . The remaining 100 1 was used to measure ρΗ. Further, after anaerobic culture at 37 ° C. for a certain period of time, 200 1 was taken and 100 1 was used to measure the number of lactic acid bacteria, and the remaining 100 1 was used to measure pH and the number of viable bacteria over time. Lactic acid bacteria is 10 ⁵ an anaerobic dilution takes 100 1 of broth (see Note 3) - 100 mu 1 of the solution was diluted to 10 ⁸ on plates of BL agar (manufactured by two Ssui Ltd.) After application using cornage and anaerobic culture at 37 ° C for 72 hours, the number of colonies grown was counted. The number of viable lactic acid bacteria on 3 BL agar plates per sample was counted, and the average value was calculated as the number of lactic acid bacteria. The results are shown in Table 1.

Note 1: 10 ml of a solution of 5.2 g of MRS medium for lactic acid bacteria in 100 ml of water was sterilized at 121 ° C for 15 minutes. Stored frozen (-80 ° C) in an aqueous solution containing skim milk (10%) and sodium glutamate (1%)! / Bifidobatterium longum was streaked anaerobically on BL2 agar medium. One platinum loop of the resulting colony was inoculated and anaerobically cultured at 37 ° C for 18 hours.

Note 2: The number of lactic acid bacteria before cultivation in this experiment was 1.8 × 10 ⁷ cells / ml, and the pH was 6.6.

Note 3: Anaerobic Diluent: 1 liter of water, 1 potassium phosphate (4.5 g), 2 sodium phosphate (6.0 g), agar (0.5 g), twin 80 (0.5 g), cysteine hydrochloride (0.5 g) was dissolved and the pH was adjusted to 7.0.

Table 1. Growth-promoting effect of yucca and quilla extract on bifidobatterium 'longum

Yucca and quilla extraction Culture time (pH viable count / ml (puffed bacteria addition amount (%) time)-Longham)

3 6.3 8.0X10 ⁶

6 6.4 1.4X10 ⁷

0 9 6.2 6.0X10 ⁷

12 4.7 1.6X10 ⁸

24 4.6 3, 6X10 ⁸

3 6.3 1.3X10 ⁷

6 6.3 2.0X10 ⁷

0.1 9 6.1 1, 2X10 ⁸

12 4.6 8.0X10 ⁸

24 4.3 1, 9X10 ⁹

3 6.3 1.7X10 ⁷

6 5.9 2.4X10 ⁷

0.3 9 5.7 2, 4X10 ⁸

12 4.6 1, 2X10 ⁹

24 4.3 3, 0X10 ⁹

3 6.2 1.9X10 ⁷

6 5.6 4, 0X10 ⁷

0.6 9 5.4 5.2X10 ⁸

12 4.6 2, 2X10 ⁹

24 4.3 5, 6X10 ⁹

3 6.2 4.0X10 ⁷

6 5.6 6.0X10 ⁷

1.2 9 4.9 1, 0X10 ⁹

12 4.5 4, 2X10 ⁹

24 4.3 2, 2X10 ⁹

3 6.1 5.0X10 ⁷

6 5.4 1, 0X10 ⁸

2.0 9 4.8 1.6X10 ⁹

12 4.5 4, 6X10 ⁹

24 4.3 1.6X10 ^{9 From} Table 1, it is clear that the composition consisting of Yucca and Quillaja extract significantly promotes the growth of bifidobacterum longum. In addition, when the composition with the extract of Yucca and Kira extract is not added, it is the force that the viable count decreases after 3 hours of cultivation. Because it is not a medium suitable for growth, the growth is active in the induction phase before entering the logarithmic growth phase! When a composition that also has the extract of yucca and quilla is added, there is almost no decrease in the number of viable cells after 3 hours even with addition of 0.1% -0.6%, and growth with addition of 1.2% -2.0% It could be confirmed. This is also the strength of yucca and quilla extract. Is a composition suitable for the growth of Bifidobacterium. With the addition of yucca and quilla extract 1.2% and 2.0%, a decrease in the number of viable cells was observed in 24 hours of culture. This is because the growth promoting effect of yucca and quilla extract is large, and the growth rate is faster. It is thought that after some time, some bihuid butterium 'Longham is in the death period.

[Test Example 2]

Thirty five-week-old BALB / C aseptic mice reared on CL2 diet (manufactured by CLEA Japan) were used, and two groups (15 mice each): a sterile mouse group and a bifid baterium 'longum colonized mouse group It was divided. Bifido Batterium 'Longam colonized mice were created as follows.

At the time of breeding for 5 weeks, 0.5 ml of the bacterial solution of bifidobatterium 'longum prepared as in Note 1 below was orally administered only once. By this administration, Bifido Batterium longum was established in sterile mice. Confirmation of bifid butterium 'Longham's colonization was conducted as described in Note 2. The animals were further bred for 1 week on CL2 feed for a total of 6 weeks.

Thereafter, they were raised on radiation-sterilized cholesterol feed (see Note 3), and at the same time, administration of a composition consisting of yucca and quilla extract (yucca extract: kiraja extract = 45.5: 54.5) was also started. Yarn and quilla extract and thread administration were given in such a way that mice were free to drink at 0.5% and 1.0% concentrations in drinking water.

The control group (sterile mouse group) is a non-administration group of a composition that also has yucca and quilla extract strength, a group that is administered a composition of 0.5% yucca and quilla extract strength, and a composition administration consisting of 1.0% yucca and quilla extract strength The group was divided into 3 groups (5 animals in each group). The bifudacterium 'longumum constitutive group is also composed of a non-administration group of a composition comprising a yucca and quilla extract, a yarn and a compound administration group comprising 0.5% yucca and quilla extract, and a 1.0% yucca and quilla extract group. The group was divided into 3 groups (5 mice each).

They were raised on a cholesterol diet for 4 weeks, during which time YQ2 was given freely from drinking water. After feeding on a cholesterol diet, blood triglycerides and blood total cholesterol were measured at 4 weeks. As a result, blood triglycerides and blood cholesterol were significantly different between the aseptic mouse group and the bifidobatterium 'longum colonization group compared with the non-added group of yucca and quilla extract yarn and adults. I helped. That is, Bifido Batterium 'Longam alone The effectiveness in was not recognized.

However, when administered in combination with the composition of yucca and quilla extract in the bifid butteryrum 'longum colonization group, the addition of the yucca and quilla extract composition, and There was a statistically significant difference at p <0.01 for the sterile mouse group. The results are shown in Table 2. Table 2 shows the reduction rate of blood triglycerides and blood cholesterol from the initial values.

Note 1: 10 ml of a solution of 5.2 g of MRS medium for lactic acid bacteria in 100 ml of water was sterilized at 121 ° C for 15 minutes. This was infused with Bifido Batterium longum and anaerobically cultured at 37 ° C for 18 hours. Note 2: The bifidobatterium longum was measured and confirmed after 1 week, 2 weeks, and 4 weeks after bifidobatterium longum administration.

Note 3: The composition of mouse cholesterol diet is corn starch (50.4486%), milk casein (20.0%), pregelatinized corn starch (1.0%), granulated sugar (10.0%), purified lard (7.0%), and avicel cellulose (5.0%). %), Mineral mixture (AIN-93G-MX; 3.5%), vitamin mixture

(AIN-93VX; 1.0%), L-cystine (0.3%), choline bitartrate (0.25%), tert-butylhydroquinone (0.0014%), cholesterol (1.0%), and sodium cholate (0.5%).

[0052] Table 2. Synergistic lowering effect of blood composition consisting of yucca and quilla extract and bifid bataterum 'longum on blood triglycerides and blood cholesterol

[0053] [Test Example 3]

The growth promoting effect of a composition composed of yucca and quilla extract against various lactic acid bacteria (Yucca extract: kiraja extract = 40: 60) was examined. The composition (w / v%) of the growth medium for various lactic acid bacteria and bifidobacteria used was meat extract (OXOID) (0.26%), proteose peptone (BD) (1.0%), yeast extract (BD) (0.5% ), Sodium monohydrogen phosphate (0.4%), glucose (1.0%), soluble starch (0.05%), L-cystine (0.02%), L-cystine hydrochloride (0.05%), twin 80 (0.05%) PH 7.0. The test medium is a liquid medium for growth (10 ml) supplemented with a composition consisting of an extract of yucca and quilla at 0.1%, 0.3%, 0.6%, 1.2%, 2.0%, and 121 ° C. Sterilized for 15 minutes. A liquid medium supplemented with a composition consisting of yucca and quilla extract was sterilized at 121 ° C. for 15 minutes and used as a control. The lactic acid bacterium used was a non-La bacillus casei.

JCM1134 (Lactobacillus casei JCM1134), Lactobacillus acidophilus

JCM1132 (Lactobacillus acidophilus JCM1132), Lactobacillus plantaran

No.l94 (Lactobacillus plantarum No.194), Bifido Batterium 'Longam

WB 1001 (Bifidobacterium longum WB1001), Bifido Batterium 'breve

JCM1273 (Bifidobacterium breve JCM1273), bifidobatterium 'adrecentis JCM1275 (Bifidobacterium adlescentis JCM1275). Inoculate 0.1 ml of each strain anaerobically cultured at 37 ° C for 18 hours in a liquid medium for growth (121 ° C, sterilized for 15 minutes) in advance. The degree was measured. Turbidity was measured at 550 nm by adding the sampled solution to a 96-well microplate. The results are shown in Table 3-8. Table 3. Growth-promoting effects of yucca and quilla extract on Lactobacillus casei JCM1134 (row indicates% addition of YQ2, column indicates culture time)

Table 4. Growth-promoting effect of Yucca and Quillaja extract on Lactobacillus acidophilus JCM1132 (row indicates% addition of YQ2, column indicates culture time) 0% 0. 1% 0. 3% 0. 6% 1. 2% 2. 0%

0 (Time) 0 0 0 0 0 0

3 (Time) 0. 04 0. 045 0. 068 0, 026 0. 031 0. 036

6 (Time) 0. 142 0. 112 0. 131 0, 104 0. 105 0. 076

9 (hours) 0. 203 0. 175 0. 196 0, 213 0. 224 0. 211

12 (hours) 0. 196 0. 163 0. 236 0, 255 0. 259 0. 238

24 (hours) 0. 187 0. 201 0. 236 0, 234 0. 285 0. 307

Table 5. Growth-promoting effect of Yucca and Quillaja extract on Lactobacillus' plantaran No. 194 (row indicates% addition of YQ2, column indicates culture time)

Table 6. Growth-promoting effect of yucca and quilla extract on bifluid bataterum 'longum WB1001 (row indicates% addition of YQ2, column indicates culture time)

Table 7. Growth-promoting effect of yucca and quilla extract on bifidobatterium breve JCM1273 (row indicates% addition of YQ2, column indicates culture time) 0% 0. 1% 0. 3% 0. 6% 1. 2% 2. 0%

0 (Time) 0 0 0 0 0 0

3 (Time) 0. 029 0. 035 0. 032 0, 042 0. 049 0. 056

6 (Time) 0. 1 1 0. 19 0. 258 0, 228 0. 263 0. 233

9 (hours) 0. 349 0. 289 0. 3 0, 345 0. 429 0. 48

12 (hours) 0. 348 0. 302 0. 293 0, 326 0. 379 0. 447

24 (hours) 0. 363 0. 343 0. 381 0, 407 0. 391 0. 433

[0059] Table 8. Growth-promoting effect of Yucca and Kira extract on Bifidobacterium 'adrecentis JCM1275 (row indicates% addition of YQ2, column indicates culture time)

[0060] Tables 3 to 8 show that the composition of yucca extract and killer extract has a universal growth-promoting effect on many lactic acid bacteria in a concentration-dependent manner.

[0061] [Test Example 4]

The growth promoting effect of a composition composed of yucca and quilla extract against sporic lactic acid bacteria (Yucca extract: quilla extract = 60:40) was examined. The spore-forming lactic acid bacteria used were Bacillus coagulans TI 1006, and the growth medium was Trypticase Soy Broth. The test medium was added to the liquid medium for growth (10 ml) by adding 0.1%, 0.3%, 0.6%, 1.2%, and 2.0% of the yarn and composition composed of yucca and quilla extract to 121 ° C. Sterilized for 15 minutes. The yucca and quilla extract-strength composition was sterilized at 121 ° C for 15 minutes in a liquid medium containing no added calories and used as a control. Pre-inoculate 0.2 ml of each strain aerobically cultured at 37 ° C for 24 hours using a liquid medium for growth (121 ° C, sterilized for 15 minutes) in a test solution (10 ml). Sampling was performed to measure turbidity and pH. Turbidity was measured at 550 nm by adding the sampled solution to a 96-well microplate. Table 9 shows the results. It was shown to.

[0062] Table 9. Growth-promoting effects of yucca and quilla extracts on Bacillus coagulance TI 1006 (rows indicate% addition of YQ2 and columns indicate culture time)

Table 9 shows that the yucca extract and the quilla extract extract also have a growth-promoting action on Bacillus coagulance TI 1006 in a concentration-dependent manner.

Industrial applicability

[0063] Since the food and drink according to the present invention has an enhanced blood cholesterol lowering action, it prevents and treats hyperlipidemia, prevents and treats arteriosclerosis causing myocardial infarction and cerebral infarction, and stroke. It is useful as a composition or food or drink suitable for the above.

[0064] Further, since it has a blood triglyceride lowering effect, it not only leads to prevention and treatment of hyperlipidemia, but is also useful as a composition or food and drink suitable for anti-obesity.

Claims

The scope of the claims

[1] A composition comprising a yucca extract, a quilla extract, and a lactic acid bacterium, and has a function of promoting the growth of lactic acid bacteria and a function of reducing blood triglycerides.

[2] The composition according to claim 1, wherein the mixing ratio of the yucca extract and the quilla extract is 90: 10—10: 90.

[3] A food or drink comprising a composition comprising a yucca extract, a quilla extract, and lactic acid bacteria, and having a function of promoting the growth of lactic acid bacteria and a function of reducing blood triglycerides

P

PPo

[4] The food / beverage product according to claim 3, wherein the mixing ratio of the yucca extract and the quilla extract is 90: 10—10: 90.

[5] The food or drink according to claim 3, wherein the food or drink is a solid supplement.

[6] The food or drink according to claim 3, wherein the food or drink is yogurt.

[7] The food or beverage according to claim 3, wherein the food or beverage is a lactic acid bacteria beverage.

[8] The food and drink according to claim 3, wherein the lactic acid bacterium containing bifidobacteria is used.